The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

Chrna3  -  cholinergic receptor, nicotinic, alpha...

Mus musculus

Synonyms: (a)3, A730007P14Rik, Acra-3, Acra3, Neuronal acetylcholine receptor subunit alpha-3, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Chrna3


High impact information on Chrna3

  • These effects require alpha 3 beta 1 integrin-mediated binding of TIMP-2 to endothelial cells [6].
  • We show that, in vivo, stem cells express higher levels of the alpha 2 beta 1 and alpha 3 beta 1 integrins than transit-amplifying cells and that this can be used both to determine the location of stem cells within the epidermis and to isolate them directly from the tissue [7].
  • Fourth, an independently derived V alpha 3-expressing T cell clone (2C: alloreactive to Ld) showed a response to arsonate/Ld [8].
  • We suggest that V alpha 3 encodes a protein sequence with a binding site for the arsonate hapten [8].
  • Third, a V alpha 3-containing alpha chain (Ar-5: arsonate/I-Ad) transferred arsonate responsiveness to an appropriate recipient T cell (O3: ovalbumin/I-Ad) [8].

Biological context of Chrna3

  • In this study, we show that the genes coding for the neuronal nicotinic acetylcholine receptor alpha 2, alpha 3 and beta 2 subunits are dispersed on three different mouse chromosomes, viz [9].
  • We prepared a panel of mouse monoclonal antibodies specific for the A and B variants of the alpha 3 subunit to study their tissue distribution [10].
  • The alpha subunits of the laminin-binding integrins alpha 3 beta 1, alpha 6 beta 1, and alpha 7 beta 1 have homologous sequences and are similar in structure [10].
  • Recently, Kimura noted that the mouse globin pseudogene, psi alpha 3, evolved faster than the normal mouse alpha 1 gene, although he did not compute the substitution rate [11].
  • The time course of adipogenesis declined from 7-10 days in controls to roughly 3 days in cultures treated with antisense-Gs alpha oligodeoxynucleotides, whereas oligodeoxynucleotides, antisense to Gi alpha 1, Gi alpha 3, and sense and missense to Gs alpha, had no such effect [12].

Anatomical context of Chrna3

  • One of the alpha-tubulin isotypes described is expressed exclusively in testis and is encoded by two closely related genes (M alpha 3 and M alpha 7) which have homologous 3' untranslated regions but which differ at multiple third codon positions and in their 5' untranslated regions [13].
  • Expression of the two variant forms of alpha 3 in K562 cells revealed that the ligand-binding specificities of alpha 3A beta 1 and alpha 3B beta 1 are identical: both bind human laminin-2 and -4, laminin-5, and laminins isolated from bovine kidney, but not bovine laminin-2 and -4, mouse laminin-1, or human fibronectin [10].
  • The alpha 3 domain of the Qa-2 molecule is defective for CD8 binding and cytotoxic T lymphocyte activation [14].
  • In IEC-6 cells, LPS caused an increase in the expression of alpha 3- and beta 1-integrins, a shift of beta 1-integrins from the cytoplasm to the plasma membrane and an increase in fibronectin bead adhesion during which beta 1-integrins accumulated underneath attached beads [15].
  • RESULTS: Newborn intestinal injury was associated with decreased intestinal restitution and increased alpha 3- and beta 1-integrin expression in the ileal mucosa, which also was observed after LPS injection [15].

Associations of Chrna3 with chemical compounds

  • While anabaseine has significant activity with muscle-type and neuronal alpha 3 beta 4 and alpha 4 beta 2 receptors, benzylidene anabaseine (BA) derivatives have high selectivity for the alpha 7 receptor subtype [16].
  • Thus, a V alpha 3 gene segment, in conjunction with at least two different J alpha segments (J alpha 20'(Ar-5) and J alpha pHDS58(2C)) and at least three different beta chains (V beta 2(Ar-5), V beta 6(O3), and V beta 8(2C], confers reactivity to arsonate in association with at least three different MHC proteins (I-Ad, I-Ak, and Ld) [8].
  • The T cell receptor V alpha 3 gene segment is associated with reactivity to p-azobenzenearsonate [8].
  • CONCLUSIONS: Enterocyte migration is inhibited by LPS through increased expression and function of alpha 3- and beta 1-integrins [15].
  • A schizophrenia-related sensorimotor deficit links alpha 3-containing GABAA receptors to a dopamine hyperfunction [17].

Physical interactions of Chrna3

  • (5) the TcR alpha 3 loop interacts with the central portion of the peptide and stacks against the beta 2 loop [18].

Regulatory relationships of Chrna3

  • Infection of L cells transfected with hybrid major histocompatibility complex class I molecules demonstrated that allelic control of susceptibility to MCMV mapped to the alpha 1 domain of Dd when in correct configuration with the alpha 2 and alpha 3 domains [19].
  • The RA-induced expression of alpha 3 transcripts accounts for a comparably small number of nAChR-containing cells (< 20%) of which half coexpress alpha 4 transcripts [20].

Other interactions of Chrna3

  • Chromosomal localization of the mouse genes coding for alpha 2, alpha 3, alpha 4 and beta 2 subunits of neuronal nicotinic acetylcholine receptor [9].
  • This result extends the similarity between alpha 3, alpha 6, and alpha 7 laminin receptor subunits and suggests a common ancestral gene [21].
  • Connexin alpha 3 (Cx46 or Gja3) gene targeted null mice developed lens nuclear cataracts shortly after birth [22].
  • The Lama3 locus encoding the alpha 3 subunit of nicein was mapped distal to ataxia and did not recombine with Tg9257 [23].
  • Segregation of restriction fragment length polymorphisms in backcross progeny of Mus musculus x Mus spretus mating confirmed this assignment and indicates that Atp4a and Atp1a3 (gene encoding the murine Na,K-ATPase alpha 3 subunit) are linked and separated by a distance of approximately 2 cM [24].

Analytical, diagnostic and therapeutic context of Chrna3

  • In addition, the relative amounts of mRNA for the major nicotinic receptor subunits (alpha 4 and beta 2), as well as for three additional minor subunits (alpha 2, alpha 3, and alpha 5), were determined by in situ hybridization histochemistry followed by quantitation of image intensity [25].
  • The isolated receptor was defined as the alpha 3 beta 1 integrin by immunoprecipitation with subunit-specific antibodies [26].
  • SDS-PAGE of 3H-mannose-labeled I-Ak immunoprecipitates revealed that the alpha-chain peak was divided into three regions (alpha 1, alpha 2, and alpha 3), each of which migrated as a distinct individual band when isolated and reelectrophoresed [27].
  • The mAb bound to murine (Mu) rIL-1 alpha 3 but not rMuIL-1 beta as assessed by both direct ELISA and immunoprecipitation [28].
  • However, RR3-16 did not react with all T cell lines and clones known to express genes from the V alpha 3 family. cDNA sequences of three independent RR3-16+ T cell hybridomas analyzed by polymerase chain reaction were identical to the previously published V alpha 3 sequence of the CTL clone C9 [29].


  1. A murine retrovirus induces proliferation of unique lymphoid cell lines expressing T-cell-receptor structures utilizing common variable region alpha and beta chain genes. O'Neill, H.C. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  2. Mouse alpha-globin genes and alpha-globin-like pseudogenes are not syntenic. Popp, R.A., Lalley, P.A., Whitney, J.B., Anderson, W.F. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]
  3. Defining a link between gap junction communication, proteolysis, and cataract formation. Baruch, A., Greenbaum, D., Levy, E.T., Nielsen, P.A., Gilula, N.B., Kumar, N.M., Bogyo, M. J. Biol. Chem. (2001) [Pubmed]
  4. The function and distinctive regulation of the integrin VLA-3 in cell adhesion, spreading, and homotypic cell aggregation. Weitzman, J.B., Pasqualini, R., Takada, Y., Hemler, M.E. J. Biol. Chem. (1993) [Pubmed]
  5. Induction of experimental bone metastasis in mice by transfection of integrin alpha 4 beta 1 into tumor cells. Matsuura, N., Puzon-McLaughlin, W., Irie, A., Morikawa, Y., Kakudo, K., Takada, Y. Am. J. Pathol. (1996) [Pubmed]
  6. TIMP-2 mediated inhibition of angiogenesis: an MMP-independent mechanism. Seo, D.W., Li, H., Guedez, L., Wingfield, P.T., Diaz, T., Salloum, R., Wei, B.Y., Stetler-Stevenson, W.G. Cell (2003) [Pubmed]
  7. Stem cell patterning and fate in human epidermis. Jones, P.H., Harper, S., Watt, F.M. Cell (1995) [Pubmed]
  8. The T cell receptor V alpha 3 gene segment is associated with reactivity to p-azobenzenearsonate. Tan, K.N., Datlof, B.M., Gilmore, J.A., Kronman, A.C., Lee, J.H., Maxam, A.M., Rao, A. Cell (1988) [Pubmed]
  9. Chromosomal localization of the mouse genes coding for alpha 2, alpha 3, alpha 4 and beta 2 subunits of neuronal nicotinic acetylcholine receptor. Bessis, A., Simon-Chazottes, D., Devillers-Thiéry, A., Guénet, J.L., Changeux, J.P. FEBS Lett. (1990) [Pubmed]
  10. The A and B variants of the alpha 3 integrin subunit: tissue distribution and functional characterization. de Melker, A.A., Sterk, L.M., Delwel, G.O., Fles, D.L., Daams, H., Weening, J.J., Sonnenberg, A. Lab. Invest. (1997) [Pubmed]
  11. Pseudogenes as a paradigm of neutral evolution. Li, W.H., Gojobori, T., Nei, M. Nature (1981) [Pubmed]
  12. Antisense oligodeoxynucleotides to GS protein alpha-subunit sequence accelerate differentiation of fibroblasts to adipocytes. Wang, H.Y., Watkins, D.C., Malbon, C.C. Nature (1992) [Pubmed]
  13. Six mouse alpha-tubulin mRNAs encode five distinct isotypes: testis-specific expression of two sister genes. Villasante, A., Wang, D., Dobner, P., Dolph, P., Lewis, S.A., Cowan, N.J. Mol. Cell. Biol. (1986) [Pubmed]
  14. The alpha 3 domain of the Qa-2 molecule is defective for CD8 binding and cytotoxic T lymphocyte activation. Teitell, M., Holcombe, H., Cheroutre, H., Aldrich, C.J., Stroynowski, I., Forman, J., Kronenberg, M. J. Exp. Med. (1993) [Pubmed]
  15. Increased expression and function of integrins in enterocytes by endotoxin impairs epithelial restitution. Qureshi, F.G., Leaphart, C., Cetin, S., Li, J., Grishin, A., Watkins, S., Ford, H.R., Hackam, D.J. Gastroenterology (2005) [Pubmed]
  16. Effects at a distance in alpha 7 nAChR selective agonists: benzylidene substitutions that regulate potency and efficacy. Papke, R.L., Meyer, E.M., Lavieri, S., Bollampally, S.R., Papke, T.A., Horenstein, N.A., Itoh, Y., Porter Papke, J.K. Neuropharmacology (2004) [Pubmed]
  17. A schizophrenia-related sensorimotor deficit links alpha 3-containing GABAA receptors to a dopamine hyperfunction. Yee, B.K., Keist, R., von Boehmer, L., Studer, R., Benke, D., Hagenbuch, N., Dong, Y., Malenka, R.C., Fritschy, J.M., Bluethmann, H., Feldon, J., Möhler, H., Rudolph, U. Proc. Natl. Acad. Sci. U.S.A. (2005) [Pubmed]
  18. TcR recognition of the MHC-peptide dimer: structural properties of a ternary complex. Vasmatzis, G., Cornette, J., Sezerman, U., DeLisi, C. J. Mol. Biol. (1996) [Pubmed]
  19. Murine cytomegalovirus interacts with major histocompatibility complex class I molecules to establish cellular infection. Wykes, M.N., Shellam, G.R., McCluskey, J., Kast, W.M., Dallas, P.B., Price, P. J. Virol. (1993) [Pubmed]
  20. Nicotinic receptor subunits alpha 3, alpha 4, and beta 2 and high affinity nicotine binding sites are expressed by P19 embryonal cells. Cauley, K., Marks, M., Gahring, L.C., Rogers, S.W. J. Neurobiol. (1996) [Pubmed]
  21. A new isoform of the laminin receptor integrin alpha 7 beta 1 is developmentally regulated in skeletal muscle. Collo, G., Starr, L., Quaranta, V. J. Biol. Chem. (1993) [Pubmed]
  22. Genetic factors influence cataract formation in alpha 3 connexin knockout mice. Gong, X., Agopian, K., Kumar, N.M., Gilula, N.B. Dev. Genet. (1999) [Pubmed]
  23. Location of the 9257 and ataxia mutations on mouse chromosome 18. Griffith, A.J., Radice, G.L., Burgess, D.L., Kohrman, D.C., Hansen, G.M., Justice, M.J., Johnson, K.R., Davisson, M.T., Meisler, M.H. Mamm. Genome (1996) [Pubmed]
  24. Genes encoding the H,K-ATPase alpha and Na,K-ATPase alpha 3 subunits are linked on mouse chromosome 7 and human chromosome 19. Malo, D., Gros, P., Bergmann, A., Trask, B., Mohrenweiser, H.W., Canfield, V.A., Levenson, R. Mamm. Genome (1993) [Pubmed]
  25. Nicotine binding and nicotinic receptor subunit RNA after chronic nicotine treatment. Marks, M.J., Pauly, J.R., Gross, S.D., Deneris, E.S., Hermans-Borgmeyer, I., Heinemann, S.F., Collins, A.C. J. Neurosci. (1992) [Pubmed]
  26. Subunit structure of a laminin-binding integrin and localization of its binding site on laminin. Gehlsen, K.R., Dickerson, K., Argraves, W.S., Engvall, E., Ruoslahti, E. J. Biol. Chem. (1989) [Pubmed]
  27. Murine I-Ak alpha-chain subspecies with glycosylation differences. Cowan, E.P., Schwartz, B.D., Cullen, S.E. J. Immunol. (1982) [Pubmed]
  28. Monoclonal antibodies to murine IL-1 alpha. Production, characterization, and inhibition of membrane-associated IL-1 activity. Fuhlbrigge, R.C., Sheehan, K.C., Schreiber, R.D., Chaplin, D.D., Unanue, E.R. J. Immunol. (1988) [Pubmed]
  29. Analysis of a monoclonal rat antibody directed to the alpha-chain variable region (V alpha 3) of the mouse T cell antigen receptor. Utsunomiya, Y., Bill, J., Palmer, E., Gollob, K., Takagaki, Y., Kanagawa, O. J. Immunol. (1989) [Pubmed]
WikiGenes - Universities