Disease relevance of phosphinothricin

• The argE gene product of Escherichia coli, representing a N-acetyl-L-ornithine deacetylase was identified to remove the acetyl-group from N-ac-Pt giving the cytotoxic compound L-phosphinothricin (Pt, glufosinate) [1].
• The new plasmid pBAT1 can be positively selected in vitro within Salmonella serovars in the presence of the herbicide DL-phosphinothricin [2].
• From the analysis of clinical symptoms of previously published cases, glufosinate toxicity appears to arise both from the active ingredient and the surfactant in the formulation [3].
• Neurological effects of glufosinate poisoning with a brief review [3].
• A case of transient diabetes insipidus associated with poisoning by a herbicide containing glufosinate [4].

Psychiatry related information on phosphinothricin

• Anterograde amnesia with hippocampal lesions following glufosinate intoxication [5].

High impact information on phosphinothricin

• Among 19 BC1S1 individuals one plant was transgenic, had 43 chromosomes, contained the bar gene, and survived glufosinate treatments [6].
• (creeping bentgrass), which assessed gene flow from transgenic plants resistant to the herbicide glufosinate to surrounding non-transgenic plants [7].
• Hygromycin susceptible, ammonium glufosinate tolerant phenotypes represented 14.4, 17.4 and 14.3% of the plants in T1 progenies of ZZ321, Ariete and KDML105 primary transformants, respectively [8].
• These observations demonstrate that glufosinate specifically induced apoptosis in the neuroepithelium of embryos [9].
• The present study examined the cell death induced specifically by glufosinate in the neuroepithelium of mouse embryos [9].

Chemical compound and disease context of phosphinothricin

• Similarly, bar and pat genes isolated from Streptomyces hygroscopicus and S. viridochromogenes, respectively, have been inserted into plants to encode phosphinothricin N-acetyltransferase to detoxify glufosinate [10].
• Therefore, we optimized both Agrobacterium infection and glufosinate selection in the presence of L-cysteine for Williams 82 [11].

Biological context of phosphinothricin

• Glufosinate-tolerant crops have been commercialized using one of these two transgenes [10].
• Protein binding of glufosinate and factors affecting it revealed by an equilibrium dialysis technique [12].
• We studied the toxicokinetics of glufosinate in a patient with this poisoning successfully treated without extracorporeal hemopurification [13].
• CONCLUSIONS: This research indicates a possible risk of congenital malformations for paternal exposure to some pesticides, notably, pyridils, aliphatic hydrocarbons, inorganic compounds, and glufosinate [14].
• After mutagenesis, surviving plants were exposed to glufosinate to check for herbicide resistance, which was examined up to the second generation [15].

Anatomical context of phosphinothricin

• These effects are suspected to be due to the effects of glufosinate on the central nervous system [4].
• In micromass culture, glufosinate inhibited the differentiation of midbrain cells in day 12 embryos with 50% inhibition occurring at 0.55 microgram/ml (2.8 x 10(-6) M) [16].
• In addition to causing growth retardation, glufosinate specifically affected the neuroepithelium of the brain vesicle and neural tube, leading to neuroepithelial cell death [16].
• In day 10 embryos cultured for 24 hr, glufosinate significantly reduced the crown-rump length and the number of somite pairs, and produced a high incidence of morphological defects (84.6%) at 10 micrograms/ml [16].

Associations of phosphinothricin with other chemical compounds

• Simultaneous quantification of glyphosate, glufosinate, and their major metabolites in rice and soybean sprouts by gas chromatography with pulsed flame photometric detector [17].
• Glufosinate inhibits glutamine synthetase and blocks biosynthesis of glutamine [10].
• Selection for transgenic plants is achieved using the bar gene conferring resistance to glufosinate or nptII gene for resistance to kanamycin [18].
• Relative to other cultivars, common bermudagrass was intermediate in its response to clethodim and among the most tolerant cultivars to glufosinate and glyphosate [19].

Analytical, diagnostic and therapeutic context of phosphinothricin

• Quantitative determination of glufosinate in biological samples by liquid chromatography with ultraviolet detection after p-nitrobenzoyl derivatization [20].
• A comparative investigation of the metabolism of the herbicide glufosinate in cell cultures of transgenic glufosinate-resistant and non-transgenic oilseed rape (Brassica napus) and corn (Zea mays) [21].

References