The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

Slc1a1  -  solute carrier family 1...

Rattus norvegicus

Synonyms: Eaac1, Eaat3, Excitatory amino acid transporter 3, Excitatory amino-acid carrier 1, REAAC1, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Slc1a1


High impact information on Slc1a1

  • Modulation of the neuronal glutamate transporter EAAC1 by the interacting protein GTRAP3-18 [4].
  • Excitatory amino-acid carrier 1 (EAAC1) is a high-affinity Na+-dependent L-glutamate/D,L-aspartate cell-membrane transport protein [4].
  • The expression of GTRAP3-18 can be upregulated by retinoic acid, which results in a specific reduction of EAAC1-mediated glutamate transport [4].
  • However, the possible functional role of EAAC1 in preventing neuron death has not been resolved as compared with glial glutamate transporters such as GLT-1 [5].
  • Unique anti-apoptotic activity of EAAC1 in injured motor neurons [5].

Chemical compound and disease context of Slc1a1

  • The purpose of the current investigation was to determine whether the pharmacological properties of EAAC1 parallel those observed in cortical or cerebellar synaptosomes, C6 glioma, or primary astrocyte-enriched cultures [6].

Biological context of Slc1a1


Anatomical context of Slc1a1

  • It localizes to the cell membrane and cytoplasm, and specifically interacts with carboxy-terminal intracellular domain of EAAC1 [4].
  • This unique anti-apoptotic mechanism of EAAC1 functions in rescuing PC12 cells and motor neurons from NGF deprivation and nerve injury, respectively [5].
  • Immunoelectron microscopic study revealed that the perinuclear EAAC1 immunoreactivity corresponded to the translocation to the Golgi complex [11].
  • As an early alteration, perinuclear deposits of EAAC1 protein were found mainly in the large pyramidal neurons at the hippocampus, neocortex, piriform cortex, and amygdala with the reduction of neuropil staining 6 hours after KA injection [11].
  • In previous studies, we have shown that activation of protein kinase C (PKC) rapidly (within minutes) increases the activity and cell surface expression of the glutamate transporter EAAC1 in two systems that endogenously express this transporter (C6 glioma cells and cocultures of neurons and astrocytes) [12].

Associations of Slc1a1 with chemical compounds

  • In brain, EAAC1 is the primary neuronal glutamate transporter [4].
  • One candidate for this binding site, the highly conserved glutamic acid residue Glu-373 of EAAC1, was mutated to glutamine [7].
  • However, it was also speculated that the cotransported proton is shared in a H(+)-binding network, possibly involving the conserved histidine 295 in the sixth transmembrane domain of EAAC1 [13].
  • Together, these results suggest that histidine 295 is not protonated in EAAC1 at physiological pH and, thus, does not contribute to H(+) cotransport [13].
  • Our results show that replacement of H295 with glutamine, an amino acid residue that cannot be protonated, generates a fully functional transporter with transport kinetics that are close to those of the wild-type EAAC1 [13].
  • In hippocampal cultures, brief (5 min), robust (100 microM NMDA, 10 microM glycine) activation of the NMDA receptor decreased biotinylated EAAC1 to approximately 50% of control levels [14].

Other interactions of Slc1a1


Analytical, diagnostic and therapeutic context of Slc1a1


  1. A carboxyl-terminal determinant of the neuronal glutamate transporter, EAAC1, is required for platelet-derived growth factor-dependent trafficking. Sheldon, A.L., González, M.I., Robinson, M.B. J. Biol. Chem. (2006) [Pubmed]
  2. Evidence of neuronal excitatory amino acid carrier 1 expression in rat dorsal root ganglion neurons and their central terminals. Tao, F., Liaw, W.J., Zhang, B., Yaster, M., Rothstein, J.D., Johns, R.A., Tao, Y.X. Neuroscience (2004) [Pubmed]
  3. Plasma membrane and vesicular glutamate transporter mRNAs/proteins in hypothalamic neurons that regulate body weight. Collin, M., Bäckberg, M., Ovesjö, M.L., Fisone, G., Edwards, R.H., Fujiyama, F., Meister, B. Eur. J. Neurosci. (2003) [Pubmed]
  4. Modulation of the neuronal glutamate transporter EAAC1 by the interacting protein GTRAP3-18. Lin, C.I., Orlov, I., Ruggiero, A.M., Dykes-Hoberg, M., Lee, A., Jackson, M., Rothstein, J.D. Nature (2001) [Pubmed]
  5. Unique anti-apoptotic activity of EAAC1 in injured motor neurons. Kiryu-Seo, S., Gamo, K., Tachibana, T., Tanaka, K., Kiyama, H. EMBO J. (2006) [Pubmed]
  6. Comparison of Na+-dependent glutamate transport activity in synaptosomes, C6 glioma, and Xenopus oocytes expressing excitatory amino acid carrier 1 (EAAC1). Dowd, L.A., Coyle, A.J., Rothstein, J.D., Pritchett, D.B., Robinson, M.B. Mol. Pharmacol. (1996) [Pubmed]
  7. Is the glutamate residue Glu-373 the proton acceptor of the excitatory amino acid carrier 1? Grewer, C., Watzke, N., Rauen, T., Bicho, A. J. Biol. Chem. (2003) [Pubmed]
  8. Dexamethasone modulates the development of morphine tolerance and expression of glutamate transporters in rats. Wen, Z.H., Wu, G.J., Chang, Y.C., Wang, J.J., Wong, C.S. Neuroscience (2005) [Pubmed]
  9. Accumulation of [3H] glutamate in cultured rat calvarial osteoblasts. Takarada, T., Hinoi, E., Fujimori, S., Tsuchihashi, Y., Ueshima, T., Taniura, H., Yoneda, Y. Biochem. Pharmacol. (2004) [Pubmed]
  10. Effect of zonisamide on molecular regulation of glutamate and GABA transporter proteins during epileptogenesis in rats with hippocampal seizures. Ueda, Y., Doi, T., Tokumaru, J., Willmore, L.J. Brain Res. Mol. Brain Res. (2003) [Pubmed]
  11. Translocation of glutamate transporter subtype excitatory amino acid carrier 1 protein in kainic acid-induced rat epilepsy. Furuta, A., Noda, M., Suzuki, S.O., Goto, Y., Kanahori, Y., Rothstein, J.D., Iwaki, T. Am. J. Pathol. (2003) [Pubmed]
  12. Regulation of the neuronal glutamate transporter excitatory amino acid carrier-1 (EAAC1) by different protein kinase C subtypes. González, M.I., Kazanietz, M.G., Robinson, M.B. Mol. Pharmacol. (2002) [Pubmed]
  13. The conserved histidine 295 does not contribute to proton cotransport by the glutamate transporter EAAC1. Tao, Z., Grewer, C. Biochemistry (2005) [Pubmed]
  14. N-methyl-D-aspartate receptor-dependent regulation of the glutamate transporter excitatory amino acid carrier 1. Waxman, E.A., Baconguis, I., Lynch, D.R., Robinson, M.B. J. Biol. Chem. (2007) [Pubmed]
  15. Long-term alterations in glutamate receptor and transporter expression following early-life seizures are associated with increased seizure susceptibility. Zhang, G., Raol, Y.S., Hsu, F.C., Brooks-Kayal, A.R. J. Neurochem. (2004) [Pubmed]
  16. Differential effects of acute and chronic exercise on plasticity-related genes in the rat hippocampus revealed by microarray. Molteni, R., Ying, Z., Gómez-Pinilla, F. Eur. J. Neurosci. (2002) [Pubmed]
WikiGenes - Universities