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Gene Review

Prdx6  -  peroxiredoxin 6

Mus musculus

Synonyms: 1-Cys PRX, 1-Cys Prx, 1-Cys peroxiredoxin, 1-cysPrx, 9430088D19Rik, ...
 
 
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Disease relevance of Prdx6

  • In this study, we evaluated whether genetic inactivation of Prdx6 in mice increases sensitivity to oxygen toxicity [1].
  • After 72-h exposure to 100% O(2), lungs of Prdx6-/- mice showed more severe injury than wild-type with increased wet/dry weight, epithelial cell necrosis and alveolar edema on microscopic examination, increased protein and nucleated cells in BALF, and higher content of TBARS and protein carbonyls in lung homogenate [1].
  • Since significant amounts of catalase and GSHPx are present in the heart contributing toward the attenuation of H(2)O(2) and hydroperoxides formed during ischemia-reperfusion injury and thereby providing cardioprotection, we asked whether Prdx6 also has any role in this process [2].
  • Randomly selected hearts from Prdx6(-/-) mice and wild-type mice were subjected to 30 min of global ischemia followed by 120 min of reperfusion at normothermia [2].
  • The hearts from the Prdx6(-/-) mice were more susceptible to ischemic reperfusion injury as evidenced by reduced recovery of left ventricular function, increased myocardial infarct size, and higher amount of apoptotic cardiomyocytes compared with wild-type mouse hearts [2].
 

High impact information on Prdx6

  • Aging MTPa+/- mice had higher antioxidant activity of total SOD and GPx, lower GSH, and increased expression of cytochrome P-450 2E1, consistent with increased hepatic oxidative stress [3].
  • In patients with chronic gastritis and gastric ulcers, the titer of CP3 antibody was significantly higher than in normal controls and correlated with the histological grade of antral gastritis [4].
  • In conclusion, our results suggest that a moderate GPx increase can be sufficient to prevent irreversible functional damage produced by transient hypoxia in the hippocampus and to help maintain basic electrophysiological mechanisms involved in memory formation [5].
  • Proteomic analyses of mouse brains by two-dimensional differential gel electrophoresis showed that several proteins, including growth hormone, 1-Cys peroxiredoxin, CCT-zeta, glucose-6-phosphate isomerase, GRP170 precursor, and alpha-SNAP, were differentially expressed [6].
  • Using targeted inactivation of the Prdx6 gene, we present evidence that the corresponding protein offsets the deleterious effects of ROS on lens epithelial cells (LECs) and regulates gene expression by limiting its levels [7].
 

Chemical compound and disease context of Prdx6

  • Thus, Prdx6 protects lungs against PQ toxicity as shown previously for hyperoxia, indicating that it functions as an important lung antioxidant enzyme [8].
  • Recombinant human NSGPx expressed in Escherichia coli from a human cDNA clone (HA0683) showed GSH peroxidase activity with sn-2-linolenoyl- or sn-2-arachidonoyl-phosphatidylcholine hydroperoxides as substrate; NADPH or thioredoxin could not substitute for GSH [9].
 

Biological context of Prdx6

  • However, overexpression of Prdx6 had no protective effect on LDL oxidation in vitro, and transgenic mice fed an atherogenic diet for 10 weeks did not possess an increased resistance to atherosclerosis nor did they maintain the high prediet plasma HDL levels consistent with the Ath1-resistant phenotype [10].
  • We recently identified Prdx6 as a candidate for the quantitative trait locus Ath1, a gene responsible for a difference in diet-induced atherosclerosis susceptibility in mice [10].
  • These Prdx6(-/-) hearts were also subjected to a higher amount of oxidative stress as evidenced by the presence of higher amount of malondialdehyde [2].
  • Using Northern blotting with various Prdx6 probes, we have revealed the existence of multiple transcripts with distinct tissue distributions and regulation, including the major 1.4-kb transcript highly expressed in liver and lung, and two additional transcripts expressed primarily in liver [11].
  • Mice have a highly related intronless gene (1-cysPrx-P1, GenBank accession number AF085220) with the same length of open reading frame (224 aa) as 1-cysPrx but located on a different chromosome [12].
 

Anatomical context of Prdx6

 

Associations of Prdx6 with chemical compounds

  • Additionally, there were no differences in the mRNA expression levels of other peroxiredoxins, glutathione peroxidases, catalase, superoxide dismutases, thioredoxins, and glutaredoxins between normal Prdx6-/- and Prdx6+/+ mice and those injected with paraquat [13].
  • These findings show that Prdx6 -/- mice have increased sensitivity to hyperoxia and provide in vivo evidence that Prdx6 is an important lung antioxidant enzyme [1].
  • These results confirm an important role for Prdx6 in lung surfactant DPPC degradation and synthesis by the reacylation pathway [17].
  • Degradation of internalized [3H]DPPC in isolated mouse lungs after endotracheal instillation of unilamellar liposomes labeled with [3H]DPPC was significantly decreased at 2 h in Prdx6-/- mice (13.6 +/- 0.3% vs. 26.8 +/- 0.8% in the wild type), reflected by decreased dpm in the lysophosphatidylcholine and the unsaturated PC fractions [17].
  • Plasma lipid hydroperoxide levels were higher in atherogenic diet-fed Prdx6-/- mice with B6;129 and B6 backgrounds than in controls [18].
 

Physical interactions of Prdx6

 

Regulatory relationships of Prdx6

 

Other interactions of Prdx6

 

Analytical, diagnostic and therapeutic context of Prdx6

References

  1. Lung injury and mortality with hyperoxia are increased in peroxiredoxin 6 gene-targeted mice. Wang, Y., Feinstein, S.I., Manevich, Y., Ho, Y.S., Fisher, A.B. Free Radic. Biol. Med. (2004) [Pubmed]
  2. Targeted disruption of peroxiredoxin 6 gene renders the heart vulnerable to ischemia-reperfusion injury. Nagy, N., Malik, G., Fisher, A.B., Das, D.K. Am. J. Physiol. Heart Circ. Physiol. (2006) [Pubmed]
  3. Mice heterozygous for a defect in mitochondrial trifunctional protein develop hepatic steatosis and insulin resistance. Ibdah, J.A., Perlegas, P., Zhao, Y., Angdisen, J., Borgerink, H., Shadoan, M.K., Wagner, J.D., Matern, D., Rinaldo, P., Cline, J.M. Gastroenterology (2005) [Pubmed]
  4. A novel enzyme immunoassay for serodiagnosis of Helicobacter pylori infection. Sugiyama, T., Imai, K., Yoshida, H., Takayama, Y., Yabana, T., Yokota, K., Oguma, K., Yachi, A. Gastroenterology (1991) [Pubmed]
  5. Impairment of synaptic transmission by transient hypoxia in hippocampal slices: improved recovery in glutathione peroxidase transgenic mice. Furling, D., Ghribi, O., Lahsaini, A., Mirault, M.E., Massicotte, G. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  6. Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein. Steinacker, P., Schwarz, P., Reim, K., Brechlin, P., Jahn, O., Kratzin, H., Aitken, A., Wiltfang, J., Aguzzi, A., Bahn, E., Baxter, H.C., Brose, N., Otto, M. Mol. Cell. Biol. (2005) [Pubmed]
  7. Impaired homeostasis and phenotypic abnormalities in Prdx6-/-mice lens epithelial cells by reactive oxygen species: increased expression and activation of TGFbeta. Fatma, N., Kubo, E., Sharma, P., Beier, D.R., Singh, D.P. Cell Death Differ. (2005) [Pubmed]
  8. Peroxiredoxin 6 gene-targeted mice show increased lung injury with paraquat-induced oxidative stress. Wang, Y., Feinstein, S.I., Manevich, Y., Ho, Y.S., Fisher, A.B. Antioxid. Redox Signal. (2006) [Pubmed]
  9. Phospholipid hydroperoxides are substrates for non-selenium glutathione peroxidase. Fisher, A.B., Dodia, C., Manevich, Y., Chen, J.W., Feinstein, S.I. J. Biol. Chem. (1999) [Pubmed]
  10. Overexpression of Prdx6 reduces H2O2 but does not prevent diet-induced atherosclerosis in the aortic root. Phelan, S.A., Wang, X., Wallbrandt, P., Forsman-Semb, K., Paigen, B. Free Radic. Biol. Med. (2003) [Pubmed]
  11. Transcripts associated with Prdx6 (peroxiredoxin 6) and related genes in mouse. Simeone, M., Phelan, S.A. Mamm. Genome (2005) [Pubmed]
  12. 1-Cys peroxiredoxin knock-out mice express mRNA but not protein for a highly related intronless gene. Mo, Y., Feinstein, S.I., Manevich, Y., Zhang, Q., Lu, L., Ho, Y.S., Fisher, A.B. FEBS Lett. (2003) [Pubmed]
  13. Mice with targeted mutation of peroxiredoxin 6 develop normally but are susceptible to oxidative stress. Wang, X., Phelan, S.A., Forsman-Semb, K., Taylor, E.F., Petros, C., Brown, A., Lerner, C.P., Paigen, B. J. Biol. Chem. (2003) [Pubmed]
  14. Dysregulation of stathmin, a microtubule-destabilizing protein, and up-regulation of Hsp25, Hsp27, and the antioxidant peroxiredoxin 6 in a mouse model of familial amyotrophic lateral sclerosis. Strey, C.W., Spellman, D., Stieber, A., Gonatas, J.O., Wang, X., Lambris, J.D., Gonatas, N.K. Am. J. Pathol. (2004) [Pubmed]
  15. Induction of 1-cys peroxiredoxin expression by oxidative stress in lung epithelial cells. Kim, H.S., Manevich, Y., Feinstein, S.I., Pak, J.H., Ho, Y.S., Fisher, A.B. Am. J. Physiol. Lung Cell Mol. Physiol. (2003) [Pubmed]
  16. Protective Effects of Ethanolic Neem Leaf Extract on DMBA-Induced Genotoxicity and Oxidative Stress in Mice. Subapriya, R., Kumaraguruparan, R., Abraham, S.K., Nagini, S. Journal of herbal pharmacotherapy. (2005) [Pubmed]
  17. Altered lung phospholipid metabolism in mice with targeted deletion of lysosomal-type phospholipase A2. Fisher, A.B., Dodia, C., Feinstein, S.I., Ho, Y.S. J. Lipid Res. (2005) [Pubmed]
  18. Peroxiredoxin 6 deficiency and atherosclerosis susceptibility in mice: significance of genetic background for assessing atherosclerosis. Wang, X., Phelan, S.A., Petros, C., Taylor, E.F., Ledinski, G., Jürgens, G., Forsman-Semb, K., Paigen, B. Atherosclerosis (2004) [Pubmed]
  19. Characterization of the murine gene encoding 1-Cys peroxiredoxin and identification of highly homologous genes. Lee, T.H., Yu, S.L., Kim, S.U., Kim, Y.M., Choi, I., Kang, S.W., Rhee, S.G., Yu, D.Y. Gene (1999) [Pubmed]
  20. A novel type of glutathione peroxidase: expression and regulation during wound repair. Munz, B., Frank, S., Hübner, G., Olsen, E., Werner, S. Biochem. J. (1997) [Pubmed]
  21. The gene for the ubiquitous octamer-binding protein Oct-1 is on human chromosome 1, region cen-q32, and near Ly-22 and Ltw-4 on mouse chromosome 1. Hsieh, C.L., Sturm, R., Herr, W., Francke, U. Genomics (1990) [Pubmed]
 
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