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Gene Review

RPA4  -  replication protein A4, 30kDa

Homo sapiens

Synonyms: HSU24186, RF-A protein 4, RP-A p30, Replication factor A protein 4, Replication protein A 30 kDa subunit
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Disease relevance of RPA4


Psychiatry related information on RPA4


High impact information on RPA4


Chemical compound and disease context of RPA4


Biological context of RPA4


Anatomical context of RPA4

  • Rpa4 is preferentially expressed in placental and colon mucosa tissues [15].
  • In unirradiated HeLa cells, RPA-p34 is phosphorylated primarily at Ser-23 and Ser-29 [16].
  • We conclude that the assay for p30 offers a more sensitive and specific method of semen detection in rape investigation than the enzyme assay for prostatic acid phosphatase [7].
  • Antigens related to the major structural protein (p30) of type C viruses isolated from a woolly monkey and a gibbon ape were found in peripheral white blood cells from five patients with acute leukemia [17].
  • Detection and immunochemical characterization of a primate type C retrovirus-related p30 protein in normal human placentas [18].

Associations of RPA4 with chemical compounds

  • The additional 32 NH2-terminal residues made p32 a significantly more potent transcriptional activator than p30 [14].
  • The disruption of the p30 gene caused the growth retardation of yeast cells in a glycerol medium but not in a glucose medium, i.e. the impairment of the mitochondrial ATP synthesis [19].
  • C-1027 and neocarzinostatin doses that caused similar levels of DNA damage resulted in equivalent increases in RPA32 hyperphosphorylation and RPA nuclear retention and decreases in replication activity, suggesting a common response to enediyne-induced DNA damage [20].
  • At least four of the eight serines and one threonine in the N-terminal 33 residues of RPA-p34 can become phosphorylated after UV irradiation [16].
  • Peptide maps of 48,000-dalton CNBr fragments of m3 P70 and ml P60 were similar and suggested that both polyproteins were similar through the NH2-terminal two-thirds of p30 [21].

Regulatory relationships of RPA4

  • Further mutational studies indicated that, although the N-terminal 32-amino acid peptide of C/EBPepsilon isoform p32 did not greatly influence the transactivation activity compared with p30 isoform, this peptide does modulate transactivation activity [22].

Other interactions of RPA4

  • Among the nine polypeptides detected by immunoblotting, 38 recognized p14, 17 recognized p8, and 16 recognized p8, and 16 recognized p30 [23].
  • The p30 isoform lacked significant transcriptional activity, suggesting that other hematopoietic-specific factors were required for its function [14].

Analytical, diagnostic and therapeutic context of RPA4


  1. Cells transformed by certain strains of Moloney sarcoma virus contain murine p60. Robey, W.G., Oskarsson, M.K., Woude, G.F., Naso, R.B., Arlinghaus, R.B., Haapala, D.K., Fischinger, P.J. Cell (1977) [Pubmed]
  2. Autoantibodies against the replication protein A complex in systemic lupus erythematosus and other autoimmune diseases. Yamasaki, Y., Narain, S., Hernandez, L., Barker, T., Ikeda, K., Segal, M.S., Richards, H.B., Chan, E.K., Reeves, W.H., Satoh, M. Arthritis Res. Ther. (2006) [Pubmed]
  3. Isolation of type C virions from a normal human fibroblast strain. Panem, S., Prochownik, E.V., Reale, F.R., Kirsten, W.H. Science (1975) [Pubmed]
  4. Structural mechanism of RPA loading on DNA during activation of a simple pre-replication complex. Jiang, X., Klimovich, V., Arunkumar, A.I., Hysinger, E.B., Wang, Y., Ott, R.D., Guler, G.D., Weiner, B., Chazin, W.J., Fanning, E. EMBO J. (2006) [Pubmed]
  5. A sensitive sandwich enzyme immunoassay for gamma-seminoprotein and its application to sex discrimination of blood and bloodstains. Yukawa, N., Kohno, T., Ishikawa, E., Takahama, K. Forensic Sci. Int. (1992) [Pubmed]
  6. Replication protein A: a heterotrimeric, single-stranded DNA-binding protein required for eukaryotic DNA metabolism. Wold, M.S. Annu. Rev. Biochem. (1997) [Pubmed]
  7. Postcoital detection of a male-specific semen protein. Application to the investigation of rape. Graves, H.C., Sensabaugh, G.F., Blake, E.T. N. Engl. J. Med. (1985) [Pubmed]
  8. Immunohistochemical analysis of Ewing's sarcoma cell surface antigen p30/32MIC2. Fellinger, E.J., Garin-Chesa, P., Triche, T.J., Huvos, A.G., Rettig, W.J. Am. J. Pathol. (1991) [Pubmed]
  9. Quantitative immunoelectrophoretic assay for murine oncornavirus p30: noncovalent facilitation by sodium dodecyl sulfate. Robinson, O.R., Shibley, G.P., Sevoian, M. Infect. Immun. (1977) [Pubmed]
  10. In vitro cleavage of Pr65gag by the Moloney murine leukaemia virus proteolytic activity yields p30 whose NH2-terminal sequence is identical to virion p30. Yoshinaka, Y., Shames, R.B., Luftig, R.B., Smythers, G.W., Oroszlan, S. J. Gen. Virol. (1985) [Pubmed]
  11. Virus p30-related protein in follicular fluids and C virus-like particles on the cell membrane of the human oocyte. Nilsson, B.O., Larsson, E., Sundström, P. Journal of in vitro fertilization and embryo transfer : IVF. (1986) [Pubmed]
  12. Preferential localization of hyperphosphorylated replication protein A to double-strand break repair and checkpoint complexes upon DNA damage. Wu, X., Yang, Z., Liu, Y., Zou, Y. Biochem. J. (2005) [Pubmed]
  13. Human replication protein A unfolds telomeric G-quadruplexes. Salas, T.R., Petruseva, I., Lavrik, O., Bourdoncle, A., Mergny, J.L., Favre, A., Saintom??, C. Nucleic Acids Res. (2006) [Pubmed]
  14. C/EBPepsilon directly interacts with the DNA binding domain of c-myb and cooperatively activates transcription of myeloid promoters. Verbeek, W., Gombart, A.F., Chumakov, A.M., Müller, C., Friedman, A.D., Koeffler, H.P. Blood (1999) [Pubmed]
  15. Rpa4, a homolog of the 34-kilodalton subunit of the replication protein A complex. Keshav, K.F., Chen, C., Dutta, A. Mol. Cell. Biol. (1995) [Pubmed]
  16. Sites of UV-induced phosphorylation of the p34 subunit of replication protein A from HeLa cells. Zernik-Kobak, M., Vasunia, K., Connelly, M., Anderson, C.W., Dixon, K. J. Biol. Chem. (1997) [Pubmed]
  17. Primate type C virus p30 antigen in cells from humans with acute leukemia. Sherr, C.J., Todaro, G.J. Science (1975) [Pubmed]
  18. Detection and immunochemical characterization of a primate type C retrovirus-related p30 protein in normal human placentas. Jerabek, L.B., Mellors, R.C., Elkon, K.B., Mellors, J.W. Proc. Natl. Acad. Sci. U.S.A. (1984) [Pubmed]
  19. p32 protein, a splicing factor 2-associated protein, is localized in mitochondrial matrix and is functionally important in maintaining oxidative phosphorylation. Muta, T., Kang, D., Kitajima, S., Fujiwara, T., Hamasaki, N. J. Biol. Chem. (1997) [Pubmed]
  20. The cellular response to DNA damage induced by the enediynes C-1027 and neocarzinostatin includes hyperphosphorylation and increased nuclear retention of replication protein a (RPA) and trans inhibition of DNA replication. McHugh, M.M., Yin, X., Kuo, S.R., Liu, J.S., Melendy, T., Beerman, T.A. Biochemistry (2001) [Pubmed]
  21. Chemical determination of the m1 Moloney sarcoma virus pP60gag gene order: evidence for unique peptides in the carboxy terminus of the polyprotein. Oskarsson, M.K., Elder, J.H., Gautsch, J.W., Lerner, R.A., Vande Woude, G.F. Proc. Natl. Acad. Sci. U.S.A. (1978) [Pubmed]
  22. Structural and functional studies of CCAAT/enhancer-binding protein epsilon. Tang, J.G., Koeffler, H.P. J. Biol. Chem. (2001) [Pubmed]
  23. Detection of circulating antigens of Toxoplasma gondii in human infection. Hafid, J., Tran Manh Sung, R., Raberin, H., Akono, Z.Y., Pozzetto, B., Jana, M. Am. J. Trop. Med. Hyg. (1995) [Pubmed]
  24. Expression of antigenic crossreactivity to RD114 p 30 protein in a human fibrosarcoma cell line. Smith, H.S., Riggs, J.L., Springer, E.L. Proc. Natl. Acad. Sci. U.S.A. (1977) [Pubmed]
  25. Highly efficient induction of type C retroviruses by a human tumor in athymic mice. Gautsch, J.W., Knowles, A.F., Jensen, F.C., Kaplan, N.O. Proc. Natl. Acad. Sci. U.S.A. (1980) [Pubmed]
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