The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Chemical Compound Review

AC1NS0GT     4-[5-[6-(4-methylpyrazin-1- yl)-1H...

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of HOECHST 33258


High impact information on HOECHST 33258

  • Use of the bisbenzimidazole dye, Hoechst 33258, for chromosome staining after gentle fixation made possible the rapid identification and correlation of mitotic phase with surface properties [6].
  • Apoptosis was measured by two methods: 1) quantitative fluorescence microscopy of nuclear chromatin condensation in cells stained with the dye, bisbenzamide trihydrochloride (Hoechst-33258), and 2) terminal deoxynucleotidyl transferase (TdT) labeling of the 3'-OH ends of DNA fragments produced in apoptotic cells [7].
  • METHODS: Morphological changes were shown by light and electron microscopy, chromatin condensation and DNA breaks were assessed using Hoechst 33258 staining and DNA nickend labeling, and DNA fragmentation was characterized b gel electrophoresis [8].
  • The DNA-binding fluorophore Hoechst 33258 revealed chromatin condensation patterns consistent with apoptotic cell death in all cell types except MCF-7 cells [9].
  • The S6 peptide is shown to be a competitive inhibitor of Hoechst 33258, and it is concluded that the SPKK repeat binds to DNA in its minor groove with a binding constant, KS6 = 1.67 X 10(10) M-1 [10].

Chemical compound and disease context of HOECHST 33258


Biological context of HOECHST 33258


Anatomical context of HOECHST 33258


Associations of HOECHST 33258 with other chemical compounds


Gene context of HOECHST 33258


Analytical, diagnostic and therapeutic context of HOECHST 33258


  1. Apoptosis and loss of renal tissue in polycystic kidney diseases. Woo, D. N. Engl. J. Med. (1995) [Pubmed]
  2. Expression of p57(KIP2) potently blocks the growth of human astrocytomas and induces cell senescence. Tsugu, A., Sakai, K., Dirks, P.B., Jung, S., Weksberg, R., Fei, Y.L., Mondal, S., Ivanchuk, S., Ackerley, C., Hamel, P.A., Rutka, J.T. Am. J. Pathol. (2000) [Pubmed]
  3. Binding of Hoechst 33258 to the TAR RNA of HIV-1. Recognition of a pyrimidine bulge-dependent structure. Dassonneville, L., Hamy, F., Colson, P., Houssier, C., Bailly, C. Nucleic Acids Res. (1997) [Pubmed]
  4. In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Chen, T.R. Exp. Cell Res. (1977) [Pubmed]
  5. Membrane-permeant, DNA-binding agents alter intracellular trafficking and increase the transfection efficiency of complexed plasmid DNA. Fong, S., Liu, Y., Heath, T., Fong, P., Liggitt, D., Debs, R.J. Mol. Ther. (2004) [Pubmed]
  6. Surface functions during Mitosis I: phagocytosis, pinocytosis and mobility of surface-bound Con A. Berlin, R.D., Oliver, J.M., Walter, R.J. Cell (1978) [Pubmed]
  7. Potentiation of apoptosis by treatment with the protein kinase C-specific inhibitor safingol in mitomycin C-treated gastric cancer cells. Schwartz, G.K., Haimovitz-Friedman, A., Dhupar, S.K., Ehleiter, D., Maslak, P., Lai, L., Loganzo, F., Kelsen, D.P., Fuks, Z., Albino, A.P. J. Natl. Cancer Inst. (1995) [Pubmed]
  8. Mechanisms of cell death after pancreatic duct obstruction in the opossum and the rat. Gukovskaya, A.S., Perkins, P., Zaninovic, V., Sandoval, D., Rutherford, R., Fitzsimmons, T., Pandol, S.J., Poucell-Hatton, S. Gastroenterology (1996) [Pubmed]
  9. Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. Oberhammer, F., Wilson, J.W., Dive, C., Morris, I.D., Hickman, J.A., Wakeling, A.E., Walker, P.R., Sikorska, M. EMBO J. (1993) [Pubmed]
  10. SPKK, a new nucleic acid-binding unit of protein found in histone. Suzuki, M. EMBO J. (1989) [Pubmed]
  11. Highly potent synthetic polyamides, bisdistamycins, and lexitropsins as inhibitors of human immunodeficiency virus type 1 integrase. Neamati, N., Mazumder, A., Sunder, S., Owen, J.M., Tandon, M., Lown, J.W., Pommier, Y. Mol. Pharmacol. (1998) [Pubmed]
  12. Enhanced P53 and BAX gene expression and apoptosis in A549 cells by cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP. Kosmider, B., Wojcik, I., Osiecka, R., Bartkowiak, J., Zyner, E., Ochocki, J., Liberski, P. Investigational new drugs. (2005) [Pubmed]
  13. Transcriptional regulation of differentiation, selective toxicity and ATGCAAAT binding of bisbenzimidazole derivatives in human melanoma cells. Wong, S.S., Sturm, R.A., Michel, J., Zhang, X.M., Danoy, P.A., McGregor, K., Jacobs, J.J., Kaushal, A., Dong, Y., Dunn, I.S. Biochem. Pharmacol. (1994) [Pubmed]
  14. Temporal profile of apoptotic-like changes in neurons and astrocytes following controlled cortical impact injury in the rat. Newcomb, J.K., Zhao, X., Pike, B.R., Hayes, R.L. Exp. Neurol. (1999) [Pubmed]
  15. Inhibition of mitogen-activated protein kinase kinase enhances apoptosis induced by arsenic trioxide in human breast cancer MCF-7 cells. Ye, J., Li, A., Liu, Q., Wang, X., Zhou, J. Clin. Exp. Pharmacol. Physiol. (2005) [Pubmed]
  16. Mouse satellite DNA, centromere structure, and sister chromatid pairing. Lica, L.M., Narayanswami, S., Hamkalo, B.A. J. Cell Biol. (1986) [Pubmed]
  17. Assignment of human beta-, gamma-, and delta-globin genes to the short arm of chromosome 11 by chromosome sorting and DNA restriction enzyme analysis. Lebo, R.V., Carrano, A.V., Burkhart-Schultz, K., Dozy, A.M., Yu, L.C., Kan, Y.W. Proc. Natl. Acad. Sci. U.S.A. (1979) [Pubmed]
  18. Quantitative karyotyping of human chromosomes by dual beam flow cytometry. Langlois, R.G., Yu, L.C., Gray, J.W., Carrano, A.V. Proc. Natl. Acad. Sci. U.S.A. (1982) [Pubmed]
  19. High-resolution chromosomal localization of human genes for amylase, proopiomelanocortin, somatostatin, and a DNA fragment (D3S1) by in situ hybridization. Zabel, B.U., Naylor, S.L., Sakaguchi, A.Y., Bell, G.I., Shows, T.B. Proc. Natl. Acad. Sci. U.S.A. (1983) [Pubmed]
  20. Behavior of centrosomes during fertilization and cell division in mouse oocytes and in sea urchin eggs. Schatten, H., Schatten, G., Mazia, D., Balczon, R., Simerly, C. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  21. Individual microglia move rapidly and directly to nerve lesions in the leech central nervous system. McGlade-McCulloh, E., Morrissey, A.M., Norona, F., Muller, K.J. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  22. Transforming growth factor beta 1 induces apoptotic cell death in cultured human gastric carcinoma cells. Yanagihara, K., Tsumuraya, M. Cancer Res. (1992) [Pubmed]
  23. The role of c-mos proto-oncoprotein in mammalian meiotic maturation. Zhao, X., Singh, B., Batten, B.E. Oncogene (1991) [Pubmed]
  24. Effects of Hoechst 33258 on different cell cycle events. I. Inhibition of synthetic activities in bone-marrow cells of the mole rat Bandicota bengalensis. Dubey, D.D., Raman, R. Exp. Cell Res. (1983) [Pubmed]
  25. Cytoplasmic reorganization during the resumption of meiosis in cultured preovulatory rat oocytes. Albertini, D.F. Dev. Biol. (1987) [Pubmed]
  26. Characterization of the minor groove environment in a drug-DNA complex: bisbenzimide bound to the poly[d(AT)].poly[d(AT)]duplex. Jin, R., Breslauer, K.J. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  27. Chromosome heteromorphism quantified by high-resolution bivariate flow karyotyping. Trask, B., van den Engh, G., Mayall, B., Gray, J.W. Am. J. Hum. Genet. (1989) [Pubmed]
  28. Flavopiridol binds to duplex DNA. Bible, K.C., Bible, R.H., Kottke, T.J., Svingen, P.A., Xu, K., Pang, Y.P., Hajdu, E., Kaufmann, S.H. Cancer Res. (2000) [Pubmed]
  29. Interferon gamma (IFNgamma ) and tumor necrosis factor alpha synergism in ME-180 cervical cancer cell apoptosis and necrosis. IFNgamma inhibits cytoprotective NF-kappa B through STAT1/IRF-1 pathways. Suk, K., Chang, I., Kim, Y.H., Kim, S., Kim, J.Y., Kim, H., Lee, M.S. J. Biol. Chem. (2001) [Pubmed]
  30. The binding mode of drugs to the TAR RNA of HIV-1 studied by electric linear dichroism. Bailly, C., Colson, P., Houssier, C., Hamy, F. Nucleic Acids Res. (1996) [Pubmed]
  31. Down-regulation of X-linked inhibitor of apoptosis protein induces apoptosis in chemoresistant human ovarian cancer cells. Sasaki, H., Sheng, Y., Kotsuji, F., Tsang, B.K. Cancer Res. (2000) [Pubmed]
  32. Apoptosis and proliferation of endothelial cells in early atherosclerotic lesions: possible role of oxidised LDL. Norata, G.D., Tonti, L., Roma, P., Catapano, A.L. Nutrition, metabolism, and cardiovascular diseases : NMCD. (2002) [Pubmed]
  33. Hoechst 33342-induced apoptosis is associated with intracellular accumulation of E2F-1 protein in BC3H-1 myocytes and HL-60 cells. Zhang, X., Kiechle, F.L. Arch. Pathol. Lab. Med. (2001) [Pubmed]
  34. Expression and identification of a novel apoptosis gene Spata17 (MSRG-11) in mouse spermatogenic cells. Deng, Y., Hu, L.S., Lu, G.X. Acta Biochim. Biophys. Sin. (Shanghai) (2006) [Pubmed]
  35. Apoptosis induced by inhibition of cyclic AMP response element-binding protein in vascular smooth muscle cells. Tokunou, T., Shibata, R., Kai, H., Ichiki, T., Morisaki, T., Fukuyama, K., Ono, H., Iino, N., Masuda, S., Shimokawa, H., Egashira, K., Imaizumi, T., Takeshita, A. Circulation (2003) [Pubmed]
  36. Rapid fluorescence-based assay for radiosensitivity and chemosensitivity testing in mammalian cells in vitro. Begg, A.C., Mooren, E. Cancer Res. (1989) [Pubmed]
  37. Attenuated acute liver injury in mice by naked hepatocyte growth factor gene transfer into skeletal muscle with electroporation. Xue, F., Takahara, T., Yata, Y., Minemura, M., Morioka, C.Y., Takahara, S., Yamato, E., Dono, K., Watanabe, A. Gut (2002) [Pubmed]
  38. Cooperativity in drug-DNA recognition: a molecular dynamics study. Harris, S.A., Gavathiotis, E., Searle, M.S., Orozco, M., Laughton, C.A. J. Am. Chem. Soc. (2001) [Pubmed]
  39. Paclitaxel induces apoptosis in Saos-2 cells with CD95L upregulation and Bcl-2 phosphorylation. Pucci, B., Bellincampi, L., Tafani, M., Masciullo, V., Melino, G., Giordano, A. Exp. Cell Res. (1999) [Pubmed]
WikiGenes - Universities