Disease relevance of F12

• Transitional epithelial morphology of normal bladder was well maintained in Waymouth's Medium MB 752/1, while Ham's Medium F12 caused marked epithelial hyperplasia [1].
• Survival and myelination in dorsal root ganglion culture were observed using 6 kinds of modified commercial serum-free media: MEM, neMEM, alpha-MEM, DMEM, F12 medium and DF medium (see Materials and Methods) [2].
• Rat glioma C6 cells were grown in T25 flasks in 5 mL of DMEM/F12 were exposed to Compound A, and the viability of cells was determined at various time points by trypan blue exclusion [3].

High impact information on F12

• METHODS: After dissociation of the liver parenchyma by collagenase perfusion, the liver remnant containing the intact biliary tree was minced into small fragments, embedded in a rat tail collagen gel, and cultured for 6 days in hormonally defined serum-free Dulbecco's Modified Eagle Medium/F12 medium (SFDM) [4].
• Thirty-eight urinary bladders from Fischer rats were organ cultured for 7 days in Ham's Medium F12 with varying concentrations of hydrocortisone added [5].
• Urinary bladders from Fischer rats organ cultured in a chemically defined medium, Ham's F12, underwent transitional cell hyperplasia which persisted for the duration of the culture period (10 days) [6].
• The combination of hydrocortisone and insulin markedly inhibited the proliferative response of normal bladder epithelium grown in Medium F12 [1].
• It consisted of a 1:1 mixture of Ham's F12 and DME media with the addition of insulin, transferrin, triiodothyronine, prolactin, growth hormone, and an extract of proteose peptone (medium IM) [7].

Biological context of F12

• The tubules were seeded into culture dishes, and cell growth was monitored in both Dulbecco's Modified Eagle's Medium containing 10% fetal calf serum and in a defined medium consisting of 50:50 Ham's F12 and Dulbecco's supplemented with insulin, transferrin, and hydrocortisone [8].
• The material was conclusively identified as PGI2 by identification of its hydrolysis product 6-oxo-prostaglandin F12 (6-oxo-PGF1 alpha) by gas chromatography-mass spectrometry (GC-MS) [9].
• These explants were cultured in DMEM/F12 medium treated or not with 50 ng/ml of corticosterone; after 22 hr, vincristine was added to the medium for 2 hr to block mitosis [10].
• An LD50 value of 1 mM was calculated when these cells were exposed to paraquat in vitro for 12 h in Ham's F12 culture medium at 30 degrees C. Cell death was accompanied by the formation of TBA-reactive substances (lipid peroxidation) and was potentiated by hyperoxia (95% O2) [11].
• Cell viability, as assessed by trypan blue exclusion, was significantly greater at all time points tested when hepatocytes were cultured in Ham's F12 medium containing 132 microM O2, as compared to medium equilibrated with air (220 microM O2) or air + oxygen (298 microM O2) [12].

Anatomical context of F12

• Separated thyroid follicles are stable in suspension culture in Coon's modified Ham's F12 medium containing 0.5% calf serum [13].
• Glial cells grown in serum-free, chemically defined medium (F12 basal medium supplemented with putrescine, selenium, insulin, transferrin, and BSA) replicated their DNA to a limited extent even in the absence of serum mitogens [14].
• The addition of essential amino acids to the DMEM/F12 medium enhanced the DNA synthesis of hepatocytes, thus indicating that the higher level of amino acids in L-15 medium may be an important factor in its enhanced ability to support the proliferation of primary cultured rat hepatocytes [15].
• METHODS: The rat gastric mucosal cell line RGM-1 was grown in DMEM/F12 medium supplemented with 10% FCS [16].
• NPCs from E12.5 rat ventral mesencephalon were cultured as neurospheres in DMEM/F12 medium containing N2 supplements and bFGF [17].

Associations of F12 with chemical compounds

• Substitution of phenylalanine at position 25 to alanine (A12), had little effect on the inhibition of different Ca(2+)/CaM-dependent protein kinases, suggesting that phenylalanine 25 does not play a crucial role in the interactions involving F12 [18].
• The cultures were kept for the initial seven days in a Coon's modified F12 medium, then were switched to a medium containing ascorbic acid and beta-glycerophosphate (BGP), and cultured for another 41 days [19].
• High survival was achieved with osmolarity lower than found in Dulbecco's Modified Eagle's Medium (DMEM), and by reducing cysteine and glutamine concentrations and by the elimination of toxic ferrous sulphate found in DME/F12 [20].
• For example, in Dulbecco's modified Eagle medium/F12 medium (containing 5.5 mmol/L glucose), the peak ratio of oxidation to reduction (with 1 denoting equivalence of oxidative and reductive activities) was 5.5 in rat Leydig cells, 19.7 in COS1 cells, and 20.8 in CHOP cells [21].
• DiI labelled motoneurons could be maintained in culture on poly-L-lysine coating and Dulbecco Minimum Essential Medium with Ham F12 complement, supplemented with additives and 3% fetal calf serum [22].

Other interactions of F12

• Ham's F10 and F12 media allowed a six fold increase [23].
• The cultured rat astrocytes were incubated with 1 microM of double-labelled transferrin (125I-Tf-59Fe) in serum- free DMEM F12 medium or 59Fe II in isotonic sucrose solution at 37 degrees C or 4 degrees C for varying times [24].
• This defined medium consists of 1:1 (vol/vol) mixture of Ham's F12 and Dulbecco's modified Eagle's media supplemented with insulin, dibutyryl cyclic AMP, hydrocortisone, epidermal growth factor, selenium, and albumin/linoleic acid complex [25].
• Adult rat testes were placed in fresh oxygenated DMEM F12 medium, decapsulated, and the tubules gently teased apart [26].
• The model of rat primary hepatocytes incubated in DMEM/F12 (Ham) medium was used for studying the influence of the cAMP-effectors epinephrine (100 microM), norepinephrine (100 microM), glucagon (1 microM) and isoproterenol (1-1000 microM) as well as the synthetic cAMP-analogon dibutyryl-cAMP on the metabolism of metallothionein [27].

Analytical, diagnostic and therapeutic context of F12

• Dialysates of heat-inactivated epididymal extracts were fractionated by liquid chromatography, and 4 fractions-F2, F5, F7, and F12-were found to contain endogenous inhibitors of PDE [28].

References