Disease relevance of F10

• Glial fibrillary acidic protein (GFAP) was induced in rat C6 glioma cells grown in M199 and HAM F10 media by addition of 1 mM dibutyryl cyclic AMP [1].
• The role of membrane estrogen receptor-alpha (mERalpha) in rapid nongenomic responses to 17beta-estradiol (E(2)) was tested in sublines of GH3/B6 rat prolactinoma cells selected for high (GH3/B6/F10) and low (GH3/B6/D9) mERalpha expression [2].
• The anti-angiogenic effect of SR 25989 was further assessed in vitro in a quantitative assay of angiogenesis comprising a fragment of rat aorta embedded in a fibrin gel and in vivo in a pulmonary metastatic model using C57BL/6 mice inoculated in the foot pad with the highly metastatic melanoma cell line B16 F10 [3].
• Of the two other radiolabels, [75Se]selenomethionine had the advantage over [3H]proline of higher labelling efficiency (especially in Ham's F10 medium), lower toxicity, and being a gamma-emitter [4].
• When isolated islets were stored in CAB-4 degrees C for 96 h, they yielded slightly higher numbers of dissociated cells than after culture in HAM's F10 at 20 degrees C. Implantation of cold stored islets from 2 donor pancreata corrected hyperglycemia in streptozotocin-diabetic rats [5].

High impact information on F10

• The mitogenic effect of activated coagulation factor X (factor Xa) was examined in cultured aortic smooth muscle cells (VSMC) from Wistar-Kyoto rats (WKY) [6].
• Peptides 69-84-Gly and (P80)68-86, which lacked stimulatory activity toward many encephalitogenic T cells in our proliferation assay when standard APC were used, become strongly stimulatory in the presence of less commonly used APC, i.e., an Ia+ T cell clone (LOA) or an Ia-inducible rat glial cell clone (F10) [7].
• The myocytes were prepared from neonatal rats and cultured in F10 medium that was supplemented with serum [8].
• DX-9065a is an antithrombin III (AT III)-independent and selective inhibitor of activated blood coagulation factor X (FXa) [9].
• (+)-2S-2-[4-[[(3S)-1-Acetimidoyl-3- pyrrolidinyl]oxy]phenyl]-3-[7-amidino-2-naphthyl]propanoic acid hydrochloride pentahydrate (DX-9065a) is an antithrombin III (AT III)-independent and selective inhibitor of activated blood coagulation factor X (FXa) [10].

Biological context of F10

• In studies to understand the regulatory mechanisms involved in Ia expression, a glial cell clone generated from the brains of neonatal Lewis rats (F10 clone) readily expressed class II major histocompatibility (Ia) antigens after stimulation by interferon-gamma (IFN-gamma) at doses from 10 to 100 units/ml [11].
• Two of the three groups were submitted to two different levels of hypoxia (FiO2 = 0.05, group F5 and FiO2 = 0.1, group F10) and the third to normoxia (FiO2 = 0.21, group F21) in a thermoneutral and controlled environment [12].
• More than 50% of the eighth to tenth generation (F8-F10) offsprings of the Px-rats showed regular 4-day estrous cycles at 15-16 months of age, while nearly 80% of normal F8-F10 rats developed persistent estrus at 13-14 months of age [13].
• PCA was evaluated by measurement of the level of activation of coagulation factor X using specific chromogenic substrate S-2222 [14].

Anatomical context of F10

• Examination of both local and propagating seizure profiles of the new strains from F6 to F10 revealed that the FAST and SLOW rats had similar amygdala afterdischarge (AD) thresholds and associated AD durations [15].
• When the growth medium (Ham's F10) was supplemented with rat median eminence extract (MEE), L-thyroxin and fresh serum from hypophysectomized rats, some of the 2A8 cells accumulated and stored secretory granules which were characteristic of the cells of the intact pituitary gland [16].
• Vesicle-treated F1 cells revert to their original arrest behavior and metastatic capacity following removal of F10 vesicle components from the plasma membrane [17].
• Seminiferous tubules were cultured during fifteen days in HAM F10 medium supplemented with fetal calf serum (FCS) and/or follicle-stimulating hormone (FSH) [18].
• TNF rich supernatants were prepared from macrophages cultured in F10 medium +2% Bovine Serum Albumin (BSA) [19].

Associations of F10 with chemical compounds

• We examined the structural requirements, within a series of cysteinyl derivatives, for oxidation of thiols and of LDL in Hams F10 medium [20].
• The livers were perfused for 3 hours with modified Ham's F10 culture medium supplemented with thyroxine hormone (T4) [21].
• Serum triglyceride in the F30 group was about 2 times higher than in the F10 and F20 groups [22].
• The paw of male Sprague-Dawley rats were injected with 100 microliter of formalin with 2.5% (F2.5), 5% (F5), and 10% (F10) concentrations [23].

Other interactions of F10

• Ham's F10 and F12 media allowed a six fold increase [24].
• Moreover, Ia-inducing effects of IFN-gamma on F10 cells were also greatly enhanced when these cells were preexposured to T cells [11].
• [Ca(2+)](i) elevation was correlated with prolactin (PRL) release in the F10 cell line in as little as 3 min [2].
• We used modified immunocytochemical conditions to quantify a membrane form of estrogen receptor-alpha (mERalpha) in a rat pituitary tumor cell line, GH3/B6/F10 [25].

Analytical, diagnostic and therapeutic context of F10

• These differences in group F5 as well as the maze solution profile (defined by analysis of 15 variables considered) was significantly closer to the control group F21 than the less hypoxied group F10 [12].
• A murine monoclonal antibody (SV-1/F10) was highly specific immunologically for West African E.carinatus venom both by ELISA and immunoblotting [26].
• At the end of the 53-day treatment period, all animals were placed under chloroform anaesthesia; the caudal epididymides were removed, minced and placed in Ham's F10 solution for the evaluation of sperm count and motility [27].
• Synthesis and secretion of blood coagulation factor X was studied during incubations of hepatocytes prepared by perfusion of rat livers with collagenase [28].

References