The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Links

 

Gene Review

IL10  -  interleukin 10

Canis lupus familiaris

 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of IL10

 

High impact information on IL10

 

Biological context of IL10

  • Canine IL-10 exhibits strong sequence homology to the known sequences of human, mouse, rat, and bovine genes at nucleotide and amino acid levels [6].
  • Canine cytokine mRNA expression in peripheral blood mononuclear cells collected prior to treatment and at the completion of the study was determined for IFN-gamma, IL-4, TNF and IL-10 genes using quantitative reverse transcription competitive polymerase chain reaction [7].
 

Anatomical context of IL10

  • The IL10 significantly limited lymph DC cluster formation with T cells [8].
  • There were no significant changes in symptom scores; lymphocyte proliferation; secreted IFN-gamma, IL-10, or IL-5; or intracellular cytokine production [9].
  • In particular, we measured the amount of parasites in samples such as blood, lymph nodes and skin, and the expression levels of IFN-gamma, IL-2, IL-4, IL-10, IL-12 and IL-18 cytokines in the blood [10].
  • Control and gluten-sensitive IS showed similar total and grade-by-grade areas of expression of IFNgamma and IL10 in the lamina propria (p>0.05), in contrast to beagles, which showed greater total and grade 1 areas of expression of IFNgamma, and greater total, grade 1 and grade 2 areas of expression of IL10, than both groups of IS (p<0.05) [11].
  • We describe here the cloning of canine IL-10 cDNA (GenBank accession No. U33843) and the expression of recombinant IL-10 in a dog kidney cell line (DK6247) and Chinese hamster ovary cells (CHO) [6].
 

Associations of IL10 with chemical compounds

  • Summarized, TNF and IL-6, associated with disease exacerbation, and IL-10 and TGF, indicative of remission, were often observed simultaneously in distemper DL and could not be assigned to a specific disease stage [12].
  • Duodenal IL-10 mRNA levels decreased (p = 0.1) and colonic IFN-gamma mRNA levels increased (p = 0.08) after probiotic treatment [13].
 

Other interactions of IL10

  • Furthermore, the expression of interleukin- (IL-2), IL-12(p40), tumour necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), IL-10 and transforming growth factor-beta (TGF-beta) as well as inducible nitric oxid synthase (iNOS) mRNA was determined by reverse transcription-polymerase chain reaction (RT-PCR) [14].
  • In the present study, an RT-PCR method has been standardised and employed to evaluate the frequency and the semi-quantitative level of expression of the cytokines IL-4, IL-10, IL-12, INF-gamma and TNF-alpha in splenocytes of 30 dogs naturally infected with Leishmania chagasi and of 7 non-infected dogs (NID) [15].
  • Dogs with patent Dirofilaria immitis infection have higher expression of circulating IL-4, IL-10 and iNOS mRNA than those with occult infection [16].
  • Relative RT-PCR results, expressed as %GAPDH, revealed a significant up-regulation of IL-6, -8, -12 and TNF-alpha mRNA in distemper dogs; whereas IL-10 and TGF-beta showed only a weak and not significantly increased expression following infection [17].
 

Analytical, diagnostic and therapeutic context of IL10

  • This study has validated the use of in situ hybridization for the detection of IFNgamma and IL10 mRNA within canine intestinal biopsies, andhas shown heightened jejunal expression of both cytokines in beagles with high enteric bacterial populations [11].
  • In the absence of reperfusion, no significant induction of IL-10 mRNA was noted [3].
  • Oral administration of specific antigens to allergy-prone infant dogs induces IL-10 and TGF-beta expression and prevents allergy in adult life [18].
  • Analysis of cytokine mRNA level suggested that vaccinated dogs had elevated IFN-gamma mRNA in peripheral blood mononuclear cells (PBMC), whereas there was a consistent increase in the level of IL-10 in the control groups and some vaccinated dogs [19].
  • The humoral immune response displayed in these animals, the cell-mediated immunosuppression, nor the disease severity could be related with the expression of IL-10 [20].

References

  1. Quantitative analysis of inflammatory and immune responses in dogs with gastritis and their relationship to Helicobacter spp. infection. Wiinberg, B., Spohr, A., Dietz, H.H., Egelund, T., Greiter-Wilke, A., McDonough, S.P., Olsen, J., Priestnall, S., Chang, Y.F., Simpson, K.W. J. Vet. Intern. Med. (2005) [Pubmed]
  2. Expression of cytokine mRNA in canine anal furunculosis lesions. House, A., Gregory, S.P., Catchpole, B. Vet. Rec. (2003) [Pubmed]
  3. IL-10 is induced in the reperfused myocardium and may modulate the reaction to injury. Frangogiannis, N.G., Mendoza, L.H., Lindsey, M.L., Ballantyne, C.M., Michael, L.H., Smith, C.W., Entman, M.L. J. Immunol. (2000) [Pubmed]
  4. IL-19 induces production of IL-6 and TNF-alpha and results in cell apoptosis through TNF-alpha. Liao, Y.C., Liang, W.G., Chen, F.W., Hsu, J.H., Yang, J.J., Chang, M.S. J. Immunol. (2002) [Pubmed]
  5. Bioartificial kidney ameliorates gram-negative bacteria-induced septic shock in uremic animals. Fissell, W.H., Lou, L., Abrishami, S., Buffington, D.A., Humes, H.D. J. Am. Soc. Nephrol. (2003) [Pubmed]
  6. Cloning and expression of canine interleukin-10. Lu, P., Zucker, K., Fuller, L., Tzakis, A., Esquenazi, V., Miller, J. J. Interferon Cytokine Res. (1995) [Pubmed]
  7. Use of immunostimulatory liposome-nucleic acid complexes in allergen-specific immunotherapy of dogs with refractory atopic dermatitis - a pilot study. Mueller, R.S., Veir, J., Fieseler, K.V., Dow, S.W. Vet. Dermatol. (2005) [Pubmed]
  8. Cytokines and adherence molecules involved in spontaneous dendritic cell-lymphocyte clustering in skin afferent lymph. Galkowska, H., Wojewodzka, U., Olszewski, W.L. Scand. J. Immunol. (1995) [Pubmed]
  9. Immunologic response to administration of standardized dog allergen extract at differing doses. Lent, A.M., Harbeck, R., Strand, M., Sills, M., Schmidt, K., Efaw, B., Lebo, T., Nelson, H.S. J. Allergy Clin. Immunol. (2006) [Pubmed]
  10. Leishmania DNA load and cytokine expression levels in asymptomatic naturally infected dogs. Manna, L., Reale, S., Viola, E., Vitale, F., Manzillo, V.F., Michele, P.L., Caracappa, S., Gravino, A.E. Vet. Parasitol. (2006) [Pubmed]
  11. In situ hybridization as a technique for the immunological investigation of canine intestine: jejunal expression of IFNgamma and IL10 in Irish setters and beagles. Garden, O.A., Elwood, C.M., Desport, M., Batt, R.M. Vet. Immunol. Immunopathol. (1999) [Pubmed]
  12. Dominating interleukin-10 mRNA expression induction in cerebrospinal fluid cells of dogs with natural canine distemper virus induced demyelinating and non-demyelinating CNS lesions. Frisk, A.L., Baumgärtner, W., Gröne, A. J. Neuroimmunol. (1999) [Pubmed]
  13. Effects of probiotic bacteria in dogs with food responsive diarrhoea treated with an elimination diet. Sauter, S.N., Benyacoub, J., Allenspach, K., Gaschen, F., Ontsouka, E., Reuteler, G., Cavadini, C., Knorr, R., Blum, J.W. Journal of animal physiology and animal nutrition. (2006) [Pubmed]
  14. The regression of a canine Langerhans cell tumour is associated with increased expression of IL-2, TNF-alpha, IFN-gamma and iNOS mRNA. Kaim, U., Moritz, A., Failing, K., Baumgärtner, W. Immunology (2006) [Pubmed]
  15. Analysis of the cytokine profile in spleen cells from dogs naturally infected by Leishmania chagasi. Lage, R.S., Oliveira, G.C., Busek, S.U., Guerra, L.L., Giunchetti, R.C., Corr??a-Oliveira, R., Reis, A.B. Vet. Immunol. Immunopathol. (2007) [Pubmed]
  16. Dogs with patent Dirofilaria immitis infection have higher expression of circulating IL-4, IL-10 and iNOS mRNA than those with occult infection. Morch??n, R., L??pez-Belmonte, J., Bazzocchi, C., Grandi, G., Kramer, L., Sim??n, F. Vet. Immunol. Immunopathol. (2007) [Pubmed]
  17. Increased expression of pro-inflammatory cytokines and lack of up-regulation of anti-inflammatory cytokines in early distemper CNS lesions. Markus, S., Failing, K., Baumgärtner, W. J. Neuroimmunol. (2002) [Pubmed]
  18. Oral administration of specific antigens to allergy-prone infant dogs induces IL-10 and TGF-beta expression and prevents allergy in adult life. Zemann, B., Schwaerzler, C., Griot-Wenk, M., Nefzger, M., Mayer, P., Schneider, H., de Weck, A., Carballido, J.M., Liehl, E. J. Allergy Clin. Immunol. (2003) [Pubmed]
  19. Protective vaccination against experimental canine visceral leishmaniasis using a combination of DNA and protein immunization with cysteine proteinases type I and II of L. infantum. Rafati, S., Nakhaee, A., Taheri, T., Taslimi, Y., Darabi, H., Eravani, D., Sanos, S., Kaye, P., Taghikhani, M., Jamshidi, S., Rad, M.A. Vaccine (2005) [Pubmed]
  20. Cytokine expression during the outcome of canine experimental infection by Leishmania infantum. Santos-Gomes, G.M., Rosa, R., Leandro, C., Cortes, S., Romão, P., Silveira, H. Vet. Immunol. Immunopathol. (2002) [Pubmed]
 
WikiGenes - Universities