Disease relevance of G10

• Effects of intratracheal administration of novispirin G10 on a rat model of mucoid Pseudomonas aeruginosa lung infection [1].

High impact information on G10

• The G10 antigen is thermolabile and co-migrates with microtubule-associated protein (MAP)1 from young rat brain on low percentage (5%) polyacrylamide-SDS gels [2].
• EDG-2, -4, and -7 bind the ligand lysophosphatidic acid [3].
• At concentrations of 10% or less of that in plasma, which are observed in fluids of injured tissues, purified and recombinant gelsolin augment LPA stimulation of nuclear signals and protein synthesis in rat cardiac myocytes (RCMs) that express Edg-2 and -4 receptors [4].
• We have previously found that adult Schwann cells express receptors for lysophosphatidic acid (EDG2, EDG7) and sphingosine-1-phosphate (EDG5) and that expression of these receptors is significantly upregulated in injured sciatic nerve coincident with postaxotomy Schwann cell proliferation [5].
• RT-PCR and Northern blot analysis indicate the co-expression of mRNAs encoding the EDG-2, EDG-4, and PSP24 receptors in a variety of cell lines and tissues [6].

Biological context of G10

• The phase-angle difference in the circadian rhythm between G10 pups and G17 or S pups suggests that fetal circadian oscillation is entrainable at least after day 10 of gestation [7].
• Enzymatic hydrolysis of the modified initiator tRNA, followed by separation of nucleosides on Sephadex G10 or Dowex 50 columns, revealed that 7-methylguanosine was the principal labelled product [8].
• Pregnant rats (N = 7) were dosed p.o. with O,O,S-trimethyl phosphorothioate (OOS-TMP) (LD50 = 85) at 20 mg/kg on days G8 and G10 [9].

Anatomical context of G10

• The spatial and chronological coincidence of the G10 epitope with axonal elongation offers the possibility of using this monoclonal antibody to probe the function of the cytoskeleton during neuronal growth [10].
• The distribution of the G10 epitope as shown by immuno-electron microscopy is consistent with its localization to axonal microtubules [10].
• 2. Solutes greater than 500 daltons were largely removed by serial ultrafiltrations through molecular sieves (Amicon membranes UM10 and UM05); solutes less than 350 daltons were largely eliminated by gel filtration through Sephadex G10 columns [11].
• In this paper we show that all growing axons investigated in post-natal and pre-natal developing rat brain contain microtubule-associated protein 1x and that neither dendrites, cell bodies nor glia are labelled by G10 [10].
• Monoclonal antibody G10 binds to an epitope on a microtubule-associated protein and specifically labels elongating axons in the developing rat central nervous system [12].

Associations of G10 with chemical compounds

• Rapid concurrent automated fluorimetric assay of noradrenaline, dopamine, 3,4-dihydroxyphenylacetic acid, homovanillic acid and 3-methoxytyramine in milligram amounts of nervous tissue after isolation on Sephadex G10 [13].
• We reinvestigated this controversial issue in a single experimental model by culturing rat islets for 1 wk in 10 or 30 mmol/l glucose (G10, Controls; or G30, High-glucose islets) before testing the effect of stepwise glucose stimulation from G0.5 to G20 on key beta-cell stimulus-secretion coupling events [14].
• Pregnant Wistar rats received an enteral dose of nicotine (2.5, 5.0 or 10mg/kg, respectively, in groups G2, G5, and G10) from the 10th to the 18th or 20th days of pregnancy [15].
• These cells do not appear to be mature T cells, they are partially adherent to plastic, sephadex G10 and nylon wool, insensitive to indomethacin and are enriched in a 1.07 g/ml fraction of a Percoll density gradient [16].

Other interactions of G10

• The method is based on the conversion of DOPA to 3-O-[methyl-3H]DOPA by catechol-O-methyltransferase in the presence of S-adenosyl-[methyl-3H]methionine and purification of the labelled product by Sephadex G10 and Dowex 50 W x 4 ion exchange resin [17].
• Monoclonal antibody G10 against microtubule-associated protein 1x distinguishes between growing and regenerating axons [12].
• Catalase was immunolocalized exclusively in Leydig cell peroxisomes using AuroProbe EM protein A G10 (ie, 10 nm gold particles) [18].
• Twenty-day-old control fetus and of G2 and G5 had a high sarcoplasmic expression of desmin (weaker in G10) [15].

Analytical, diagnostic and therapeutic context of G10

• Immunofluorescence microscopy using MAb G10 on parasagittal sections of rat cerebella shows labelling of the newly formed molecular layer in 6 day old rats [2].
• At 0 and 3 h post P. aeruginosa infection, the treated group of rats received novispirin G10 (0.1 mg/ml, 0.1 ml/rat) intratracheally, whereas the control group received vehicle treatment only [1].
• One highly retarded fraction was obtained (GF-3), which was either used for testing or further purified by filtration on Sephadex G10, the G10-3 fraction of which was used for experiments [19].

References