The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

flgB  -  flagellar component of cell-proximal...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1058, JW1060, flbA
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of flgB

  • Genetic and transcriptional analysis of flgB flagellar operon constituents in the oral spirochete Treponema denticola and their heterologous expression in enteric bacteria [1].
  • The different ospC and fla gene products were immunoreactive with monoclonal antibodies and human sera and, thus, enlarge the spectrum of recombinant antigens to improve antibody detection in patients with Lyme borreliosis [2].
  • Genetic analysis of three additional fla genes in Salmonella typhimurium [3].
  • The sequential transcription of flagellar (fla) genes in the Caulobacter crescentus cell cycle is controlled by the organization of these genes in a regulatory hierarchy of four levels (I-IV) [4].
  • We describe here the cloning and expression in Escherichia coli of a cDNA fragment encoding the two putative zinc fingers (FI and FII) domain of the human poly(ADP-ribose) polymerase [5].

High impact information on flgB

  • The deletion strain lacks motility and belongs to the fla(-) mutant class, indicating that it is deficient in flagellar biogenesis [6].
  • The fla gene cluster is involved in the biogenesis of flagella in Halobacterium salinarum [6].
  • We speculate that the conserved sequence elements mapping at -13, -24 and -100 are cis-acting regulatory elements required for the transcription and periodic regulation of these fla genes in the C. crescentus cell cycle [7].
  • Replicon typing of plasmids carrying bla(CTX-M) or bla(CMY) beta-lactamase genes indicates a predominance of I1 and A/C replicons among bla(CMY)-carrying plasmids and five different plasmid scaffolds associated with the different types of bla(CTX-M) genes (I1, FII, HI2, K, and N) [8].
  • Using the previously described pBSV2 as a backbone, a shuttle vector, termed pKFSS1, which carries the aadA open reading frame fused to the B. burgdorferi flgB promoter was constructed [9].

Chemical compound and disease context of flgB

  • The flbA gene decreased nixA transcription. flbA also decreased urease activity three-fold in E. coli containing the P. mirabilis urease locus in a urea- and UreR-dependent fashion [10].
  • I alpha plasmids conferring Cm resistance alone or FII plasmids conferring Cm or Km markers were common in the E. coli strains [11].

Biological context of flgB

  • The FlbT phenotype is unique, since all other identified C. crescentus fla mutations cause a reduction in the levels of the 25- and 27-kDa flagellins [12].
  • An isogenic strain with a mutation in the flbA gene was constructed by disruption of the gene with a kanamycin resistance cassette and electroporation-mediated allelic exchange mutagenesis [13].
  • Disruption of the metal binding ability of the second zinc finger, FII, dramatically reduced target DNA binding [5].
  • Pseudomonas aeruginosa strain GT424, derived by the transfer of plasmid R45 to strain GT1, was shown to have an Era+ phenotype (enhanced recipient ability) for enterobacterial plasmids from incompatibility (Inc) groups C, FI, FII, J, N, W, and X [14].
  • The heat stable and heat labile plasmids also share a significant degree of their polynucleotide sequences with plasmids of the FI and FII incompatibility groups, but not with R factors belonging to the I, N, W, P, or X incompatibility groups [15].

Anatomical context of flgB

  • Methotrexate-mediated amplification of the foreign gene in CHO cells resulted in a more than 10-fold increase in FII expression, while in the presence of methotrexate, 293 cells expressed 200-250 mU/10(6) cells per 24 h [16].
  • Properties of an E. coli O26 mutant with modified cell wall and improved efficiency as recipient in conjugation for an FII plasmid [17].
  • No analogs to the bacterial chemotactic and motility (che, mot, fla) genes, genes for a two-component signal transduction system, genes associated with gliding, or genomic homologs for the eukaryotic cytoskeleton and motor proteins were found in the Mollicutes [18].

Associations of flgB with chemical compounds

  • R6-5 is a low copy number, conjugative, FII incompatibility group plasmid that has a molecular length of 102 kb and that specifies resistance against several antibiotics (chloramphenicol, fusidic acid, kanamycin, streptomycin and sulphonamide) and mercury salts [19].
  • It was found that transcription of genes responsible for the formation and/or function of flagella (hag, fla, mot, che) decreased significantly at 40 degrees C. However, in the ftsI730 mutant at the nonpermissive temperature, or in penicillin G treated wild-type cells, cell division was blocked but formation of flagella continued [20].
  • Two of three R plasmids conferring resistance to Cm, Tc, Sm and Su were classified into Inc B and one to Inc FII [21].

Other interactions of flgB

  • Furthermore, the 22-kDa flagellin was no longer detected in flbA pseudorevertants that assembled functional filaments [12].

Analytical, diagnostic and therapeutic context of flgB

  • Furthermore, there was significantly reduced synthesis of the urease structural subunits in E. coli (pHP8080) containing the flbA gene, as determined by Western blot analysis with UreA and UreB antiserum [22].


  1. Genetic and transcriptional analysis of flgB flagellar operon constituents in the oral spirochete Treponema denticola and their heterologous expression in enteric bacteria. Heinzerling, H.F., Olivares, M., Burne, R.A. Infect. Immun. (1997) [Pubmed]
  2. Genetic heterogenity of the genes coding for the outer surface protein C (OspC) and the flagellin of Borrelia burgdorferi. Jauris-Heipke, S., Fuchs, R., Motz, M., Preac-Mursic, V., Schwab, E., Soutschek, E., Will, G., Wilske, B. Med. Microbiol. Immunol. (Berl.) (1993) [Pubmed]
  3. Genetic analysis of three additional fla genes in Salmonella typhimurium. Yamaguchi, S., Fujita, H., Taira, T., Kutsukake, K., Homma, M., Iino, T. J. Gen. Microbiol. (1984) [Pubmed]
  4. Regulation of the Caulobacter crescentus rpoN gene and function of the purified sigma 54 in flagellar gene transcription. Anderson, D.K., Ohta, N., Wu, J., Newton, A. Mol. Gen. Genet. (1995) [Pubmed]
  5. The second zinc-finger domain of poly(ADP-ribose) polymerase determines specificity for single-stranded breaks in DNA. Gradwohl, G., Ménissier de Murcia, J.M., Molinete, M., Simonin, F., Koken, M., Hoeijmakers, J.H., de Murcia, G. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  6. The fla gene cluster is involved in the biogenesis of flagella in Halobacterium salinarum. Patenge, N., Berendes, A., Engelhardt, H., Schuster, S.C., Oesterhelt, D. Mol. Microbiol. (2001) [Pubmed]
  7. A set of positively regulated flagellar gene promoters in Caulobacter crescentus with sequence homology to the nif gene promoters of Klebsiella pneumoniae. Mullin, D., Minnich, S., Chen, L.S., Newton, A. J. Mol. Biol. (1987) [Pubmed]
  8. Replicon typing of plasmids carrying CTX-M or CMY beta-lactamases circulating among Salmonella and Escherichia coli isolates. Hopkins, K.L., Liebana, E., Villa, L., Batchelor, M., Threlfall, E.J., Carattoli, A. Antimicrob. Agents Chemother. (2006) [Pubmed]
  9. aadA confers streptomycin resistance in Borrelia burgdorferi. Frank, K.L., Bundle, S.F., Kresge, M.E., Eggers, C.H., Samuels, D.S. J. Bacteriol. (2003) [Pubmed]
  10. The Helicobacter pylori flbA flagellar biosynthesis and regulatory gene is required for motility and virulence and modulates urease of H. pylori and Proteus mirabilis. McGee, D.J., Coker, C., Testerman, T.L., Harro, J.M., Gibson, S.V., Mobley, H.L. J. Med. Microbiol. (2002) [Pubmed]
  11. Genetical relationship between R plasmids derived from Salmonella and Escherichia coli obtained from a pig farm, and its epidemiological significance. Ishiguro, N., Goto, J., Sato, G. The Journal of hygiene. (1980) [Pubmed]
  12. Characterization of strains containing mutations in the contiguous flaF, flbT, or flbA-flaG transcription unit and identification of a novel fla phenotype in Caulobacter crescentus. Schoenlein, P.V., Ely, B. J. Bacteriol. (1989) [Pubmed]
  13. Cloning and characterization of the Helicobacter pylori flbA gene, which codes for a membrane protein involved in coordinated expression of flagellar genes. Schmitz, A., Josenhans, C., Suerbaum, S. J. Bacteriol. (1997) [Pubmed]
  14. Transfer of plasmids from Escherichia coli to Pseudomonas aeruginosa: characterization of a Pseudomonas aeruginosa mutant with enhanced recipient ability for enterobacterial plasmids. Tardif, G., Grant, R.B. Antimicrob. Agents Chemother. (1983) [Pubmed]
  15. Polynucleotide sequence relationships among Ent plasmids and the relationship between Ent and other plasmids. So, M., Crosa, J.H., Falkow, S. J. Bacteriol. (1975) [Pubmed]
  16. Overexpression of human prothrombin in permanent cell lines using a dominant selection/amplification fusion marker. Herlitschka, S.E., Falkner, F.G., Schlokat, U., Dorner, F. Protein Expr. Purif. (1996) [Pubmed]
  17. Properties of an E. coli O26 mutant with modified cell wall and improved efficiency as recipient in conjugation for an FII plasmid. Molina, A.M., Coratza, G., Margollicci, M.A., Marri, L., Musmanno, R.A. Microbiologica (1984) [Pubmed]
  18. Shaping and moving a spiroplasma. Trachtenberg, S. J. Mol. Microbiol. Biotechnol. (2004) [Pubmed]
  19. Plasmid replication functions. II. Cloning analysis of the repA replication region of antibiotic resistance plasmid R6-5. Andrés, I., Slocombe, P.M., Cabello, F., Timmis, J.K., Lurz, R., Burkardt, H.J., Timmis, K.N. Mol. Gen. Genet. (1979) [Pubmed]
  20. A cell division regulatory mechanism controls the flagellar regulon in Escherichia coli. Nishimura, A., Hirota, Y. Mol. Gen. Genet. (1989) [Pubmed]
  21. R plasmids conferring multiple drug resistance from shigella isolated in Korea. Chun, D., Cho, D.T., Seol, S.Y., Suh, M.H., Lee, Y.C. The Journal of hygiene. (1984) [Pubmed]
  22. Isolation of Helicobacter pylori genes that modulate urease activity. McGee, D.J., May, C.A., Garner, R.M., Himpsl, J.M., Mobley, H.L. J. Bacteriol. (1999) [Pubmed]
WikiGenes - Universities