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Gene Review

TPS1  -  alpha,alpha-trehalose-phosphate synthase...

Saccharomyces cerevisiae S288c

Synonyms: BYP1, CIF1, FDP1, GGS1, GLC6, ...
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Disease relevance of TPS1

  • In contrast, membrane traffic operated many hours after severe heat stress even in the absence of the TPS1 gene, demonstrating that trehalose had no role in thermoprotection of membranes engaged in vesicular traffic [1].
  • Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex [2].
  • Upon induction of tps1, trehalose increased, and this was associated with increased tolerance to anoxia [3].

High impact information on TPS1


Chemical compound and disease context of TPS1

  • However, they also indicate that the yeast TPS enzyme, as opposed to the E. coli otsA gene product, is able to increase the efficiency of the Tre6P control on glucose influx into yeast glycolysis [8].
  • Complementation studies with E. coli galU mutants showed that the TPS1-encoded trehalose-6-phosphate synthase is UDP-glucose-dependent [9].

Biological context of TPS1


Anatomical context of TPS1

  • In permeabilized spheroplasts, hexokinase activity is significantly lower in a tps2Delta strain compared to a wild-type strain and this difference is strongly reduced by additional deletion of TPS1 [15].

Associations of TPS1 with chemical compounds


Physical interactions of TPS1


Regulatory relationships of TPS1

  • Afterwards, the Tps1-dependent control system is apparently able to restrict properly up to 50-fold higher hexokinase activity [20].
  • Furthermore, high-copy MCK1 or heterozygous hxk2 mutations can suppress the tps1 sporulation trait [21].
  • It was observed that the PKC1 pathway was hardly activated in a tps1 mutant that is unable to accumulate any trehalose [22].

Other interactions of TPS1

  • Since we observed measurable trehalose in strains lacking all but the TPS1 gene, some phosphatase activity in addition to Tps2 can hydrolyze trehalose 6-phosphate [16].
  • Our results show that overexpression of hexokinase does not cause the same phenotype as deletion of Tps1 [20].
  • During the initiation of fermentation overexpression of hexokinase PII in yeast transiently causes a similar deregulation of glycolysis as deletion of Tps1 [20].
  • The expression of genes repressed by cAMP, GAC1 and TPS1, fused to beta-galactosidase, was also stimulated by deletion of SSA genes [23].
  • These genes are located on chromosomes II (TSS1) and XIII (TSL1) [24].

Analytical, diagnostic and therapeutic context of TPS1


  1. Trehalose is required for conformational repair of heat-denatured proteins in the yeast endoplasmic reticulum but not for maintenance of membrane traffic functions after severe heat stress. Simola, M., Hänninen, A.L., Stranius, S.M., Makarow, M. Mol. Microbiol. (2000) [Pubmed]
  2. Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex. Kaasen, I., McDougall, J., Strøm, A.R. Gene (1994) [Pubmed]
  3. Role of trehalose phosphate synthase in anoxia tolerance and development in Drosophila melanogaster. Chen, Q., Ma, E., Behar, K.L., Xu, T., Haddad, G.G. J. Biol. Chem. (2002) [Pubmed]
  4. Yeast adapt to near-freezing temperatures by STRE/Msn2,4-dependent induction of trehalose synthesis and certain molecular chaperones. Kandror, O., Bretschneider, N., Kreydin, E., Cavalieri, D., Goldberg, A.L. Mol. Cell (2004) [Pubmed]
  5. The danger of metabolic pathways with turbo design. Teusink, B., Walsh, M.C., van Dam, K., Westerhoff, H.V. Trends Biochem. Sci. (1998) [Pubmed]
  6. Trehalose synthase: guard to the gate of glycolysis in yeast? Thevelein, J.M., Hohmann, S. Trends Biochem. Sci. (1995) [Pubmed]
  7. A yeast homologue of the bovine lens fibre MIP gene family complements the growth defect of a Saccharomyces cerevisiae mutant on fermentable sugars but not its defect in glucose-induced RAS-mediated cAMP signalling. Van Aelst, L., Hohmann, S., Zimmermann, F.K., Jans, A.W., Thevelein, J.M. EMBO J. (1991) [Pubmed]
  8. Expression of escherichia coli otsA in a Saccharomyces cerevisiae tps1 mutant restores trehalose 6-phosphate levels and partly restores growth and fermentation with glucose and control of glucose influx into glycolysis. Bonini, B.M., Van Vaeck, C., Larsson, C., Gustafsson, L., Ma, P., Winderickx, J., Van Dijck, P., Thevelein, J.M. Biochem. J. (2000) [Pubmed]
  9. A yeast gene for trehalose-6-phosphate synthase and its complementation of an Escherichia coli otsA mutant. McDougall, J., Kaasen, I., Strøm, A.R. FEMS Microbiol. Lett. (1993) [Pubmed]
  10. Gts1p stabilizes oscillations in energy metabolism by activating the transcription of TPS1 encoding trehalose-6-phosphate synthase 1 in the yeast Saccharomyces cerevisiae. Xu, Z., Yaguchi, S., Tsurugi, K. Biochem. J. (2004) [Pubmed]
  11. Cloning and characterization of genes encoding trehalose-6-phosphate synthase (TPS1) and trehalose-6-phosphate phosphatase (TPS2) from Zygosaccharomyces rouxii. Kwon, H.B., Yeo, E.T., Hahn, S.E., Bae, S.C., Kim, D.Y., Byun, M.O. FEMS Yeast Res. (2003) [Pubmed]
  12. Gts1p activates SNF1-dependent derepression of HSP104 and TPS1 in the stationary phase of yeast growth. Yaguchi, S., Tsurugi, K. J. Biol. Chem. (2003) [Pubmed]
  13. Involvement of CIF1 (GGS1/TPS1) in osmotic stress response in Saccharomyces cerevisiae. Hazell, B.W., Kletsas, S., Nevalainen, H., Attfield, P.V. FEBS Lett. (1997) [Pubmed]
  14. Evidence that the Saccharomyces cerevisiae CIF1 (GGS1/TPS1) gene modulates heat shock response positively. Hazell, B.W., Nevalainen, H., Attfield, P.V. FEBS Lett. (1995) [Pubmed]
  15. Reconstitution of ethanolic fermentation in permeabilized spheroplasts of wild-type and trehalose-6-phosphate synthase mutants of the yeast Saccharomyces cerevisiae. Noubhani, A., Bunoust, O., Rigoulet, M., Thevelein, J.M. Eur. J. Biochem. (2000) [Pubmed]
  16. Composition and functional analysis of the Saccharomyces cerevisiae trehalose synthase complex. Bell, W., Sun, W., Hohmann, S., Wera, S., Reinders, A., De Virgilio, C., Wiemken, A., Thevelein, J.M. J. Biol. Chem. (1998) [Pubmed]
  17. Fps1, a yeast member of the MIP family of channel proteins, is a facilitator for glycerol uptake and efflux and is inactive under osmotic stress. Luyten, K., Albertyn, J., Skibbe, W.F., Prior, B.A., Ramos, J., Thevelein, J.M., Hohmann, S. EMBO J. (1995) [Pubmed]
  18. Synergy between trehalose and Hsp104 for thermotolerance in Saccharomyces cerevisiae. Elliott, B., Haltiwanger, R.S., Futcher, B. Genetics (1996) [Pubmed]
  19. On the mechanism by which a heat shock induces trehalose accumulation in Saccharomyces cerevisiae. Neves, M.J., François, J. Biochem. J. (1992) [Pubmed]
  20. During the initiation of fermentation overexpression of hexokinase PII in yeast transiently causes a similar deregulation of glycolysis as deletion of Tps1. Ernandes, J.R., De Meirsman, C., Rolland, F., Winderickx, J., de Winde, J., Brandão, R.L., Thevelein, J.M. Yeast (1998) [Pubmed]
  21. Roles of trehalose phosphate synthase in yeast glycogen metabolism and sporulation. De Silva-Udawatta, M.N., Cannon, J.F. Mol. Microbiol. (2001) [Pubmed]
  22. Activation of the protein kinase C1 pathway upon continuous heat stress in Saccharomyces cerevisiae is triggered by an intracellular increase in osmolarity due to trehalose accumulation. Mensonides, F.I., Brul, S., Klis, F.M., Hellingwerf, K.J., Teixeira de Mattos, M.J. Appl. Environ. Microbiol. (2005) [Pubmed]
  23. Ssa1p chaperone interacts with the guanine nucleotide exchange factor of ras Cdc25p and controls the cAMP pathway in Saccharomyces cerevisiae. Geymonat, M., Wang, L., Garreau, H., Jacquet, M. Mol. Microbiol. (1998) [Pubmed]
  24. Cloning of two related genes encoding the 56-kDa and 123-kDa subunits of trehalose synthase from the yeast Saccharomyces cerevisiae. Vuorio, O.E., Kalkkinen, N., Londesborough, J. Eur. J. Biochem. (1993) [Pubmed]
  25. Molecular cloning of CIF1, a yeast gene necessary for growth on glucose. González, M.I., Stucka, R., Blázquez, M.A., Feldmann, H., Gancedo, C. Yeast (1992) [Pubmed]
  26. Trehalose-6-phosphate synthase/phosphatase complex from bakers' yeast: purification of a proteolytically activated form. Londesborough, J., Vuorio, O. J. Gen. Microbiol. (1991) [Pubmed]
  27. Concomitant appearance of intrinsic thermotolerance and storage of trehalose in Saccharomyces cerevisiae during early respiratory phase of batch-culture is CIF1-dependent. Attfield, P.V., Kletsas, S., Hazell, B.W. Microbiology (Reading, Engl.) (1994) [Pubmed]
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