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Chemical Compound Review

AC1L3XCG     (2,5-dioxopyrrolidin-1-yl) 3-(4-hydroxy-3...

Synonyms: 65114-37-6
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Disease relevance of Bolton-Hunter reagent


High impact information on Bolton-Hunter reagent

  • Acylated ubiquitin could not be radiolabeled with a membrane-impermeable Bolton-Hunter reagent unless virus was pretreated with detergent [5].
  • Purified human IL-1 was labeled to high specific activity with 125I, using Bolton-Hunter reagent [6].
  • CSVTCG derivatized with [125I] Bolton-Hunter reagent was taken up by cells in a dose-dependent manner and the cell association was inhibited with a monospecific polyclonal anti-CBP antibody [7].
  • Highly purified eIF-2 from rabbit reticulocytes was labeled with 125I by using the Bolton-Hunter reagent or with [gamma-32P]ATP by using the heme-regulated translational inhibitor protein kinase [8].
  • Unlike the case with the chloramine T method, incorporation of a single mole of Bolton-Hunter reagent into a mole of IFN-beta Ser17 led to nearly complete loss of biological activity [9].

Biological context of Bolton-Hunter reagent

  • A conjugate (125I-BH-SP) between substance P and 125I-labeled Bolton-Hunter reagent binds reversibly to a single class of high affinity (Kd = 4 nM) binding sites on dispersed rat parotid cells [10].
  • A human lung tumor-associated antigen, previously purified to apparent homogeneity from an extract of a small cell tumor, was radioiodinated with Bolton-Hunter reagent for use in a competitive protein-binding radioimmunoassay [11].
  • Experiments designed (1) to detect structures at the 5' ends of phosphatase-treated nascent DNA molecules that caused them to be resistant to hydrolysis by spleen exonuclease or (2) to detect polypeptides that were associated covalently with small DNA molecules and could be iodinated with the Bolton-Hunter reagent did not yield positive results [12].
  • A radiolabeled analogue of neuromedin N has been prepared by acylation of the N-terminal amino group of the peptide with the 125I-labeled Bolton-Hunter reagent [13].
  • 1-Acyl-2-[[[3-(3-[125I]iodo-4-hydroxyphenyl)- propionyl]amino]caproyl]phosphatidylcholine (125I-PC) was prepared by alkylation of 1-acyl-2-(aminocaproyl)phosphatidylcholine with monoiodinated Bolton-Hunter reagent [14].

Anatomical context of Bolton-Hunter reagent


Associations of Bolton-Hunter reagent with other chemical compounds


Gene context of Bolton-Hunter reagent


Analytical, diagnostic and therapeutic context of Bolton-Hunter reagent


  1. Isolation of Mycoplasma pulmonis membranes and identification of surface antigens. Horowitz, S.A., Garrett, B., Davis, J.K., Cassell, G.H. Infect. Immun. (1987) [Pubmed]
  2. Penicillin-binding proteins and peptidoglycan of Treponema pallidum subsp. pallidum. Radolf, J.D., Moomaw, C., Slaughter, C.A., Norgard, M.V. Infect. Immun. (1989) [Pubmed]
  3. Effect of radioiodination on the binding of monoclonal antibody DF3 to breast carcinoma cells. Hayes, D.F., Noska, M.A., Kufe, D.W., Zalutsky, M.R. International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology. (1988) [Pubmed]
  4. Characterization of relaxin radioimmunoassay using Bolton-Hunter reagent. First results in plasma during pregnancy, and in placenta, corpora lutea and ovarian cysts in woman. Loumaye, E., Teuwissen, B., Thomas, K. Gynecol. Obstet. Invest. (1978) [Pubmed]
  5. Ubiquitin is attached to membranes of baculovirus particles by a novel type of phospholipid anchor. Guarino, L.A., Smith, G., Dong, W. Cell (1995) [Pubmed]
  6. Detection and characterization of high affinity plasma membrane receptors for human interleukin 1. Dower, S.K., Kronheim, S.R., March, C.J., Conlon, P.J., Hopp, T.P., Gillis, S., Urdal, D.L. J. Exp. Med. (1985) [Pubmed]
  7. Identification and characterization of a tumor cell receptor for CSVTCG, a thrombospondin adhesive domain. Tuszynski, G.P., Rothman, V.L., Papale, M., Hamilton, B.K., Eyal, J. J. Cell Biol. (1993) [Pubmed]
  8. Binding and release of eukaryotic initiation factor eIF-2 and GTP during protein synthesis initiation. Trachsel, H., Staehelin, T. Proc. Natl. Acad. Sci. U.S.A. (1978) [Pubmed]
  9. Binding of 125I-labeled recombinant beta interferon (IFN-beta Ser17) to human cells. O'Rourke, E.C., Drummond, R.J., Creasey, A.A. Mol. Cell. Biol. (1984) [Pubmed]
  10. Substance P receptor on parotid cell membranes. Liang, T., Cascieri, M.A. J. Neurosci. (1981) [Pubmed]
  11. Serum levels of a human lung tumor-associated antigen using an improved radioimmunoassay. Braatz, J.A., Hua, D.T., Princler, G.L. Cancer Res. (1983) [Pubmed]
  12. Characteristics of the nascent and non-nascent small DNA molecules found in Escherichia coli. Denhardt, D.T., Miyamoto, C. J. Mol. Biol. (1983) [Pubmed]
  13. Compared binding properties of 125I-labeled analogues of neurotensin and neuromedin N in rat and mouse brain. Gaudriault, G., Zsürger, N., Vincent, J.P. J. Neurochem. (1994) [Pubmed]
  14. Synthesis and properties of radioiodinated phospholipid analogues that spontaneously undergo vesicle-vesicle and vesicle-cell transfer. Schroit, A.J., Madsen, J.W. Biochemistry (1983) [Pubmed]
  15. Molecular mapping of pulmonary endothelial membrane glycoproteins of the intact rabbit lung. Merker, M.P., Carley, W.W., Gillis, C.N. FASEB J. (1990) [Pubmed]
  16. Characterization of the substance P receptor in rat brain cortex membranes and the inhibition of radioligand binding by guanine nucleotides. Cascieri, M.A., Liang, T. J. Biol. Chem. (1983) [Pubmed]
  17. Neuropeptide Y. Differential binding to rat intestinal laterobasal membranes. Nguyen, T.D., Heintz, G.G., Kaiser, L.M., Staley, C.A., Taylor, I.L. J. Biol. Chem. (1990) [Pubmed]
  18. Studies on the fate of receptor-bound 125I-interleukin 1 beta in porcine synovial fibroblasts. Bird, T.A., Saklatvala, J. J. Immunol. (1987) [Pubmed]
  19. The origin of the mesoderm in an anuran, Xenopus laevis, and a urodele, Ambystoma mexicanum. Smith, J.C., Malacinski, G.M. Dev. Biol. (1983) [Pubmed]
  20. Purification and characterization of the D2-dopamine receptor from bovine anterior pituitary. Senogles, S.E., Amlaiky, N., Falardeau, P., Caron, M.G. J. Biol. Chem. (1988) [Pubmed]
  21. Identification and initial characterization of insulin receptor-like immunoreactivity in human plasma. Pezzino, V., Papa, V., Costantino, A., Frittitta, L., Russo, P., Goldfine, I.D., Vigneri, R. J. Clin. Endocrinol. Metab. (1992) [Pubmed]
  22. Regulation of substance P receptor affinity by guanine nucleotide-binding proteins. Macdonald, S.G., Boyd, N.D. J. Neurochem. (1989) [Pubmed]
  23. Binding of a Bolton-Hunter substituted homostatine analog to affinity-immobilized human renin. Evenou, J.P., Weidmann, B., Hagenbach, A., Metternich, R., Pfenninger, E., Wagner, H. Biochem. Pharmacol. (1990) [Pubmed]
  24. Characterization of antisera to cholecystokinin by use of different cholecystokinin labels. Jansen, J.B., Lamers, C.B. J. Immunol. Methods (1984) [Pubmed]
  25. Regulation of cell-surface receptors for human interferon-gamma on the human histiocytic lymphoma cell line U937. Finbloom, D.S. Biochem. J. (1991) [Pubmed]
  26. Radiolabeled ligands specific for the G protein-coupled state of neurotensin receptors. Gaudriault, G., Zsürger, N., Vincent, J.P. J. Neurochem. (1996) [Pubmed]
  27. Radioiodination of transforming growth factor-beta (TGF-beta) in a modified Bolton-Hunter reaction system. Ross, J., Janero, D.R., Hreniuk, D., Wennogle, L.P. J. Biochem. Biophys. Methods (1993) [Pubmed]
  28. Radioimmunoassay of human sex hormone binding globulin: improved radioiodination procedure. Fernlund, P., Gershagen, S., Larsson, I., Rannevik, G. Scand. J. Clin. Lab. Invest. (1985) [Pubmed]
  29. Purification and characterization of a protein associated with peripheral-type benzodiazepine binding sites. Antkiewicz-Michaluk, L., Mukhin, A.G., Guidotti, A., Krueger, K.E. J. Biol. Chem. (1988) [Pubmed]
  30. Overexpression of native human beta 2-microglobulin in Escherichia coli and its purification. Parker, K.C., Wiley, D.C. Gene (1989) [Pubmed]
  31. Isolation and characterization of surface antigens from Schistosoma mansoni. I. Evaluation of techniques for radioisotope labeling of surface proteins from adult worms. Hayunga, E.G., Murrell, K.D., Taylor, D.W., Vannier, W.E. J. Parasitol. (1979) [Pubmed]
  32. Expression of lectin-binding surface glycoproteins during the development of Schistosoma mansoni schistosomula. Hayunga, E.G., Sumner, M.P. J. Parasitol. (1986) [Pubmed]
  33. Alteration of the pharmacokinetics of small proteins by iodination. Bauer, R.J., Leigh, S.D., Birr, C.A., Bernhard, S.L., Fang, M., Der, K., Ihejeto, N.O., Carroll, S.F., Kung, A.H. Biopharmaceutics & drug disposition. (1996) [Pubmed]
  34. Determination of terminal sugars in transferrin by radio-lectin immunoassay (RLIA)-a new microanalytical procedure. Cervén, C., Stibler, H., Borg, S. Ups. J. Med. Sci. (1981) [Pubmed]
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