The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

Dnmt3a  -  DNA methyltransferase 3A

Mus musculus

Synonyms: AU015806, DNA (cytosine-5)-methyltransferase 3A, DNA MTase MmuIIIA, DNA methyltransferase MmuIIIA, M.MmuIIIA, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Dnmt3a


High impact information on Dnmt3a

  • In this system, Dnmt3a functioned as a de novo methyltransferase, whereas Dnmt1 had no detectable de novo methylation activity [6].
  • When co-expressed, Dnmt1 and Dnmt3a cooperated to establish and maintain methylation patterns [6].
  • Inactivation of both Dnmt3a and Dnmt3b, DNA methyltransferases essential for the initiation of de novo DNA methylation, abolished the establishment of DNA methylation and the silencing of Rhox cluster genes in the embryo proper [7].
  • These findings suggest that rapid methylation of the evolutionarily conserved -53 CpG by Dnmt3a may suppress IFN-gamma transcription in developing TH2 cells by directly inhibiting transcription factor binding [8].
  • The PWWP domain is a weakly conserved sequence motif found in > 60 eukaryotic proteins, including the mammalian DNA methyltransferases Dnmt3a and Dnmt3b [9].

Biological context of Dnmt3a


Anatomical context of Dnmt3a


Associations of Dnmt3a with chemical compounds


Co-localisations of Dnmt3a


Regulatory relationships of Dnmt3a

  • Dnmt3b is specifically expressed in the totipotent cells of mouse early embryos and Dnmt3a, a longer form of the two isoforms, is ubiquitously expressed in mesenchyme cells after the 10 day embryo stage [Mech. Dev. 118 (2002) 187] [20].

Other interactions of Dnmt3a


Analytical, diagnostic and therapeutic context of Dnmt3a


  1. Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b. Chen, T., Ueda, Y., Dodge, J.E., Wang, Z., Li, E. Mol. Cell. Biol. (2003) [Pubmed]
  2. Biochemical fractionation reveals association of DNA methyltransferase (Dnmt) 3b with Dnmt1 and that of Dnmt 3a with a histone H3 methyltransferase and Hdac1. Datta, J., Ghoshal, K., Sharma, S.M., Tajima, S., Jacob, S.T. J. Cell. Biochem. (2003) [Pubmed]
  3. Preferential methylation of unmethylated DNA by Mammalian de novo DNA methyltransferase Dnmt3a. Yokochi, T., Robertson, K.D. J. Biol. Chem. (2002) [Pubmed]
  4. Enzymatic properties of recombinant Dnmt3a DNA methyltransferase from mouse: the enzyme modifies DNA in a non-processive manner and also methylates non-CpG [correction of non-CpA] sites. Gowher, H., Jeltsch, A. J. Mol. Biol. (2001) [Pubmed]
  5. De novo methylation of MMLV provirus in embryonic stem cells: CpG versus non-CpG methylation. Dodge, J.E., Ramsahoye, B.H., Wo, Z.G., Okano, M., Li, E. Gene (2002) [Pubmed]
  6. Mammalian (cytosine-5) methyltransferases cause genomic DNA methylation and lethality in Drosophila. Lyko, F., Ramsahoye, B.H., Kashevsky, H., Tudor, M., Mastrangelo, M.A., Orr-Weaver, T.L., Jaenisch, R. Nat. Genet. (1999) [Pubmed]
  7. DNA methylation regulates long-range gene silencing of an X-linked homeobox gene cluster in a lineage-specific manner. Oda, M., Yamagiwa, A., Yamamoto, S., Nakayama, T., Tsumura, A., Sasaki, H., Nakao, K., Li, E., Okano, M. Genes Dev. (2006) [Pubmed]
  8. Inhibition of IFN-gamma transcription by site-specific methylation during T helper cell development. Jones, B., Chen, J. EMBO J. (2006) [Pubmed]
  9. The PWWP domain of mammalian DNA methyltransferase Dnmt3b defines a new family of DNA-binding folds. Qiu, C., Sawada, K., Zhang, X., Cheng, X. Nat. Struct. Biol. (2002) [Pubmed]
  10. DNA methylation density influences the stability of an epigenetic imprint and Dnmt3a/b-independent de novo methylation. Lorincz, M.C., Schübeler, D., Hutchinson, S.R., Dickerson, D.R., Groudine, M. Mol. Cell. Biol. (2002) [Pubmed]
  11. Preference of DNA methyltransferases for CpG islands in mouse embryonic stem cells. Hattori, N., Abe, T., Hattori, N., Suzuki, M., Matsuyama, T., Yoshida, S., Li, E., Shiota, K. Genome Res. (2004) [Pubmed]
  12. Molecular enzymology of the catalytic domains of the Dnmt3a and Dnmt3b DNA methyltransferases. Gowher, H., Jeltsch, A. J. Biol. Chem. (2002) [Pubmed]
  13. DNA methyltransferase expression in the mouse germ line during periods of de novo methylation. Lees-Murdock, D.J., Shovlin, T.C., Gardiner, T., De Felici, M., Walsh, C.P. Dev. Dyn. (2005) [Pubmed]
  14. Inactivation of Dnmt3b in mouse embryonic fibroblasts results in DNA hypomethylation, chromosomal instability, and spontaneous immortalization. Dodge, J.E., Okano, M., Dick, F., Tsujimoto, N., Chen, T., Wang, S., Ueda, Y., Dyson, N., Li, E. J. Biol. Chem. (2005) [Pubmed]
  15. Dynamic expression of DNMT3a and DNMT3b isoforms during male germ cell development in the mouse. La Salle, S., Trasler, J.M. Dev. Biol. (2006) [Pubmed]
  16. De novo DNA methyltransferases Dnmt3a and Dnmt3b primarily mediate the cytotoxic effect of 5-aza-2'-deoxycytidine. Oka, M., Meacham, A.M., Hamazaki, T., Rodić, N., Chang, L.J., Terada, N. Oncogene (2005) [Pubmed]
  17. Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b. Tsumura, A., Hayakawa, T., Kumaki, Y., Takebayashi, S., Sakaue, M., Matsuoka, C., Shimotohno, K., Ishikawa, F., Li, E., Ueda, H.R., Nakayama, J., Okano, M. Genes Cells (2006) [Pubmed]
  18. Catalytic mechanism of DNA-(cytosine-C5)-methyltransferases revisited: covalent intermediate formation is not essential for methyl group transfer by the murine Dnmt3a enzyme. Reither, S., Li, F., Gowher, H., Jeltsch, A. J. Mol. Biol. (2003) [Pubmed]
  19. Dnmt3a and Dnmt3b are transcriptional repressors that exhibit unique localization properties to heterochromatin. Bachman, K.E., Rountree, M.R., Baylin, S.B. J. Biol. Chem. (2001) [Pubmed]
  20. Expression of Dnmt3b in mouse hematopoietic progenitor cells and spermatogonia at specific stages. Watanabe, D., Suetake, I., Tajima, S., Hanaoka, K. Gene Expr. Patterns (2004) [Pubmed]
  21. Differential expression of DNA-methyltransferases in drug resistant murine neuroblastoma cells. Qiu, Y.Y., Mirkin, B.L., Dwivedi, R.S. Cancer Detect. Prev. (2002) [Pubmed]
  22. Deregulation of DNA methyltransferases and loss of parental methylation at the insulin-like growth factor II (Igf2)/H19 loci in p53 knockout mice prior to tumor development. Park, I.Y., Sohn, B.H., Choo, J.H., Joe, C.O., Seong, J.K., Lee, Y.I., Chung, J.H. J. Cell. Biochem. (2005) [Pubmed]
  23. Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system. Feng, J., Chang, H., Li, E., Fan, G. J. Neurosci. Res. (2005) [Pubmed]
  24. Neonatal exposure to diethylstilbestrol alters the expression of DNA methyltransferases and methylation of genomic DNA in the epididymis of mice. Sato, K., Fukata, H., Kogo, Y., Ohgane, J., Shiota, K., Mori, C. Endocr. J. (2006) [Pubmed]
  25. Assignment of cytosine-5 DNA methyltransferases Dnmt3a and Dnmt3b to mouse chromosome bands 12A2-A3 and 2H1 by in situ hybridization. Okano, M., Takebayashi, S., Okumura, K., Li, E. Cytogenet. Cell Genet. (1999) [Pubmed]
WikiGenes - Universities