Psychiatry related information on Prdx6

• Thyrocytes have several defense mechanisms against ROS, including TSA and SOD (superoxide anion dismutase) [1].

High impact information on Prdx6

• Several significant structural differences have been found from a 1-Cys peroxiredoxin, ORF6, which lacks the C-terminal conserved cysteine corresponding to Cys-173 of HBP23 [2].
• An antisense oligonucleotide to 1-cys peroxiredoxin causes lipid peroxidation and apoptosis in lung epithelial cells [3].
• It also showed sequence identity to bacterial alkyl hydroperoxide reductases and thiol specific antioxidant enzymes [4].
• Identification of a 28 kDa secretory protein from rat olfactory epithelium as a thiol-specific antioxidant [5].
• PURPOSE: To investigate 1-cys peroxiredoxin (1-cysPrx) expression during the corneal wound-healing process after PRK and the effect of growth factors on 1-cysPrx expression in cultured bovine keratocytes (BKs) [6].

Biological context of Prdx6

• The amino acid sequence of the 28-kDa protein is similar to that of non-selenium glutathione peroxidase from bovine ciliary body [7].
• Rat pachytene spermatocytes down-regulate a polo-like kinase and up-regulate a thiol-specific antioxidant protein, whereas sertoli cells down-regulate a phosphodiesterase and up-regulate an oxidative stress protein after exposure to methoxyethanol and methoxyacetic acid [8].
• The effects on TSA gene expression were specific to TSH [1].
• Thiol-specific antioxidant (TSA) plays an important role in regulating cell differentiation and proliferation by modulating the hydrogen peroxide (H2O2) mediated responses in a variety of mammalian cells [1].
• The half-life of TSA mRNA was approximately 5.5 hours in the presence or absence of TSH, and that was not affected by TSA mRNA stability [1].

Anatomical context of Prdx6

• Subcellular fractions prepared by differential centrifugation of rat lung homogenates showed highest specific activity of aiPLA2 in the lamellar body and lysosomal fractions, lower activity in cytosol, and essentially no activity in mitochondria, microsomes, or plasma membranes [9].
• The results of this study indicate that aiPLA2 has the major role in the degradation of internalized DPPC by granular pneumocytes and they are compatible with participation of lysosomes/lamellar bodies in DPPC metabolism [9].
• Using Northern blot hybridization, we tested the effects of TSH on TSA gene expression in FRTL-5 cells derived from rat thyroids [1].

Associations of Prdx6 with chemical compounds

• The extent of adduction of each protein was calculated, and two proteins, peroxiredoxin 6 and biliverdin reductase, were adducted at high specific activities (0.36-0.70 and 1.0 nmol adduct/nmol protein) [10].
• Peroxiredoxin-6, glucose-regulated protein 78, zinc-alpha2-glycoprotein, and phospholipase Calpha are also secreted [11].
• Other growth factors (e.g., insulin, transferrin and hydrocortisone) did not alter TSA expression [1].

Other interactions of Prdx6

• When the protein levels of the major selenium-containing glutathione peroxidase, GPX1, and the recently identified non-selenium-containing glutathione peroxidase, peroxiredoxin 6, were evaluated by immunoblotting using specific antibodies, they gradually declined after birth [12].

Analytical, diagnostic and therapeutic context of Prdx6

• Sequence analyses indicate that clones 1 and 6 have 83% and 79% homology at the nucleotide level to a polo-like kinase and a thiol-specific antioxidant, respectively [8].
• Regulation of 1-cys peroxiredoxin expression in the process of stromal wound healing after photorefractive keratectomy [6].
• Patterns of expression of the 2-Cys and 1-Cys peroxiredoxin (Prx) proteins of the rodent malaria parasite Plasmodium yoelii during its life cycle were observed by immunofluorescent antibody staining and confocal laser scanning microscopy [13].
• Western blot of subcellular fractions from rat lung indicated aiPLA2 immunoreactivity with lamellar body, lysosomal, and cytosolic fractions [14].
• A 29 kDa protein that reacted with the aiPLA2 antipeptide antibody was detected in fractions containing aiPLA2 activity on sequential column chromatographies [14].

References