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Chemical Compound Review

EIPA     3-amino-6-chloro-N- (diaminomethylidene)-5...

Synonyms: Lopac-A-3085, CHEMBL517986, AG-J-19240, A3085_SIGMA, CHEBI:606573, ...
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Disease relevance of Ethylisopropylamiloride

  • These findings are consistent with short-term exposure to EIPA accelerating ATP depletion during ischemia, as well as limiting proton efflux during reperfusion [1].
  • 6. In CO2-HCO3(-)-buffered Tyrode solution, pHi recovery from an intracellular acidosis (NH4+ pre-pulse) was only partially inhibited by EIPA or amiloride whereas Nao+ removal completely inhibited the recovery [2].
  • OBJECTIVE: The aim was to investigate the effect of ethylisopropylamiloride (EIPA), an inhibitor of Na+/H+ exchange, and of extracellular pH on the proliferation of immortalised lymphoblasts derived from patients with essential hypertension and normotensive controls [3].
  • Addition of 1 x 10(-5) mol/l amiloride or 3 x 10(-7) mol/l 5-(N-ethyl-N-isopropyl)amiloride (EIPA) markedly decreased the incidence and duration of ventricular fibrillation or even suppressed fibrillation completely as in the case of 1 x 10(-6) mol/l EIPA [4].
  • 3. Release is also enhanced by lignocaine but inhibited by amiloride and ethylisopropylamiloride, suggesting that sodium entry into adrenergic nerve terminals during anoxia occurs by Na+/H+ (and possibly Na+/Ca2+) exchange [5].

High impact information on Ethylisopropylamiloride


Chemical compound and disease context of Ethylisopropylamiloride


Biological context of Ethylisopropylamiloride


Anatomical context of Ethylisopropylamiloride


Associations of Ethylisopropylamiloride with other chemical compounds


Gene context of Ethylisopropylamiloride

  • Further, exposure of the cells to GM-CSF, IL-3, or TPA results in sustained pHi alkalinizatio, which is abrogated when the cells are preincubated with 5-(N-ethyl-N-isopropyl)amiloride, a specific inhibitor of the antiport [30].
  • At a maximum noncytotoxic concentration (25 microM), the potent amiloride analog, 5-(N-ethyl-N-isopropyl)amiloride (EP-Am), decreased but did not abolish, the stimulatory effect of hPRL [31].
  • ET-1- but not PMA-stimulated K+ uptake was inhibited by 5-(N-ethyl-N-isopropyl)amiloride (inhibitor of Na+/H+ exchange system), suggesting a linkage of Na+/H+ exchange with ET-1-stimulated Na+,K(+)-ATPase and Na(+)-K(+)-Cl- cotransport activity that is not mediated by PKC [32].
  • Cytosolic pH (pHi) was significantly higher in GLUT1 than LacZ cells, an effect abolished in the presence of Na+/H+ exchange inhibitor ethylisopropylamiloride (1 micromol/L) [33].
  • When the tubules of the WT mice were exposed to the hyperosmotic solution including either of the two P-gp inhibitors, in the absence of peritubular Na+ or in the presence of peritubular ethylisopropylamiloride (EIPA; the specific inhibitor of Na+/H+ exchange), they did not exhibit RVI [34].

Analytical, diagnostic and therapeutic context of Ethylisopropylamiloride


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  17. Regulation of the formation of tumor cell pseudopodia by the Na(+)/H(+) exchanger NHE1. Lagana, A., Vadnais, J., Le, P.U., Nguyen, T.N., Laprade, R., Nabi, I.R., Noël, J. J. Cell. Sci. (2000) [Pubmed]
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  21. Intracellular pH in human arterial smooth muscle. Regulation by Na+/H+ exchange and a novel 5-(N-ethyl-N-isopropyl)amiloride-sensitive Na(+)- and HCO3(-)-dependent mechanism. Neylon, C.B., Little, P.J., Cragoe, E.J., Bobik, A. Circ. Res. (1990) [Pubmed]
  22. Cytoplasmic domain of the ubiquitous Na+/H+ exchanger NHE1 can confer Ca2+ responsiveness to the apical isoform NHE3. Wakabayashi, S., Ikeda, T., Noël, J., Schmitt, B., Orlowski, J., Pouysségur, J., Shigekawa, M. J. Biol. Chem. (1995) [Pubmed]
  23. Novel amiloride analog allosterically modulates the alpha 2-adrenergic receptor but does not inhibit Na+/H+ exchange. Wilson, A.L., Womble, S.W., Prakash, C., Cragoe, E.J., Blair, I.A., Limbird, L.E. Mol. Pharmacol. (1992) [Pubmed]
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  26. Regulation of intracellular pH in human platelets. Effects of thrombin, A23187, and ionomycin and evidence for activation of Na+/H+ exchange and its inhibition by amiloride analogs. Zavoico, G.B., Cragoe, E.J., Feinstein, M.B. J. Biol. Chem. (1986) [Pubmed]
  27. Ca2+ mobilization can occur independent of acceleration of Na+/H+ exchange in thrombin-stimulated human platelets. Zavoico, G.B., Cragoe, E.J. J. Biol. Chem. (1988) [Pubmed]
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  29. Non-genomic regulation of intermediate conductance potassium channels by aldosterone in human colonic crypt cells. Bowley, K.A., Morton, M.J., Hunter, M., Sandle, G.I. Gut (2003) [Pubmed]
  30. Role of protein kinase C and the Na+/H+ antiporter in suppression of apoptosis by granulocyte macrophage colony-stimulating factor and interleukin-3. Rajotte, D., Haddad, P., Haman, A., Cragoe, E.J., Hoang, T. J. Biol. Chem. (1992) [Pubmed]
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  32. Endothelin 1 stimulates Na+,K(+)-ATPase and Na(+)-K(+)-Cl- cotransport through ETA receptors and protein kinase C-dependent pathway in cerebral capillary endothelium. Kawai, N., Yamamoto, T., Yamamoto, H., McCarron, R.M., Spatz, M. J. Neurochem. (1995) [Pubmed]
  33. Regulation of cytosolic pH and lactic acid release in mesangial cells overexpressing GLUT1. Lang, K.S., Mueller, M.M., Tanneur, V., Wallisch, S., Fedorenko, O., Palmada, M., Lang, F., Bröer, S., Heilig, C.W., Schleicher, E., Weigert, C. Kidney Int. (2003) [Pubmed]
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  37. Effects of inhibitors of enzymatic and cellular pH-regulating systems on central sympathetic chemosensitivity. König, S.A., Offner, B., Czachurski, J., Seller, H. Pflugers Arch. (1995) [Pubmed]
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  39. Regulation by PKC isoforms of Na(+)/H(+) exchanger in luminal membrane vesicles isolated from cortical tubules. Karim, Z.G., Chambrey, R., Chalumeau, C., Defontaine, N., Warnock, D.G., Paillard, M., Poggioli, J. Am. J. Physiol. (1999) [Pubmed]
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