Disease relevance of peroxyformic acid

• In line with the emerging epitope requirements of YL 1/2, the Escherichia coli rec A protein, the catalytic subunit of the cyclic AMP-dependent muscle protein kinase as well as performic acid-oxidized actin were recognized by YL 1/2 in immunoblots [1].
• Performic acid and peroxide oxidation procedures on 35S-labeled peptidoglycan from Fusobacterium nucleatum Fev1 resulted in low yields of cysteic acid (42 and 60%, respectively) and many other additional unidentified oxidation products [2].
• Sequence analysis was performed on fragments obtained from cleavage of intact and performic acid-oxidized hormone with lysyl endopeptidase, Staphylococcus aureus protease, and o-iodosobenzoic acid employing manual Edman degradation [3].
• Performic acid toxicity studies on stabilized HEp-2 cells [4].
• Disinfecting properties of performic acid against bacteriophage phi X 174 as a model of small envelope--free viruses [5].

High impact information on peroxyformic acid

• In contrast, a performic acid-oxidized CaM did not have a protective effect on spindle structure when microinjected into metaphase cells before nocodazole treatment [6].
• Since performic acid oxidized CEA also inhibits endocytosis, receptor binding is similarly independent of intact protein conformation [7].
• 5,5'-dithiobis-(2-nitrobenzoate) titrations of the native and the denatured enzyme together with analysis for cysteic acid after performic acid oxidation showed that the enzyme contained 12 free SH groups and three disulfide bonds/monomer [8].
• Nevertheless, neither native nor performic acid-oxidized RNase is a substrate for the protease [9].
• The enzyme contained an acyl carrier protein-like segment as indicated by incorporation of [1-14C] pantothenate into the 238-kDa protein and production of 1 mol of taurine/mol of the monomer upon hydrolysis of performic acid-oxidized enzyme [10].

Chemical compound and disease context of peroxyformic acid

• Since performic acid is used as a potent disinfectant, chemical sterilant and/or skin antiseptics, information related to its toxicity for living tissues was needed to evaluate the degree of its biotolerance and compare it to that of peracetic acid [4].

Biological context of peroxyformic acid

• Oxidation of the aminoacyl coenzyme with performic acid, followed by acid hydrolysis, yields 2',5'-anhydroriboflavin and some free riboflavin, products also obtained on similar treatment of FMN [11].
• The complete amino acid sequence of the hormone has been determined by Edman degradation of the S-carboxymethylated or performic acid-oxidized A and B chains, and of various tryptic peptides derived from the chains [12].
• Performic acid oxidation completely deacetylates the enzyme, suggesting the site of acetylation to be a cysteinyl sulfhydryl group [13].
• Tyrosine 49 was the only protein amino acid modified in the binding site photolabeling reaction as assessed by peptide mapping of the performic acid oxidized and trypsin-digested photolabeled protein using reverse phase high performance liquid chromatography [14].
• Endocytosis of CEA was independent of native protein conformation since performic acid oxidized CEA and glycopeptides produced by pepsin digestion were inhibitory [15].

Anatomical context of peroxyformic acid

• 4. Acetyl-CoA carboxylase from fat-cells previously incubated in medium containing [32P]phosphate was purified by immunoprecipitation and then digested with performic acid and trypsin before separation of the released phosphopeptides by two-dimensional analysis [16].
• Mouse SV3T3 actin and skeletal muscle actin were compared by fingerprint analysis of the tryptic peptides obtained from performic-acid-oxidized protein [17].
• It can be shown that oxidation of protein antigens (either by chlorination or performic acid treatment) allow protein unfolding and enhance both processing and exposure of immunogenic epitopes to specific T cells [18].
• Alteration of the tertiary structure of hGH, human chorionic somatomammotrophin, and ovine prolactin by performic acid oxidation destroys the mammary gland activities of these hormones [19].
• The cross reactivity of sera from rabbits sensitized to performic acid oxidized ferredoxin (O-Fd) and of spleen cells from mice sensitized to O-Fd was analysed using several chemically modified forms of ferredoxin in the complement fixation test and the in vitro lymphocyte stimulation assay [20].

Associations of peroxyformic acid with other chemical compounds

• A total of 20 sulfhydryl groups measured by titration of the reduced protein with 5,5'-dithiobis(2-nitrobenzoic acid) is in agreement with 20 cystic acid residues determined in hydrolysates of performic acid-oxidized protein [21].
• The chymotryptic and tryptic peptides from the performic acid oxidized toxin were separated by gel filtration on Sephadex G-25 and zone electrophoresis in columns of cellulose powder [22].
• The enzyme contained one 4'-phosphopantetheine residue per subunit (269 000), as determined by measurement of the taurine generated by hydrolysis of performic acid-treated enzyme [23].
• The thioether link in the FMN-containing peptides was converted to the sulfone by performic acid oxidation, as judged by characteristic absorbance changes and an increase in flavin fluorescence [24].
• While the acetylated enzyme formed upon incubation of HMG-CoA synthase with acetyl-CoA is labile to performic acid oxidation, the adduct formed upon 3-chloropropionyl-CoA inactivation is stable to such treatment [25].

Gene context of peroxyformic acid

• Oxidation of OT was well as AVP with performic acid abolished any PRL-releasing activity [26].
• The order of the fragments was deduced from peptides that overlap methionyl residues which were obtained by thermolysin digestion of performic acid-oxidized transferrin or by partial peptic hydrolysis of unmodified transferrin, together with other evidence [27].
• Treatment of beta END with performic acid caused a 60% loss in its ability to release PRL [26].
• Four tested monoclonal antibodies failed to react with bovine growth hormone and with hGH oxidized by performic acid [28].
• As with calmodulin's interactions with other calmodulin-dependent enzymes, the oxidation of the methionines of calmodulin by performic acid treatment decreases the ability of the methyltransferase to recognize and methylate calmodulin [29].

Analytical, diagnostic and therapeutic context of peroxyformic acid

• Reverse-phase HPLC elution behavior before and after performic acid oxidation and amino acid composition of the protein products led to their identification as NP-I-(9-93) (the 9-93 sequence) and [des-19,20]NP-I-(9-93) (the 9-93 sequence with the dipeptide 19-20 missing) for the more rapidly and more slowly formed species, respectively [30].
• Strong bases, boiling 3 N HCl, and performic acid did attack the sheath; in these cases, the array was systematically disassembled in a progressive manner, which was followed by electron microscopy [31].
• However, no cleavable [14C]phenylalanine could be detected after treatment with performic acid, indicating that the resulting mutant was unable to form thioester with phenylalanine [32].
• The identity of some of the disulfide-linked peptides was further verified by performic acid oxidation followed by sequence analysis of the resulting peptides [33].
• The resulting crude peptide mixture was oxidized by performic acid and the insulin A-chain, which contains no cleavage side for trypsin, was quantified by HPLC in the form of a tetrasulfonate derivative to reduce artefacts due to free -SH groups [34].

References