Gene Review:
folA - dihydrofolate reductase
Escherichia coli str. K-12 substr. MG1655
Synonyms:
ECK0049, JW0047, tmrA
- Conditional dihydrofolate reductase deficiency due to transposon Tn5tac1 insertion downstream from the folA gene in Escherichia coli. Neuwald, A.F., Krishnan, B.R., Ahrweiler, P.M., Frieden, C., Berg, D.E. Gene (1993)
- Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 A resolution. II. Environment of bound NADPH and implications for catalysis. Filman, D.J., Bolin, J.T., Matthews, D.A., Kraut, J. J. Biol. Chem. (1982)
- Production of cyclic peptides and proteins in vivo. Scott, C.P., Abel-Santos, E., Wall, M., Wahnon, D.C., Benkovic, S.J. Proc. Natl. Acad. Sci. U.S.A. (1999)
- Species-specific irreversible inhibition of Neisseria gonorrhoeae dihydrofolate reductase by a substituted 2,4-diamino-5-benzylpyrimidine. Tansik, R.L., Averett, D.R., Roth, B., Paterson, S.J., Stone, D., Baccanari, D.P. J. Biol. Chem. (1984)
- The structure of the mutant dihydrofolate reductase from Streptococcus faecium. Amino acid sequence of peptide CNBr 7 and complete sequence of the protein. Peterson, D.L., Gleisner, J.M., Blakley, R.L. J. Biol. Chem. (1975)
- Insights into enzyme function from studies on mutants of dihydrofolate reductase. Benkovic, S.J., Fierke, C.A., Naylor, A.M. Science (1988)
- Neutron diffraction studies of Escherichia coli dihydrofolate reductase complexed with methotrexate. Bennett, B., Langan, P., Coates, L., Mustyakimov, M., Schoenborn, B., Howell, E.E., Dealwis, C. Proc. Natl. Acad. Sci. U.S.A. (2006)
- Impact of distal mutations on the network of coupled motions correlated to hydride transfer in dihydrofolate reductase. Wong, K.F., Selzer, T., Benkovic, S.J., Hammes-Schiffer, S. Proc. Natl. Acad. Sci. U.S.A. (2005)
- Single-molecule and transient kinetics investigation of the interaction of dihydrofolate reductase with NADPH and dihydrofolate. Zhang, Z., Rajagopalan, P.T., Selzer, T., Benkovic, S.J., Hammes, G.G. Proc. Natl. Acad. Sci. U.S.A. (2004)
- Binding sites in Escherichia coli dihydrofolate reductase communicate by modulating the conformational ensemble. Pan, H., Lee, J.C., Hilser, V.J. Proc. Natl. Acad. Sci. U.S.A. (2000)
- Use of the Escherichia coli chromosomal DHFR gene as selection marker in mammalian cells. Asselbergs, F.A., Widmer, R. J. Biotechnol. (1995)
- Dihydrofolate reductase: the amino acid sequence of the enzyme from a methotrexate-resistant mutant of Escherichia coli. Bennett, C.D., Rodkey, J.A., Sondey, J.M., Hirschmann, R. Biochemistry (1978)
- Crystal structures of Escherichia coli dihydrofolate reductase: the NADP+ holoenzyme and the folate.NADP+ ternary complex. Substrate binding and a model for the transition state. Bystroff, C., Oatley, S.J., Kraut, J. Biochemistry (1990)
- Crystal structure of unliganded Escherichia coli dihydrofolate reductase. Ligand-induced conformational changes and cooperativity in binding. Bystroff, C., Kraut, J. Biochemistry (1991)
- Effect of a single amino acid substitution on Escherichia coli dihydrofolate reductase catalysis and ligand binding. Baccanari, D.P., Stone, D., Kuyper, L. J. Biol. Chem. (1981)
- Conformation coupled enzyme catalysis: single-molecule and transient kinetics investigation of dihydrofolate reductase. Antikainen, N.M., Smiley, R.D., Benkovic, S.J., Hammes, G.G. Biochemistry (2005)
- Regulation of dihydrofolate reductase synthesis in Escherichia coli. Smith, D.R., Calvo, J.M. Mol. Gen. Genet. (1979)
- Structural requirements for transport of preprocecropinA and related presecretory proteins into mammalian microsomes. Schlenstedt, G., Gudmundsson, G.H., Boman, H.G., Zimmermann, R. J. Biol. Chem. (1992)
- Transport of the yeast ATP synthase beta-subunit into mitochondria. Effects of amino acid substitutions on targeting. Walker, M.E., Valentin, E., Reid, G.A. Biochem. J. (1990)
- Novel fluorinated antifolates. Enzyme inhibition and cytotoxicity studies on 2'- and 3'-fluoroaminopterin. Henkin, J., Washtien, W.L. J. Med. Chem. (1983)
- Viral Q beta RNA as a high expression vector for mRNA translation in a cell-free system. Katanaev, V.L., Kurnasov, O.V., Spirin, A.S. FEBS Lett. (1995)
- Quantitation of 2-amino-3-methylimidazo[4,5-f]quinoline and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline DNA adducts in specific sequences using alkali or uvrABC excinuclease. Nouso, K., Bohr, V.A., Schut, H.A., Snyderwine, E.G. Mol. Carcinog. (1993)
- CsgD, a regulator of curli and cellulose synthesis, also regulates serine hydroxymethyltransferase synthesis in Escherichia coli K-12. Chirwa, N.T., Herrington, M.B. Microbiology (Reading, Engl.) (2003)
- Growth properties of a folA null mutant of Escherichia coli K12. Herrington, M.B., Chirwa, N.T. Can. J. Microbiol. (1999)
- A mutation in the folA promoter delays adaptation to minimal medium by Escherichia coli K-12. Herrington, M.B., MacRae, T.J., Panagopoulos, D., Wong, S.H. J. Basic Microbiol. (2002)
- Role of Lys-32 residues in R67 dihydrofolate reductase probed by asymmetric mutations. Hicks, S.N., Smiley, R.D., Stinnett, L.G., Minor, K.H., Howell, E.E. J. Biol. Chem. (2004)
- "Catch 222," the effects of symmetry on ligand binding and catalysis in R67 dihydrofolate reductase as determined by mutations at Tyr-69. Stinnett, L.G., Smiley, R.D., Hicks, S.N., Howell, E.E. J. Biol. Chem. (2004)
- Nucleotide sequence of the thymidylate synthase B and dihydrofolate reductase genes contained in one Bacillus subtilis operon. Iwakura, M., Kawata, M., Tsuda, K., Tanaka, T. Gene (1988)
- The dihydrofolate reductase domain of Plasmodium falciparum thymidylate synthase-dihydrofolate reductase. Gene synthesis, expression, and anti-folate-resistant mutants. Sirawaraporn, W., Prapunwattana, P., Sirawaraporn, R., Yuthavong, Y., Santi, D.V. J. Biol. Chem. (1993)
- Identification of an uncleavable targeting signal in the 70-kilodalton spinach chloroplast outer envelope membrane protein. Wu, C., Ko, K. J. Biol. Chem. (1993)
- Dihydrofolate reductase from amethopterin-resistant Lactobacillus casei. Sequences of the cyanogen bromide peptides and complete sequences of the enzyme. Freisheim, J.H., Bitar, K.G., Reddy, A.V., Blankenship, D.T. J. Biol. Chem. (1978)
- Testing the relationship between foldability and the early folding events of dihydrofolate reductase from Escherichia coli. Arai, M., Maki, K., Takahashi, H., Iwakura, M. J. Mol. Biol. (2003)