Disease relevance of DMAP1

• A recombinant enzyme expressed in Escherichia coli catalyzed isopentenyl transfer reaction from dimethylallyldiphosphate (DMAPP) to the N6 amino group of adenosine monophosphate (AMP), adenosine diphosphate (ADP) and adenosine triphosphate (ATP), respectively [1].
• Contrast of response to dacarbazine, mitomycin, doxorubicin, and cisplatin (DMAP) plus GM-CSF between patients with advanced malignant gastrointestinal stromal tumors and patients with other advanced leiomyosarcomas [2].

Psychiatry related information on DMAP1

• When GPP was utilized as allylic substrate, adult prenyltransferase displayed cooperative behavior; while with DMAPP, biphasic kinetics were observed [3].
• A total of 72 cyclization reactions were carried out to study the factors that influence head-to-tail cyclization: linear precursor sequence, coupling reagent, residue configuration, the proportion of DMAP additive, concentration, reaction temperature and reaction time [4].

High impact information on DMAP1

• DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci [5].
• DMAP1 has intrinsic transcription repressive activity, and binds to the transcriptional co-repressor TSG101 [5].
• We have developed "planar-chiral" derivatives of 4-(dimethylamino)pyridine (DMAP), a highly versatile nucleophilic catalyst, that are effective in a diverse array of processes, including the Staudinger synthesis of beta-lactams, the acylation of silyl ketene acetals, and the kinetic resolution of amines [6].
• This repeat is in the 5' untranslated region of the gene DIP2B, which encodes a protein with a DMAP1-binding domain, which suggests a role in DNA methylation machinery [7].
• Using a yeast two-hybrid method, we isolated a putative corepressor, DNA methyltransferase 1-associating protein (DMAP1), which was found to bind to the CC domain of RMP [8].

Biological context of DMAP1

• Although the p33ING1-Sin3-HDAC and DMAP1-DNMT1 complexes are recruited independently to pericentric heterochromatin regions, they are both required for deacetylation of histones and methylation of histone H3 at lysine 9 [9].
• Bmi1 cooperates with Dnmt1-associated protein 1 in gene silencing [10].
• Loss of Dmap1 binding to the Bmi1 target loci was tightly associated with derepressed gene expression in Bmi1(-/-) cells [10].
• Key to the preparation of the pentadepsipeptide 26 incorporating the backbone ester was the asymmetric synthesis of an orthogonally protected l-threo-beta-hydroxyasparagine and the development of effective and near-racemization free conditions for esterification of its hindered alcohol (EDCI, DMAP, 0 degrees C) [11].
• The reaction mechanism has also been studied experimentally in dichloromethane through analysis of the reaction kinetics for the acetylation of cyclohexanol with acetic anhydride, in the presence of DMAP as catalyst and triethylamine as the auxiliary base [12].

Anatomical context of DMAP1

• RESULTS: Significantly high levels of GrA were found in the serum and supernatants of lymphocytes from patients with active Behçet's disease cultured in the presence of DMAPP [13].
• Enzyme derived from corpora allata homogenates of both the larval and adult stage of M. sexta coupled each of the DMAPP analogs to produce homologous geranyl and farnesyl diphosphate products in the order (Z)-3-ethyl>(Z)-3-n-propyl>(Z)-3-methyl (DMAPP)>(Z)-3-i-propyl(Z)-3-n-butyl [14].
• Isopentenyl diphosphate (IPP), dimethylallyl diphosphate (DMAPP) and some structural analogs of (E)-1-hydroxy-2-methyl-but-2-enyl 4-diphosphate also stimulated Vgamma9/Vdelta2 T-cell proliferation, albeit at much higher concentrations [15].

Associations of DMAP1 with chemical compounds

• The Km for DMAPP was high at 8 mM, and the kcat of 1.7 s-1 was low, but similar to those of other allylic diphosphate-utilizing enzymes [16].
• An enzyme extracted from aspen (Populus tremuloides) leaves was previously found to catalyze the Mg(2+)-dependent elimination of pyrophosphate from dimethylallyl diphosphate (DMAPP) to form isoprene (Silver, G. M., and Fall, R. (1991) Plant Physiol. 97, 1588-1591) [16].
• The DMAP unit was shown to regioselectively relay 1,1'-carbonyldiimidazole (but not phosgene) to the proximal amino group, as designed, but rotation of the triptycene does not occur [17].
• Carbon dioxide inserts reversibly into the Al-OR bond to give the compound (TPP)AlO(2)COR, and this reaction is promoted by the addition of DMAP [18].
• Ureas 1 and 7 were prepared by reacting 2-aminopyridine and aminonaphthyridine 25, respectively, with triphosgene and 4-(dimethylamino)pyridine (DMAP) [19].

Physical interactions of DMAP1

• We undertook a yeast two-hybrid screen for nuclear RGS6-binding proteins and here identify DMAP1 as an RGS6-interacting protein [20].
• Both Daxx and DMAP1 formed a complex with DNMT1 and colocalized in the nucleus [21].

Enzymatic interactions of DMAP1

• Molecular modeling shows that the modified IDI2 is still likely to catalyze the isomerization of IPP to DMAPP [22].

Regulatory relationships of DMAP1

• RGS6 inhibited the transcriptional repressor activity of DMAP1 [20].

Other interactions of DMAP1

• Gbeta5 and DMAP1 did not compete for each other's interaction with RGS6 [20].
• Collectively, our findings suggest that Bmi1 incorporates Dmap1 in polycomb gene silencing [10].
• This review covers the biosynthesis of the starter units of terpenoids, isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) via the nonmevalonate pathway together with a new enzyme involved in the conversion of IPP and DMAPP, i.e type 2 IPP isomerase [23].

Analytical, diagnostic and therapeutic context of DMAP1

• [reaction: see text] The combination of the ionic liquid [bmim]PF(6) and DMAP provides a most simple and practical approach to the immobilization of OsO(4) as catalyst for olefin dihydroxylation [24].
• The barriers to enantiomerization of a series of axially chiral biaryl analogues of 4-(dimethylamino)pyridine (DMAP) 1-10 were determined experimentally by means of dynamic HPLC measurements and racemization studies [25].
• The adsorption of DMAP onto gold surfaces was studied by electrochemistry and surface plasmon resonance [26].
• The solubility and stability of the DMAP-protected gold nanoparticles were studied as a function of pH using UV-visible spectroscopy and transmission electron microscopy (TEM) [26].

References