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Chemical Compound Review

Tricine     2-[[1,3-dihydroxy-2- (hydroxymethyl)propan...

Synonyms: AG-G-00920, ACMC-209ukq, CHEBI:39063, T0377_SIGMA, T1074_SIGMA, ...
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Disease relevance of Tricine

  • Ca2+ in the presence of N-[tris(hydroxymethyl)methyl]glycine (Tricine) buffer causes the formation of blebs in the membrane but does not cause changes in the intramembrane particle pattern or induce fusion [1].
  • Silver-stained tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles of lipopolysaccharides from other E. coli K serotypes showed that all strains with group IB K antigens expressed some K(LPS) [2].
  • We have mapped the sites of ischemia/reperfusion-induced phosphorylation of cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of Blue Native gel/Tricine gel electrophoresis and nano-LC-MS/MS approaches [3].
  • Lipopolysaccharide (LPS) extracts of reference strains and isolates of Bacteroides spp. prepared by the proteinase K method were resolved by tricine-sodium-dodecyl-sulphate-polyacrylamide gel electrophoresis and located by silver staining [4].
  • The in endometrial carcinoma cell-enriched culture media was characterized by immunoblots in tricine-supplied electrophoresis system and subsequent amino acid sequencing [5].

High impact information on Tricine

  • The immunoprecipitated peptide was isolated by electrophoresis in SDS/Tricine gels, eluted, and sequenced [6].
  • The present study demonstrates that the addition of ferredoxin and NADP+ in the presence of N-tris(hydroxymethyl)methylglycine (Tricine) buffer at pH 7.1 or higher mitigates the rapid loss of Biradical X [N,N'-bis(1-oxyl - 2,2,5,5 - tetramethylpyrroline-3-carboxy)-1,2-diaminoethane] and Monradical A (2,2,5,5-tetramethyl-3-carbamidpyrroline-1-oxyl) [7].
  • Families of fragments from 19-kDa membranes, including seven peptides of 7.6-11.7 kDa, have been separated by size-exclusion high performance liquid chromatography, concentrated, and resolved on 16.5% Tricine gels [8].
  • Perfusion with bicarbonate-free solutions containing tricine buffer (10 mM) reduced the membrane potential to -23 mV and reduced iron extraction by 18% [9].
  • PCR error was measured in low |Deltap K a| buffers containing tricine, EPPS and citrate, as well as otherwise identical buffers containing Tris [10].

Biological context of Tricine

  • Separation of proteolytic cleavage peptides (Lys-C endoproteinase digestion) by tricine SDS-PAGE and isoelectric focusing in connection with N-terminal amino acid sequencing revealed covalent modification of several active site peptides [11].
  • In vitro phosphorylation reactions indicated a thiol insensitive kinase activity to be present in the Tricine extract that was capable of phosphorylating histone H1 as well as purified LHCII [12].
  • A chelator, tricine, was necessary for maximal cell densities,, probably to solubilize trace ions and make them more available to the bacteria [13].
  • The method involves electrophoretic desalting by miniaturized Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis and in situ alkylation after electro-transfer onto polyvinylidene difluoride membranes [14].
  • The visualization of peptide hydrolysis was accomplished by Tricine gel electrophoresis [15].

Anatomical context of Tricine

  • The cell wall porin was purified to homogeneity and had an apparent molecular mass of about 87 kDa on tricine-containing SDS-PAGE [16].
  • We investigated this phenomenon with Arachis hypogea thylakoids prepared with (Th:A) or without (Th:B) tricine, where tricine is known for removing peripheral proteins from thylakoids [12].
  • Tricine as coligand showed higher activity levels in muscle, blood, and liver, whereas tricine-nicotinic acid produced significant levels of activity in the gastrointestinal tract [17].
  • Colcemid was added either to the medium in which larvae were cultured or to Tricine buffer in which isolated testes were incubated [18].
  • Since lysosomes are the principal sites of intracellular catabolism of proteins and peptides, 99mTc-HYNIC-labeled galactosyl-neoglycoalbumin (NGA) was prepared using tricine as a co-ligand to investigate the fate of the radiolabel after lysosomal proteolysis in hepatocytes [19].

Associations of Tricine with other chemical compounds

  • Thylakoid membranes and photosystem I, which was isolated by Triton X-100 fractionation, were treated with cross-linking reagents and were resolved using a Tricine/urea low-molecular-weight resolution gel system [20].
  • Based on tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of LPS in proteinase K-treated lysates of N. meningitidis H44/76, a quantitative comparison was made between the cytokine-inducing capacity of isolated and purified LPS and LPS-containing meningococci [21].
  • Biochemical analyses using tricine/SDS-polyacrylamide gel electrophoresis and western blotting with GSL II showed the GlcNAc-containing glycoproteins to be insoluble, with molecular masses ranging from 15 to 30 kDa [22].
  • The cysteines reacting with omeprazole were defined by proteolytic cleavage of 3H- or 14C-omeprazole-labeled enzyme followed by peptide sequencing of fragments separated on tricine gradient gels and transferred to PVDF membranes [23].
  • High resolution and reproducible separations of rt-PA glycopeptides carrying hybrid and complex type chains were obtained using either a 100 mM phosphate buffer, pH 6.6, or a 100 mM Tricine buffer, pH 8.2, containing 1.25 mM of putrescine [24].

Gene context of Tricine

  • Zn(2+) exhibited a prominent rescuing effect when added together with the excitotoxicant NMDA, which could be reverted by the Zn(2+)-chelator tricine [25].
  • The present study describes a method for preparation of (99m)Tc-EGF via the hydrazinopyridine-3-carboxylic acid (HYNIC) conjugation using tricine and ethylenediamine-N,N'-diacetic acid (EDDA) as co-ligands [26].
  • Analysis by two-dimensional isoelectric focusing/Tricine sodium dodecyl sulfide-polyacrylamide gel electrophoresis revealed the presence of further forms for Arabidopsis TOM20 [27].
  • This NACP oligomerization, appearing as a discrete ladder on a Tricine SDS-PAGE, was not observed with other A beta peptides such as the reverse peptide A beta35-25 and A beta1-40, indicating this process was specific not only for the C-terminal peptide sequence of the A beta but also for its orientation [28].
  • Tricine gel electrophoresis, followed by silver staining, showed that site-specific mutation in the waaC gene resulted in the expression of a severely truncated LOS compared to wild-type strain 81-176 [29].

Analytical, diagnostic and therapeutic context of Tricine

  • Insulin-stimulated autophosphorylation of the cytoplasmic juxtamembrane region of the human insulin receptor was examined by Tricine/SDS-PAGE [30].
  • The Arabidopsis mitochondrial proteome was analyzed by two-dimensional isoelectric focusing/ Tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 650 different proteins in a pI range of pH 3 to 10 were separated on single gels [31].
  • METHODS: Divalent (sc(Fv)(2)) and tetravalent ([sc(Fv)(2)](2)) scFv's of mAb CC49 were evaluated for radioimmunolocalization of LS-174T colon carcinoma xenografts in athymic mice. scFv's were radiolabeled with (99m)Tc by way of the bifunctional chelator succinimidyl-6-hydrazinonicotinate hydrochloride using tricine as the transchelator [32].
  • A radiolabeled peptide, approximately 6.5 kDa when analyzed by Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), was isolated by HPLC from a tryptic digest of the [3H]2EN-inactivated cytochrome P450 and NADPH-cytochrome P450 reductase [33].
  • Immunoblotting with several site-specific antibodies confirmed not only the presence of 4 kD A beta starting at the first amino acid of A beta but also 2 smaller A beta species with modification of their amino-termini in the highly resolutional Tris/Tricine gel system [34].


  1. Membrane ultrastructural changes during calcium phosphate-induced fusion of human erythrocyte ghosts. Zakai, N., Kulka, R.G., Loyter, A. Proc. Natl. Acad. Sci. U.S.A. (1977) [Pubmed]
  2. Formation of the K30 (group I) capsule in Escherichia coli O9:K30 does not require attachment to lipopolysaccharide lipid A-core. MacLachlan, P.R., Keenleyside, W.J., Dodgson, C., Whitfield, C. J. Bacteriol. (1993) [Pubmed]
  3. Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion. Fang, J.K., Prabu, S.K., Sepuri, N.B., Raza, H., Anandatheerthavarada, H.K., Galati, D., Spear, J., Avadhani, N.G. FEBS Lett. (2007) [Pubmed]
  4. Electrophoretic analysis of the lipopolysaccharides of Bacteroides spp. Maskell, J.P. Antonie Van Leeuwenhoek (1994) [Pubmed]
  5. Alternative gonadotropin-releasing hormone processing products secreted from endometrial carcinoma. Takagi, A., Imai, A., Tamaya, T. Oncol. Rep. (2000) [Pubmed]
  6. Direct identification of residues of the epidermal growth factor receptor in close proximity to the amino terminus of bound epidermal growth factor. Woltjer, R.L., Lukas, T.J., Staros, J.V. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  7. Stabilization of electron spin resonance probes for photosynthesis studies. Sun, A.S., Calvin, M. Proc. Natl. Acad. Sci. U.S.A. (1975) [Pubmed]
  8. Extensive digestion of Na+,K(+)-ATPase by specific and nonspecific proteases with preservation of cation occlusion sites. Capasso, J.M., Hoving, S., Tal, D.M., Goldshleger, R., Karlish, S.J. J. Biol. Chem. (1992) [Pubmed]
  9. Non-transferrin-bound iron uptake by rat liver. Role of membrane potential difference. Wright, T.L., Fitz, J.G., Weisiger, R.A. J. Biol. Chem. (1988) [Pubmed]
  10. Nucleotide analogs and new buffers improve a generalized method to enrich for low abundance mutations. Day, J.P., Hammer, R.P., Bergstrom, D., Barany, F. Nucleic Acids Res. (1999) [Pubmed]
  11. Suicidal inactivation of the rabbit 15-lipoxygenase by 15S-HpETE is paralleled by covalent modification of active site peptides. Wiesner, R., Suzuki, H., Walther, M., Yamamoto, S., Kuhn, H. Free Radic. Biol. Med. (2003) [Pubmed]
  12. Phosphorylation-dephosphorylation of light-harvesting complex II as a response to variation in irradiance is thiol sensitive and thylakoid sufficient: modulation of the sensitivity of the phenomenon by a peripheral component. Hazra, A., DasGupta, M. Biochemistry (2003) [Pubmed]
  13. Nutrition and enterotoxin synthesis by enterotoxigenic strains of Escherichia coli: defined medium for production of heat-stable enterotoxin. Alderete, J.F., Robertson, D.C. Infect. Immun. (1977) [Pubmed]
  14. In situ alkylation of cysteine residues in a hydrophobic membrane protein immobilized on polyvinylidene difluoride membranes by electroblotting prior to microsequence and amino acid analysis. Ploug, M., Stoffer, B., Jensen, A.L. Electrophoresis (1992) [Pubmed]
  15. Specificity of a Vibrio vulnificus aminopeptidase toward kinins and other peptidyl substrates. Richards, G.P., Nuñez, A. J. Bacteriol. (2006) [Pubmed]
  16. The cell wall porin of Nocardia farcinica: biochemical identification of the channel-forming protein and biophysical characterization of the channel properties. Riess, F.G., Lichtinger, T., Cseh, R., Yassin, A.F., Schaal, K.P., Benz, R. Mol. Microbiol. (1998) [Pubmed]
  17. 99mTc-HYNIC-[Tyr3]-octreotide for imaging somatostatin-receptor-positive tumors: preclinical evaluation and comparison with 111In-octreotide. Decristoforo, C., Melendez-Alafort, L., Sosabowski, J.K., Mather, S.J. J. Nucl. Med. (2000) [Pubmed]
  18. Chromosome segregation and spindle structure in crane fly spermatocytes following Colcemid treatment. LaFountain, J.R. Chromosoma (1985) [Pubmed]
  19. Intracellular metabolic fate of radioactivity after injection of technetium-99m-labeled hydrazino nicotinamide derivatized proteins. Ono, M., Arano, Y., Uehara, T., Fujioka, Y., Ogawa, K., Namba, S., Mukai, T., Nakayama, M., Saji, H. Bioconjug. Chem. (1999) [Pubmed]
  20. Organization of photosystem I polypeptides examined by chemical cross-linking. Armbrust, T.S., Chitnis, P.R., Guikema, J.A. Plant Physiol. (1996) [Pubmed]
  21. Contributions of Neisseria meningitidis LPS and non-LPS to proinflammatory cytokine response. Sprong, T., Stikkelbroeck, N., van der Ley, P., Steeghs, L., van Alphen, L., Klein, N., Netea, M.G., van der Meer, J.W., van Deuren, M. J. Leukoc. Biol. (2001) [Pubmed]
  22. Oligodendrocytes produce low molecular weight glycoproteins containing N-acetyl-D-glucosamine in their Golgi apparatus. Supler, M.L., Semple-Rowland, S.L., Streit, W.J. Glia (1994) [Pubmed]
  23. Membrane topology and omeprazole labeling of the gastric H+,K(+)-adenosinetriphosphatase. Besancon, M., Shin, J.M., Mercier, F., Munson, K., Miller, M., Hersey, S., Sachs, G. Biochemistry (1993) [Pubmed]
  24. Analysis of carbohydrate-mediated heterogeneity and characterization of N-linked oligosaccharides of glycoproteins by high performance capillary electrophoresis. Taverna, M., Baillet, A., Biou, D., Schlüter, M., Werner, R., Ferrier, D. Electrophoresis (1992) [Pubmed]
  25. Molecular interactions of the type 1 human immunodeficiency virus transregulatory protein Tat with N-methyl-d-aspartate receptor subunits. Chandra, T., Maier, W., König, H.G., Hirzel, K., Kögel, D., Schüler, T., Chandra, A., Demirhan, I., Laube, B. Neuroscience (2005) [Pubmed]
  26. [99mTc] HYNIC-hEGF, a potential agent for imaging of EGF receptors in vivo: preparation and pre-clinical evaluation. Babaei, M.H., Almqvist, Y., Orlova, A., Shafii, M., Kairemo, K., Tolmachev, V. Oncol. Rep. (2005) [Pubmed]
  27. Purification and characterization of the preprotein translocase of the outer mitochondrial membrane from Arabidopsis. Identification of multiple forms of TOM20. Werhahn, W., Niemeyer, A., Jänsch, L., Kruft, V., Schmitz, U.K., Braun, H. Plant Physiol. (2001) [Pubmed]
  28. Self-oligomerization of NACP, the precursor protein of the non-amyloid beta/A4 protein (A beta) component of Alzheimer's disease amyloid, observed in the presence of a C-terminal A beta fragment (residues 25-35). Paik, S.R., Lee, J.H., Kim, D.H., Chang, C.S., Kim, Y.S. FEBS Lett. (1998) [Pubmed]
  29. Mutation of waaC, encoding heptosyltransferase I in Campylobacter jejuni 81-176, affects the structure of both lipooligosaccharide and capsular carbohydrate. Kanipes, M.I., Papp-Szabo, E., Guerry, P., Monteiro, M.A. J. Bacteriol. (2006) [Pubmed]
  30. Insulin stimulates serine and tyrosine phosphorylation in the juxtamembrane region of the insulin receptor. Feener, E.P., Backer, J.M., King, G.L., Wilden, P.A., Sun, X.J., Kahn, C.R., White, M.F. J. Biol. Chem. (1993) [Pubmed]
  31. Proteomic approach to identify novel mitochondrial proteins in Arabidopsis. Kruft, V., Eubel, H., Jänsch, L., Werhahn, W., Braun, H.P. Plant Physiol. (2001) [Pubmed]
  32. 99mTc-labeled divalent and tetravalent CC49 single-chain Fv's: novel imaging agents for rapid in vivo localization of human colon carcinoma. Goel, A., Baranowska-Kortylewicz, J., Hinrichs, S.H., Wisecarver, J., Pavlinkova, G., Augustine, S., Colcher, D., Booth, B.J., Batra, S.K. J. Nucl. Med. (2001) [Pubmed]
  33. Mechanism-based inactivation of cytochrome P450 2B1 by 2-ethynylnaphthalene: identification of an active-site peptide. Roberts, E.S., Hopkins, N.E., Alworth, W.L., Hollenberg, P.F. Chem. Res. Toxicol. (1993) [Pubmed]
  34. Modified amyloid beta protein ending at 42 or 40 with different solubility accumulates in the brain of Alzheimer's disease. Harigaya, Y., Shoji, M., Kawarabayashi, T., Kanai, M., Nakamura, T., Iizuka, T., Igeta, Y., Saido, T.C., Sahara, N., Mori, H. Biochem. Biophys. Res. Commun. (1995) [Pubmed]
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