Disease relevance of Tritrichomonas

• Expression plasmids encoding the hypoxanthine phosphoribosyltransferases (HPRTs) of Plasmodium falciparum, Schistosoma mansoni, Tritrichomonas foetus, and Homo sapiens were subcloned into genetically deficient Escherichia coli that requires complementation by the activity of a recombinant HPRT for growth on semidefined medium [1].
• The substituted benzimidazole omeprazole, used for the treatment of human peptic ulcer disease, inhibits the growth of the metronidazole-resistant bovine pathogen Tritrichomonas foetus in vitro (MIC at which the growth of parasite cultures is inhibited by 50%, 22 microg/ml [63 microM]) [2].

High impact information on Tritrichomonas

• Pyrimidine metabolism in Tritrichomonas foetus [3].
• The anaerobic parasitic protozoa Tritrichomonas foetus is found incapable of de novo pyrimidine biosynthesis by its failure to incorporate bicarbonate, aspartate, or orotate into pyrimidine nucleotides or nucleic acids [3].
• The high resolution crystal structures of the protozoan parasite Tritrichomonas foetus IMPDH complexed with the inhibitor ribavirin monophosphate as well as monophosphate together with a second inhibitor, mycophenolic acid, are presented here [4].
• Crystal structure of Tritrichomonas foetus inosine monophosphate dehydrogenase in complex with the inhibitor ribavirin monophosphate reveals a catalysis-dependent ion-binding site [4].
• Aerobic incubations of the Tritrichomonas foetus hydrogenosomal fraction containing pyruvate, CoA, and the spin trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) gave spectra of two radical adducts [5].

Biological context of Tritrichomonas

• Altering the purine specificity of hypoxanthine-guanine-xanthine phosphoribosyltransferase from Tritrichomonas foetus by structure-based point mutations in the enzyme protein [6].
• Trichomonad parasites such as Tritrichomonas foetus produce large amounts of putrescine (1,4-diaminobutane), which is transported out of the cell via an antiport mechanism which results in the uptake of a molecule of spermine [7].
• Hydrogen peroxide induces caspase activation and programmed cell death in the amitochondrial Tritrichomonas foetus [8].
• Immunolocalization of tubulin isoforms and post-translational modifications in the protists Tritrichomonas foetus and Trichomonas vaginalis [9].

Anatomical context of Tritrichomonas

• Identification of a neutrophil chemotactic factor from Tritrichomonas foetus as superoxide dismutase [10].
• The polyene antibiotic, filipin, was used as a probe for the detection of sterols in the freeze-fractured plasma membrane and the flagellar membranes of the pathogenic protozoa, Tritrichomonas foetus [11].
• We used monoclonal antibodies specific for acetylated and nonacetylated alpha-tubulin to detect and to localize microtubules containing acetylated alpha-tubulin (stable microtubules) in the pathogenic protozoa Tritrichomonas foetus and Trichomonas vaginalis [12].

Associations of Tritrichomonas with chemical compounds

• But DNA fragments of a metronidazole-sensitive Tritrichomonas foetus hybridized specifically with the double-stranded RNA, even though this organism does not contain the double-stranded RNA itself [13].
• Hydrogenosomes of the anaerobic flagellate Tritrichomonas foetus can use oxygen as terminal electron acceptor in pyruvate metabolism [14].
• Respiration of hydrogenosomes of Tritrichomonas foetus. II. Effect of CoA on pyruvate oxidation [14].
• To explore this idea, we attempted to block the purine salvage pathway of the parasitic protozoan Tritrichomonas foetus [15].
• Purification and characterization of a sialic acid-specific lectin from Tritrichomonas mobilensis [16].

Gene context of Tritrichomonas

• Finally, T. vaginalis and Tritrichomonas foetus but not Pentatrichomonas hominis induce expression of COX-2 [17].
• Hydrogenosomal ferredoxin of the anaerobic protozoon, Tritrichomonas foetus [18].
• The cloning and characterization of Ts-p66, a calcium-binding protein representing calnexin of the protozoan parasite Tritrichomonas suis is described [19].
• Lactoferrin-binding proteins of Tritrichomonas foetus [20].
• Tritrichomonas foetus: iron acquisition from lactoferrin and transferrin [21].

Analytical, diagnostic and therapeutic context of Tritrichomonas

• In the present report we show the distribution of multiple tubulin isoforms in Trichomonas vaginalis and Tritrichomonas foetus, flagellated parasitic protists of the urogenital tracts of human and cattle, respectively, using immunofluorescence and immunoelectron microscopy [9].
• Use of DMSO for the cryopreservation of Tritrichomonas foetus in liquid nitrogen [22].
• The objectives of this study were to determine the detection limit of a PCR test for Tritrichomonas foetus, to investigate the effect of sampling method, guanidinium thiocyanate (GuSCN), and sample storage, and to confirm the accuracy of the test on field samples [23].
• Electron spectroscopic imaging (ESI) of very thin unstained sections of Tritrichomonas foetus fixed in an osmium tetroxide solution containing calcium and potassium ferrocyanide showed the presence of calcium in the form of electron-dense deposits located in the hydrogenosomes [24].

References