Disease relevance of Photometry

• Single turnover experiments monitored by multiwavelength photometry were performed with the recombinant lumazine synthase of Bacillus subtilis [1].
• Detection of false lithium readings in whole-blood specimens from cadavers by flame photometry [2].
• Patients with retinitis pigmentosa (RP) or Usher syndrome and normal subjects had MP optical density profiles measured with heterochromatic flicker photometry [3].
• 1 The effect of topical arachidonic acid on anthralin inflammation was studied using sequential measurements of erythema (reflectance photometry) and oedema (calipers) [4].
• Further developments in the quantification of small RNA viruses by U.V. photometry of sucrose density gradients [5].

High impact information on Photometry

• Fluorescence photometry and radioimmunoassays have been used to follow quantitative and topographic changes in the H36 determinant during myogenesis [6].
• The response of the mutant RyR1 Ca2+ channel to the agonists halothane and caffeine in a Ca2+ photometry assay was completely abolished [7].
• We have taken advantage of laser photometry, the Ca2(+)-sensitive dye Indo-1 that allows ratio imaging, and confocal microscopy to eliminate the influences of unequal cell geometry and dye distribution [8].
• We used photometry to measure the dynamics of cytosolic Ca(2+) ([Ca(2+)](i)) clearance after brief, depolarization-induced Ca(2+) entry [9].
• After about 48 h, intact cells were loaded with the fluorescent Ca2+ indicator, fura-2, and intracellular Ca2+ release, induced by caffeine or halothane, was measured by photometry [10].

Chemical compound and disease context of Photometry

• Plasma levels of histamine (HI) were determined by fluorescence photometry in 12 patients with old myocardial infarction, 10 patients with stable effort angina pectoris and 10 control subjects without coronary arterial lesion [11].

Biological context of Photometry

• Cells injected with Pur alpha during S or G2 phases were efficiently blocked with a 4N (G2 phase) DNA level, as determined by quantitative DNA photometry of individual cells [12].
• The left and right renal blood flows were measured by electromagnetic flowmeters (wrap-round probes), glomerular filtration rate by creatinine clearance, urinary sodium by flame photometry and solute excretion by osmometry [13].
• The renal blood flow was measured by an electromagnetic flow meter (wrap-round probe), glomerular filtration rate by creatinine clearance, urinary sodium excretion by flame photometry and osmolar excretion by osmometry [14].
• PMCA-mediated Ca2+ extrusion was recorded following brief trains of action potentials using indo-1-based photometry in the presence of cyclopiazonic acid [15].
• In the hypertrophic nonunions, 20 muamps of direct current for eight weeks produced an increase in bone formation compared to the opposite control limb by radiography, photometry, point counting of new bone, and growth rate by sequential fluorochrome labeling and the dynamic uptake of 99mTc-labeled methylene disphosphonate [16].

Anatomical context of Photometry

• 1. Integrating flame photometry was employed for measuring the mobilization of sodium from rat pancreatic islets after substitution of extracellular Na+ by N-methylglucamine [17].
• The calcium ion (Ca2+) and potassium ion (K+) content in the ashed material from red blood cells was determined by flame photometry in 61 essential hypertensives, 11 renal hypertensives and in 47 normotensive controls, and intracellular K+ concentration was measured in the haemolysate [18].
• A computer-assisted imaging photometry system was used to quantitate lysozyme and nonspecific esterase-neutral protease levels within individual macrophages following ingestion of nocardiae [19].
• Balanced salt solution (BSS((R))) served as a control to evaluate corneal endothelial cell damage using Janus Green photometry [20].
• Mononuclear leukocytes were incubated with or without aldosterone (1.4 nM) in RPMI-1640 for 1 h at 37 degrees C. After two washes in isotonic MgCl2 the wet cell pellets were weighted and intracellular sodium and potassium determined by flame photometry, results are expressed as mmol/kg wet cells [21].

Associations of Photometry with chemical compounds

• The study demonstrates the usefulness of the cellular Ca2+ photometry assay in the assessment of the sensitivity to caffeine and halothane of specific ryanodine receptor mutants [10].
• Plasma lithium stability and a comparison of flame photometry and atomic absorption spectrophotometry analysis [22].
• Bumetanide was analyzed by HPLC, and sodium and potassium was analyzed by flame photometry [23].
• Quantitative analyses of sweat for sodium by flame photometry and for chloride by silver titration and ion-sleective electrodes are now used as confirmatory tests [24].
• Total bilirubin measurement by photometry on a blood gas analyzer: potential for use in neonatal testing at the point of care [25].

Gene context of Photometry

• Our recent work has tested predictions of these models via kinetic analysis of drug transport and single-cell photometry analysis of pHi, delta psi, and volume regulation in novel MDR and CFTR transfectants that have not been exposed to chemotherapeutic drugs prior to analysis [26].
• Semiautomated HLA-DQB1 typing by fluorescent dye photometry of amplified DNA on microtiter plates [27].
• The activity of glutamate dehydrogenase (GDH), an important enzyme in carbon and nitrogen metabolism, is routinely assayed by photometry [28].
• Photometry indicated that these lamps emit substantial levels of UV-B (280-320 nm) radiation, which is toxic to patients with SLE [29].
• Analysis of EUV photometry of the He-rich DO white dwarf MCT 0501-2858 and the H-rich DA white dwarf MCT 0455-2812 along one line of sight and of the DA white dwarfs HZ 43 and GD 153 near the north Galactic pole indicates that the overall minimum column density of the neutral material centered on the Sun is N(H I)= 0.5-1.0 x 10(18) cm-2 [30].

Analytical, diagnostic and therapeutic context of Photometry

• Enzymatic determinations of sodium, potassium, and chloride in serum compared with determinations by flame photometry, coulometry, and ion-selective electrodes [31].
• Nystatin-induced potassium efflux from Saccharomyces cerevisiae measured by flame photometry: a potential bioassay approach [32].
• Intracellular Ca2+ ([Ca2+]i) dynamics were studied in identified rat gonadotropes using the whole-cell patch-clamp technique in conjunction with Indo-1 photometry [33].
• Qualitative and quantitative determination of biologically active low-molecular-mass thiols in human blood by reversed-phase high-performance liquid chromatography with photometry and fluorescence detection [34].
• Characterization of the refolding and reassembly of an integral membrane protein OmpF porin by low-angle laser light scattering photometry coupled with high-performance gel chromatography [35].

References