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Gene Review

Ncan  -  neurocan

Mus musculus

Synonyms: C230035B04, Chondroitin sulfate proteoglycan 3, Cspg3, Cspg3-rs, Neurocan core protein, ...
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Disease relevance of Ncan

  • In the developing chick, neural retina neurocan is present in the inner plexiform layer from day 7 on, and the GalNAcPTase receptor becomes restricted to the inner nuclear layer and the ganglion cell layer (as well as the fiber layer), the two forming a sandwich [1].

High impact information on Ncan


Biological context of Ncan

  • The present results support our previous hypothesis proposed for normal rats that a heterophilic molecular interaction between L1 and neurocan is involved in determining the thalamocortical pathway within the neocortical anlage [T. Fukuda et al. (1997) Journal of Comparative Neurology, 382, 141-152] [6].
  • Mapping of a defined neurocan binding site to distinct domains of tenascin-C [7].
  • Cosmid clones containing the mouse neurocan gene were isolated from a genomic library using rat neurocan cDNA fragments as probe [8].
  • Single-strand conformation polymorphism was used to map neurocan to chromosome 8 between the microsatellite markers D8Mit29 and D8Mit78 [8].
  • A centrifugation cell adhesion assay indicated that both the N- and C-terminal neurocan fragments could interact with these HSPGs expressed on the cell surface [9].

Anatomical context of Ncan


Associations of Ncan with chemical compounds

  • During normal development of the mouse neocortex, thalamic axons immunoreactive for the neural cell adhesion molecule L1 rarely invaded the cortical plate and ran centered in the subplate which is immunoreactive for neurocan, a brain-specific chondroitin sulfate proteoglycan [6].
  • Neurocan immunoreactivity was associated with the bromodeoxyuridine-positive cells in the superplate, as well as being present in oblique bands within the cortical plate, along which L1-bearing thalamic axons preferentially ran [6].
  • A new monoclonal antibody (designated 1F6), which recognizes an epitope in the N-terminal portion of neurocan, has been used for the isolation of proteolytic processing fragments that occur together with link protein in a complex with hyaluronic acid [12].
  • Neurocan is a heparin binding proteoglycan [11].

Physical interactions of Ncan

  • The wild type neurocan hyaluronan binding domain fused to alkaline phosphatase bound to immobilized hyaluronan under physiological as well as moderately hypertonic conditions, whereas its ability to bind to immobilized chondroitin sulfate dropped rapidly with increasing salt concentration [13].

Regulatory relationships of Ncan


Other interactions of Ncan

  • Further, an almost quadruple knockout like extracellular environment was noticed in the brains of triple knockout mice lacking both tenascins and brevican, since these brains had strongly reduced levels of neurocan [15].
  • Immunoreactivity for neurocan was first detected in primary olfactory neurons at embryonic day 11 [10].
  • This alteration reflects the loss of phosphacan and neurocan from cortical nets in mice deficient in tenascin-R [16].
  • The N-terminal fragments of neurocan are also recognized by monoclonal antibodies (5C4, 8A4, and 3B1) to epitopes in the G1 and G2 domains of aggrecan and/or in the hyaluronic acid-binding domain of link protein [12].
  • Moreover, this preincubation mediated also the binding of a fusion protein representing only the immunoglobulin module of neurocan linked to alkaline phosphatase, which showed no binding to immobilized hyaluronan alone [13].

Analytical, diagnostic and therapeutic context of Ncan


  1. Coordinate regulation of cadherin and integrin function by the chondroitin sulfate proteoglycan neurocan. Li, H., Leung, T.C., Hoffman, S., Balsamo, J., Lilien, J. J. Cell Biol. (2000) [Pubmed]
  2. Neurocan is dispensable for brain development. Zhou, X.H., Brakebusch, C., Matthies, H., Oohashi, T., Hirsch, E., Moser, M., Krug, M., Seidenbecher, C.I., Boeckers, T.M., Rauch, U., Buettner, R., Gundelfinger, E.D., Fässler, R. Mol. Cell. Biol. (2001) [Pubmed]
  3. Characterization of the L1-neurocan-binding site. Implications for L1-L1 homophilic binding. Oleszewski, M., Gutwein, P., von der Lieth, W., Rauch, U., Altevogt, P. J. Biol. Chem. (2000) [Pubmed]
  4. Role of Src kinases in the ADAM-mediated release of L1 adhesion molecule from human tumor cells. Gutwein, P., Oleszewski, M., Mechtersheimer, S., Agmon-Levin, N., Krauss, K., Altevogt, P. J. Biol. Chem. (2000) [Pubmed]
  5. Isolation of a tenascin-R binding protein from mouse brain membranes. A phosphacan-related chondroitin sulfate proteoglycan. Xiao, Z.C., Bartsch, U., Margolis, R.K., Rougon, G., Montag, D., Schachner, M. J. Biol. Chem. (1997) [Pubmed]
  6. Aberrant trajectory of thalamocortical axons associated with abnormal localization of neurocan immunoreactivity in the cerebral neocortex of reeler mutant mice. Li, H.P., Oohira, A., Ogawa, M., Kawamura, K., Kawano, H. Eur. J. Neurosci. (2005) [Pubmed]
  7. Mapping of a defined neurocan binding site to distinct domains of tenascin-C. Rauch, U., Clement, A., Retzler, C., Fröhlich, L., Fässler, R., Göhring, W., Faissner, A. J. Biol. Chem. (1997) [Pubmed]
  8. Structure and chromosomal localization of the mouse neurocan gene. Rauch, U., Grimpe, B., Kulbe, G., Arnold-Ammer, I., Beier, D.R., Fässler, R. Genomics (1995) [Pubmed]
  9. Heparan sulphate proteoglycans interact with neurocan and promote neurite outgrowth from cerebellar granule cells. Akita, K., Toda, M., Hosoki, Y., Inoue, M., Fushiki, S., Oohira, A., Okayama, M., Yamashina, I., Nakada, H. Biochem. J. (2004) [Pubmed]
  10. Dynamic spatiotemporal expression patterns of neurocan and phosphacan indicate diverse roles in the developing and adult mouse olfactory system. Clarris, H.J., Rauch, U., Key, B. J. Comp. Neurol. (2000) [Pubmed]
  11. Neurocan is a heparin binding proteoglycan. Feng, K., Arnold-Ammer, I., Rauch, U. Biochem. Biophys. Res. Commun. (2000) [Pubmed]
  12. Chondroitin sulfate and chondroitin/keratan sulfate proteoglycans of nervous tissue: developmental changes of neurocan and phosphacan. Meyer-Puttlitz, B., Milev, P., Junker, E., Zimmer, I., Margolis, R.U., Margolis, R.K. J. Neurochem. (1995) [Pubmed]
  13. Cartilage link protein interacts with neurocan, which shows hyaluronan binding characteristics different from CD44 and TSG-6. Rauch, U., Hirakawa, S., Oohashi, T., Kappler, J., Roos, G. Matrix Biol. (2004) [Pubmed]
  14. Expression of phosphacan and neurocan during early development of mouse retinofugal pathway. Leung, K.M., Margolis, R.U., Chan, S.O. Brain Res. Dev. Brain Res. (2004) [Pubmed]
  15. Extracellular matrix alterations in brains lacking four of its components. Rauch, U., Zhou, X.H., Roos, G. Biochem. Biophys. Res. Commun. (2005) [Pubmed]
  16. Morphology of perineuronal nets in tenascin-R and parvalbumin single and double knockout mice. Haunsø, A., Ibrahim, M., Bartsch, U., Letiembre, M., Celio, M.R., Menoud, P. Brain Res. (2000) [Pubmed]
  17. The mRNA expression of neurocan, a brain-specific chondroitin sulfate proteoglycan, in neoplastic mammary glands in mice. Harigaya, T., Ogawa, H., Tsunoda, S., Nagasawa, H. Zool. Sci. (1996) [Pubmed]
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