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Nmt1  -  N-myristoyltransferase 1

Rattus norvegicus

Synonyms: Glycylpeptide N-tetradecanoyltransferase 1, Myristoyl-CoA:protein N-myristoyltransferase 1, NMT 1, Peptide N-myristoyltransferase 1, Type I N-myristoyltransferase
 
 
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Disease relevance of Nmt1

  • This is the first study demonstrating the expression and alteration of NMT localization in cardiac ischemia and pertaining to a possible role of co-translational modification of proteins in cardiac functions and injury [1].
  • Synthetic peptides of known myristoylated proteins--pp60src and cyclic adenosine monophosphate-dependent protein kinase--were used in kinetic analyses of N-myristoyltransferase in colonic cancer and normal-appearing colonic tissue [2].
  • CONCLUSIONS: This study demonstrates for the first time that N-myristoyltransferase activity is higher in colonic epithelial neoplasms than in normal-appearing colonic tissue and that an increase in N-myristoyltransferase activity appears at an early stage in colonic carcinogenesis [2].
  • Elevation of N-myristoyltransferase activity was present in all tumors, including colonic polyps [2].
  • Studies of the catalytic activities and substrate specificities of Saccharomyces cerevisiae myristoyl-coenzyme A: protein N-myristoyltransferase deletion mutants and human/yeast Nmt chimeras in Escherichia coli and S. cerevisiae [3].
 

High impact information on Nmt1

  • RESULTS: N-Myristoyltransferase activity was increased in rat colonic tumors compared with normal-appearing adjacent mucosa and normal mucosa (P = .0002) [2].
  • METHODS: N-Myristoyltransferase activity was determined in 45 colonic tissue specimens from Sprague-Dawley rats--10 given injections of the colon carcinogen, azoxymethane, and three untreated [2].
  • N-Myristoyltransferase of colonic cancer tissues had a similar Michaelis constant but an approximate twofold higher maximum velocity for both the pp60src- and cyclic adenosine monophosphate-dependent protein kinase-derived peptides compared with N-myristoyltransferase of normal-appearing tissue [2].
  • Octapeptides derived from the NH2-terminal sequences of these four G alpha polypeptides were tested as substrates for purified S. cerevisiae NMT [4].
  • A dual plasmid system was used to examine the protein and acyl-CoA specificities of Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase (NMT) by co-expressing it in Escherichia coli with each of four homologous alpha subunits of the signal-transducing, heterotrimeric G proteins [4].
 

Chemical compound and disease context of Nmt1

 

Biological context of Nmt1

 

Anatomical context of Nmt1

 

Associations of Nmt1 with chemical compounds

 

Other interactions of Nmt1

 

Analytical, diagnostic and therapeutic context of Nmt1

  • In the present study, we investigated the expression and activity of NMT in rat heart after ischemia and reperfusion [1].
  • Western blot analysis of rat heart samples indicated a prominent immunoreactive band of 66 kDa probed with human NMT antibody [1].
  • Colectomy specimens from five patients were assayed for N-myristoyltransferase activity [2].
  • STZ-induced diabetes (an animal model for insulin-dependent diabetes mellitus, IDDM) resulted in a 2-fold increase in rat liver NMT activity as compared with control animals [9].
  • Examination of the polyspecificity profile of NMT-1 mAbs was also undertaken using a panel of 23 antigens including heterologous proteins, phospholipids, haptens and bacterial antigens by antibody titration and competition ELISAs [15].

References

  1. Altered expression and localization of N-myristoyltransferase in experimentally induced rat model of ischemia-reperfusion. Rajala, R.V., Kakkar, R., Kanthan, R., Radhi, J.M., Wang, X., Wang, R., Datla, R.S., Sharma, R.K. J. Cell. Biochem. (2002) [Pubmed]
  2. Increased N-myristoyltransferase activity observed in rat and human colonic tumors. Magnuson, B.A., Raju, R.V., Moyana, T.N., Sharma, R.K. J. Natl. Cancer Inst. (1995) [Pubmed]
  3. Studies of the catalytic activities and substrate specificities of Saccharomyces cerevisiae myristoyl-coenzyme A: protein N-myristoyltransferase deletion mutants and human/yeast Nmt chimeras in Escherichia coli and S. cerevisiae. Rudnick, D.A., Johnson, R.L., Gordon, J.I. J. Biol. Chem. (1992) [Pubmed]
  4. Analyzing the substrate specificity of Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase by co-expressing it with mammalian G protein alpha subunits in Escherichia coli. Duronio, R.J., Rudnick, D.A., Adams, S.P., Towler, D.A., Gordon, J.I. J. Biol. Chem. (1991) [Pubmed]
  5. Membrane-associated N-myristoyltransferase activity is reduced in obese (fa/fa) Zucker rat liver. King, M.J., Pugazhenthi, S., Khandelwal, R.L., Sharma, R.K. Biochem. Biophys. Res. Commun. (1993) [Pubmed]
  6. Design and synthesis of novel benzofurans as a new class of antifungal agents targeting fungal N-myristoyltransferase. Part 2. Ebiike, H., Masubuchi, M., Liu, P., Kawasaki, K., Morikami, K., Sogabe, S., Hayase, M., Fujii, T., Sakata, K., Shindoh, H., Shiratori, Y., Aoki, Y., Ohtsuka, T., Shimma, N. Bioorg. Med. Chem. Lett. (2002) [Pubmed]
  7. A radioresistant variant cell line, NMT-1R, isolated from a radiosensitive rat yolk sac tumour cell line, NMT-1: differences of early radiation-induced morphological changes, especially apoptosis. Mitsuhashi, N., Takahashi, T., Sakurai, H., Nozaki, M., Akimoto, T., Hasegawa, M., Saito, Y., Matsumoto, H., Higuchi, K., Maebayashi, K., Niibe, H. Int. J. Radiat. Biol. (1996) [Pubmed]
  8. Cytotoxic effect of paclitaxel (taxol) either alone or in combination with irradiation in two rat yolk sac tumour cell lines with different radiosensitivities in vitro. Saito, Y., Mitsuhashi, N., Takahashi, T., Sakurai, H., Nozaki, M., Ishikawa, H., Nasu, S., Hayakawa, K., Niibe, H. Int. J. Radiat. Biol. (1998) [Pubmed]
  9. In vivo modulation of N-myristoyltransferase activity by orthovanadate. King, M.J., Pugazhenthi, S., Khandelwal, R.L., Sharma, R.K. Mol. Cell. Biochem. (1995) [Pubmed]
  10. N-myristoylation of p60src. Identification of a myristoyl-CoA:glycylpeptide N-myristoyltransferase in rat tissues. Glover, C.J., Goddard, C., Felsted, R.L. Biochem. J. (1988) [Pubmed]
  11. Establishment and characterization of a rat yolk sac tumor cell line, NMT-1, producing alpha-fetoprotein, with potential for lymphatic metastasis. Mitsuhashi, N., Takahashi, T., Nozaki, M., Matsumoto, H., Sakurai, H., Takahashi, M., Niibe, H. Jpn. J. Cancer Res. (1993) [Pubmed]
  12. Increased myocardial N-myristoyltransferase activity in rotenone model of Parkinsonism. Pasha, M.K., Sharma, R.K., Rajput, A.H. Int. J. Mol. Med. (2005) [Pubmed]
  13. A simplified assay for the enzyme responsible for the attachment of myristic acid to the N-terminal glycine residue of proteins, myristoyl-CoA: glycylpeptide N-myristoyltransferase. McIlhinney, R.A., McGlone, K. Biochem. J. (1989) [Pubmed]
  14. Hyperthermia-induced apoptosis in two rat yolk sac tumor cell lines with different radiothermosensitivity in vitro. Islam, M.S., Mitsuhashi, N., Akimoto, T., Sakurai, H., Hasegawa, M., Ishikawa, H., Niibe, H. Oncol. Rep. (2001) [Pubmed]
  15. Naturally-occurring anti-thymocyte autoantibody which identifies a restricted CD4+CD8+CD3-/lo/int thymocyte subpopulation exhibits extensive polyspecificity. Underwood, J.R., McCall, A., Csar, X.F. Thymus (1996) [Pubmed]
 
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