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TSKU  -  tsukushi, small leucine rich proteoglycan

Homo sapiens

Synonyms: E2-induced gene 4 protein, E2IG4, LRRC54, Leucine-rich repeat-containing protein 54, TSK, ...
 
 
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Disease relevance of TSKU

 

Psychiatry related information on TSKU

  • Motor function (ROM; kinaesthetic sense; activity of the superficial neck flexors (EMG) during cranio-cervical flexion), quantitative sensory testing (pressure, thermal pain thresholds and brachial plexus provocation test), sympathetic vasoconstrictor responses and psychological distress (GHQ-28, TSK and IES) were measured [6].
  • The association between these physical activity related variables and perceived disability (QBPDS), fear of movement/(re)injury (TSK), pain catastrophizing (PCS) and pain intensity (VAS) was examined [7].
  • Thirty-seven patients with medically intractable unilateral temporal lobe epilepsy (16-55 years of age; 20 right temporal and 17 left temporal foci) were examined with standardized personality inventories (FPI, STAI, IPC, TSK) supplemented by a rating scale evaluated by the neuropsychologist (GEWLE) [8].
 

High impact information on TSKU

 

Biological context of TSKU

  • Incubation of fetal sheep serum with [125I]oIGF-II, followed by chromatography on Fractogel TSK HW55(S), revealed the presence of two major binding protein species, a lower molecular weight binding protein (apparent mol wt approximately 60,000) and a much higher molecular weight binding protein (apparent mol wt approximately 500,000) [14].
  • Chromatographic separation and determination of tryptophan in food-stuffs after barytic hydrolysis using Fractogel TSK HW 40 S [15].
  • We have generated two distinct genotypes of mice, TSK/+ and +/+ with respect to the TSK trait by breeding TSK mice with immunodeficient mouse strains [16].
  • To investigate the specific relationship between HNElastase inhibition and specificity of molecular structure of several NSAIDs, HNElastase was purified by Ultrogel AcA54 gel filtration, CM-Sephadex ion exchange, and HPLC (with TSK 250 column) chromatography [17].
  • Sera from a patient with macroprolactinoma (case 1) and from a hyperprolactinemic woman with regular menstruation (case 2) were analyzed for prolactin activity by gel filtration using Sephadex G-100, Sephadex G-200 and TSK G3000SW columns [18].
 

Anatomical context of TSKU

  • A substrate protein for botulinum C3 ADP-ribosyltransferase (C3 exoenzyme) in human platelets was purified to apparent homogeneity from the cytosol by ammonium sulfate fractionation and successive chromatography on columns of DEAE-Sepharose, hydroxylapatite, phenyl-Sepharose, and TSK phenyl-5PW [19].
  • A polypeptide growth factor, Harderian gland-derived growth factor (HGDGF), has been purified approximately 43,000-fold from guinea pig Harderian gland by column chromatography on TSK gel DEAE-5PW, blue-Sepharose CL-6B, and Superose 12 [20].
  • High performance liquid chromatography with TSK 5000 PW or TSK 6000 PW size exclusion columns combined with a 125I labelled hyaluronic acid binding protein assay was used to study the effects of oxygen derived free radicals on synovial fluid hyaluronate [21].
  • Inhibition of fibrosis in TSK mice by blocking mast cell degranulation [22].
  • The HPLC characteristics of extensively purified chicken oviduct progesterone receptors were compared on TSK 3000 SW size-exclusion and DEAE-5-PW media packed in either glass or stainless-steel columns [23].
 

Associations of TSKU with chemical compounds

 

Analytical, diagnostic and therapeutic context of TSKU

  • Acid protease was partially purified about 3000-fold by Mono Q ion-exchange chromatography, pepstatin-aminohexyl-Sepharose affinity chromatography, hydroxylapatite affinity chromatography, lectin affinity chromatographies, and gel filtration on TSK G3000SW [29].
  • The molecular mass of PAF-releasing factor revealed on a TSK gel G3000 SW HPLC column was 240 kDa, which was different from that of albumin [30].
  • Binding of 125I-rat SC to Ig was determined in two ways: 1) immunoprecipitation of putative 125I-rat SC-Ig complexes with anti-L chain antibodies; 2) HPLC gel filtration on an analytical TSK 4000 column that separated free 125I-rat SC from 125I-rat SC bound to Ig [31].
  • Purification involved: volume reduction, ultracentrifugation to remove pectin and co-adsorbed cationic peroxidase, followed by chromatography of anionic extensin peroxidase (pl 4.6) on DEAE-Trisacryl and TSK-gel DEAE-5PW columns [32].
  • The enzyme partially purified by sequential chromatography on GTP-agarose, DEAE-cellulose, and TSK 3000 (0.6 X 60 mm) columns eluted as a 300-450 kDa protein which appeared as 60-90 kDa polypeptides on SDS-PAGE [33].

References

  1. Increased expression of type I collagen induced by microfibril-associated glycoprotein 2: novel mechanistic insights into the molecular basis of dermal fibrosis in scleroderma. Lemaire, R., Korn, J.H., Shipley, J.M., Lafyatis, R. Arthritis Rheum. (2005) [Pubmed]
  2. The major ribonucleoprotein-associated protein kinase of vesicular stomatitis virus is a host cell protein. Harmon, S.A., Marnell, L.L., Summers, D.F. J. Biol. Chem. (1983) [Pubmed]
  3. Isolation of apolipoprotein-B-48 from chylomicrons by high-performance liquid chromatography. Wagner, H.A., Nustede, R., Seidel, D. J. Chromatogr. (1987) [Pubmed]
  4. A liquid chromatographic preparation of retroviral RNA. Pager, J. Anal. Biochem. (1993) [Pubmed]
  5. High pressure liquid chromatographic detection of intracellular retinoid binding proteins from cultured cell and tumor cytosols. Allegretto, E.A., Kelly, M.A., Donaldson, C.A., Levine, N., Pike, J.W., Haussler, M.R. Biochem. Biophys. Res. Commun. (1983) [Pubmed]
  6. Physical and psychological factors maintain long-term predictive capacity post-whiplash injury. Sterling, M., Jull, G., Kenardy, J. Pain (2006) [Pubmed]
  7. Decline in physical activity, disability and pain-related fear in sub-acute low back pain. Verbunt, J.A., Sieben, J.M., Seelen, H.A., Vlaeyen, J.W., Bousema, E.J., van der Heijden, G.J., Knottnerus, J.A. European journal of pain (London, England) (2005) [Pubmed]
  8. On the psychopathology of unilateral temporal lobe epilepsy. Feddersen, B., Herzer, R., Hartmann, U., Gaab, M.R., Runge, U. Epilepsy & behavior : E&B. (2005) [Pubmed]
  9. Purification of a 110-kilodalton cytosolic phospholipase A2 from the human monocytic cell line U937. Clark, J.D., Milona, N., Knopf, J.L. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  10. Effects of estrogen on global gene expression: identification of novel targets of estrogen action. Charpentier, A.H., Bednarek, A.K., Daniel, R.L., Hawkins, K.A., Laflin, K.J., Gaddis, S., MacLeod, M.C., Aldaz, C.M. Cancer Res. (2000) [Pubmed]
  11. The effect of calmodulin inhibitors with bleomycin on the treatment of patients with high grade gliomas. Hait, W.N., Byrne, T.N., Piepmeier, J., Durivage, H.J., Choudhury, S., Davis, C.A., Gates, J.A. Cancer Res. (1990) [Pubmed]
  12. Constitutive production of interleukin 1 by human monocytic leukemia cell line JOSK-I and the production mechanism. Furukawa, Y., Ohta, M., Kasahara, T., Miura, Y., Saito, M. Cancer Res. (1987) [Pubmed]
  13. Steroid and xenobiotic receptor SXR mediates vitamin K2-activated transcription of extracellular matrix-related genes and collagen accumulation in osteoblastic cells. Ichikawa, T., Horie-Inoue, K., Ikeda, K., Blumberg, B., Inoue, S. J. Biol. Chem. (2006) [Pubmed]
  14. Fetal sheep serum contains a high molecular weight insulin-like growth factor (IGF) binding protein that is acid stable and specific for IGF-II. Hey, A.W., Browne, C.A., Thorburn, G.D. Endocrinology (1987) [Pubmed]
  15. Chromatographic separation and determination of tryptophan in food-stuffs after barytic hydrolysis using Fractogel TSK HW 40 S. Huet, J.C., Pernollet, J.C. J. Chromatogr. (1986) [Pubmed]
  16. Correlation between the concentration of serum anti-topoisomerase I autoantibodies and histological and biochemical alterations in the skin of tight skin mice. Hatakeyama, A., Kasturi, K.N., Wolf, I., Phelps, R.G., Bona, C.A. Cell. Immunol. (1996) [Pubmed]
  17. Molecular characteristics of the inhibition of human neutrophil elastase by nonsteroidal antiinflammatory drugs. Kang, K., Bae, S.J., Kim, W.M., Lee, D.H., Cho, U., Lee, M.H., Lee, M.S., Nam, S., Kuettner, K.E., Schwartz, D.E. Exp. Mol. Med. (2000) [Pubmed]
  18. Heterogeneity of big-big hPRL in hyperprolactinemia. Tanaka, T., Yano, H., Umezawa, S., Shishiba, Y., Okada, K., Saito, T., Hibi, I. Horm. Metab. Res. (1989) [Pubmed]
  19. A rho gene product in human blood platelets. I. Identification of the platelet substrate for botulinum C3 ADP-ribosyltransferase as rhoA protein. Nemoto, Y., Namba, T., Teru-uchi, T., Ushikubi, F., Morii, N., Narumiya, S. J. Biol. Chem. (1992) [Pubmed]
  20. Purification and characterization of a growth factor from guinea pig harderian gland. Yokoyama, Y., Kano, K., Kaji, K., Seyama, Y. J. Biol. Chem. (1989) [Pubmed]
  21. Oxygen derived free radicals and synovial fluid hyaluronate. Saari, H. Ann. Rheum. Dis. (1991) [Pubmed]
  22. Inhibition of fibrosis in TSK mice by blocking mast cell degranulation. Walker, M.A., Harley, R.A., LeRoy, E.C. J. Rheumatol. (1987) [Pubmed]
  23. High-yield high-performance liquid chromatographic analysis of steroid hormone receptors on glass columns. Denner, L.A., Weigel, N.L., Schrader, W.T., O'Malley, B.W. Anal. Biochem. (1987) [Pubmed]
  24. Amino acid-specific ADP-ribosylation. Evidence for two distinct NAD:arginine ADP-ribosyltransferases in turkey erythrocytes. Yost, D.A., Moss, J. J. Biol. Chem. (1983) [Pubmed]
  25. Peptide separation in normal phase liquid chromatography. Yoshida, T. Anal. Chem. (1997) [Pubmed]
  26. A new on-line dual enzymatic method for simultaneous quantification of cholesterol and triglycerides in lipoproteins by HPLC. Usui, S., Hara, Y., Hosaki, S., Okazaki, M. J. Lipid Res. (2002) [Pubmed]
  27. Liquid-chromatographic assay for free and transthyretin-bound retinol-binding protein in serum from normal humans. Burri, B.J., Kutnink, M.A. Clin. Chem. (1989) [Pubmed]
  28. High resolution and high sensitivity methods for oligosaccharide mapping and characterization by normal phase high performance liquid chromatography following derivatization with highly fluorescent anthranilic acid. Anumula, K.R., Dhume, S.T. Glycobiology (1998) [Pubmed]
  29. Acid protease secreted from human pancreatic carcinoma cell line HPC-YT into serum-free, chemically defined medium. Yamaguchi, N., Kawai, K. Cancer Res. (1986) [Pubmed]
  30. Release of newly synthesized platelet-activating factor (PAF) from human polymorphonuclear leukocytes under in vivo conditions. Contribution of PAF-releasing factor in serum. Miwa, M., Sugatani, J., Ikemura, T., Okamoto, Y., Ino, M., Saito, K., Suzuki, Y., Matsumoto, M. J. Immunol. (1992) [Pubmed]
  31. Rat secretory component binds poorly to rodent IgM. Underdown, B.J., Switzer, I., Jackson, G.D. J. Immunol. (1992) [Pubmed]
  32. Isolation of pl 4.6 extensin peroxidase from tomato cell suspension cultures and identification of Val-Tyr-Lys as putative intermolecular cross-link site. Schnabelrauch, L.S., Kieliszewski, M., Upham, B.L., Alizedeh, H., Lamport, D.T. Plant J. (1996) [Pubmed]
  33. Specific cleavage of recombinant protein tau3 between valine-220 and tyrosine-221 (val-309 and tyr-310 of tau4) by a double-stranded DNA-stimulated protease. Wu, J.M., An, S., Ng, C., Scott, C.W., Caputo, C.B. Biochem. Biophys. Res. Commun. (1996) [Pubmed]
 
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