Disease relevance of RP13-122B23.3

• NELF-B is identical to COBRA1, recently reported to associate with the product of breast cancer susceptibility gene BRCA1 [1].
• Using small interfering RNA technology with gastric cancer cells, we have shown that COBRA1 inhibition leads to increased TFF1 promoter activity and gene expression [2].
• In this work, we show that COBRA1 is overexpressed in the majority of primary upper gastrointestinal adenocarcinomas (UGC), and its overexpression correlates with down-regulation of TFF1 [2].
• The purpose of the study was to confirm that the Cobra catheter delivers adequate cerebral and systemic perfusion while providing simultaneous cerebral hypothermia and systemic normothermia during cardiopulmonary bypass [3].
• Two cDNAs encoding PLA2 isoenzymes from Taiwan Cobra (Naja naja atra) were cloned into pQE-30 plasmid vector and expressed in Escherichia coli [4].

High impact information on RP13-122B23.3

• Chromatin immunoprecipitation reveals that recruitment of COBRA1 and the other NELF subunits to endogenous ERalpha-responsive promoters is greatly stimulated upon estrogen treatment [5].
• Interestingly, COBRA1 does not affect the estrogen-dependent assembly of transcription regulatory complexes at the ERalpha-regulated promoters [5].
• A novel cofactor of BRCA1 (COBRA1) is recruited to the chromosome site by the first BRCT repeat of BRCA1, and is itself sufficient to induce chromatin unfolding [6].
• Our results suggest COBRA1 as a novel oncogene in UGCs that regulate AP-1 binding and the expression of TFF1 in upper gastric epithelia [2].
• Promoter analysis of TFF1 indicated that regulation of TFF1 by COBRA1 is estrogen independent in contrast to breast cancer [2].

Chemical compound and disease context of RP13-122B23.3

• The possible role of serum complement system in the formal pathogenesis of acute pancreatitis II. Cobra venom factor pancreatitis--sodiumtaurocholate and deoxycholate pancreatitis [7].

Biological context of RP13-122B23.3

• These findings suggest that COBRA1 may directly modulate AP-1 pathway and, therefore, may play important roles in cell proliferation, differentiation, apoptosis, and oncogenesis [8].
• Lack of c-Fos binding site in the COBRA1 completely abolished the COBRA1 inhibition of AP-1 trans-activation [8].
• The complete amino acid sequence of a cardiotoxin from the venom of Naja naja (Cambodian Cobra) [9].
• Effectiveness of the Cobra aortic catheter for dual-temperature management during adult cardiac surgery [3].
• Like other microtubule-interfering agents, COBRA-1 activated the proapoptotic c-Jun N-terminal kinase (JNK) signal transduction pathway, as evidenced by rapid induction of c-jun expression [10].

Anatomical context of RP13-122B23.3

• In this study, we demonstrate that COBRA1 mRNA was differentially expressed in breast cancer cell lines by semi-quantitative reverse transcription-polymerase chain PCR (RT-PCR) [11].
• Treatment of human breast cancer and brain tumor cells with COBRA-1 caused destruction of microtubule organization and apoptosis [12].
• RESULTS: Surgeons at 3 institutions placed the Cobra catheter in patients undergoing coronary artery bypass grafting (n = 13), valve (n = 3), and combined valve-bypass (n = 4) operations [3].
• Getting the tube in the oesophagus and oxygen in the trachea: preliminary results with the new supraglottic device (Cobra) in 28 anaesthetised patients [13].
• OBJECTIVES: In animals the Cardeon Cobra catheter (Cardeon Corp, Cupertino, Calif) allows independent control of aortic arch and descending aortic temperatures and profoundly reduces cerebral embolization during bypass [3].

Associations of RP13-122B23.3 with chemical compounds

• 1. Cobra cystatin strongly inhibits cysteine proteinases of the papain family, but not calpain [14].
• We describe a functional assay which is based on the interaction of factor B from various animal species with Cobra Venom Factor, the snake C3b-analog [15].
• Our aim was to evaluate the performance of the Cobra Perilaryngeal Airway in a cohort study and in a randomised, controlled, crossover comparison study with the Classic Laryngeal Mask Airway [16].
• The sensitivity and specificity of the four ELISAs were tested with purified antigens and EDTA-plasma or Cobra venom factor-activated EGTA-plasma samples as a source of C3a and C5a [17].
• Complete amino acid sequence of an acidic, cardiotoxic phospholipase A2 from the venom of Ophiophagus hannah (King Cobra): a novel cobra venom enzyme with "pancreatic loop" [18].

Physical interactions of RP13-122B23.3

• COBRA1 physically interacted with the AP-1 family members, c-Jun and c-Fos, and the middle region of COBRA1 bound to c-Fos [8].
• Endogenously expressed COBRA1 interacted with the nuclear protein BRCA1 in human breast cancer cells [11].

Regulatory relationships of RP13-122B23.3

• COBRA-1 inhibited GTP-induced tubulin polymerization in cell-free tubulin turbidity assays [10].

Other interactions of RP13-122B23.3

• Electrophoretic mobility shift assay showed that COBRA1 attenuates AP-1 binding to DNA [2].
• Simultaneous loss of TFF1 expression and overexpression of COBRA1 was observed in 42 of 70 (60%) tumors [2].
• Cofactor of BRCA1 (COBRA1) is a newly characterized member of the negative elongation factor (NELF) complex [2].
• Cofactor of BRCA1 (COBRA1), a novel protein, was isolated as a BRCA1-interacting protein [11].
• METHODS: One hundred thirty-two patients in 20 centers underwent radiofrequency ablation as an adjunct to conventional cardiac surgery, with a standardized lesion set created with a flexible, 7-electrode, temperature-controlled probe (Cobra; EPTechnologies, San Jose, Calif) [19].

Analytical, diagnostic and therapeutic context of RP13-122B23.3

• Immunofluorescence analysis indicated that COBRA1 was a nuclear protein [11].
• In most cases, the levels of COBRA1 protein in breast cancer cell lines detected by Western blotting with the anti-COBRA1 antibody correlated with those of COBRA1 mRNA [11].
• In seven patients, including two who had unsuccessful wire/balloon angioplasty, an over the wire system was used through a 6.5F Cobra or 7F JR4 guide catheter, selectively inserted into the gastroduodenal artery [20].
• Comparison of three disposable extraglottic airway devices in spontaneously breathing adults: the LMA-Unique, the Soft Seal laryngeal mask, and the Cobra perilaryngeal airway [21].
• CONCLUSIONS: The Cobra device met all expectations for an arterial cannula with adequate perfusion to the arch and corporeal circulations [3].

References