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Gene Review

thiL  -  thiamine monophosphate kinase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0411, JW0407
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Disease relevance of thiL

  • RNase P preparations from Escherichia coli can be separated into RNA and protein by chromatography, in buffers containing 7 M urea, on Sephadex G-200, DEAE-Sephadex, or CM-Sephadex columns [1].
  • This NAD glycohydrolase activity co-chromatographed on Sephacryl G-200 in 6.5 M urea, pH 3.2, 0.1 M glycine with the ADP-ribosyltransferase activity of the toxin, as monitored by the transfer of [32P]ADP-ribose from [32P]NAD to a 41,000-Da protein in NG108-15 neuroblastoma X glioma hybrid cells [2].
  • A nucleic acid methylase, N2-guanine ribonucleic acid (RNA) methyltransferase, which is associated with type C RNA tumor viruses, has been purified from avian myeloblastosis virions by gel filtration on Sephadex G-200, followed by chromatography on hydroxylapatite [3].
  • An extracellular bactericidal substance was isolated from the supernatant of Streptococcus mutans Rm-10 culture fluid and partially purified with 60% ammonium sulfate precipitation, differential centrifugation, and gel filtration on Sephadex G-200 [4].
  • We have succeeded in the heterologous expression and the complete and uniform isotopic 15N-labelling of the cytochrome c553 from Desulfovibrio vulgaris Hildenborough, in a sulphate-reducing bacterium, D. desulfuricans G200, by using a growth medium combining 15N-ammonium chloride and 15N-Celtone [5].

High impact information on thiL


Biological context of thiL

  • Deletion of 0.4 kb upstream from orf1 abolished the expression of Hmc in D. desulfuricans G200, indicating this region to contain the hmc operon promoter [11].
  • The non-immunoglobulin component of human milk responsible for the inhibition of Escherichia coli cell adhesion (haemagglutination) mediated by colonisation factor antigen 1 (CFA1) was determined by chromatographic fractionation of human whey proteins with Sephadex G-200, DEAE cellulose and heparin-sepharose [12].
  • The complex which is formed when excess regulatory subunits (r2) of aspartate transcarbamoylase (EC2.1.3.2) are added to a dilute solution of the catalytic subunit (c3) has been studied by gel-filtration on Sephadex G-200 [13].

Anatomical context of thiL


Associations of thiL with chemical compounds

  • T7 gene 6 exonuclease has been shown to have an RNase H activity as well as a double-strand specific DNase activity by the following experiments: The RNase H activity coelutes with the DNase activity from DEAE-cellulose, phosphocellulose, hydroxyapatite, and Sephadex G-200 columns [16].
  • To separate these aggregates, the unheated porin was suspended in 30% SDS, applied to a Sephadex G-200 gel filtration column, and eluted with 0.5% sodium deoxycholate [17].
  • The purification procedure involved phenol extraction, NaCl extraction, gel filtration on Sephadex G-100 and Sephadex G-200, and reversed-phase column chromatography on RPC-5 [18].
  • C*A remains stable following gel chromatography on Sephadex G-200 and sucrose gradient ultracentrifugation [19].
  • By contrast, chromatography of cell extracts with G-75 or G-200 columns revealed a singl-molecular-weight species of shikimate kinase activity with an apparent molecular weight of 20,000 [20].

Analytical, diagnostic and therapeutic context of thiL

  • The apparent molecular size of the complex was 140 kDa as determined by gel filtration on a Sephadex G-200 column [21].
  • Because of interference caused by humic acidlike substances, crude DNA extracts were purified with a Sephadex G-200 spun column before the PCR was begun [22].
  • Thermostabilities of kanamycin nucleotidyltransferase and of its mutants that became thermostable, in the free state, because of single-amino-acid replacements were studied after immobilization of the enzymes on cyanogen bromide-activated Sephadex G-200 particles [23].
  • GTP cyclohydrolase I, the first enzyme in the de novo biosynthesis of tetrahydrobiopterin, was enriched more than 13,000-fold from human liver by preparative isoelectric focusing using Sephadex G-200 SF gels [24].
  • The length of trp regRNA determined by filtration on Sephadex G-200 is 110-180 nucleotides [25].


  1. Reconstitution of RNase P activity from inactive RNA and protein. Kole, R., Altman, S. Proc. Natl. Acad. Sci. U.S.A. (1979) [Pubmed]
  2. Activation by thiol of the latent NAD glycohydrolase and ADP-ribosyltransferase activities of Bordetella pertussis toxin (islet-activating protein). Moss, J., Stanley, S.J., Burns, D.L., Hsia, J.A., Yost, D.A., Myers, G.A., Hewlett, E.L. J. Biol. Chem. (1983) [Pubmed]
  3. Partial purification and characterization of a ribonucleic acid N2-guanine methyltransferase associated with avian myeloblastosis virus. Taylor, M.J., Gantt, R. Biochemistry (1979) [Pubmed]
  4. Isolation, partial purification and preliminary characterization of a bacteriocin from Streptococcus mutans Rm-10. Fukushima, H., Kelstrup, J., Fukushima, S., Umemoto, T., Sagawa, H. Antonie Van Leeuwenhoek (1983) [Pubmed]
  5. 15N-labelling and preliminary heteronuclear NMR study of Desulfovibrio vulgaris Hildenborough cytochrome c553. Morelli, X., Dolla, A., Toci, R., Guerlesquin, F. Eur. J. Biochem. (1999) [Pubmed]
  6. Expression and characterization of mutant forms of the type I regulatory subunit of cAMP-dependent protein kinase. The effect of defective cAMP binding on holoenzyme activation. Woodford, T.A., Correll, L.A., McKnight, G.S., Corbin, J.D. J. Biol. Chem. (1989) [Pubmed]
  7. Serine and tyrosine protein kinase activities in Streptococcus pyogenes. Phosphorylation of native and synthetic peptides of streptococcal M proteins. Chiang, T.M., Reizer, J., Beachey, E.H. J. Biol. Chem. (1989) [Pubmed]
  8. Purification and properties of cytochrome b556 in the respiratory chain of aerobically grown Escherichia coli K12. Kita, K., Yamato, I., Anraku, Y. J. Biol. Chem. (1978) [Pubmed]
  9. 3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase. Purification, properties, and kinetics of the tyrosine-sensitive isoenzyme from Escherichia coli. Schoner, R., Herrmann, K.M. J. Biol. Chem. (1976) [Pubmed]
  10. Lysophospholipase of Escherichia coli. Doi, O., Nojima, S. J. Biol. Chem. (1975) [Pubmed]
  11. The hmc operon of Desulfovibrio vulgaris subsp. vulgaris Hildenborough encodes a potential transmembrane redox protein complex. Rossi, M., Pollock, W.B., Reij, M.W., Keon, R.G., Fu, R., Voordouw, G. J. Bacteriol. (1993) [Pubmed]
  12. Free secretory component and lactoferrin of human milk inhibit the adhesion of enterotoxigenic Escherichia coli. Giugliano, L.G., Ribeiro, S.T., Vainstein, M.H., Ulhoa, C.J. J. Med. Microbiol. (1995) [Pubmed]
  13. Further studies on aspartate transcarbamoylase: molecular weight of the c3r6 complex and analysis of succinate inhibition in the native enzyme. Chan, W.W. Can. J. Biochem. (1976) [Pubmed]
  14. Bacterial overexpression, purification, and reconstitution of the carnitine/acylcarnitine carrier from rat liver mitochondria. Indiveri, C., Iacobazzi, V., Giangregorio, N., Palmieri, F. Biochem. Biophys. Res. Commun. (1998) [Pubmed]
  15. Purification of horse eosinophil peroxidase. Jörg, A., Pasquier, J.M., Klebanoff, S.J. Biochim. Biophys. Acta (1982) [Pubmed]
  16. T7 gene 6 exonuclease has an RNase H activity. Shinozaki, K., Okazaki, T. Nucleic Acids Res. (1978) [Pubmed]
  17. Isolation and preliminary characterization of wild-type OmpC porin dimers from Escherichia coli K-12. Rocque, W.J., McGroarty, E.J. Biochemistry (1989) [Pubmed]
  18. Purification of the messenger ribonucleic acid for the lipoprotein of the Escherichia coli outer membrane. Wang, S.S., Pirtle, R., Pirtle, I., Small, M., Inouye, M. Biochemistry (1979) [Pubmed]
  19. Interaction between the antibiotic spiramycin and a ribosomal complex active in peptide bond formation. Dinos, G., Synetos, D., Coutsogeorgopoulos, C. Biochemistry (1993) [Pubmed]
  20. Aromatic amino acid biosynthesis: regulation of shikimate kinase in Escherichia coli K-12. Ely, B., Pittard, J. J. Bacteriol. (1979) [Pubmed]
  21. Identification and chemical synthesis of a ribosomal protein antigenic determinant in systemic lupus erythematosus. Elkon, K., Skelly, S., Parnassa, A., Moller, W., Danho, W., Weissbach, H., Brot, N. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  22. Detection of Escherichia coli in sewage and sludge by polymerase chain reaction. Tsai, Y.L., Palmer, C.J., Sangermano, L.R. Appl. Environ. Microbiol. (1993) [Pubmed]
  23. Does single-amino-acid replacement work in favor of or against improvement of the thermostability of immobilized enzyme? Koizumi, J., Zhang, M., Imanaka, T., Aiba, S. Appl. Environ. Microbiol. (1990) [Pubmed]
  24. Purification of GTP cyclohydrolase I from human liver and production of specific monoclonal antibodies. Schoedon, G., Redweik, U., Curtius, H.C. Eur. J. Biochem. (1989) [Pubmed]
  25. Punctuation of transcription in vitro of the tryptophan operon of Escherichia coli. A novel type of control of transcription. Pannekoek, H., Brammar, W.J., Pouwels, P.H. Mol. Gen. Genet. (1975) [Pubmed]
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