Disease relevance of IGKV1-22

• Epstein-Barr virus (EBV) DNA polymerase was released from phorbol ester-treated tamarin (Saguinus oedipus) cells (B95-8) and prepared for use as an antigen by sequential column chromatography with DEAE-Sephadex A-25, DEAE-cellulose, phosphocellulose, and single-stranded DNA cellulose [1].
• Possible dual function of M protein: resistance to bacteriophage A25 and resistance to phagocytosis by human leukocytes [2].
• Growth effects of tyrphostins A25 and AG1478 on colorectal tumor cells were studied to explore therapeutic potential [3].
• Lack of association between the HLA-A10 (A25), B18 and C2o haplotype and anaphylactoid purpura (AP) [4].
• Fusion protein from solubilized E. coli cells was purified to homogeneity by ion exchange chromatography on DEAE-, CM-cellulose, QAE Sephadex A25 columns and selective precipitation [5].

High impact information on IGKV1-22

• Since labeled A25 increases only after the rapidly transported proteins reach regions of nerve containing regenerating axons, we conclude that it most likely arises from post-translational modification of a transported protein [6].
• After exclusion from mini-columns of SP-Sephadex C25 and DEAE-Sephadex A25, two neutral peptides were obtained in homogeneous form by high performance liquid chromatography with an HCl/methanol gradient [7].
• The -1 frameshift-stimulating mRNA pseudoknot from pea enation mosaic virus-1 (PEMV-1) is composed nearly entirely of RNA triple helix.(4) The 2'-OH hydroxyl protons of riboses C15 and C16 are hydrogen bond donors to the N1 atoms of adenosines A27 and A25, respectively, positioned in the minor groove of pseudoknot stem S1 [8].
• The cAMP potentiated iNOS activity was inhibited by the tyrosine kinase inhibitor, A25 (10-200 microM) and the Janus activated kinase 2 inhibitor, B42 (10-200 microM) [9].
• Further analysis demonstrated selective pathology in layer V of A25, POF and AI that would disrupt direct cortico-autonomic projections [10].

Chemical compound and disease context of IGKV1-22

• CASE SUMMARY: A 25-year-old white man developed a rash while receiving piperacillin/tazobactam 3.375 g intravenously every 6 hours and gentamicin for osteomyelitis complicating a left femur fracture secondary to a motorcycle accident [11].

Biological context of IGKV1-22

• A heterozygous C2 deficiency (C2D) was found in only one of the seven kindreds studied, and followed an A25, B18 DR7 haplotype [12].
• For residue A25 in the active site, the power dependence of T(1)(rho) yielded an exchange lifetime similar to that previously measured by line-shape analysis, and provides an important calibration of this T(1)(rho) methodology for probing the dynamics of macromolecules [13].
• The D5-PL internal bulge nucleotides stack into the helical junction, resulting in a coupling between the bulge A25 and the closing base pair (G8-C27) of the lower helix [14].
• Induction of apoptosis was more efficient with AG1478 than with A25 (tenfold in VACO235) [3].
• The results were discussed with the help of known HLA amino acid sequences, and the positions of the following epitopes were suggested: A2/Aw69; A2/28; A2/11/25/26/28/29/30/31/Aw33/34; A25/32; Bw4; B7/Bw22,42; Bw6 [15].

Anatomical context of IGKV1-22

• A25 is rearranged in 50% of the FDCP-mix cell lines and M4 in 10% [16].
• N-terminally extended forms of 5-oxoprolylglutamylprolinamide (Glp-Glu-Pro-NH2), a thyrotropin-releasing-hormone(TRH)-like peptide associated with the male reproductive system, were isolated from human semen by gel exclusion on Sephadex G50, ion-exchange chromatography on SP-Sephade C25 and QAE-Sephadex A25, and by HPLC [17].
• RESULTS: A 25-year-old woman developed bilateral vision loss with pain on eye movement in association with disk edema, dilated retinal veins, and hemorrhage [18].
• Injection of 16 microg of venom protein into sixth instar larvae was sufficient to reduce the ability of hemocytes to encapsulate Sephadex A25 beads by more than 50% in four of five insects examined [19].
• When stained by A25 etc, coarse granular antigen or inclusion bodies were found without demonstrable Ig and Clq [20].

Associations of IGKV1-22 with chemical compounds

• Pretreatment with VO(4)(-3) antagonized genistein-induced augmentation and A23- or A25-induced suppression of I(Cl.vol) [21].
• In contrast to the stimulatory effects of genistein, 100 microM tyrphostin A23 (AG 18) and A25 (AG 82) inhibited I(Cl.vol) by 38.2 +/- 4.9% and 40.9 +/- 3.4%, respectively [21].
• The column was made up of QAE-Sephadex (A25, formate form) packed to a height of 4 cm in Pasteur pipets [22].
• No association between low C2 levels and the HLA haplotype A10(A25), B18 or an isolated A25 or B18 was observed [4].
• Glycopeptides present in a pronase digest of human Tamm-Horsfall glycoprotein were fractionated by chromatography on DEAE-Sephadex A25 in 0.1M acetic acid [23].

Analytical, diagnostic and therapeutic context of IGKV1-22

• Simultaneous radioimmunoassay for plasma arginine-vasopressin and oxytocin using DEAE Sephadex A 25 extraction [24].
• After intubation, heart rate increased significantly in the control group, remained at initial levels in the A10 and A25 groups and decreased in the A50 group [25].
• We conclude that a single DEAE-Sephadex A25 purification step is insufficient to remove extraneous proteins and MP, which could interfere with the production of AP-specific antibodies and the dissection of moieties responsible for immune reactivity [26].
• Vp-TDH/I was purified by a series of column chromatographies on DEAE-Sephadex A25, hydroxyapatite, Sepharose 4B and Mono Q [27].
• The final DEAE-Sephadex A25 chromatographic step resulted in (a) removal of detectable extraneous proteins, (b) removal of immunologically detectable MP by dot blot and Western blot enzyme immunoassay, (c) loss of periodic acid-silver stain positivity, and (d) a high AP yield (1295 U l-1) and specific activity (1749 U mg-1) [26].

References