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Chemical Compound Review

Bisbenzimide     2-[2-(4-ethoxyphenyl)-3H- benzoimidazol-5...

Synonyms: Hoechst-33342, hoechst33342, SureCN38686, Hoechst 33342, CHEMBL343002, ...
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Disease relevance of Bisbenzimide


High impact information on Bisbenzimide

  • We previously described a method for isolating murine hematopoietic stem cells capable of reconstituting lethally irradiated recipients, which depends solely on dual-wavelength flow cytometric analysis of murine bone marrow cells stained with the fluorescent DNA-binding dye Hoechst 33342 [6].
  • By combining cell surface staining with fluorochrome-conjugated monoclonal antibodies and Hoechst 33342 dye supravital staining, Matsuzaki et al. have succeeded in enriching hematopoietic stem cells (HSCs) essentially to homogeneity [7].
  • While experimenting with staining of murine bone marrow cells with the vital dye, Hoechst 33342, we discovered that display of Hoechst fluorescence simultaneously at two emission wavelengths revealed a small and distinct subset of whole bone marrow cells that had phenotypic markers of multipotential HSC [8].
  • The use of a dye, Hoechst 33342, which stains DNA stoichiometrically, allowed the simultaneous analysis of immunofluorescence and cell cycle position of individual cells [9].
  • Therefore, the aim of this study was to explore the hypothesis that viable intestinal stem cells can be isolated as a side population (SP) by fluorescence-activated cell sorting after staining with the DNA-binding dye Hoechst 33342 [10].

Chemical compound and disease context of Bisbenzimide


Biological context of Bisbenzimide


Anatomical context of Bisbenzimide


Associations of Bisbenzimide with other chemical compounds


Gene context of Bisbenzimide


Analytical, diagnostic and therapeutic context of Bisbenzimide


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