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Ugt2b15  -  UDP glucuronosyltransferase 2 family,...

Rattus norvegicus

Synonyms: UDP-glucuronosyltransferase 2B15, UDP-glucuronosyltransferase 2B36, UDPGT 2B15, UDPGT 2B36, Ugt2b12, ...
 
 
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Disease relevance of Ugt2b4

 

High impact information on Ugt2b4

  • UDPGT isoform I (elution pH 8.7) from Wistar (RHA) and Gunn rats was active toward 4-nitrophenol [1].
  • Isoforms II (elution pH 8.4), III (elution pH 8.0), and IV (elution pH 7.8) from Gunn rats had normal UDPGT activities, except that Isoform IV was inactive toward bilirubin [1].
  • The corresponding isoform from Gunn rat liver was enzymically inactive but exhibited normal elution pH and mobility on NaDodSO4/polyacrylamide gel electrophoresis (Mr 53,000), and was recognized by a UDPGT-specific antiserum [1].
  • The isoform from Gunn rat liver had only 10% of normal UDPGT activity, however UDPGT activity increased to normal upon addition of 15 mM diethylnitrosamine in vitro [1].
  • Of the purified enzymes investigated, only 3 alpha-hydroxysteroid UDPGT catalyzed the glucuronidation of 4-aminobiphenyl. alpha-Naphthylamine and beta-naphthylamine conjugations were catalyzed by 3 alpha-hydroxysteroid, 17 beta-hydroxysteroid, and 3-methylcholanthrene-inducible p-nitrophenol UDPGTs [4].
 

Biological context of Ugt2b4

 

Anatomical context of Ugt2b4

  • This work describes the purification and characterization of a 4-HBP UDPGT from rat liver microsomes and, furthermore, provides evidence that suggests that this UDPGT is different from another UDPGT previously shown to react with 4-HBP and chloramphenicol [10].
  • Immunoblot analysis of microsomes from developing rat liver demonstrated that the deficiency in bilirubin and testosterone glucuronidation in the fetus was due to the absence of the UDPGT isoenzyme proteins responsible for these conjugations [11].
  • Expression driven by the SV40 promoter produced enzymatically active UDPGT in COS-7 cells cultured in vitro [12].
  • CONCLUSION: Transplants of small intestine with known UDPGT activity partially corrected the deficiency in Gunn rats and allayed the hyperbilirubinemia [2].
  • Steroid glucuronidation was investigated in solubilized female rat and rabbit liver microsomes and in preparations of UDP-glucuronsyltransferase (UDPGT) activity resolved from these organelles [13].
 

Associations of Ugt2b4 with chemical compounds

 

Analytical, diagnostic and therapeutic context of Ugt2b4

  • The UDPGT isoenzymes were purified utilizing Chromatofocusing, column isoelectric focusing, and UDP-hexanolamine Sepharose 4B affinity chromatography [15].
  • The activity of hepatic glutathione S-transferase (GST) and UDP-glucuronyl transferase (UDPGT) and the level of glutathione (GSH) were estimated at 0, 45 and 90 days of treatment as well as 28 days after cessation of treatment [16].
  • The Michaelis-Menten kinetic parameters (Km and Vmax) associated with the formation of 4Me7OHC and APAP glucuronides demonstrated that the immobilization had not significantly affected the enzymatic activity of the UDPGT relative to the non-immobilized enzyme [17].

References

  1. Isolation of multiple normal and functionally defective forms of uridine diphosphate-glucuronosyltransferase from inbred Gunn rats. Roy Chowdhury, N., Gross, F., Moscioni, A.D., Kram, M., Arias, I.M., Roy Chowdhury, J. J. Clin. Invest. (1987) [Pubmed]
  2. Correction of congenital indirect hyperbilirubinemia by small intestinal transplantation. Medley, M.M., Hooker, R.L., Rabinowitz, S., Holton, R., Jaffe, B.M. Am. J. Surg. (1995) [Pubmed]
  3. Thyroid hypertrophic effect of semotiadil fumarate, a new calcium antagonist, in rats. Tsuchiya, Y., Ohno, H., Satoh, H., Sugawara, T., Kajimura, T., Nomura, M. The Journal of toxicological sciences. (2000) [Pubmed]
  4. N-glucuronidation of carcinogenic aromatic amines catalyzed by rat hepatic microsomal preparations and purified rat liver uridine 5'-diphosphate-glucuronosyltransferases. Green, M.D., Tephly, T.R. Cancer Res. (1987) [Pubmed]
  5. Phenobarbital induction of CYP2B1/2 in primary hepatocytes: endocrine regulation and evidence for a single pathway for multiple inducers. Ganem, L.G., Trottier, E., Anderson, A., Jefcoate, C.R. Toxicol. Appl. Pharmacol. (1999) [Pubmed]
  6. Thyroxine and 3,3',5-triiodothyronine are glucuronidated in rat liver by different uridine diphosphate-glucuronyltransferases. Beetstra, J.B., van Engelen, J.G., Karels, P., van der Hoek, H.J., de Jong, M., Docter, R., Krenning, E.P., Hennemann, G., Brouwer, A., Visser, T.J. Endocrinology (1991) [Pubmed]
  7. Glucuronidation of 3'-azido-3'-deoxythymidine: human and rat enzyme specificity. Resetar, A., Spector, T. Biochem. Pharmacol. (1989) [Pubmed]
  8. UDP glucuronosyltransferase gene expression is involved in the stimulation of ascorbic acid biosynthesis by xenobiotics in rats. Horio, F., Shibata, T., Makino, S., Machino, S., Hayashi, Y., Hattori, T., Yoshida, A. J. Nutr. (1993) [Pubmed]
  9. Developmental changes in hepatic microsomal interactions between UDP-glucuronyltransferase and phospholipid in the rat fetus. Nanbo, T. Reprod. Toxicol. (1998) [Pubmed]
  10. Purification and properties of a rat liver phenobarbital-inducible 4-hydroxybiphenyl UDP-glucuronosyltransferase. Styczynski, P., Green, M., Puig, J., Coffman, B., Tephly, T. Mol. Pharmacol. (1991) [Pubmed]
  11. The inadequacy of perinatal glucuronidation: immunoblot analysis of the developmental expression of individual UDP-glucuronosyltransferase isoenzymes in rat and human liver microsomes. Coughtrie, M.W., Burchell, B., Leakey, J.E., Hume, R. Mol. Pharmacol. (1988) [Pubmed]
  12. Examination of the substrate specificity of cloned rat kidney phenol UDP-glucuronyltransferase expressed in COS-7 cells. Jackson, M.R., Fournel-Gigleux, S., Harding, D., Burchell, B. Mol. Pharmacol. (1988) [Pubmed]
  13. Steroid 3- and 17-OH UDP-glucuronosyltransferase activities in rat and rabbit liver microsomes. Falany, C.N., Chowdhury, J.R., Chowdhury, N.R., Tephly, T.R. Drug Metab. Dispos. (1983) [Pubmed]
  14. Endocrine factors modulate the phenobarbital-mediated induction of cytochromes P450 and phase II enzymes in a similar strain-dependent manner. Ganem, L.G., Jefcoate, C.R. Toxicol. Appl. Pharmacol. (1998) [Pubmed]
  15. Separation, purification and characterization of three isoenzymes of UDP-glucuronyltransferase from rat liver microsomes. Falany, C.N., Tephly, T.R. Arch. Biochem. Biophys. (1983) [Pubmed]
  16. Alterations in hepatic detoxifying enzymes induced by new organophosphorus insecticides following subchronic exposure in rats. Mahboob, M., Siddiqui, M.K. Journal of applied toxicology : JAT. (2001) [Pubmed]
  17. The covalent immobilization of microsomal uridine diphospho-glucuronosyltransferase (UDPGT): initial synthesis and characterization of an UDPGT immobilized enzyme reactor for the on-line study of glucuronidation. Kim, H.S., Wainer, I.W. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2005) [Pubmed]
 
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