The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Links

 

Gene Review

DCLRE1C  -  DNA cross-link repair 1C

Gallus gallus

Synonyms: SNM1C, artemis, nuclease
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of DCLRE1C

  • We previously showed that these components, when bound with histones on plasmids containing the region, confer on the complex a pattern of hypersensitivity to nuclease digestion similar to that in the nucleus [1].
  • The transcriptionally active ev-3 and inactive ev-1 endogenous retrovirus loci in chick cells differ in that ev-3 is undermethylated, preferentially sensitive to DNase I digestion, and contains nuclease hypersensitive sites in each of its two long terminal repeats [2].
  • In this cell type, the ev-3 preintegration site is organized in a nuclease-resistant conformation, while the ev-3 provirus is in a nuclease-sensitive conformation and is transcribed [3].
  • On the basis of the nuclease S1 protection assay with uniformly labeled single-stranded pRLC429 DNA, subcloned into M13mp18 phage vector, we conclude that the rat atrial muscle also contains MLC2 of the ventricular type [4].
  • Both 4 S RNA and the 10 S RNP are potent inhibitors of translation of a variety of mRNAs such as chick muscle poly(A)+ mRNA, rabbit globin mRNA, EMC virus RNA, and poly(A)- and mRNA of rat liver in micrococcal nuclease-treated rabbit reticulocyte lysate [5].
 

High impact information on DCLRE1C

  • These fragments do not result from bulk nucleosome phasing in vivo, but arise from micrococcal nuclease cleavages internal to the core particle, at roughly 10-base pair intervals and at AT-rich sequences [6].
  • We believe that some aspect of DNA sequence is translated into an altered DNA structure in chromatin and that it is this altered structure that is recognized by s1 nuclease and possibly by certain chromosomal proteins [7].
  • Using nuclease digestion, one can progressively cleave DNA from the loops, thereby isolating residual DNA that is progressively closer to the nuclear matrix anchorage sites [8].
  • Furthermore, a 115 base pair DNA fragment contained within this nuclease-sensitive region can be excised by Msp I digestion and released from nuclei, in at least 50% yield [9].
  • A 200 base pair region at the 5' end of the chicken adult beta-globin gene is accessible to nuclease digestion [9].
 

Chemical compound and disease context of DCLRE1C

  • Analysis of nuclease hypersensitivity of regions flanking the estrogen-dependent, chicken apoVLDLII gene has revealed an hepatic, DNaseI hypersensitive site whose sensitivity is influenced by both the developmental stage and sex of the bird [10].
 

Biological context of DCLRE1C

  • Genetic analyses confirm this suggestion as we have demonstrated an epistatic relationship between 53BP1 and the NHEJ genes, Ku70 and Artemis, but not with Rad54, a gene essential for repair of DSBs by homologous recombination [11].
  • Furthermore, in contrast to the uninsulated reporter genes, chromatin over the insulated genes retains nuclease accessibility and histone hyperacetylation [12].
  • Nuclease protection experiments on intact nuclei show that at early stages of embryonic development, the CACCC site is occupied and the Pal site is vacant, but as development progresses, the Pal site is filled gradually and the CACCC site loses its bound protein [13].
  • RNA interference is an evolutionarily conserved gene-silencing pathway in which the nuclease Dicer cleaves double-stranded RNA into small interfering RNAs [14].
  • Alternatively, the scaffold DNA was prepared from micrococcal nuclease-digested intact chromosomes using sucrose gradients containing 2M NaCl [15].
 

Anatomical context of DCLRE1C

  • The structure of chicken erythrocyte and chicken liver chromatin has been studied by enzymatic digestion with micrococcal nuclease and DNAase I [16].
  • This suggests that active genes are preferentially digested by deoxyribonuclease I. In contrast, treatment of red cell nuclei with staphylococcal nuclease results in no preferential digestion of active globin genes [17].
  • Fragments of 20-25, 40-50, 90-110, and 160-180 base pairs (bp), along with intermediate-size pieces were isolated by preparative gel electrophoresis of a limited micrococcal nuclease digest of calf thymus DNA [18].
  • Nuclei of chicken oviduct cells and of erythrocytes, and preparations of 'naked' DNA were digested with DNase I and with micrococcal nuclease [19].
  • Correct and efficient transient expression directed by the lysozyme promoter was found in both of these cell lines, as determined by S1 nuclease mapping and Northern blot analysis of the RNAs made [20].
 

Associations of DCLRE1C with chemical compounds

  • SV40 DNA replicated in the presence of cycloheximide was more sensitive to staphylococcal nuclease digestion and had a lower superhelical density than viral DNA replicated in the absence of this drug [21].
  • In contrast to this, over 75% of the 5-methylcytosine was protected from nuclease digestion by chromatin proteins [22].
  • Of particular interest is the presence in 50S hnRNP of a nuclease-resistant region (24-28 nucleotides long) in both IVS immediately upstream from the putative lariat branch site in an RNA splicing intermediate [23].
  • A region of about 200 base pairs 5' to the chicken beta A-globin gene, which contains sites sensitive to nuclease S1, to several restriction endonucleases, and to very low levels of DNase I, also contains DNA structures that are preferentially sensitive to bromoacetaldehyde [24].
  • This observation argues against the possibility that the generation of nucleosomal DNA following drug treatment of nuclei is due to an activation of endogenous nuclease by bleomycin and strongly suggests that the drug has a unique feature of action on chromatin [25].
 

Physical interactions of DCLRE1C

  • In this paper, electrophoretic mobility shift assays (EMSAs) and S1 nuclease treatment are used to demonstrate that the RBF-maltose binding protei (MBP) fusion protein binds to single-stranded DNA of its element [26].
 

Enzymatic interactions of DCLRE1C

  • Rather, an analysis of the fragmentation of the ovalbumin chromatin as a function of digestion extent suggested a mechanism in which the heightened sensitivity resulted from the collective expansion of the nuclease cutting sites in the linker regions of the ovalbumin chromatin because the gene was in an unfolded conformation [27].
 

Other interactions of DCLRE1C

  • 53BP1 contributes to survival of cells irradiated with X-ray during G1 without Ku70 or Artemis [28].
  • Using the techniques of runoff transcription, primer extension, and S1 nuclease protection, we demonstrate that there is a third c-myc exon of approximately equal to 345 base pairs (bp) located 0.7 kbp upstream of the 5' end of the v-myc homology [29].
  • We studied the developmental and tissue-specific expression of phospholamban mRNA using nuclease mapping analysis [30].
  • We also present evidence for extensive, differentiation-dependent alterations in nuclease accessibility at the lysozyme promoter without alterations of nucleosome and transcription factor occupancy [31].
  • We have precisely determined the positions of the first three exons for the major chicken vitellogenin gene (VTG II) by a combination of S1 nuclease protection, primer extension and DNA sequencing experiments [32].
 

Analytical, diagnostic and therapeutic context of DCLRE1C

References

  1. Interaction of specific nuclear factors with the nuclease-hypersensitive region of the chicken adult beta-globin gene: nature of the binding domain. Emerson, B.M., Lewis, C.D., Felsenfeld, G. Cell (1985) [Pubmed]
  2. Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation. Groudine, M., Eisenman, R., Weintraub, H. Nature (1981) [Pubmed]
  3. Varied interactions between proviruses and adjacent host chromatin. Conklin, K.F., Groudine, M. Mol. Cell. Biol. (1986) [Pubmed]
  4. Heart myosin light chain 2 gene. Nucleotide sequence of full length cDNA and expression in normal and hypertensive rat. Kumar, C.C., Cribbs, L., Delaney, P., Chien, K.R., Siddiqui, M.A. J. Biol. Chem. (1986) [Pubmed]
  5. A ribonuclease-resistant cytoplasmic 10 S ribonucleoprotein of chick embryonic muscle. A potent inhibitor of cell-free protein synthesis. Sarkar, S., Mukherjee, A.K., Guha, C. J. Biol. Chem. (1981) [Pubmed]
  6. Another potential artifact in the study of nucleosome phasing by chromatin digestion with micrococcal nuclease. McGhee, J.D., Felsenfeld, G. Cell (1983) [Pubmed]
  7. An altered DNA conformation detected by S1 nuclease occurs at specific regions in active chick globin chromatin. Larsen, A., Weintraub, H. Cell (1982) [Pubmed]
  8. The ovalbumin gene is associated with the nuclear matrix of chicken oviduct cells. Robinson, S.I., Nelkin, B.D., Vogelstein, B. Cell (1982) [Pubmed]
  9. A 200 base pair region at the 5' end of the chicken adult beta-globin gene is accessible to nuclease digestion. McGhee, J.D., Wood, W.I., Dolan, M., Engel, J.D., Felsenfeld, G. Cell (1981) [Pubmed]
  10. Organization, sequence and nuclease hypersensitivity of repetitive elements flanking the chicken apoVLDLII gene: extended sequence similarity to elements flanking the chicken vitellogenin gene. Haché, R.J., Deeley, R.G. Nucleic Acids Res. (1988) [Pubmed]
  11. Genetic dissection of vertebrate 53BP1: a major role in non-homologous end joining of DNA double strand breaks. Nakamura, K., Sakai, W., Kawamoto, T., Bree, R.T., Lowndes, N.F., Takeda, S., Taniguchi, Y. DNA Repair (Amst.) (2006) [Pubmed]
  12. Loss of transcriptional activity of a transgene is accompanied by DNA methylation and histone deacetylation and is prevented by insulators. Pikaart, M.J., Recillas-Targa, F., Felsenfeld, G. Genes Dev. (1998) [Pubmed]
  13. Developmental modulation of protein binding to beta-globin gene regulatory sites within chicken erythrocyte nuclei. Jackson, P.D., Evans, T., Nickol, J.M., Felsenfeld, G. Genes Dev. (1989) [Pubmed]
  14. Dicer is essential for formation of the heterochromatin structure in vertebrate cells. Fukagawa, T., Nogami, M., Yoshikawa, M., Ikeno, M., Okazaki, T., Takami, Y., Nakayama, T., Oshimura, M. Nat. Cell Biol. (2004) [Pubmed]
  15. Analysis of DNA attached to the chromosome scaffold. Kuo, M.T. J. Cell Biol. (1982) [Pubmed]
  16. A comparison of the structure of chicken erythrocyte and chicken liver chromatin. Morris, N.R. Cell (1976) [Pubmed]
  17. Chromosomal subunits in active genes have an altered conformation. Weintraub, H., Groudine, M. Science (1976) [Pubmed]
  18. Reaction of systemic lupus erythematosus antinative DNA antibodies with native DNA fragments from 20 to 1,200 base pairs. Papalian, M., Lafer, E., Wong, R., Stollar, B.D. J. Clin. Invest. (1980) [Pubmed]
  19. A close association between sites of DNase I hypersensitivity and sites of enhanced cleavage by micrococcal nuclease in the 5'-flanking region of the actively transcribed ovalbumin gene. Kaye, J.S., Bellard, M., Dretzen, G., Bellard, F., Chambon, P. EMBO J. (1984) [Pubmed]
  20. Transient expression of the chicken lysozyme gene after transfer into human cells. Matthias, P.D., Renkawitz, R., Grez, M., Schütz, G. EMBO J. (1982) [Pubmed]
  21. The asymmetric segregation of parental nucleosomes during chrosome replication. Seidman, M.M., Levine, A.J., Weintraub, H. Cell (1979) [Pubmed]
  22. Distribution of 5-methylcytosine in chromatin. Razin, A., Cedar, H. Proc. Natl. Acad. Sci. U.S.A. (1977) [Pubmed]
  23. Specific regions of the intervening sequences of beta-globin RNA are resistant to nuclease in 50S heterogeneous nuclear RNA-protein complexes. Patton, J.R., Chae, C.B. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  24. Detection of an altered DNA conformation at specific sites in chromatin and supercoiled DNA. Kohwi-Shigematsu, T., Gelinas, R., Weintraub, H. Proc. Natl. Acad. Sci. U.S.A. (1983) [Pubmed]
  25. Altered gel electrophoretic mobility of bleomycin-mediated release of nucleosomal DNA. Kuo, M.T. Cancer Res. (1981) [Pubmed]
  26. Interactions of the nuclear matrix-associated steroid receptor binding factor with its DNA binding element in the c-myc gene promoter. Barrett, T.J., Sandhu, N.P., Tomlinson, A.J., Benson, L.M., Subramaniam, M., Naylor, S., Spelsberg, T.C. Biochemistry (2000) [Pubmed]
  27. Hormonal regulation of the conformation of the ovalbumin gene in chick oviduct chromatin. Bloom, K.S., Anderson, J.N. J. Biol. Chem. (1982) [Pubmed]
  28. 53BP1 contributes to survival of cells irradiated with X-ray during G1 without Ku70 or Artemis. Iwabuchi, K., Hashimoto, M., Matsui, T., Kurihara, T., Shimizu, H., Adachi, N., Ishiai, M., Yamamoto, K., Tauchi, H., Takata, M., Koyama, H., Date, T. Genes Cells (2006) [Pubmed]
  29. Transcription of three c-myc exons is enhanced in chicken bursal lymphoma cell lines. Linial, M., Groudine, M. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  30. Characterization of cDNA and genomic sequences encoding a chicken phospholamban. Toyofuku, T., Zak, R. J. Biol. Chem. (1991) [Pubmed]
  31. Differentiation-dependent alterations in histone methylation and chromatin architecture at the inducible chicken lysozyme gene. Lefevre, P., Lacroix, C., Tagoh, H., Hoogenkamp, M., Melnik, S., Ingram, R., Bonifer, C. J. Biol. Chem. (2005) [Pubmed]
  32. Identification and sequence analysis of the 5' end of the major chicken vitellogenin gene. Burch, J.B. Nucleic Acids Res. (1984) [Pubmed]
  33. Termination of the ovalbumin gene transcription. LeMeur, M.A., Galliot, B., Gerlinger, P. EMBO J. (1984) [Pubmed]
  34. Differences of supranucleosomal organization in different kinds of chromatin: cell type-specific globular subunits containing different numbers of nucleosomes. Zentgraf, H., Franke, W.W. J. Cell Biol. (1984) [Pubmed]
  35. Nuclease resistance and the enrichment of native nuclear acceptor sites for the avian oviduct progesterone receptor. Hora, J., Horton, M.J., Toft, D.O., Spelsberg, T.C. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  36. In vitro core particle and nucleosome assembly at physiological ionic strength. Ruiz-Carrillo, A., Jorcano, J.L., Eder, G., Lurz, R. Proc. Natl. Acad. Sci. U.S.A. (1979) [Pubmed]
  37. Linker DNA accessibility in chromatin fibers of different conformations: a reevaluation. Zlatanova, J., Leuba, S.H., Yang, G., Bustamante, C., van Holde, K. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
 
WikiGenes - Universities