The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

F10  -  coagulation factor X

Mus musculus

Synonyms: AI194738, Cf10, Coagulation factor X, Stuart factor, fX
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of F10

  • Using the B16/F10 murine melanoma (B16) as a model for CNS tumor, we show that vaccination with bone marrow-generated DCs, pulsed with either B16 cell extract or B16 total RNA, can induce specific cytotoxic T lymphocytes against B16 tumor cells [1].
  • The mean and variance of the numbers of resultant B16 F1 and B16 F10 melanoma metastases strongly correlated with the power function (r2 greater than 0.8) [2].
  • A strong correlation was found between the basal levels of membrane-bound protein kinase C and the ability of B16 melanoma cell sublines (F10, F1, and BL6) to metastasize to the lung after intravenous injection [3].
  • DFMO administration did not inhibit the experimental metastases induced as a result of i.v. injection of B16 melanoma (line F10) tumor and Lewis lung carcinoma cells into the tail vein [4].
  • To test the hypothesis that genetic instability correlates with malignant potential, we compared the rate of generation of marker chromosomal abnormalities in clones of B16 F1 and B16 F10 murine melanoma [5].

High impact information on F10

  • The exponent b was 1.4 +/- 0.1 and 1.6 +/- 0.2 for the F1 and F10 melanomas, respectively, indicating a clustering of metastases within certain mice [2].
  • By treating with tumor-promoting phorbol esters for 1 hr, the low-metastasizing F1 cells exhibited both translocation of protein kinase C from cytosol to plasma membrane and an increase in metastasis to a level comparable to the (untreated) highly metastatic subline F10 [3].
  • F10 vesicle-modified F1 cells reverted to their original arrest behavior and metastatic capacity after removal of F10 vesicle components from the plasma membrane [6].
  • The therapeutic efficacy of free ET-18-OCH3 and ELL-12 was investigated against i.p. P388 leukemia, Lewis lung cancer lung metastases, and B16/F10 melanoma (lung tumor nodules) in mice [7].
  • B16 sublines selected for various organ colonization properties differentially expressed gp90, bound 125I-labeled transferrin, and responded differently to purified transferrin in proliferation assays in relation to their metastatic properties (B15b greater than O13 greater than F10 greater than F1) [8].

Chemical compound and disease context of F10


Biological context of F10

  • The cDNA encoding murine coagulation factor X (fX) was isolated and reconstructed from a lambdaZap cDNA library generated from murine liver mRNA [14].
  • All protein domains of human and rat fX are strictly conserved in mouse fX [14].
  • By contrast, Cf10, Col4a1, Col4a2, and D8H13S35 mapped near the centromere of mouse Chr 8, defining a new conserved linkage [15].
  • The F10 clones possessed an additional copy of chromosome 1 and also a significantly higher prevalence of the translocation t(9,12) when compared with the F1 clones [5].
  • The highly metastatic F10 line showed the same degree of marker chromosomal instability as the poorly metastatic F1 line (0.01 variants/cell/generation) [5].

Anatomical context of F10

  • Analysis of the metastatic properties of clones isolated from mouse B16 melanoma cell lines (B16-F1 and F10) shows extensive cellular heterogeneity and the presence of subpopulations that have widely differing metastatic abilities [16].
  • Light, scanning, and transmission electron microscopic studies of tumor cell-lymphocyte interaction suggested that the resistance of B16-F10Lr cells to destruction by syngeneic lymphocytes in vitro was due to the masking or absence of tumor-specific antigen(s) present on the lymphocyte-susceptible F10 cells [17].
  • The Ig kappa L chain synthesized by the mouse hybridoma line F10 forms large fibrils in the lumen of the endoplasmic reticulum [18].
  • The efficiency of receptor-mediated entry of pseudotyped virus carrying the surface protein (SU) of clone A8, a neuropathogenic variant of Friend murine leukemia virus (FrMLV), to rat glial cell line F10 was 1 order of magnitude greater than that of pseudotyped virus carrying SU of nonneuropathogenic FrMLV clone 57 [19].
  • Preincubation with 50 micrograms/ml Ac-LDL in F10 medium had no effect on LDL oxidation by either LDLR-/- or C57B6 macrophages [20].

Associations of F10 with chemical compounds

  • Although the five cysteine residues of the aberrant kappa-chain are in the normal positions, they display an unusual gel pattern when the intrachain disulfide bonds are opened with 2-ME; that is, the intrachain disulfide bonding pattern of F10 kappa-chain seems to be unusual [18].
  • The conflicting results between studies of intact vs. broken cell preparations could not be explained by increased cyclic AMP phosphodiesterase activity in F5 and F10 cells [21].
  • Upon loading with the antimalarial drug chloroquine, the MAb F10-bearing liposomes effectively controlled not only the chloroquine-susceptible but also the chloroquine-resistant P. berghei infections in mice [22].
  • Part of the core aldehydes accumulated within the cells.Our study demonstrates that i) J774 macrophages are able to promote/accelerate core aldehyde formation in HAM's F10 medium, and ii) that core aldehyde formation rates can be increased by stimulation of the cells with PMA, and, although to a lesser extent, with LPS [23].
  • The suitability of liposomes as drug carriers in the treatment of drug-resistant rodent malaria was examined after covalently attaching F(ab')2 fragments of a mouse monoclonal antibody (MAb), MAb F10, raised against the host cell membranes isolated from the Plasmodium berghei-infected mouse erythrocytes, to the liposome surface [22].

Regulatory relationships of F10

  • The p53 in F10 cells appears to be as functional as that in NIH3T3 cells because irradiation-induced expression of p53-target genes was comparable in both cells [24].
  • Proto-oncogene c-fos was found to be highly expressed in F10 lines by an in situ hybridization technique and also in F10 lung metastatic nests by immunofluorescent staining with anti-c-fos antibody [25].
  • Inhibition of recombinant interferon-gamma-induced Ia antigen expression by shed B16 F10 melanoma cell membrane vesicles [26].
  • We have examined this by comparing its cytotoxic effects in B16/F10 and B16/F10-differential deficient (-DD) mouse melanoma cells that express high and low levels of tyrosinase activity respectively [27].

Other interactions of F10

  • In this study, the authors examined the efficacy of treating central nervous system (CNS) tumors by transfecting poorly immunogenic B16/F10 melanoma cells with interleukin (IL)-2, IL-4, or granulocytemacrophage-colony stimulating factor (GM-CSF) gene, and using these cells to deliver the cytokine locally at the site of the CNS tumor [28].
  • The possible relationship between GSH and the ability of Bcl-2 to prevent cell death was studied in B16M cells with high (F10) and low (F1) metastatic potential [29].
  • It induced fibronectin expression but not E-cadherin expression in B 16/F10 cells [30].
  • BL6 cells differ from F10 cells by an alteration of the gene encoding the B56gamma regulatory subunit of protein phosphatase 2A (PP2A), which results in mRNA encoding a truncated variant of the subunit (deltagamma1) [24].
  • Production of GM3 in F10 melanomas treated with IL-2 for 4 days increased, and, if the treatment was continued for 7 days, minor components of gangliosides, such as GM2, GM1, and GD1a, appeared only in F1 melanomas, while the increase of production of GM3 disappeared in both melanomas [25].

Analytical, diagnostic and therapeutic context of F10


  1. Bone marrow-generated dendritic cells pulsed with tumor extracts or tumor RNA induce antitumor immunity against central nervous system tumors. Ashley, D.M., Faiola, B., Nair, S., Hale, L.P., Bigner, D.D., Gilboa, E. J. Exp. Med. (1997) [Pubmed]
  2. Experimental metastasis: a novel application of the variance-to-mean power function. Kendal, W.S., Frost, P. J. Natl. Cancer Inst. (1987) [Pubmed]
  3. Tumor promoter-induced membrane-bound protein kinase C regulates hematogenous metastasis. Gopalakrishna, R., Barsky, S.H. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  4. Antimetastatic activity of DL-alpha-difluoromethylornithine, an inhibitor of polyamine biosynthesis, in mice. Sunkara, P.S., Rosenberger, A.L. Cancer Res. (1987) [Pubmed]
  5. Rate of generation of major karyotypic abnormalities in relationship to the metastatic potential of B16 murine melanoma. Kendal, W.S., Wang, R.Y., Hsu, T.C., Frost, P. Cancer Res. (1987) [Pubmed]
  6. Arrest and metastasis of blood-borne tumor cells are modified by fusion of plasma membrane vesicles from highly metastatic cells. Poste, G., Nicolson, G.L. Proc. Natl. Acad. Sci. U.S.A. (1980) [Pubmed]
  7. Enhanced therapeutic effects of liposome-associated 1-O-octadecyl-2-O-methyl-sn-glycero-3-phosphocholine. Ahmad, I., Filep, J.J., Franklin, J.C., Janoff, A.S., Masters, G.R., Pattassery, J., Peters, A., Schupsky, J.J., Zha, Y., Mayhew, E. Cancer Res. (1997) [Pubmed]
  8. Differential expression of a Mr approximately 90,000 cell surface transferrin receptor-related glycoprotein on murine B16 metastatic melanoma sublines selected for enhanced brain or ovary colonization. Nicolson, G.L., Inoue, T., Van Pelt, C.S., Cavanaugh, P.G. Cancer Res. (1990) [Pubmed]
  9. Enhancement of the anticancer activity of bis(2-chloroethyl)nitrosourea in mice by coadministration of 2'-deoxyuridine, 2'-deoxycytidine, or thymidine. Lin, T.S., Prusoff, W.H. Cancer Res. (1987) [Pubmed]
  10. Immunomodulation and enhancement of antitumor activity by co-administration of 1,3-bis(2-chloroethyl)-1-nitrosourea and thymidine. Poo, W.J., Guo, X., Haslund, B., Mozdziesz, D.E. Biochem. Pharmacol. (1997) [Pubmed]
  11. Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3',5'-monophosphate and tiazofurin. Korićanac, L.B., Todorović, D.V., Popović, N.M., Demajo, M.A., Ruzdijić, S.D., Ristić-Fira, A.M. Ann. N. Y. Acad. Sci. (2004) [Pubmed]
  12. Inhibition of tumor cell invasion in the Boyden chamber assay by a mannosidase inhibitor, mannostatin A. Ochi, Y., Atsumi, S., Aoyagi, T., Umezawa, K. Anticancer Res. (1993) [Pubmed]
  13. Effect of cyclophosphamide and levan treatment on bone marrow and peripheral blood cells in B16-F10 melanoma-bearing mice. Leibovici, J., Siegal, A., Kopel, S., Davidai, G., Yavetz, H. Int. J. Immunopharmacol. (1989) [Pubmed]
  14. Cloning and characterization of a cDNA encoding murine coagulation factor X. Liang, Z., Cooper, A., DeFord, M.E., Carmeliet, P., Collen, D., Castellino, F.J., Rosen, E.D. Thromb. Haemost. (1998) [Pubmed]
  15. Homologs of genes and anonymous loci on human chromosome 13 map to mouse chromosomes 8 and 14. Koizumi, T., Hendel, E., Lalley, P.A., Tchetgen, M.B., Nadeau, J.H. Mamm. Genome (1995) [Pubmed]
  16. Interactions among clonal subpopulations affect stability of the metastatic phenotype in polyclonal populations of B16 melanoma cells. Poste, G., Doll, J., Fidler, I.J. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]
  17. Mechanism of tumor cell resistance to lysis by syngeneic lymphocytes. Fidler, I.J., Bucana, C. Cancer Res. (1977) [Pubmed]
  18. Large cytoplasmic inclusion body kappa-chain has unusual intrachain disulfide bonding. Jäck, H.M., Sloan, B., Grisham, G., Reason, D., Wabl, M. J. Immunol. (1993) [Pubmed]
  19. Contribution of virus-receptor interaction to distinct viral proliferation of neuropathogenic and nonneuropathogenic murine leukemia viruses in rat glial cells. Takase-Yoden, S., Watanabe, R. J. Virol. (1999) [Pubmed]
  20. Macrophage oxidative modification of low density lipoprotein occurs independently of its binding to the low density lipoprotein receptor. Tangirala, R.K., Mol, M.J., Steinberg, D. J. Lipid Res. (1996) [Pubmed]
  21. Hormonal activation of adenylate cyclase in mouse melanoma metastatic variants. Niles, R.M., Makarski, J.S. J. Cell. Physiol. (1978) [Pubmed]
  22. Chloroquine encapsulated in malaria-infected erythrocyte-specific antibody-bearing liposomes effectively controls chloroquine-resistant Plasmodium berghei infections in mice. Owais, M., Varshney, G.C., Choudhury, A., Chandra, S., Gupta, C.M. Antimicrob. Agents Chemother. (1995) [Pubmed]
  23. Macrophage-enhanced formation of cholesteryl ester-core aldehydes during oxidation of low density lipoprotein. Karten, B., Boechzelt, H., Abuja, P.M., Mittelbach, M., Sattler, W. J. Lipid Res. (1999) [Pubmed]
  24. A truncated isoform of the PP2A B56gamma regulatory subunit reduces irradiation-induced Mdm2 phosphorylation and could contribute to metastatic melanoma cell radioresistance. Koma, Y.I., Ito, A., Watabe, K., Kimura, S.H., Kitamura, Y. Histol. Histopathol. (2004) [Pubmed]
  25. Differential cell- and immuno-biological properties of murine B16-F1 and F10 melanomas: oncogene c-fos expression, sensitivity to LAK cells and/or IL-2, and components of gangliosides. Nakayama, J., Urabe, K., Tsuchida, T., Urabe, A., Terao, H., Taniguchi, S., Hori, Y. J. Dermatol. (1995) [Pubmed]
  26. Inhibition of recombinant interferon-gamma-induced Ia antigen expression by shed B16 F10 melanoma cell membrane vesicles. Poutsiaka, D.D., Taylor, D.D., Levy, E.M., Black, P.H. J. Immunol. (1985) [Pubmed]
  27. Activation of tyrosinase reduces the cytotoxic effects of the superoxide anion in B16 mouse melanoma cells. Valverde, P., Manning, P., McNeil, C.J., Thody, A.J. Pigment Cell Res. (1996) [Pubmed]
  28. Comparative analysis of paracrine immunotherapy in experimental brain tumors. Lesniak, M.S., Sampath, P., DiMeco, F., Viglione, M.P., Tyler, B.M., Pardoll, D.M., Brem, H. Neurosurgical focus [electronic resource]. (2000) [Pubmed]
  29. Down-regulation of glutathione and Bcl-2 synthesis in mouse B16 melanoma cells avoids their survival during interaction with the vascular endothelium. Ortega, A., Ferrer, P., Carretero, J., Obrador, E., Asensi, M., Pellicer, J.A., Estrela, J.M. J. Biol. Chem. (2003) [Pubmed]
  30. Inhibition of cellular chemotactic invasion by a vinca alkaloid, conophylline. Amino, N., Ohse, T., Koyano, T., Umezawa, K. Anticancer Res. (1996) [Pubmed]
  31. A truncated isoform of the PP2A B56 subunit promotes cell motility through paxillin phosphorylation. Ito, A., Kataoka, T.R., Watanabe, M., Nishiyama, K., Mazaki, Y., Sabe, H., Kitamura, Y., Nojima, H. EMBO J. (2000) [Pubmed]
  32. A unique tumor rejection antigen from the S91 murine malignant melanoma. Law, L.W., Vieira, W.D., Kameyama, K., Hearing, V.J. Cancer Res. (1987) [Pubmed]
  33. Definition of a 1.06-Mb region linked to neuroinflammation in humans, rats and mice. Ockinger, J., Serrano-Fernández, P., Möller, S., Ibrahim, S.M., Olsson, T., Jagodic, M. Genetics (2006) [Pubmed]
  34. Resistance to intravenous tumor metastases in the athymic nude mouse: a paradoxic response. Richie, J.P., McDonald, J., Gittes, R.F. Surgery (1981) [Pubmed]
WikiGenes - Universities