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Gene Review

THBS1  -  thrombospondin 1

Sus scrofa

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Disease relevance of THBS1


High impact information on THBS1

  • In contrast, exposure of SMCs to vitronectin (1.0 micrograms/cm2) or thrombospondin (0.25 micrograms/cm2), two known ligands for alphaVbeta3, resulted in enhancement of the IGF-I-stimulated IRS-1 response [3].
  • Interaction of thrombospondin-1 and heparan sulfate from endothelial cells. Structural requirements of heparan sulfate [4].
  • Cell surface-associated heparan sulfate proteoglycans, predominantly perlecan, are involved in the process of binding and endocytosis of thrombospondin-1 (TSP-1) by vascular endothelial cells [4].
  • Further depolymerization of the binding oligosaccharides revealed that the glucosamine residues within the TSP-1-binding sequences are not continuously N-sulfated [4].
  • The present findings implicate specific structural properties for the HS domain involved in TSP-1 binding and indicate that they are distinct from the binding sequence described for basic fibroblast growth factor, another HS ligand and a potential antagonist of TSP-1 [4].

Biological context of THBS1

  • In the presence of thrombospondin-1 cells did not organize in follicle-like structures but, in contrast, spreaded and reached confluency independently of cell proliferation [5].
  • The addition of 1.0 microg/ml of this mutant did not inhibit the protein synthesis or cell migration responses to IGF-I plus TS-1 [6].
  • Our data suggest that the control of thyroid follicle formation may operate at least in part through regulation of the production of the matricellular protein TSP1, which acts as a negative modulator of the cell-cell adhesion process involved in thyroid follicle morphogenesis [7].
  • Thrombospondin and osteopontin bind to insulin-like growth factor (IGF)-binding protein-5 leading to an alteration in IGF-I-stimulated cell growth [8].
  • Given that there are three binding sites per molecule and that thrombospondin appears to form polymeric aggregates with itself, significant binding energies could be developed [9].

Anatomical context of THBS1


Associations of THBS1 with chemical compounds


Physical interactions of THBS1


Other interactions of THBS1


Analytical, diagnostic and therapeutic context of THBS1

  • Affinity chromatography on immobilized TSP-1 demonstrated that nearly all intact HS chains possessed binding affinity, whereas after heparinase III treatment only a small proportion of oligosaccharides were bound with similar affinity to the column [4].
  • Further, immunofluorescence showed cellular codistribution of thrombospondin and thyroglobulin, although thrombospondin exhibited predominantly an extracellular, basolateral deposition [10].
  • By using western blotting with a monoclonal antibody against the calcium-binding domain of thrombospondin, the authors detected a 180-kD glycated polypeptide in the trabecular meshwork tissue of normal human and porcine eyes [1].
  • We used antisera directed against human platelet thrombospondin (TSP) and microfibril-associated GP 128 to localize the presence of these glycoproteins in fixed sections of human placenta or porcine arteries and skin by immunogold labeling, using electron microscopy [18].


  1. Synthesis of a thrombospondin-like cytoadhesion molecule by cells of the trabecular meshwork. Tripathi, B.J., Tripathi, R.C., Yang, C., Millard, C.B., Dixit, V.M. Invest. Ophthalmol. Vis. Sci. (1991) [Pubmed]
  2. Responsive site on the thrombospondin-1 promotor to down-regulation by phorbol 12-myristate 13-acetate in porcine aortic endothelial cells. Kim, S.A., Hong, K.J. Exp. Mol. Med. (2000) [Pubmed]
  3. Blocking ligand occupancy of the alphaVbeta3 integrin inhibits insulin-like growth factor I signaling in vascular smooth muscle cells. Zheng, B., Clemmons, D.R. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  4. Interaction of thrombospondin-1 and heparan sulfate from endothelial cells. Structural requirements of heparan sulfate. Feitsma, K., Hausser, H., Robenek, H., Kresse, H., Vischer, P. J. Biol. Chem. (2000) [Pubmed]
  5. Influence of transforming growth factor beta1 (TGF-beta1) on the behaviour of porcine thyroid epithelial cells in primary culture through thrombospondin-1 synthesis. Claisse, D., Martiny, I., Chaqour, B., Wegrowski, Y., Petitfrere, E., Schneider, C., Haye, B., Bellon, G. J. Cell. Sci. (1999) [Pubmed]
  6. Insulin-like growth factor binding protein-5 (IGFBP-5) interacts with thrombospondin-1 to induce negative regulatory effects on IGF-I actions. Moralez, A.M., Maile, L.A., Clarke, J., Busby, W.H., Clemmons, D.R. J. Cell. Physiol. (2005) [Pubmed]
  7. Regulation of the three-dimensional organization of thyroid epithelial cells into follicle structures by the matricellular protein, thrombospondin-1. Pellerin, S., Croizet, K., Rabilloud, R., Feige, J.J., Rousset, B. Endocrinology (1999) [Pubmed]
  8. Thrombospondin and osteopontin bind to insulin-like growth factor (IGF)-binding protein-5 leading to an alteration in IGF-I-stimulated cell growth. Nam, T.J., Busby, W.H., Rees, C., Clemmons, D.R. Endocrinology (2000) [Pubmed]
  9. Binding properties of human thrombospondin: interaction with mucopolysaccharides. Slayter, H.S., Karp, G., Miller, B.E., Rosenberg, R.D. Semin. Thromb. Hemost. (1987) [Pubmed]
  10. Polarized secretion of thrombospondin is opposite to thyroglobulin in thyroid epithelial cells. Prabakaran, D., Kim, P., Kim, K.R., Arvan, P. J. Biol. Chem. (1993) [Pubmed]
  11. Differential expression of thrombospondin, collagen, and thyroglobulin by thyroid-stimulating hormone and tumor-promoting phorbol ester in cultured porcine thyroid cells. Bellon, G., Chaqour, B., Antonicelli, F., Wegrowski, J., Claisse, D., Haye, B., Borel, J.P. J. Cell. Physiol. (1994) [Pubmed]
  12. Lysophosphatidic acid activates nuclear factor kappa B and induces proinflammatory gene expression in endothelial cells. Palmetshofer, A., Robson, S.C., Nehls, V. Thromb. Haemost. (1999) [Pubmed]
  13. Binding and endocytosis of thrombospondin and thrombospondin fragments in endothelial cell cultures analyzed by cuprolinic blue staining, colloidal gold labeling, and silver enhancement techniques. Völker, W., Schön, P., Vischer, P. J. Histochem. Cytochem. (1991) [Pubmed]
  14. Integrin-associated protein binding domain of thrombospondin-1 enhances insulin-like growth factor-I receptor signaling in vascular smooth muscle cells. Maile, L.A., Clemmons, D.R. Circ. Res. (2003) [Pubmed]
  15. Down-regulated expression of A disintegrin and metalloproteinase with thrombospondin-like repeats-1 by progesterone receptor antagonist is associated with impaired expansion of porcine cumulus-oocyte complexes. Shimada, M., Nishibori, M., Yamashita, Y., Ito, J., Mori, T., Richards, J.S. Endocrinology (2004) [Pubmed]
  16. Different action of heparin and fucoidan on arterial smooth muscle cell proliferation and thrombospondin and fibronectin metabolism. Vischer, P., Buddecke, E. Eur. J. Cell Biol. (1991) [Pubmed]
  17. Dietary-induced atherosclerotic lesions have increased levels of acidic FGF mRNA and altered cytoskeletal and extracellular matrix mRNA expression. Liau, G., Winkles, J.A., Cannon, M.S., Kuo, L., Chilian, W.M. J. Vasc. Res. (1993) [Pubmed]
  18. Thrombospondin: a component of microfibrils in various tissues. Arbeille, B.B., Fauvel-Lafeve, F.M., Lemesle, M.B., Tenza, D., Legrand, Y.J. J. Histochem. Cytochem. (1991) [Pubmed]
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