The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

Cd4  -  CD4 antigen

Mus musculus

Synonyms: L3T4, Ly-4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Cd4

  • Role of L3T4 antigen in the activation of various functions of Lyt-1+2- T cells against vaccinia virus [1].
  • A subset of T cell receptors associated with L3T4 molecules mediates C6VL leukemia cell binding of its cognate retrovirus [2].
  • To address this issue, we have studied alpha/beta T-cell antigen receptor gene and protein expression on normal thymocyte subsets of AKR/J mice, as well as on a panel of AKR/J primary thymic lymphomas characterized for CD4 (L3T4) and CD8 (Lyt-2) differentiation antigen expression [3].
  • Protection was determined by comparing the number of viable splenic Listeria in naive mice and in recipients of 60 million CD8-enriched, L3T4-depleted, Listeria-immune spleen cells, 2 days after the infusion of 10,000 Listeria [4].
  • In contrast, PGF did not ameliorate splenomegaly, but caused increases in splenic asialo-GM1 (natural killer cells) and L3T4 (helper) positive T cells [5].

High impact information on Cd4

  • While an anti-clonotypic antibody completely inhibits this interaction, antibodies specific for another T cell receptor complex determinant, L3T4, only partially inhibit the cell-retrovirus interaction [2].
  • The other population expresses low levels of Thy-1, and lacks B220 as well as the T cell markers L3T4 and Lyt-2 [6].
  • Engagement of SLAM enhances antigen-specific proliferation and cytokine production by T cells carrying the CD4 antigen (CD4+) [7].
  • The virus enters cells by binding its envelope glycoprotein gp120 to the CD4 antigen present on brain and immune cells [8].
  • Thymocytes can be divided into four major compartments on the basis of surface expression of the murine equivalents of CD8 (Lyt-2) and CD4 (L3T4) (refs 1,2) [9].

Chemical compound and disease context of Cd4


Biological context of Cd4


Anatomical context of Cd4

  • Single-cell RT-PCR showed that the individual CD4+ lymphocyte expresses either the maternal or the paternal Cd4 allele, never both [16].
  • In addition, a small subpopulation comprising 2-3% of cells in the thymus and expressing neither Lyt-2 nor L3T4 has recently been described [17].
  • The minor species comigrates with the L3T4 transcript in T cells, whereas the major species is 1 kilobase smaller [12].
  • We show here for the first time that pluripotent hematopoietic stem cells express the CD4 antigen [18].
  • The phenotypically comparable Lyt-2- L3T4- subset of normal thymocytes expressed approximately 10-fold less TCR-alpha mRNA than normal lymph node cells and somewhat higher TCR-beta mRNA [19].

Associations of Cd4 with chemical compounds

  • The level of expression of the function-associated antigens CD4 (L3T4) and CD8 (Ly-2) decreased transiently early after activation with PMA/ionomycin, but not after stimulation with Con-A [20].
  • Surface marker analysis indicated that majority of effector cells which killed syngeneic lymphoblasts and activated M phi were Thy1+, asialo GM1+, L3T4-, Ly2- [21].
  • In vitro proliferation of murine spleen cells: inhibition by monoclonal antibodies to L3T4 and Lyt-2 T cell markers or intracellular cyclic adenosine monophosphate [22].
  • These populations of T cells could be distinguished in that they differed in their expression of the L3T4 and Lyt-2 cell surface molecules, in terms of their kinetic profiles of emergence and loss, and (c) in terms of their susceptibility to cyclophosphamide [23].
  • We have studied the effects of the steroid hormones, 17 beta-estradiol and dexamethasone, on the relative proportion of thymocyte expression of CD4 (L3T4), CD8 (Ly-2), TCR and IL-2R, used to identify different stages of thymocyte differentiation [24].

Physical interactions of Cd4

  • The expression of L3T4/Lyt-2 on murine T cells has led to the association of these surface markers with recognition of either class II or class I major histo-compatibility complex (MHC) antigens [25].
  • The currently accepted hypothesis used to explain the role of Lyt-2 and L3T4 in T cell activation proposes how these molecules interact with class I and II major histocompatibility complex molecules, respectively, on the antigen-presenting or target cell, to increase the avidity of binding of the antigen-specific T cell receptor [26].

Regulatory relationships of Cd4

  • The poor blocking observed with L3T4 mAb did not correlate with the almost complete blocking observed in the IL 2 response by the same hybridomas [27].

Other interactions of Cd4

  • Interferon-gamma-positive, L3T4-positive cells were detected in mirror sections [28].
  • With the use of a depletion method based on complement-mediated lysis with an anti-Lyt-2 monoclonal antibody (31 M) and a new anti-L3T4 monoclonal antibody (RL 172.4), we have purified the Lyt-2- L3T4- subset from lymph nodes or spleens of C57BL/6-lpr/lpr mice and determined whether they are immunologically functional in vitro [29].
  • In the case of stimulation with accessory cell-bound CD3 antibody, activation was blocked by LFA-1 but not L3T4 antibody [30].
  • The expression of L3T4 was also reduced in some of the experiments, while IL-2R was not expressed by the thymocytes, neither before nor after the co-culture [31].
  • Although Thy-1+ they were negative for other differentiation antigens including Ly-1, Ly-2 and L3T4 [32].

Analytical, diagnostic and therapeutic context of Cd4


  1. Role of L3T4 antigen in the activation of various functions of Lyt-1+2- T cells against vaccinia virus. Aoyama, A., Yoshioka, T., Sato, S., Mizushima, Y., Ogata, M., Ueda, S., Kato, S., Fujiwara, H., Hamaoka, T. Microbiol. Immunol. (1986) [Pubmed]
  2. A subset of T cell receptors associated with L3T4 molecules mediates C6VL leukemia cell binding of its cognate retrovirus. O'Neill, H.C., McGrath, M.S., Allison, J.P., Weissman, I.L. Cell (1987) [Pubmed]
  3. Alpha/beta T-cell antigen receptor gene and protein expression occurs at early stages of thymocyte differentiation. Richie, E.R., McEntire, B., Crispe, N., Kimura, J., Lanier, L.L., Allison, J.P. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  4. MHC-unrestricted transfer of antilisterial immunity by freshly isolated immune CD8 spleen cells. Lukacs, K., Kurlander, R.J. J. Immunol. (1989) [Pubmed]
  5. Modulation of murine schistosomiasis by exogenously administered prostaglandins. Chensue, S.W., Remick, D.G., Higashi, G.I., Boros, D.L., Kunkel, S.L. Am. J. Pathol. (1986) [Pubmed]
  6. Isolation of two early B lymphocyte progenitors from mouse marrow: a committed pre-pre-B cell and a clonogenic Thy-1-lo hematopoietic stem cell. Muller-Sieburg, C.E., Whitlock, C.A., Weissman, I.L. Cell (1986) [Pubmed]
  7. A novel receptor involved in T-cell activation. Cocks, B.G., Chang, C.C., Carballido, J.M., Yssel, H., de Vries, J.E., Aversa, G. Nature (1995) [Pubmed]
  8. Neuronal cell killing by the envelope protein of HIV and its prevention by vasoactive intestinal peptide. Brenneman, D.E., Westbrook, G.L., Fitzgerald, S.P., Ennist, D.L., Elkins, K.L., Ruff, M.R., Pert, C.B. Nature (1988) [Pubmed]
  9. Expression and function of the CD3-antigen receptor on murine CD4+8+ thymocytes. Havran, W.L., Poenie, M., Kimura, J., Tsien, R., Weiss, A., Allison, J.P. Nature (1987) [Pubmed]
  10. Immunotherapy for myasthenia gravis: a murine model. Christadoss, P., Dauphinee, M.J. J. Immunol. (1986) [Pubmed]
  11. Adoptive immunity to Mycobacterium bovis strain bacillus Calmette-Guérin by long-term cultured T-cell line in nude mice. Yamamoto, S., Iwai, H., Ueda, K. Immunology (1989) [Pubmed]
  12. Isolation and sequence of L3T4 complementary DNA clones: expression in T cells and brain. Tourvieille, B., Gorman, S.D., Field, E.H., Hunkapiller, T., Parnes, J.R. Science (1986) [Pubmed]
  13. Structure of the mouse gene encoding CD4 and an unusual transcript in brain. Gorman, S.D., Tourvieille, B., Parnes, J.R. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  14. The gene encoding the mouse T cell differentiation antigen L3T4 is located on chromosome 6. Field, E.H., Tourvieille, B., D'Eustachio, P., Parnes, J.R. J. Immunol. (1987) [Pubmed]
  15. Qualitative and quantitative studies of antigen-presenting cell function by using I-A-expressing L cells. Lechler, R.I., Norcross, M.A., Germain, R.N. J. Immunol. (1985) [Pubmed]
  16. Monoallelic expression of mouse Cd4 gene. Capparelli, R., Costabile, A., Viscardi, M., Iannelli, D. Mamm. Genome (2004) [Pubmed]
  17. Expression of interleukin-2 receptors as a differentiation marker on intrathymic stem cells. Ceredig, R., Lowenthal, J.W., Nabholz, M., MacDonald, H.R. Nature (1985) [Pubmed]
  18. CD4 is expressed on murine pluripotent hematopoietic stem cells. Wineman, J.P., Gilmore, G.L., Gritzmacher, C., Torbett, B.E., Müller-Sieburg, C.E. Blood (1992) [Pubmed]
  19. Abnormal expression of T cell receptor genes in Lyt-2- L3T4- lymphocytes of lpr mice: comparison with normal immature thymocytes. Miescher, G.C., Budd, R.C., Lees, R.K., MacDonald, H.R. J. Immunol. (1987) [Pubmed]
  20. The surface phenotype of activated T lymphocytes. Kelly, K., Shortman, K., Scollay, R. Immunol. Cell Biol. (1988) [Pubmed]
  21. Autocytotoxic activity of lymphokine-activated killer cells: characterization of effector cells and susceptible targets. Suzuki, H., Ikemoto, M., Kamitani, T., Hoshino, K., Yano, S. Anticancer Res. (1989) [Pubmed]
  22. In vitro proliferation of murine spleen cells: inhibition by monoclonal antibodies to L3T4 and Lyt-2 T cell markers or intracellular cyclic adenosine monophosphate. Zhou, P., Gorzynski, T., Dowjat, W.K., Rabin, R., Zaleski, M.B. Exp. Cell Biol. (1989) [Pubmed]
  23. The kinetics of emergence and loss of mediator T lymphocytes acquired in response to infection with Mycobacterium tuberculosis. Orme, I.M. J. Immunol. (1987) [Pubmed]
  24. Steroid sensitivity of thymocyte subpopulations during intrathymic differentiation. Effects of 17 beta-estradiol and dexamethasone on subsets expressing T cell antigen receptor or IL-2 receptor. Screpanti, I., Morrone, S., Meco, D., Santoni, A., Gulino, A., Paolini, R., Crisanti, A., Mathieson, B.J., Frati, L. J. Immunol. (1989) [Pubmed]
  25. Role of L3T4 in antigen-driven activation of a class I-specific T cell hybridoma. Greenstein, J.L., Malissen, B., Burakoff, S.J. J. Exp. Med. (1985) [Pubmed]
  26. Accessory molecules in T lymphocyte activation. Miller, J.F. Int. Arch. Allergy Appl. Immunol. (1987) [Pubmed]
  27. Specific lymphocyte-target cell conjugate formation between tumor-specific helper T-cell hybridomas and IA-bearing RCS tumors and IE-bearing allogeneic cells. I. Role of Ia and both L3T4 and LFA-1 antigens in recognition/binding. Ohnishi, K., Bonavida, B. J. Immunol. (1986) [Pubmed]
  28. Administration of interleukin-2 induces major histocompatibility complex class II expression on the biliary epithelial cells, possibly through endogenous interferon-gamma production. Himeno, H., Saibara, T., Onishi, S., Yamamoto, Y., Enzan, H. Hepatology (1992) [Pubmed]
  29. Functional analysis of T cell subsets from mice bearing the lpr gene. Davignon, J.L., Budd, R.C., Ceredig, R., Piguet, P.F., MacDonald, H.R., Cerottini, J.C., Vassalli, P., Izui, S. J. Immunol. (1985) [Pubmed]
  30. The role of accessory molecules in T-helper activation induced by antigen, lectin, or CD3 antibodies. Torbett, B.E., Clark, W.R. Cell. Immunol. (1988) [Pubmed]
  31. Contact-mediated maturational effects of thymic stromal cells on murine thymocytes in culture. Savion, S., Itoh, T., Hertogs, H., Shoham, J. Immunology (1989) [Pubmed]
  32. In vitro properties of murine autoreactive T cell clones with specificity for erythrocytes. Gibson, J., Basten, A. Autoimmunity (1988) [Pubmed]
  33. Proliferation in vitro and interleukin production by 14 day fetal and adult Lyt-2-/L3T4- mouse thymocytes. Ceredig, R. J. Immunol. (1986) [Pubmed]
  34. Morphological analysis of selective destruction of pancreatic beta-cells by cytotoxic T lymphocytes in NOD mice. Hayakawa, M., Yokono, K., Nagata, M., Hatamori, N., Ogawa, W., Miki, A., Mizoguti, H., Baba, S. Diabetes (1991) [Pubmed]
  35. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. Dialynas, D.P., Quan, Z.S., Wall, K.A., Pierres, A., Quintáns, J., Loken, M.R., Pierres, M., Fitch, F.W. J. Immunol. (1983) [Pubmed]
WikiGenes - Universities