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Gene Review

RPA3  -  replication protein A3, 14kDa

Homo sapiens

Synonyms: REPA3, RF-A protein 3, RP-A p14, RPA14, Replication factor A protein 3, ...
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Disease relevance of RPA3

  • Assembly of vaccinia virus: incorporation of p14 and p32 into the membrane of the intracellular mature virus [1].
  • There have been only three reported cases of anti-RPA in systemic lupus erythematosus (SLE) and Sjögren syndrome (SjS) [2].
  • We report that during activation of the simian virus 40 (SV40) pre-replication complex, SV40 T antigen (Tag) helicase actively loads replication protein A (RPA) on emerging single-stranded DNA (ssDNA) [3].
  • Using a polyclonal antibody against recombinant p14, we show here that p14 is also localized to the nucleoli of murine mammary carcinomas and some human breast cancer samples [4].
  • We have previously shown that the leader peptide (p14) of the Env-precursor of mouse mammary tumor virus is translocated into the nucleoli of murine T cell lymphomas that harbor this virus [4].

High impact information on RPA3


Biological context of RPA3


Anatomical context of RPA3

  • Our data are consistent with a model whereby p32 would become inserted into cellular membranes before being incorporated into the crescents whereas p14 would be posttranslationally associated with the viral outer membrane at a specific later stage of the viral life cycle [1].
  • These data suggest that the p14 FAST protein associates with heterogeneous membrane microdomains, a distinct subset of which is defined by cholesterol-dependent Lubrol WX resistance and which may be more relevant to the membrane fusion process [9].
  • We analyzed the lipid environment in which the reptilian reovirus p14 FAST protein resides to determine the influence of the cell membrane on the fusion activity of the FAST proteins [9].
  • The volume estimates in newborn and p14 mice suggest that the overgrowth in mceph/mceph hippocampus starts between birth and the second week of life [10].
  • The observation that COS cells transformed with a cDNA encoding zeta express zeta-p14 supports the former possibility [11].

Associations of RPA3 with chemical compounds

  • RPA 3 was inhibited by N-acetyl-D-galactosamine and human alpha-glycoproteins [12].
  • From SDS/polyacrylamide-gel-electrophoresis data it was estimated that RPA 1 consisted of two subunits of Mr 34,000, and RPA 3 of two types of subunits (Mr 30,500 and 29,000) [12].
  • In transfected cells, p14 was found in both Lubrol WX- and Triton X-100-resistant membrane complexes [9].

Physical interactions of RPA3

  • The complex of hRPA14 and hRPA32-(43-171) in turn formed a trimeric complex with the C-terminal region of hRPA70 (amino acids 436-616) [13].

Other interactions of RPA3

  • Limited proteolysis of the hRPA14.32 complex defined a core dimer composed of the central region of hRPA32 (amino acids 43-171) and RPA14 [13].
  • It is composed of three subunits of 70, 34, and 13 kDa (Rpa1, Rpa2, and Rpa3, respectively) [14].
  • Among the nine polypeptides detected by immunoblotting, 38 recognized p14, 17 recognized p8, and 16 recognized p8, and 16 recognized p30 [15].

Analytical, diagnostic and therapeutic context of RPA3

  • By analytical ultracentrifugation the Mr values of RPA 1 and RPA 3 were estimated to be 59,000 and 105,000 respectively [12].
  • Antigen-capture ELISA with anti-RPA32 mAb, immunofluorescent antinuclear antibodies (ANA) and western blot analysis with purified RPA were also performed [2].
  • Topographical mapping of the surface of fusogenic p14-containing liposomes by atomic force microscopy under aqueous conditions revealed that p14 resides almost exclusively in thickened membrane microdomains [9].
  • Perfusion images recorded in the acute stage after RFA showed higher compliance to the definitive ablation volume reached after 6 weeks than T2-weighted images, which underestimated the true necrosis size [16].
  • For the assessment of the extent of coagulation and for the detection of residual tumor, T1-weighted gradient-echo imaging, T2-weighted spin echo imaging and two different perfusion imaging techniques were performed before, 1 day and 6 weeks after RFA at 1.5 T [16].


  1. Assembly of vaccinia virus: incorporation of p14 and p32 into the membrane of the intracellular mature virus. Sodeik, B., Cudmore, S., Ericsson, M., Esteban, M., Niles, E.G., Griffiths, G. J. Virol. (1995) [Pubmed]
  2. Autoantibodies against the replication protein A complex in systemic lupus erythematosus and other autoimmune diseases. Yamasaki, Y., Narain, S., Hernandez, L., Barker, T., Ikeda, K., Segal, M.S., Richards, H.B., Chan, E.K., Reeves, W.H., Satoh, M. Arthritis Res. Ther. (2006) [Pubmed]
  3. Structural mechanism of RPA loading on DNA during activation of a simple pre-replication complex. Jiang, X., Klimovich, V., Arunkumar, A.I., Hysinger, E.B., Wang, Y., Ott, R.D., Guler, G.D., Weiner, B., Chazin, W.J., Fanning, E. EMBO J. (2006) [Pubmed]
  4. Mouse mammary tumor virus Env-derived peptide associates with nucleolar targets in lymphoma, mammary carcinoma, and human breast cancer. Bar-Sinai, A., Bassa, N., Fischette, M., Gottesman, M.M., Love, D.C., Hanover, J.A., Hochman, J. Cancer Res. (2005) [Pubmed]
  5. Replication protein A: a heterotrimeric, single-stranded DNA-binding protein required for eukaryotic DNA metabolism. Wold, M.S. Annu. Rev. Biochem. (1997) [Pubmed]
  6. A novel human primary immunodeficiency syndrome caused by deficiency of the endosomal adaptor protein p14. Bohn, G., Allroth, A., Brandes, G., Thiel, J., Glocker, E., Sch??ffer, A.A., Rathinam, C., Taub, N., Teis, D., Zeidler, C., Dewey, R.A., Geffers, R., Buer, J., Huber, L.A., Welte, K., Grimbacher, B., Klein, C. Nat. Med. (2007) [Pubmed]
  7. High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3). Umbricht, C.B., Griffin, C.A., Hawkins, A.L., Grzeschik, K.H., O'Connell, P., Leach, R., Green, E.D., Kelly, T.J. Genomics (1994) [Pubmed]
  8. Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A. Umbricht, C.B., Erdile, L.F., Jabs, E.W., Kelly, T.J. J. Biol. Chem. (1993) [Pubmed]
  9. The p14 Fusion-associated Small Transmembrane (FAST) Protein Effects Membrane Fusion from a Subset of Membrane Microdomains. Corcoran, J.A., Salsman, J., de Antueno, R., Touhami, A., Jericho, M.H., Clancy, E.K., Duncan, R. J. Biol. Chem. (2006) [Pubmed]
  10. Lack of potassium channel induces proliferation and survival causing increased neurogenesis and two-fold hippocampus enlargement. Almgren, M., Persson, A.S., Fenghua, C., Witgen, B.M., Schalling, M., Nyengaard, J.R., Lavebratt, C. Hippocampus (2007) [Pubmed]
  11. Activation-induced proteolysis of cytoplasmic domain of zeta in T cell receptors and Fc receptors. Taupin, J.L., Anderson, P. Eur. J. Immunol. (1994) [Pubmed]
  12. Purification and characterization of Robinia pseudoacacia seed lectins. A re-investigation. Wantyghem, J., Goulut, C., Frénoy, J.P., Turpin, E., Goussault, Y. Biochem. J. (1986) [Pubmed]
  13. The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain. Bochkareva, E., Frappier, L., Edwards, A.M., Bochkarev, A. J. Biol. Chem. (1998) [Pubmed]
  14. Dissection of functional domains of the human DNA replication protein complex replication protein A. Lin, Y.L., Chen, C., Keshav, K.F., Winchester, E., Dutta, A. J. Biol. Chem. (1996) [Pubmed]
  15. Detection of circulating antigens of Toxoplasma gondii in human infection. Hafid, J., Tran Manh Sung, R., Raberin, H., Akono, Z.Y., Pozzetto, B., Jana, M. Am. J. Trop. Med. Hyg. (1995) [Pubmed]
  16. Morphological, contrast-enhanced and spin labeling perfusion imaging for monitoring of relapse after RF ablation of renal cell carcinomas. Boss, A., Martirosian, P., Schraml, C., Clasen, S., Fenchel, M., Anastasiadis, A., Claussen, C.D., Pereira, P.L., Schick, F. European radiology. (2006) [Pubmed]
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