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Gene Review

DCTN3  -  dynactin 3 (p22)

Homo sapiens

Synonyms: DCTN-22, DCTN22, Dynactin complex subunit 22 kDa subunit, Dynactin subunit 3, p22
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Disease relevance of DCTN3

  • We examined sequential serum samples from 12 patients with well-characterized posttransfusion non-A, non-B hepatitis who had an acute, resolving self-limited type of clinical course for the presence of antibody to the hepatitis C virus nucleocapsid (core) protein (p22) expressed by a recombinant baculovirus [1].
  • Consistent with the difference in the genome structure, these two sarcoma viral RNA'S yielded distinct major translation products in cell-free systems, I.E., A 50,000-dalton polypeptide (P50) from Ki-MuSV and a 22,000-dalton polypeptide (p22) from Ha-MuSV [2].
  • In the present study, we found that some B. burgdorferi strain 297 genes (e.g., ospA, mlp-7A, mlp-8, p22, and lp6.6) that typically are regulated by temperature or pH displayed their predicted pattern of expression when B. burgdorferi was cultivated in BSKH medium; this was not true when spirochetes were cultivated in conventional BSKII medium [3].
  • A ribosyltransferase from C. botulinum type D ADP-ribosylated a protein of 22 kDa (p22) in human astrocytoma (1321N1) cells [4].
  • In this study we describe the establishment of two hybridoma cell lines secreting human monoclonal antibodies to the 22-kD nucleocapsid protein (core, p22) of the hepatitis C virus (HCV) [5].

Psychiatry related information on DCTN3


High impact information on DCTN3

  • Database searches with the predicted amino acid sequence of p22 indicate that this polypeptide is novel [7].
  • Immunocytochemistry with antibodies to p22 demonstrates that this polypeptide localizes to punctate cytoplasmic structures and to the centrosome during interphase, and to kinetochores and to spindle poles throughout mitosis [7].
  • An antipeptide antibody against p22 residues 153-164 was found to bind only to permeabilized neutrophils, indicating that the mutation occurs in a cytoplasmic domain [8].
  • The most distinctive chromosome alterations shared between tumor colony-forming cells and the HA-A cell line were double-minute bodies (DMs) and a homogeneously staining region (HSR) on chromosome 7 at band p22 [9].
  • These findings indicate that p22 NF-E4 is capable of influencing human globin gene expression in vivo but is incapable of overriding the intrinsic mechanisms governing gamma-gene silencing in this context [10].

Biological context of DCTN3


Anatomical context of DCTN3


Associations of DCTN3 with chemical compounds

  • Immunoblots with ICE specific antibodies and NH2-terminal sequencing indicated that ICE active column fractions contained in addition to p20 and p10 an alternatively processed form of the p20 protein (p22) containing an extra 16 amino acids NH2-terminal to the p20 [13].
  • Calcineurin B homologous protein 1 (CHP1), also known as p22, is a calcium-binding protein that plays a role in membrane trafficking and binds to multiple effector proteins, including Na+/H+ exchangers, serine/threonine protein kinase and calcineurin, potentially modulating their function [16].

Analytical, diagnostic and therapeutic context of DCTN3

  • Affinity chromatography experiments indicate that p22 binds directly to the p150(Glued) subunit of dynactin [7].
  • This new assay detecting the antibody to the p22 protein is thus useful for the serodiagnosis of non-A, non-B hepatitis in the acute phase and for blood screening [1].
  • With mAb raised to p72, p30, and p22, these proteins were shown to share several antigenic determinants when analyzed by immunoblotting [17].
  • Alu-PCR products generated from hybrid lines containing small overlapping fragments from Xp21-p22 were hybridized to an X chromosome cosmid library, and cosmids predicted by their hybridization pattern to map to the region of interest were analyzed by fluorescence in situ hybridization (FISH) [18].
  • In an ELISA for IgG antibodies, 13 (87%) of 15 serum samples had antibodies to recombinant antigens; reactivity to p22, p39, p41-G, OspC, and OspF antigens was frequent [19].


  1. Improved serodiagnosis of non-A, non-B hepatitis by an assay detecting antibody to hepatitis C virus core antigen. Katayama, T., Mazda, T., Kikuchi, S., Harada, S., Matsuura, Y., Chiba, J., Ohba, H., Saito, I., Miyamura, T. Hepatology (1992) [Pubmed]
  2. Comparison of the genomic organization of Kirsten and Harvey sarcoma viruses. Shih, T.Y., Williams, D.R., Weeks, M.O., Maryak, J.M., Vass, W.C., Scolnick, E.M. J. Virol. (1978) [Pubmed]
  3. Influence of cultivation media on genetic regulatory patterns in Borrelia burgdorferi. Yang, X., Popova, T.G., Goldberg, M.S., Norgard, M.V. Infect. Immun. (2001) [Pubmed]
  4. A 22 kDa ras-related G-protein is the substrate for an ADP-ribosyltransferase from Clostridium botulinum. Quilliam, L.A., Brown, J.H., Buss, J.E. FEBS Lett. (1988) [Pubmed]
  5. Isolation and epitope characterization of human monoclonal antibodies to hepatitis C virus core antigen. Siemoneit, K., da Silva Cardoso, M., Wölpl, A., Koerner, K., Subanek, B. Hybridoma (1994) [Pubmed]
  6. A "new" chromosome marker common to the Rett syndrome and infantile autism? The frequency of fragile sites at X p22 in 81 children with infantile autism, childhood psychosis and the Rett syndrome. Gillberg, C., Wahlström, J., Hagberg, B. Brain Dev. (1985) [Pubmed]
  7. Characterization of the p22 subunit of dynactin reveals the localization of cytoplasmic dynein and dynactin to the midbody of dividing cells. Karki, S., LaMonte, B., Holzbaur, E.L. J. Cell Biol. (1998) [Pubmed]
  8. Point mutation in the cytoplasmic domain of the neutrophil p22-phox cytochrome b subunit is associated with a nonfunctional NADPH oxidase and chronic granulomatous disease. Dinauer, M.C., Pierce, E.A., Erickson, R.W., Muhlebach, T.J., Messner, H., Orkin, S.H., Seger, R.A., Curnutte, J.T. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  9. Evidence for selection of homogeneously staining regions in a human melanoma cell line. Trent, J.M., Thompson, F.H., Ludwig, C. Cancer Res. (1984) [Pubmed]
  10. The role of p22 NF-E4 in human globin gene switching. Zhou, W., Zhao, Q., Sutton, R., Cumming, H., Wang, X., Cerruti, L., Hall, M., Wu, R., Cunningham, J.M., Jane, S.M. J. Biol. Chem. (2004) [Pubmed]
  11. Assignment of p22 dynactin light chain (DCTN3) to human chromosome region 9p13 by radiation hybrid mapping. Mills, D.R., Jackson, C.L. Cytogenet. Cell Genet. (2001) [Pubmed]
  12. Structure, organization, and chromosomal mapping of the human macrophage scavenger receptor gene. Emi, M., Asaoka, H., Matsumoto, A., Itakura, H., Kurihara, Y., Wada, Y., Kanamori, H., Yazaki, Y., Takahashi, E., Lepert, M. J. Biol. Chem. (1993) [Pubmed]
  13. Purification and characterization of active human interleukin-1 beta-converting enzyme from THP.1 monocytic cells. Miller, D.K., Ayala, J.M., Egger, L.A., Raju, S.M., Yamin, T.T., Ding, G.J., Gaffney, E.P., Howard, A.D., Palyha, O.C., Rolando, A.M. J. Biol. Chem. (1993) [Pubmed]
  14. Stimulation through the T cell receptor leads to interactions between SHB and several signaling proteins. Welsh, M., Songyang, Z., Frantz, J.D., Trüb, T., Reedquist, K.A., Karlsson, T., Miyazaki, M., Cantley, L.C., Band, H., Shoelson, S.E. Oncogene (1998) [Pubmed]
  15. AT1 receptor agonistic antibodies from preeclamptic patients stimulate NADPH oxidase. Dechend, R., Viedt, C., Müller, D.N., Ugele, B., Brandes, R.P., Wallukat, G., Park, J.K., Janke, J., Barta, P., Theuer, J., Fiebeler, A., Homuth, V., Dietz, R., Haller, H., Kreuzer, J., Luft, F.C. Circulation (2003) [Pubmed]
  16. Crystallization and preliminary X-ray crystallographic analysis of rat calcineurin B homologous protein 1. Naoe, Y., Arita, K., Hashimoto, H., Kanazawa, H., Sato, M., Shimizu, T. Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. (2005) [Pubmed]
  17. Characterization of stage-specific antigens of infective larvae of the filarial parasite Brugia malayi. Lal, R.B., Ottesen, E.A. J. Immunol. (1988) [Pubmed]
  18. Identification of YAC and cosmid clones encompassing the ZFX-POLA region using irradiation hybrid cell lines. Francis, F., Benham, F., See, C.G., Fox, M., Ishikawa-Brush, Y., Monaco, A.P., Weiss, B., Rappold, G., Hamvas, R.M., Lehrach, H. Genomics (1994) [Pubmed]
  19. Serologic diagnosis of Lyme borreliosis by using enzyme-linked immunosorbent assays with recombinant antigens. Magnarelli, L.A., Ijdo, J.W., Padula, S.J., Flavell, R.A., Fikrig, E. J. Clin. Microbiol. (2000) [Pubmed]
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