Disease relevance of DTL

• Taken together, our data suggest that Ramp plays a role in the proliferation of the human embryonal carcinoma cells [1].
• METHODS: Photopic flash ERGs were recorded differentially, with DTL electrodes, between the two eyes of 22 patients with diagnosed optic neuropathy (n = 17, anterior ischemic optic neuropathy [AION]; n = 5, compressive optic neuropathy) and 25 age-matched control subjects and in 17 eyes of 13 monkeys (Macaca mulatta) [2].
• The doses of DOC ranged from 40 mg/m2 up to the dose-limiting toxicity (DTL) [3].

High impact information on DTL

• Our data indicate that the rereplication reflects a requirement for DTL in regulating CDT1, a protein required for prereplication complex formation [4].
• Thus, DTL promotes genomic stability through two distinct mechanisms [4].
• We show that DTL associates with the CUL4-DDB1 E3 ubiquitin ligase and is required for CDT1 down-regulation in unperturbed cells and following DNA damage [4].
• Here, we show that CUL4-DDB1 complexes interact with multiple WD40-repeat proteins (WDRs) including TLE1-3, WDR5, L2DTL (also known as CDT2) and the Polycomb-group protein EED (also known as ESC) [5].
• In addition to nucleotide sequence, bioinformatic analysis has revealed a conservation of critical functional motifs among the human L2DTL, mouse L2dtl, and Drosophila l(2)dtl proteins [6].

Biological context of DTL

• 2. Associated with the nuclear matrix of NT2 cells, Ramp translocates from the interphase nucleus to the metaphase cytoplasm during mitosis [1].
• During the late stage of cytokinesis, Ramp concentrates at the midzone of the dividing daughter cells [1].
• CONCLUSIONS: Use of DTL electrodes to elicit electrical phosphenes is safe, fast, and reliable [7].
• Phosphenes Electrically Evoked with DTL Electrodes: A Study in Patients with Retinitis Pigmentosa, Glaucoma, and Homonymous Visual Field Loss and Normal Subjects [7].
• In contrast, DTL inhibits the proliferative activity of HeLa cells grown on solid substrate and acts as inductor of differentiation, slowing cell growth, increasing the cell attachment and spreading [8].

Anatomical context of DTL

• Although the amplitude of the signal collected by the DTL electrode varies from session to session, this is not a problem, as the photoreceptor fractional circulating current is obtained by normalization of the response family [9].
• A component with a latency that varied with distance from the optic nerve head was easily identified by eye in recordings from the speculum of a Burian-Allen electrode referenced to a DTL on the unstimulated eye [10].
• DTL was shown to increase the proliferation of HeLa cells grown in soft agar greatly (in anchorage-independent fashion) [8].
• Heaviest labelling occurred in the thin descending limb (DTL) of the loop of Henle in the inner stripe of the outer medulla, with apparent decreasing expression in the inner medulla, outer stripe of the outer medulla and cortex, but no quantitation was performed [11].
• We used RT-PCR, immunostaining, Western blotting, and centrosome isolation to determine the L2DTL expression and protein localization, and RNAi to analyze its role in tumor cell growth [12].

Associations of DTL with chemical compounds

• Using RNA fingerprinting by arbitrarily primed polymerase chain reaction, we identified a novel serine/threonine-rich protein, RA-regulated nuclear matrix-associated protein (Ramp), that was down-regulated during the RA-induced differentiation of NT2 cells [1].
• We found herein that N-acetyl-D-glucosamine-specific lectin, isolated from the ascidian Didemnum ternatanum (DTL), alters the growth properties of HeLa tumor cells depending on the anchorage [8].
• Previously we isolated GlcNAc-specific lectin (DTL) from the ascidian Didemnum ternatanum by affinity chromatography on cross-linked ovalbumin [13].
• Hapten inhibition with various sugars indicated that DTL requires the presence of hydroxyl groups at the C-3 and C-4 of N-acetyl-d-glucosamine [14].

Other interactions of DTL

• MATERIAL AND METHODS: The TLD consist of LiF powder type DTL 937 read with a PCL 3 automatic TLD reader [15].
• Particularily we analyse those suggested by Nei (IS, DS), Rogers (DR), Reynolds, Weir and Cockerham (D theta), Nei, Tajima and Tateno (DA), Tomiuk and Loeschcke (ITL, DTL) and Goldstein et al [16].
• A novel WD40 domain-containing protein, L2DTL/CDT2 and PCNA were identified as proteins associated with CUL4/DDB1 complexes [17].
• L2DTL protein decreased in mitosis via degradation by the APC/C-Cdh1 complex [12].
• L2DTL gene expression increased during G1/S phase, and the DNA synthesis in liver regeneration [12].

Analytical, diagnostic and therapeutic context of DTL

• Recordings were between DTL fibres placed under contact lenses on the two eyes [18].
• An improved method for using the DTL fibre in electroretinography [19].
• MATERIAL AND METHODS: Two digital archives were used (DTL and MOD) and the capacity of these archives could no longer support the needs of the Medical Imaging Centre. The entire content of the DLT archive and selected data from the MOD archive were transferred to the current higher capacity (17 TB) tape archive [20].
• To compare the recording characteristics of the DTL fiber and Jet contact lens electrodes, ERG responses were recorded from 20 normal subjects using the ISCEV standard clinical protocol [21].
• L2DTL downregulation by RNAi oligos led to reduced cancer cell growth and invasion capability in vitro, in which microarray analysis disclosed dysregulation of genes involved in cell cycle regulation, chromosome segregation, and cell division [12].

References