Disease relevance of Aldoc

• The aldolase C mRNA was distributed mainly in the brain, some in ascites hepatoma and fetal liver [1].
• Aldolase C in cultrued rat glioma [2].
• We show by transient transfection assay and electrophoretic-mobility-shift assay (EMSA) that the binding of transcriptional activators to region D is much more efficient (80% versus 30%) in human neuroblastoma cells (SKNBE) than in the non-neuronal cell line A1251, which contains low levels of aldolase C mRNA [3].
• This plasmid produces active rat aldolase C in the transfected E. coli host cells [4].
• An isolation procedure for rat brain aldolase C has been developed which also permits the isolation of aldolase C from experimental hepatomas [5].

High impact information on Aldoc

• It is located in cerebellar Purkinje cells, and the highest concentrations are seen in ones expressing high levels of zebrin II (aldolase C) [6].
• Novel target sequences for Pax-6 in the brain-specific activating regions of the rat aldolase C gene [7].
• Upstream elements involved in vivo in activation of the brain-specific rat aldolase C gene. Role of binding sites for POU and winged helix proteins [8].
• A protein of molecular mass 40 kDa, which was detected by anti-PLD antibody with overlaying of the purified PLD2, is shown to be aldolase C by peptide-mass fingerprinting with matrix-assisted laser desorption/ionization-time-of flight mass spectrometry (MALDI-TOF-MS) [9].
• The site of phosphorylation was identified as Asp-319 on aldolase C. The equivalent residue on aldolase A, a glutamic residue, was not phosphorylated [10].

Biological context of Aldoc

• The rat aldolase C gene was isolated from a rat genomic DNA library [11].
• A fusion gene comprising the 5' regulatory region of the aldolase C gene was expressed in a CNS-specific manner [12].
• Southern blot analysis indicates that probably a single aldolase C gene exists per haploid genome [13].
• Comparison of the amino acid sequences of rat aldolase C with those for rat aldolase A and B [Joh et al. (1985) Gene 39, 17-24; Tsutsumi et al. (1984) J. Biol. Chem. 259, 14572-14575], which have been determined previously, shows the existence of highly conserved stretches of amino acid among the three isozymic forms throughout their sequences [1].
• A 13-kb fragment of the rat aldolase C gene contains sufficient information for gene expression [14].

Anatomical context of Aldoc

• In agreement with previous reports there is, in rat, a transient period during which all Purkinje cells are positive for aldolase C expression [15].
• Aldolase C is mainly expressed in the central nervous system (CNS) [12].
• Neurons were found to contain about 4-fold more aldolase C mRNA and about twice as much aldolase A mRNA than astroglia [16].
• In the human, in contrast to the other species, the photoreceptor cell layer was strongly aldolase C immunoreactive [17].
• Molecular, topographic, and functional organization of the cerebellar cortex: a study with combined aldolase C and olivocerebellar labeling [18].

Associations of Aldoc with chemical compounds

• The dislocations of the Aldoc mRNA bands did not occur after a single methamphetamine injection and thus repeated injections were necessary to change the distributions of the lateral bands [19].
• We concluded that excess dopamine selectively changes the location of the Aldoc mRNA compartments in the vermis while the SLC1A 6 mRNA stripes could be changed by other inputs and thus the specific transmitter system might change the specific compartment of the cerebellum [19].
• The sequence around Asp-319 of aldolase C has some similarities to those around the histidine residues on ATP-citrate lyase and succinic thiokinase that are phosphorylated by nm23 proteins [10].
• Histidine to aspartate phosphotransferase activity of nm23 proteins: phosphorylation of aldolase C on Asp-319 [10].
• Several of the altered proteins (heat shock proteins 60 and 72, albumin, dihydropyrimidinase related protein-2, aldolase c, and malate dehydrogenase) have previously been connected to schizophrenia [20].

Other interactions of Aldoc

• This result suggests that the brain-specific aldolase C gene could constitute a new target for the transcription factor Pax-6, which is implicated increasingly in neurogenesis [7].
• The cellular localization of aldolase C mRNA was determined in the central nervous system along with aldolase A mRNA by in situ hybridization [11].
• In contrast, aldolase B, aldolase C and albumin mRNAs did not change at all [21].
• NGFI-B mRNA and protein expression were promptly (15 min) increased after 8-Br-cAMP treatment and precedes aldolase C mRNA increase (30 min) [22].

Analytical, diagnostic and therapeutic context of Aldoc

• Molecular cloning and expression of rat aldolase C messenger RNA during development and hepatocarcinogenesis [13].
• Functional dissection of the brain-specific rat aldolase C gene promoter in transgenic mice. Essential role of two GC-rich boxes and an HNF3 binding site [23].
• The expression of aldolase C and aldolase A mRNA was assessed by Northern blot hybridization using RNAs purified from cultured rat and mouse brain neurons and astroglial cells [16].
• Immunoassay showed that defective aldolase C molecules were accumulated [24].

References