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Chemical Compound Review

Pyronine     (6-dimethylaminoxanthen-3- ylidene)...

Synonyms: Pyronine G, Pyronine Y, Pyronin- J, Pyronin- Y, Pyronin-?J, ...
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Disease relevance of Pyronine


High impact information on Pyronine

  • In determining whether Bcl-2 and Bcl-x(L) act in G(0) or G(1), forward scatter and pyronin Y fluorescence measurements indicated that Bcl-2 and Bcl-x(L) cells arrested more effectively in G(0) than controls, and were delayed in G(0)-G(1) transition [4].
  • DNA-bound Hoechst 33342 (blue), protein-fluorescein isothiocyanate (green), and RNA-bound pyronin Y (red) fluorescence measurements were correlated as each stained cell intersected three spatially separated laser beams [5].
  • Interestingly, the G(0) cells defined by their low levels of Hoechst 33342 and Pyronin Y staining, and reduced Ki67 and cyclin D expression (representing 21% of the cultured CB population) include some mature erythroid CFCs but very few primitive CFCs, LTC-ICs, or repopulating cells [6].
  • To test the hypothesis that primitive LTHC-ICs would reenter a state of relative quiescence after in vitro division, BM CD34(+) cells proliferating in ex vivo cultures were identified from their quiescent counterparts by a relative loss of membrane intercalating dye PKH2, and were further fractionated with Hst and PY [7].
  • Reticulocytes in fixed human blood samples were stained for RNA with the fluorescent dye pyronin Y and measured by flow cytometry [8].

Chemical compound and disease context of Pyronine


Biological context of Pyronine


Anatomical context of Pyronine


Associations of Pyronine with other chemical compounds


Gene context of Pyronine


Analytical, diagnostic and therapeutic context of Pyronine


  1. Staining with pyronin Y detects changes in conformation of RNA during mitosis and hyperthermia of CHO cells. Traganos, F., Crissman, H.A., Darzynkiewicz, Z. Exp. Cell Res. (1988) [Pubmed]
  2. Chromosomal aberrations and SCEs in Allium cepa root-tip cells treated with caffeine and pyronin Y. Cortés, F., Hazen, M.J. Mutat. Res. (1984) [Pubmed]
  3. Laryngeal epithelial changes on effects of smoking and drinking. Hirabayashi, H., Koshii, K., Uno, K., Ohgaki, H., Nakasone, Y., Fujisawa, T., Shono, N., Hinohara, T., Hirabayashi, K. Auris, nasus, larynx. (1990) [Pubmed]
  4. Bcl-xL/Bcl-2 coordinately regulates apoptosis, cell cycle arrest and cell cycle entry. Janumyan, Y.M., Sansam, C.G., Chattopadhyay, A., Cheng, N., Soucie, E.L., Penn, L.Z., Andrews, D., Knudson, C.M., Yang, E. EMBO J. (2003) [Pubmed]
  5. Normal and perturbed Chinese hamster ovary cells: correlation of DNA, RNA, and protein content by flow cytometry. Crissman, H.A., Darzynkiewicz, Z., Tobey, R.A., Steinkamp, J.A. J. Cell Biol. (1985) [Pubmed]
  6. Human hematopoietic stem cells stimulated to proliferate in vitro lose engraftment potential during their S/G(2)/M transit and do not reenter G(0). Glimm, H., Oh, I.H., Eaves, C.J. Blood (2000) [Pubmed]
  7. Functional heterogeneity of human CD34(+) cells isolated in subcompartments of the G0 /G1 phase of the cell cycle. Gothot, A., Pyatt, R., McMahel, J., Rice, S., Srour, E.F. Blood (1997) [Pubmed]
  8. Flow cytometry of reticulocytes applied to clinical hematology. Tanke, H.J., Rothbarth, P.H., Vossen, J.M., Koper, G.J., Ploem, J.S. Blood (1983) [Pubmed]
  9. Profile of cell cycle in hematopoietic malignancy by DNA/RNA quantitation using 7AAD/PY. Toba, K., Kishi, K., Koike, T., Winton, E.F., Takahashi, H., Nagai, K., Maruyama, S., Furukawa, T., Hashimoto, S., Masuko, M., Uesugi, Y., Kuroha, T., Tsukada, N., Shibata, A. Exp. Hematol. (1996) [Pubmed]
  10. The effects of pyronin on sprouting and regeneration of mouse motor nerves. Keynes, R.J. Brain Res. (1982) [Pubmed]
  11. Pyronin G as a fluorescent probe for quantitative determination of the membrane potential of mitochondria. Tomov, T.C. J. Biochem. Biophys. Methods (1986) [Pubmed]
  12. Functional heterogeneity within rhodamine123(lo) Hoechst33342(lo/sp) primitive hemopoietic stem cells revealed by pyronin Y. Hüttmann, A., Liu, S.L., Boyd, A.W., Li, C.L. Exp. Hematol. (2001) [Pubmed]
  13. Assessment of proliferative and colony-forming capacity after successive in vitro divisions of single human CD34+ cells initially isolated in G0. Gothot, A., Pyatt, R., McMahel, J., Rice, S., Srour, E.F. Exp. Hematol. (1998) [Pubmed]
  14. A simple fluorescent staining procedure for micronuclei and RNA in erythrocytes using Hoechst 33258 and pyronin Y. MacGregor, J.T., Wehr, C.M., Langlois, R.G. Mutat. Res. (1983) [Pubmed]
  15. Demonstration of lipofuscin and Nissl bodies in crystal violet stained sections using a fluorescence technique or pyronin Y stain. Terr, L.I. Stain technology. (1986) [Pubmed]
  16. Age-related changes in the epithelium of the monkey larynx. Stearns, M.P., Cummings, C.W. The Annals of otology, rhinology, and laryngology. (1982) [Pubmed]
  17. Clinical flow cytometric reticulocyte analysis. Davis, B.H., Bigelow, N.C. Pathobiology (1990) [Pubmed]
  18. Effects of mounting media on fading of toluidine blue and pyronin G staining in epoxy sections. Schmolke, C. Biotechnic & histochemistry : official publication of the Biological Stain Commission. (1993) [Pubmed]
  19. Tyrosine hydroxylase- and/or aromatic L-amino acid decarboxylase-containing cells in the suprachiasmatic nucleus of the Syrian hamster (Mesocricetus auratus). Novak, C.M., Nunez, A.A. J. Chem. Neuroanat. (1998) [Pubmed]
  20. Phenotypic and functional analyses of CD34NEG hematopoietic precursors from mobilized peripheral blood. Dooley, D.C., Oppenlander, B.K. Methods Mol. Biol. (2004) [Pubmed]
  21. T cell reactivity to penicillin: phenotypic analysis of in vitro activated cell subsets. Koponen, M., Pichler, W.J., de Weck, A.L. J. Allergy Clin. Immunol. (1986) [Pubmed]
  22. Pyronin Y as a fluorescent stain for paraffin sections. Li, B., Wu, Y., Gao, X.M. Histochem. J. (2002) [Pubmed]
  23. Purity of commercial non-certified European samples of Pyronin Y. Lyon, H., Andersen, A.P., Andersen, I., Clausen, P.P., Herold, B. Histochem. J. (1982) [Pubmed]
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