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QTRT1  -  queuine tRNA-ribosyltransferase 1

Homo sapiens

Synonyms: FP3235, Guanine insertion enzyme, Queuine tRNA-ribosyltransferase, TGT, TGUT, ...
 
 
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Disease relevance of QTRT1

  • A search of the human EST database for sequences with significant homology to the well studied TGT from Escherichia coli identified several candidates for full-length (1.3-1.4 kb) cDNA clones [1].
  • The nucleotide sequence of the codon in 53% of ductal hyperplastic foci was TGT or AGT, both of which were not found in 30 cases of adenocarcinoma [2].
  • For this purpose, riboflavin-mediated photosensitization of a TGT oligonucleotide in aerated aqueous solution in the presence of the KKK tripeptide was performed [3].
  • Three parameters of coagulability--thrombin generation time (TGT), antithrombin III (AT III), and activated partial thromboplastin time (ATPP)--and two parameters of diabetic control--serial measurements of fasting serum glucose (FG) and hemoglobin A1(HbA1)--were used to study the relationship between diabetic control and hypercoagulability [4].
  • A point mutation in glycoprotein IX coding sequence (Cys73 (TGT) to Tyr(TAT)) causes impaired surface expression of GPIb/IX/V complex in two families with Bernard-Soulier syndrome [5].
 

High impact information on QTRT1

  • Nucleotide sequence analysis localized a G----T point mutation in the first position of helical codon 43, replacing the expected glycine (GGT) residue with a cysteine (TGT) [6].
  • These three SNPs are in one major haplotype block, with TGT representing 78.4% of alleles [7].
  • The TGT/TGT diplotype found in 62.2% of samples was the major genotype seen to modify eye color, with a frequency of 0.905 in blue or green compared with only 0.095 in brown eye color [7].
  • This genotype was also at highest frequency in subjects with light brown hair and was more frequent in fair and medium skin types, consistent with the TGT haplotype acting as a recessive modifier of lighter pigmentary phenotypes [7].
  • The in-frame TGA codon of the lung Sps2 was genetically altered to TGT (Cys) to obtain the Sps2Cys gene [8].
 

Chemical compound and disease context of QTRT1

  • The small cell lung cancer cell line NCI-H209, previously shown to harbour a point mutation in codon 706: TGT- greater than TTT (Cys- greater than Phe), was detected using CDGE [9].
  • In addition, one of 15 PNETs retained heterozygosity but demonstrated a somatic CGT to TGT transition (arg to cys) at codon 273. p53 mutations were absent in other histologic subtypes and in two cases with multiple primary cancers [10].
  • Sequence analysis of the DNAs extracted from paraffin sections of pituitary, parathyroid, and pancreas tumors demonstrated the substitution of thymidine for cytidine in codon 201 of the Gs alpha gene that resulted in replacement of arginine (CGT) with cysteine (TGT) only in the pituitary adenoma, but not in the parathyroid and pancreas tumors [11].
 

Biological context of QTRT1

 

Anatomical context of QTRT1

  • When this construct was transfected into the TGT-negative cell line, GC(3)/c1 (Gündüz et al., 1992, Biochim. Biophys. Acta 1139, 229-238), it restored the ability of the cells to form Q-containing tRNA [1].
  • Examining fractionated mononuclear cells from bone marrow or peripheral blood, an N-ras mutation at position 13 was observed in one patient with overt leukemia, resulting in a base change from GGT to TGT thus converting glycine to cysteine [14].
  • The base sequence selectivity of DNA alkylation for a series of structurally related 1,3-dialkyl-3-acyltriazenes was examined with calf thymus DNA or polymers containing the sequences GGG, CGC, TGT, and AGA [15].
  • Immunohistochemically, USP14/TGT60 kD was absent or weakly localized in the cytoplasm of normal colorectal epithelial cells [16].
  • USP14/TGT60 kD expression correlated with pathological stage (P=0.03), and lymph node (P=0.03) and liver (P=0.03) metastases [16].
 

Associations of QTRT1 with chemical compounds

  • Eukaryotes synthesize Q by the base-for-base exchange of queuine (Q base) for guanine in the unmodified tRNA, a reaction catalyzed by TGT [1].
  • Selectivity of the leading thiazole was modulated by the head group, with N-terminal formyl TIP exhibiting up to 3-fold specificity for AGT over TGT, suggesting that N-formyl-thiazole may provide sequence discrimination of adenine over thymine [17].
  • The TGT sequence is located on the 5' boundary of the pyrimidine-rich region upstream of the nucleotide 2157 3' splice site [18].
  • Sequencing of AR transcripts revealed that the AR in LNCaP-cxD cells harbors a novel mutation in codon 741, TGG (tryptophan) to TGT (cysteine; W741C), or in codon 741, TGG to TTG (leucine; W741L), in the ligand-binding domain [19].
  • Bacterial tRNA-guanine transglycosylase (TGT) replaces the G in position 34 of tRNA with preQ(1), the precursor to the modified nucleoside queuosine [20].
 

Other interactions of QTRT1

  • Furthermore, the percentage of patients strongly positive for USP14/TGT60 kD expression increased with pathological stage [16].
 

Analytical, diagnostic and therapeutic context of QTRT1

  • The activity of the TGT enzyme was restored after treatment of the cells with the protein kinase C activator, TPA, even in the presence of mRNA or protein synthesis inhibitors [21].
  • In bile, K-ras codon 12 mutations were detected in 16 of 46 malignant cases by real-time PCR with the TGT probe and 15 by RFLP analysis [22].
  • Oligodeoxyribonucleotide duplexes (15-bp) containing the single, site-specific monofunctional adduct at G-residues of the central sequences TGT/ACA or 5'-AGT/5'-ACT were prepared and analyzed by differential scanning calorimetry, temperature-dependent ultraviolet absorption and circular dichroism [23].
  • To determine the frequency of abnormal immunologic tests among women experiencing reproductive failure, 108 patients were evaluated for the presence of antiphospholipid antibodies (APA); lupus anticoagulant (LA); thyroid-thyroglobulin and microsomal antibodies (TGT); embryotoxic factor (ETA); and systemic CD56+/CD16- cells [24].

References

  1. Characterization of cDNA encoding the human tRNA-guanine transglycosylase (TGT) catalytic subunit. Deshpande, K.L., Katze, J.R. Gene (2001) [Pubmed]
  2. Analysis of K-ras gene mutation in hyperplastic duct cells of the pancreas without pancreatic disease. Tada, M., Ohashi, M., Shiratori, Y., Okudaira, T., Komatsu, Y., Kawabe, T., Yoshida, H., Machinami, R., Kishi, K., Omata, M. Gastroenterology (1996) [Pubmed]
  3. Characterization of Lysine-Guanine Cross-Links upon One-Electron Oxidation of a Guanine-Containing Oligonucleotide in the Presence of a Trilysine Peptide. Perrier, S., Hau, J., Gasparutto, D., Cadet, J., Favier, A., Ravanat, J.L. J. Am. Chem. Soc. (2006) [Pubmed]
  4. Plasma antithrombin III and thrombin generation time: correlation with hemoglobin A1 and fasting serum glucose in young diabetic women. Sowers, J.R., Tuck, M.L., Sowers, D.K. Diabetes Care (1980) [Pubmed]
  5. A point mutation in glycoprotein IX coding sequence (Cys73 (TGT) to Tyr(TAT)) causes impaired surface expression of GPIb/IX/V complex in two families with Bernard-Soulier syndrome. Noda, M., Fujimura, K., Takafuta, T., Shimomura, T., Fujii, T., Katsutani, S., Fujimoto, T., Kuramoto, A., Yamazaki, T., Mochizuki, T., Matsuzaki, M., Sano, M. Thromb. Haemost. (1996) [Pubmed]
  6. An osteopenic nonfracture syndrome with features of mild osteogenesis imperfecta associated with the substitution of a cysteine for glycine at triple helix position 43 in the pro alpha 1(I) chain of type I collagen. Shapiro, J.R., Stover, M.L., Burn, V.E., McKinstry, M.B., Burshell, A.L., Chipman, S.D., Rowe, D.W. J. Clin. Invest. (1992) [Pubmed]
  7. A Three-Single-Nucleotide Polymorphism Haplotype in Intron 1 of OCA2 Explains Most Human Eye-Color Variation. Duffy, D.L., Montgomery, G.W., Chen, W., Zhao, Z.Z., Le, L., James, M.R., Hayward, N.K., Martin, N.G., Sturm, R.A. Am. J. Hum. Genet. (2007) [Pubmed]
  8. Selenophosphate synthetase genes from lung adenocarcinoma cells: Sps1 for recycling L-selenocysteine and Sps2 for selenite assimilation. Tamura, T., Yamamoto, S., Takahata, M., Sakaguchi, H., Tanaka, H., Stadtman, T.C., Inagaki, K. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  9. No alterations in exon 21 of the RB1 gene in sarcomas and carcinomas of the breast, colon, and lung. Hovig, E., Smith-Sørensen, B., Gebhardt, M.C., Ryberg, D., Lothe, R., Børresen, A.L. Genes Chromosomes Cancer (1992) [Pubmed]
  10. p53 gene mutations in pediatric brain tumors. Felix, C.A., Slavc, I., Dunn, M., Strauss, E.A., Phillips, P.C., Rorke, L.B., Sutton, L., Bunin, G.R., Biegel, J.A. Med. Pediatr. Oncol. (1995) [Pubmed]
  11. A pituitary specific point mutation of codon 201 of the Gs alpha gene in a pituitary adenoma of a patient with multiple endocrine neoplasia (MEN) type 1. Hosoi, E., Yokogoshi, Y., Hosoi, E., Yokoi, K., Sano, T., Saito, S. Endocrinol. Jpn. (1992) [Pubmed]
  12. Characterization of the molecular basis of the alpha 1-antitrypsin F allele. Okayama, H., Brantly, M., Holmes, M., Crystal, R.G. Am. J. Hum. Genet. (1991) [Pubmed]
  13. AML1A and AML1B can transactivate the human IL-3 promoter. Uchida, H., Zhang, J., Nimer, S.D. J. Immunol. (1997) [Pubmed]
  14. Point mutation of the ras protooncogenes and chromosome aberrations in acute nonlymphocytic leukemia and preleukemia related to therapy with alkylating agents. Pedersen-Bjergaard, J., Janssen, W.G., Lyons, J., Philip, P., Bartram, C.R. Cancer Res. (1988) [Pubmed]
  15. Base sequence selectivity in the alkylation of DNA by 1,3-dialkyl-3-acyltriazenes. Kroeger Smith, M.B., Taneyhill, L.A., Michejda, C.J., Smith, R.H. Chem. Res. Toxicol. (1996) [Pubmed]
  16. Ubiquitin-specific protease 14 expression in colorectal cancer is associated with liver and lymph node metastases. Shinji, S., Naito, Z., Ishiwata, S., Ishiwata, T., Tanaka, N., Furukawa, K., Suzuki, H., Seya, T., Matsuda, A., Katsuta, M., Tajiri, T. Oncol. Rep. (2006) [Pubmed]
  17. DNA sequence recognition in the minor groove by crosslinked polyamides: The effect of N-terminal head group and linker length on binding affinity and specificity. O'Hare, C.C., Mack, D., Tandon, M., Sharma, S.K., Lown, J.W., Kopka, M.L., Dickerson, R.E., Hartley, J.A. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  18. Virus deletion mutants that affect a 3' splice site in the E3 transcription unit of adenovirus 2. Bhat, B.M., Brady, H.A., Wold, W.S. Mol. Cell. Biol. (1985) [Pubmed]
  19. Novel mutations of androgen receptor: a possible mechanism of bicalutamide withdrawal syndrome. Hara, T., Miyazaki, J., Araki, H., Yamaoka, M., Kanzaki, N., Kusaka, M., Miyamoto, M. Cancer Res. (2003) [Pubmed]
  20. The RNA-binding PUA domain of archaeal tRNA-guanine transglycosylase is not required for archaeosine formation. Sabina, J., Söll, D. J. Biol. Chem. (2006) [Pubmed]
  21. Involvement of protein kinase C in the control of tRNA modification with queuine in HeLa cells. Langgut, W., Reisser, T. Nucleic Acids Res. (1995) [Pubmed]
  22. Rapid detection of K-ras mutations in bile by peptide nucleic acid-mediated PCR clamping and melting curve analysis: comparison with restriction fragment length polymorphism analysis. Chen, C.Y., Shiesh, S.C., Wu, S.J. Clin. Chem. (2004) [Pubmed]
  23. Effects of monofunctional adducts of platinum(II) complexes on thermodynamic stability and energetics of DNA duplexes. Bursova, V., Kasparkova, J., Hofr, C., Brabec, V. Biophys. J. (2005) [Pubmed]
  24. Laboratory evaluation of women experiencing reproductive failure. Roussev, R.G., Kaider, B.D., Price, D.E., Coulam, C.B. Am. J. Reprod. Immunol. (1996) [Pubmed]
 
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