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Chemical Compound Review

SureCN52720     2-(4,5-dimethyl-1,3-thiazol- 2-yl)-3,5...

Synonyms: SureCN2619261, MolMap_000036, CHEBI:53238, CPD-12133, ZINC13510840, ...
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Disease relevance of Methylthiazoletetrazolium


High impact information on Methylthiazoletetrazolium

  • The effect of UCN-01 on the cytotoxicity of gamma irradiation in CA46 and HT-29 cells was determined by use of MTT (thiazolyl blue) and clonogenic (colony-forming) assays, respectively; a clonogenic assay was also used to measure the effect of UCN-01 on the cytotoxicity of cisplatin in transfected and nontransfected MCF-7 cells [4].
  • METHODS: Effects of LPA on intestinal epithelial restitution and proliferation were assessed by using an in vitro wounding model with confluent intestinal epithelial cell line 6 (IEC-6) monolayers and colorimetric thiazolyl blue (MTT) assays [5].
  • Cell viability was analyzed by methylthiazoletetrazolium assay; cell viability was reduced to 60.8 +/- 2.7% of control after stimulation with 300 ng/ml TNF-alpha [6].
  • Cell viability as measured by thiazolyl blue and trypan blue was not altered by BAPTA [7].
  • Compared with vector controls, overexpressing clones were found to be substantially more resistant to ChOOH-induced killing, as determined by annexin-V (early apoptotic) and thiazolyl blue (mitochondrial dehydrogenase) reactivity [8].

Biological context of Methylthiazoletetrazolium


Anatomical context of Methylthiazoletetrazolium

  • In all assays, there was a large reduction (3.5-16.1-fold) in the cytotoxicity of dehydrocrotonin in hepatocytes when complexed with beta-cyclodextrin, whereas for V79 cells the decrease in cytotoxicity was 1.7- and 1.87-fold for MTT reduction and nucleic acid content assays, respectively [14].
  • METHODS: We investigated the effects of beta-HIVS on cultured ovarian endometriotic cyst stromal cells (ECSC) by a modified methylthiazoletetrazolium (MTT) assay, a 5-bromo-2'-deoxyuridine (BrdU) incorporation assay and internucleosomal DNA fragmentation assays [15].
  • In studies of cultured cells, the related dye methylthiazoletetrazolium (MTT) has commonly been used as an indicator of the presence and number of viable cells; that is cells that are capable of producing energy via the TCA cycle [16].
  • This early cytotoxic effect of PIPER on cultured HeLa cells was confirmed by a spectrophotometric/colorimetric method employing methylthiazoletetrazolium (Mossmann assay) [17].
  • Control oocytes were kept at room temperature at 22 degrees C. Oocyte viability was assessed using three different methods: trypan blue staining (TB), thiazolyl blue tetrazolium bromide (MTT) staining and observation of germinal vesicle breakdown (GVBD) [18].

Associations of Methylthiazoletetrazolium with other chemical compounds


Gene context of Methylthiazoletetrazolium

  • HRECs were exposed to varying concentrations of human recombinant IGFBP-3, and growth inhibition was evaluated by thiazolyl blue (MTT) conversion [23].
  • IGFBP-2 secreting clones displayed reduced conversion of thiazolyl blue when compared to negative clones or non-transfected parental 293 cells (P < 0.01) [24].
  • BE-NQ7 cells expressed the highest level of NQO1 and were more susceptible [determined by the thiazolyl blue (MTT) assay] to known antitumor quinones and newer clinical candidates [25].
  • The mitogenic activity of the purified nhaFGF was decreased dramatically comparable to that of the wild-type aFGF (haFGF) detected by methylthiazoletetrazolium method [26].
  • Upon lowering the potassium (K(+)) concentration in medium from 25 to 5 mM (low K(+)), aconitase was inactivated in accordance with the decrease in methylthiazoletetrazolium (MTT)-reducing activity although its mRNA expression did not change [27].

Analytical, diagnostic and therapeutic context of Methylthiazoletetrazolium


  1. Phase I trial of dihydrolenperone in lung cancer patients: a novel compound with in vitro activity against lung cancer. Johnson, B.E., Parker, R., Tsai, C.M., Baltz, J., Miller, M.J., Shoemaker, R., Phelps, R., Bastian, A., Stocker, J., Phares, J. Investigational new drugs. (1993) [Pubmed]
  2. Indomethacin suppresses growth of colon cancer via inhibition of angiogenesis in vivo. Wang, H.M., Zhang, G.Y. World J. Gastroenterol. (2005) [Pubmed]
  3. Nicotinamide adenine dinucleotide -- independent formate dehydrogenase in Mycobacterium phlei. Deyhle, R.R., Barton, L.L. Can. J. Microbiol. (1977) [Pubmed]
  4. UCN-01: a potent abrogator of G2 checkpoint function in cancer cells with disrupted p53. Wang, Q., Fan, S., Eastman, A., Worland, P.J., Sausville, E.A., O'Connor, P.M. J. Natl. Cancer Inst. (1996) [Pubmed]
  5. Modulation of intestinal epithelial wound healing in vitro and in vivo by lysophosphatidic acid. Sturm, A., Sudermann, T., Schulte, K.M., Goebell, H., Dignass, A.U. Gastroenterology (1999) [Pubmed]
  6. C-peptide signals via Galpha i to protect against TNF-alpha-mediated apoptosis of opossum kidney proximal tubular cells. Al-Rasheed, N.M., Willars, G.B., Brunskill, N.J. J. Am. Soc. Nephrol. (2006) [Pubmed]
  7. Chelation of intracellular calcium prevents mesangial cell proliferative responsiveness. Whiteside, C., Munk, S., Zhou, X., Miralem, T., Templeton, D.M. J. Am. Soc. Nephrol. (1998) [Pubmed]
  8. Hyperresistance to cholesterol hydroperoxide-induced peroxidative injury and apoptotic death in a tumor cell line that overexpresses glutathione peroxidase isotype-4. Hurst, R., Korytowski, W., Kriska, T., Esworthy, R.S., Chu, F.F., Girotti, A.W. Free Radic. Biol. Med. (2001) [Pubmed]
  9. A nontoxic diphtheria toxin analogue inhibits neonatal bladder smooth muscle cell proliferation. Kaefer, M., Vemulapalli, S., Freeman, M.R. J. Urol. (2000) [Pubmed]
  10. Cisplatin enhances apoptosis induced by a tumor-selective adenovirus expressing tumor necrosis factor-related apoptosis-inducing ligand. Reddy, R.M., Tsai, W.S., Ziauddin, M.F., Zuo, J., Cole, G.W., Maxhimer, J.B., Fang, B., Schrump, D.S., Nguyen, D.M. J. Thorac. Cardiovasc. Surg. (2004) [Pubmed]
  11. In situ screening assay for cell viability using a dimeric cyanine nucleic acid stain. Becker, B., Clapper, J., Harkins, K.R., Olson, J.A. Anal. Biochem. (1994) [Pubmed]
  12. Effects of exogenous zinc supplementation on intestinal epithelial repair in vitro. Cario, E., Jung, S., Harder D'Heureuse, J., Schulte, C., Sturm, A., Wiedenmann, B., Goebell, H., Dignass, A.U. Eur. J. Clin. Invest. (2000) [Pubmed]
  13. Genotoxicity of goniothalamin in CHO cell line. Umar-Tsafe, N., Mohamed-Said, M.S., Rosli, R., Din, L.B., Lai, L.C. Mutat. Res. (2004) [Pubmed]
  14. Dehydrocrotonin and its beta-cyclodextrin complex: cytotoxicity in V79 fibroblasts and rat cultured hepatocytes. Corrêa, D.H., Melo, P.S., de Carvalho, C.A., de Azevedo, M.B., Durán, N., Haun, M. Eur. J. Pharmacol. (2005) [Pubmed]
  15. beta-Hydroxyisovalerylshikonin induces apoptosis and G0/G1 cell-cycle arrest of endometriotic stromal cells: a preliminary in vitro study. Nishida, M., Nasu, K., Ueda, T., Yuge, A., Takai, N., Narahara, H. Hum. Reprod. (2006) [Pubmed]
  16. 3-nitropropionic acid inhibition of succinate dehydrogenase (complex II) activity in cultured Chinese hamster ovary cells: antagonism by L-carnitine. Scallet, A.C., Haley, R.L., Scallet, D.M., Duhart, H.M., Binienda, Z.K. Ann. N. Y. Acad. Sci. (2003) [Pubmed]
  17. The diimide drug PIPER has a cytotoxic dose-dependent effect in vitro and inhibits telomere elongation in HELA cells. Pillich, R.T., Scarsella, G., Galati, G., Izzo, L., Iacoangeli, A., Castelli, M., Risuleo, G. Anticancer Res. (2005) [Pubmed]
  18. Studies on chilling sensitivity of zebrafish (Danio rerio) oocytes. Isayeva, A., Zhang, T., Rawson, D.M. Cryobiology (2004) [Pubmed]
  19. Hyperresistance to photosensitized lipid peroxidation and apoptotic killing in 5-aminolevulinate-treated tumor cells overexpressing mitochondrial GPX4. Kriska, T., Korytowski, W., Girotti, A.W. Free Radic. Biol. Med. (2002) [Pubmed]
  20. Lethal damage to endothelial cells by oxidized low density lipoprotein: role of selenoperoxidases in cytoprotection against lipid hydroperoxide- and iron-mediated reactions. Thomas, J.P., Geiger, P.G., Girotti, A.W. J. Lipid Res. (1993) [Pubmed]
  21. PKC-epsilon is upstream and PKC-alpha is downstream of mitoKATP channels in the signal transduction pathway of ischemic preconditioning of human myocardium. Hassouna, A., Matata, B.M., Galiñanes, M. Am. J. Physiol., Cell Physiol. (2004) [Pubmed]
  22. Expression of somatostatin receptor subtypes and growth inhibition in human exocrine pancreatic cancers. Kikutsuji, T., Harada, M., Tashiro, S., Ii, S., Moritani, M., Yamaoka, T., Itakura, M. Journal of hepato-biliary-pancreatic surgery. (2000) [Pubmed]
  23. Expression of IGFBP-3 by human retinal endothelial cell cultures: IGFBP-3 involvement in growth inhibition and apoptosis. Spoerri, P.E., Caballero, S., Wilson, S.H., Shaw, L.C., Grant, M.B. Invest. Ophthalmol. Vis. Sci. (2003) [Pubmed]
  24. Insulin-like growth factor-binding protein-2 inhibits proliferation of human embryonic kidney fibroblasts and of IGF-responsive colon carcinoma cell lines. Höflich, A., Lahm, H., Blum, W., Kolb, H., Wolf, E. FEBS Lett. (1998) [Pubmed]
  25. Relationship between NAD(P)H:quinone oxidoreductase 1 (NQO1) levels in a series of stably transfected cell lines and susceptibility to antitumor quinones. Winski, S.L., Swann, E., Hargreaves, R.H., Dehn, D.L., Butler, J., Moody, C.J., Ross, D. Biochem. Pharmacol. (2001) [Pubmed]
  26. High-level expression and purification of a nonmitogenic form of human acidic fibroblast growth factor in Escherichia coli. Wu, X., Su, Z., Li, X., Zheng, Q., Huang, Y., Yuan, H. Protein Expr. Purif. (2005) [Pubmed]
  27. Inactivation of aconitase during the apoptosis of mouse cerebellar granule neurons induced by a deprivation of membrane depolarization. Tabuchi, A., Funaji, K., Nakatsubo, J., Fukuchi, M., Tsuchiya, T., Tsuda, M. J. Neurosci. Res. (2003) [Pubmed]
  28. Differential IGF-independent effects of insulin-like growth factor binding proteins (1-6) on apoptosis of breast epithelial cells. Perks, C.M., Bowen, S., Gill, Z.P., Newcomb, P.V., Holly, J.M. J. Cell. Biochem. (1999) [Pubmed]
  29. Sulfur mustard toxicity in macrophages: effect of dexamethasone. Amir, A., Chapman, S., Kadar, T., Gozes, Y., Sahar, R., Allon, N. Journal of applied toxicology : JAT. (2000) [Pubmed]
  30. Overexpression of Bcl-2 partly inhibits apoptosis of human cervical cancer SiHa cells induced by arsenic trioxide. Deng, Y., Lin, C., Zheng, J., Fu, M., Liang, X., Chen, J., Xiao, P., Wu, M. Chin. Med. J. (2000) [Pubmed]
  31. The neuropeptide FMRFamide can protect cells against apoptosis in the snail digestive gland. Roszer, T., Kappelmayer, J., Nagy, G.G., Szentmiklósi, A.J., Basnakian, A.G., Bánfalvi, G. Apoptosis (2006) [Pubmed]
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