Disease relevance of grpE

• The products of the Escherichia coli dnaK, dnaJ, and grpE heat shock genes have been previously shown to be essential for bacteriophage lambda DNA replication at all temperatures and for bacterial survival under certain conditions [1].
• Second, CsaA suppressed the growth defects of dnaK, dnaJ and grpE mutants of E. coli [2].
• A transducing lambda phage, lambdagrpE22, carrying the wild type allele of the grpE gene was constructed in vitro [3].
• We have cloned the Francisella tularensis (Ft) grpE-dnaK-dnaJ heat-shock genes which are organized in that order [4].
• The nucleotide sequence of the dnaK operon cloned from Porphyromonas gingivalis revealed that the operon does not contain homologues of either dnaJ or grpE [5].

High impact information on grpE

• Strains carrying defective dnaK, dnaJ, or grpE alleles have enhanced synthesis of heat shock proteins at low temperature and fail to shut off the heat shock response after shift to high temperature [6].
• We have isolated various missense mutations in the essential grpE gene of Escherichia coli based on the inability to propagate bacteriophage lambda [7].
• Interaction requires Mg ions, whereas MgATP inhibits, and inhibition is stronger in the presence of co-chaperonin GroES. "Heat-shock" of the membrane at 45 degrees C destroys irreversibly its ability to bind GroEL [8].
• Heat shock response in Escherichia coli influences cell division [9].
• Heat shock regulatory gene (htpR) of Escherichia coli is required for growth at high temperature but is dispensable at low temperature [10].

Chemical compound and disease context of grpE

• The effects of temperature, growth stage, and inducer (ethanol) concentration on the kinetics and magnitude of the stress response were investigated by using an Escherichia coli strain with the grpE heat shock promoter fused to the Vibrio fischeri lux genes [11].

Biological context of grpE

• In complete media, the growth of the lambda pi A66 phage (capable of replicating in E. coli dnaJ, dnaK, and grpE missense mutants at 30 degrees C), as well as efficiency of transformation by the lambda pi A66 plasmid, is significantly decreased in a dnaJ259 cbpA::kan double mutant [12].
• Additional heat-inducible transcription start sites were located 69 bases upstream of orfA and 87 bases upstream of grpE; the corresponding promoter regions showed less similarity to other known promoter sequences [13].
• The downstream ORFs showed high homology to the grpE and dnaK genes of other prokaryotes [14].
• A puromycin-resistance cassette (pac cassette) was inserted in the EcoRI site of Int alpha already inserted in pUC18, to obtain a vector which integrated the pac cassette in the chromosome between grpE and dnaK [15].
• Heat shock proteins and RNA polymerase sigma factor play an important role in protecting cells against environmental stresses, including starvation, osmotic and oxidative stresses, and cold shock [16].

Anatomical context of grpE

• Protein misfolding and inclusion body formation in recombinant Escherichia coli cells overexpressing Heat-shock proteins [17].
• Heat shock induction by a misassembled cytoplasmic membrane protein complex in Escherichia coli [18].

Associations of grpE with chemical compounds

• The effect of ATP on the dnaK-grpE complex was also observed during sedimentation of the two proteins in glycerol gradients [19].
• Heat shock also activated this gene while kinetin, ABA and NaCl treatment did not result in MsCPK3 mRNA accumulation [20].

Other interactions of grpE

• A transducing lambda phage carrying grpE, a bacterial gene necessary for lambda DNA replication, and two ribosomal protein genes, rpsP (S16) and rplS (L19) [3].
• The essential Escherichia coli msgB gene, a multicopy suppressor of a temperature-sensitive allele of the heat shock gene grpE, is identical to dapE [21].
• Effects of environmental stresses on the activities of the uspA, grpE and rpoS promoters of Escherichia coli O157:H7 [16].
• Cryotolerance induced by starvation of TSB- and LB-grown E. coli was correlated with the expression of genes involved in general stress response pathways, such as uspA, grpE, and rpoS [22].
• Heat shock regulation in the ftsH null mutant of Escherichia coli: dissection of stability and activity control mechanisms of sigma32 in vivo [23].

Analytical, diagnostic and therapeutic context of grpE

• Sequence analysis and transcriptional regulation of the Escherichia coli grpE gene, encoding a heat shock protein [24].
• Northern blot analyses with grpE-, dnaK-, and dnaJ-specific probes revealed that the three genes are co-transcribed as a 4.0-kb mRNA [25].
• Heat shock proteins and inflammatory acne vulgaris: molecular cloning, overexpression and purification of a propionibacterium acnes GroEL and DnaK homologue [26].
• Heat shock and osmotically dependent steps by DNA uptake after Escherichia coli electroporation [27].

References