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Gene Review

LanA  -  Laminin A

Drosophila melanogaster

Synonyms: 5]], CG10236, CT28769, Dmel\CG10236, FBgn0002526, ...
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Disease relevance of LanA


High impact information on LanA


Biological context of LanA

  • A novel method of tandemly fused RNA probes showed that developmental increases of laminin mRNAs were primarily associated with periods of morphogenesis, and preceded those of collagen IV, a protein strongly expressed during growth [7].
  • Homologs of the G-subdomains of this A chain, which occur in interacting regions of agrin, perlecan, laminin and sex steroid binding protein, may be involved in protein associations [7].
  • A Drosophila laminin A chain gene was characterized as a 14 kb genomic nucleotide sequence which encodes an open reading frame of 3712 amino acids in 15 exons [7].
  • These phenotypes suggest that laminin A has diverse functions during morphogenesis in Drosophila [8].
  • Recent studies ascribed some biological actions of cell adhesion and cell outgrowth to the carboxyl-most 1200 amino acids of vertebrate laminin A chains [9].

Anatomical context of LanA


Associations of LanA with chemical compounds

  • Phenotypic similarities suggest that laminin A is a potential ligand for this integrin, at least in some tissues [14].
  • Furthermore, laminin-dependent BG2-c6 cell spreading was inhibited by heparin [12].
  • Its murine counterpart carries the sequence SIKVAV by which the interaction of laminin and cells is mediated [12].
  • Two Ciona alpha chains cluster in laminin-binding clade and the remaining chain clusters in the clade that binds the RGD tripeptide sequence [15].
  • This proteoglycan-like glycoprotein differs substantially from corresponding proteoglycans found in vertebrate basement membranes, in contrast to Drosophila basement membrane laminin and collagen IV which have been conserved evolutionarily [16].

Regulatory relationships of LanA

  • These data indicate that the Drosophila integrin cascade triggered by the interaction between BG2-c6 and laminin was initiated at the integrin cluster with tyrosine-phosphorylated proteins, similar to the observations in vertebrate cells [17].

Other interactions of LanA

  • The role of cell adhesion molecules in Drosophila heart morphogenesis: faint sausage, shotgun/DE-cadherin, and laminin A are required for discrete stages in heart development [18].
  • Drosophila doubly heterozygous for slit and the genes encoding the integrin ligands Laminin A and Tiggrin reveal similar errors in midline axon guidance [19].
  • Furthermore, Dg depends on Pcan but not on Laminin A for its localization in the basal-cell membrane, and the two proteins bind in vitro [20].
  • The expression of several additional interactors--viking (vkg), Cg25, and laminin-alpha (LanA)-is affected only in a nonautonomous manner [21].
  • Absence of PS integrins or laminin A affects extracellular adhesion, but not intracellular assembly, of hemiadherens and neuromuscular junctions in Drosophila embryos [22].

Analytical, diagnostic and therapeutic context of LanA

  • Developmental analyses were undertaken using light and electron microscopy of wild-type and bs wings as well as confocal microscopy of phalloidin- and laminin-stained preparations. bs defects were first seen early in the prepupal period with the failure of apposition of dorsal and ventral wing epithelia [23].
  • Previously, an overlapping set of dodecapeptides that covered a region of the murine laminin alpha1 chain similar to recalphaS had been synthesized and tested for cell culture support properties (Nomizu, M., Kim, W. H., Yamamura, K., Utani, A., Otaka, A., Roller, P. P., Kleinman, H. K., and Yamada, Y. (1995) J. Biol. Chem. 270, 20583-20590) [24].
  • Sequence analysis of the cDNA for the alpha 3B predicts a polypeptide of 2541 amino acids (279,510 Da) comprising a truncated short arm and a carboxyl-terminal long arm common to the laminin alpha chains identified thus far [13].
  • Assembly of Drosophila laminin alpha, beta and gamma chains was analyzed by immunoprecipitation of the lysate from metabolically radiolabeled Kc 167 cells with chain-specific antibodies followed by two dimensional electrophoresis in which non-reducing and reducing SDS gel electrophoresis are combined [25].


  1. Substitution of a conserved cysteine-996 in a cysteine-rich motif of the laminin alpha2-chain in congenital muscular dystrophy with partial deficiency of the protein. Nissinen, M., Helbling-Leclerc, A., Zhang, X., Evangelista, T., Topaloglu, H., Cruaud, C., Weissenbach, J., Fardeau, M., Tomé, F.M., Schwartz, K., Tryggvason, K., Guicheney, P. Am. J. Hum. Genet. (1996) [Pubmed]
  2. Essential role of alpha 6 integrins in cortical and retinal lamination. Georges-Labouesse, E., Mark, M., Messaddeq, N., Gansmüller, A. Curr. Biol. (1998) [Pubmed]
  3. Interaction of Bombyx mori nucleopolyhedrovirus BRO-A and host cell protein laminin. Kang, W.K., Imai, N., Suzuki, M., Iwanaga, M., Matsumoto, S., Zemskov, E.A. Arch. Virol. (2003) [Pubmed]
  4. Expression of crumbs confers apical character on plasma membrane domains of ectodermal epithelia of Drosophila. Wodarz, A., Hinz, U., Engelbert, M., Knust, E. Cell (1995) [Pubmed]
  5. wing blister, a new Drosophila laminin alpha chain required for cell adhesion and migration during embryonic and imaginal development. Martin, D., Zusman, S., Li, X., Williams, E.L., Khare, N., DaRocha, S., Chiquet-Ehrismann, R., Baumgartner, S. J. Cell Biol. (1999) [Pubmed]
  6. Laminin in the male germ cells of Drosophila. Wang, F., Hanske, M., Miedema, K., Klein, G., Ekblom, P., Hennig, W. J. Cell Biol. (1992) [Pubmed]
  7. Laminin A chain: expression during Drosophila development and genomic sequence. Kusche-Gullberg, M., Garrison, K., MacKrell, A.J., Fessler, L.I., Fessler, J.H. EMBO J. (1992) [Pubmed]
  8. Genetic analysis of laminin A reveals diverse functions during morphogenesis in Drosophila. Henchcliffe, C., García-Alonso, L., Tang, J., Goodman, C.S. Development (1993) [Pubmed]
  9. Drosophila laminin A chain sequence, interspecies comparison, and domain structure of a major carboxyl portion. Garrison, K., MacKrell, A.J., Fessler, J.H. J. Biol. Chem. (1991) [Pubmed]
  10. Dystroglycan down-regulation links EGF receptor signaling and anterior-posterior polarity formation in the Drosophila oocyte. Poulton, J.S., Deng, W.M. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  11. Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila. Deng, W.M., Schneider, M., Frock, R., Castillejo-Lopez, C., Gaman, E.A., Baumgartner, S., Ruohola-Baker, H. Development (2003) [Pubmed]
  12. Active sites in the carboxyl-terminal region of the laminin alpha chain in Drosophila neuronal cell spreading. Takagi, Y., Nomizu, M., Ui-Tei, K., Tokushige, N., Hirohashi, S. Arch. Insect Biochem. Physiol. (2004) [Pubmed]
  13. Cloning and complete primary structure of the mouse laminin alpha 3 chain. Distinct expression pattern of the laminin alpha 3A and alpha 3B chain isoforms. Galliano, M.F., Aberdam, D., Aguzzi, A., Ortonne, J.P., Meneguzzi, G. J. Biol. Chem. (1995) [Pubmed]
  14. A novel alpha integrin subunit associates with betaPS and functions in tissue morphogenesis and movement during Drosophila development. Stark, K.A., Yee, G.H., Roote, C.E., Williams, E.L., Zusman, S., Hynes, R.O. Development (1997) [Pubmed]
  15. The integrins of the urochordate Ciona intestinalis provide novel insights into the molecular evolution of the vertebrate integrin family. Ewan, R., Huxley-Jones, J., Mould, A.P., Humphries, M.J., Robertson, D.L., Boot-Handford, R.P. BMC Evol. Biol. (2005) [Pubmed]
  16. Papilin: a Drosophila proteoglycan-like sulfated glycoprotein from basement membranes. Campbell, A.G., Fessler, L.I., Salo, T., Fessler, J.H. J. Biol. Chem. (1987) [Pubmed]
  17. Laminin-dependent integrin clustering with tyrosine-phosphorylated molecules in a Drosophila neuronal cell line. Takagi, Y., Ui-Tei, K., Miyake, T., Hirohashi, S. Neurosci. Lett. (1998) [Pubmed]
  18. The role of cell adhesion molecules in Drosophila heart morphogenesis: faint sausage, shotgun/DE-cadherin, and laminin A are required for discrete stages in heart development. Haag, T.A., Haag, N.P., Lekven, A.C., Hartenstein, V. Dev. Biol. (1999) [Pubmed]
  19. Integrins regulate responsiveness to slit repellent signals. Stevens, A., Jacobs, J.R. J. Neurosci. (2002) [Pubmed]
  20. Perlecan and Dystroglycan act at the basal side of the Drosophila follicular epithelium to maintain epithelial organization. Schneider, M., Khalil, A.A., Poulton, J., Castillejo-Lopez, C., Egger-Adam, D., Wodarz, A., Deng, W.M., Baumgartner, S. Development (2006) [Pubmed]
  21. Dose-sensitive autosomal modifiers identify candidate genes for tissue autonomous and tissue nonautonomous regulation by the Drosophila nuclear zinc-finger protein, hindsight. Wilk, R., Pickup, A.T., Hamilton, J.K., Reed, B.H., Lipshitz, H.D. Genetics (2004) [Pubmed]
  22. Absence of PS integrins or laminin A affects extracellular adhesion, but not intracellular assembly, of hemiadherens and neuromuscular junctions in Drosophila embryos. Prokop, A., Martín-Bermudo, M.D., Bate, M., Brown, N.H. Dev. Biol. (1998) [Pubmed]
  23. Blistered: a gene required for vein/intervein formation in wings of Drosophila. Fristrom, D., Gotwals, P., Eaton, S., Kornberg, T.B., Sturtevant, M., Bier, E., Fristrom, J.W. Development (1994) [Pubmed]
  24. Conserved neuron promoting activity in Drosophila and vertebrate laminin alpha1. Takagi, Y., Nomizu, M., Gullberg, D., MacKrell, A.J., Keene, D.R., Yamada, Y., Fessler, J.H. J. Biol. Chem. (1996) [Pubmed]
  25. Disulfide-bonding between Drosophila laminin beta and gamma chains is essential for alpha chain to form alpha betagamma trimer. Kumagai, C., Kadowaki, T., Kitagawa, Y. FEBS Lett. (1997) [Pubmed]
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