Disease relevance of Polarography

• The relationship between the anticonvulsant properties of SC-13504 and its plasma levels, measured by polarography, in baboons with photosensitive epilepsy [1].
• Determination of metallothionein content in hepatoma cells by differential pulse polarography [2].
• CGRP activity was evaluated by measuring local blood flow in neuromas using hydrogen polarography and laser-Doppler flowmetry [3].

High impact information on Polarography

• Molecular weight criteria and differential pulse polarography behavior suggest that one of the species is a metallothionein-like protein [4].
• The reaction of nitrite ion with ascorbic acid and its effect on the rate of nitrosation of secondary amines have been investigated by differential pulse polarography in aqueous acidic solution [5].
• The Sr(2+)- and Ca(2+)-containing enzymes were compared using EPR spectroscopy, UV-visible absorption spectroscopy, and O2 polarography [6].
• We examined local nerve blood flow proximal and distal to sciatic nerve transection in rats with long-term (8 month) experimental streptozotocin diabetes using laser Doppler flowmetry and microelectrode hydrogen clearance polarography [7].
• Polarography experiments were also conducted with the electrode placed in the aqueous subphase to measure the binding of factor Va to pure PS monolayers [8].

Biological context of Polarography

• The effects of chloracyzine (2-chloro-10-(3-diethylaminopropionyl)-phenothiazine hydrochloride; antianginal drug) on myocardial oxygen consumption were studied in open-chest cats and cat isolated hearts using oximetry and polarography respectively [9].
• Nitrosourea concentrations were assayed in plasma and tissues by differential pulse polarography so as to assess whether the pharmacokinetics could explain the differences in antitumor activity [10].
• Mitochondrial respiratory function (state 2, 3, and 4), respiratory control index (RCI), rate of oxidative phosphorylation (ADP/Delta t), and ADP:O ratio were measured by polarography with the use of NADH- or FADH-dependent substrates [11].
• Lipid peroxidation was examined by oxygen polarography, gas-liquid chromatography (GLC) and the thiobarbituric acid test [12].
• Hydrolysis of cloxacillin at pH 4.0 yields an electroactive product which can be determined by polarography [13].

Anatomical context of Polarography

• Phase sensitive alternating current polarography was introduced for the simultaneous determination of p-nitroanisole and its metabolites p-nitrophenol and p-nitrocatechol in kinetic studies with rat liver microsomes [14].
• Oxidation of glutamate + malate in the mitochondria estimated by polarography was significantly inhibited by MPP+, and synthesis of ATP was inhibited to approximately 9% of that of control by 0.06 mM of MPP+ [15].
• Liver mitochondria were isolated and the respiration was determined by polarography using glutamate and succinate as substrates [16].

Associations of Polarography with chemical compounds

• Determination of nitrite ion using differential pulse polarography [17].
• Differential pulse polarography was used to monitor the titration of crab (Cancer pagurus) cadmium metallothionein with additional cadmium [18].
• Determination of chloroacetaldehyde in air by differential pulse polarography [19].
• Determination of vanillylmandelic acid in urine by use of catalytic maximum wave in differential pulse polarography [20].
• We show that polarography can be a useful tool for the recognition of the mode of binding of pyridoxal 5'-phosphate to proteins [21].

Gene context of Polarography

• The optimum conditions using DC polarography and the determination of doxazosin employing SIAP and SCAP polarographic techniques are described in this study [22].
• Comparison of the radiochemical, polarographic, spectrophotometric, and coupled assays for MAO B from beef liver (mitochondria and homogeneous enzyme) showed polarography to be the method of choice on all substrates tested, although, if all known sources of error are carefully controlled, the radiochemical assay gives the same Vmax and Km values [23].
• High-performance pulse and differential pulse polarography. Part I. Theoretical considerations [24].
• Simultaneous determination of superoxide dismutase and catalase in biological materials by polarography [25].
• Polarographic study of aflatoxins B1, B2, G1 and G2: application of differential-pulse polarography to the determination of aflatoxin B1 in various foodstuffs [26].

Analytical, diagnostic and therapeutic context of Polarography

• To determine the oxidation state of technetium in these agents, we studied the reduction of pertechnetate (Tc-99) in 0.1 M solutions of four hydroxycarboxylic acids, using polarography and amperometric titration with Sn(II) [27].
• Raman spectroscopy quantifies copper-adenine complex formation and H(2)O(2) consumption; polarography quantifies O(2) production [28].
• Differential pulse polarography and chronoamperometry were used as electrochemical techniques to characterize the AChE biosensors [29].
• The effect of TRH on nitric oxide release from GH3 cells was studied by means of reverse-phase HPLC to measure NO-2 and NO-3 concentrations in the incubation medium, and by polarography using electrodes specific for NO [30].
• The amount of nitrite formed in this reaction (analyzed by capillary electrophoresis) was compared with the amount of oxygen consumed (measured by polarography) [31].

References