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Gene Review

Mpst  -  mercaptopyruvate sulfurtransferase

Rattus norvegicus

Synonyms: 3-mercaptopyruvate sulfurtransferase, MST
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Disease relevance of Mpst

  • Cells of a brown Norway (BN) rat Moloney sarcoma (MST) failed to express certain BN alloantigen specificities and bound only about 30-50% the amount of labeled alloantibody bound by normal BN spleen cells [1].
  • E. coli C32S Trx, however, did not activate MST [2].
  • BN rats immunized subcutaneously with a viral induced tumor (MST) or with a chemical-induced fibrosarcoma (BC5) were donors of immune spleen cells [3].
  • We have cloned, by cross-hybridization with the cDNA from rat liver heparan sulfate N-deacetylase/N-sulfotransferase, a protein from a heparin synthesizing mastocytoma derived cell line called MST [4].

High impact information on Mpst

  • The identification of the FOXO transcription factors as major and evolutionarily conserved targets of MST1 suggests that MST kinases play important roles in diverse biological processes including cellular responses to oxidative stress and longevity [5].
  • Further loss of alloantigens that occurred with prolonged in vitro culture was associated with reduced virulence of MST cells for syngeneic hosts and with increased expression of tumor-associated antigens [1].
  • On the other hand, reduced glutathione did not affect MST activity [2].
  • Reduced Trx turns on a redox switch for the enzymatic activation of MST, which contributes to the maintenance of cellular redox homeostasis [2].
  • Rat 3-mercaptopyruvate sulfurtransferase (MST) contains three exposed cysteines as follows: a catalytic site cysteine, Cys(247), in the active site and Cys(154) and Cys(263) on the surface of MST [2].

Chemical compound and disease context of Mpst

  • E. coli C35S Trx, in which Cys(35) was replaced with Ser, formed some adducts with MST and activated MST after treatment with DTT [2].
  • Rat Moloney sarcoma cells (MST) were pulsed with 35S-L-methionine for 10 and 60 min and lysed by vortexing in 0.5% deoxycholate, 0.5% NP40, 0.02 M Tris, 0.05 M NaCl, pH 7.5, for 30 sec [6].
  • The dissociated cell surface membranes of a rat Moloney sarcoma (MST), derived from a BN rat, were extracted with 2 M KI, with 6 M guanidine thiocyanate, or by papain digestion [7].

Biological context of Mpst


Anatomical context of Mpst

  • MST was predominantly localized in proximal tubular epithelium in the kidney, pericentral hepatocytes in the liver, cardiac cells in the heart, and neuroglial cells in the brain [11].
  • This immunocytochemical study also found that MST was localized in both mitochondria and cytoplasm [11].
  • Also, the growth of BC5 was not affected by MST immune spleen cells cultured for 7 days with MST and/or BC5 [3].
  • Complexes were localized in the spleen of tumor-bearing and control rats, but much more in spleens of MST-bearing rats [12].
  • These results suggest that the MST-derived enzyme is probably unique to the production of heparin in mast cells [4].

Associations of Mpst with chemical compounds


Regulatory relationships of Mpst


Other interactions of Mpst


Analytical, diagnostic and therapeutic context of Mpst

  • A complete amino acid structure of rat liver mercaptopyruvate sulfurtransferase (MST, EC was determined by sequence analysis of cDNA and purified enzyme [9].
  • LEW X BN)F1 cardiac allografts survive 1 wk in unmodified LEW recipients (MST +/- SD = 8.3 +/- 1.2 days) but indefinitely (greater than 100 days) in B rats, produced by 750 R sublethal x-radiation 3 to 4 wk after adult thymectomy and reconstitution with syngeneic bone marrow cells from thymectomized thoracic duct-drained donors [14].
  • The unresponsive state is eventually reversed by adoptive transfer of 10(8) spleen cells from nonimmune (MST +/- SD = 27.5 +/- 4.7 days) or alloimmune (MST +/- SD = 21.5 +/- 1.9 days) syngeneic animals [14].
  • Wistar-Furth islets, which were UV-irradiated at 850-900 J/m2 and cultured for 24 hr prior to transplantation, did not survive any longer than those in control animals receiving untreated islets (MST 5.5 +/- 1 day) [15].
  • The MST for the control group was 9.89 +/- 0.35 days [16].


  1. Alloantigen expression of a rat Moloney sarcoma. Jones, J.M., Feldman, J.D. J. Natl. Cancer Inst. (1975) [Pubmed]
  2. Thioredoxin-dependent enzymatic activation of mercaptopyruvate sulfurtransferase. An intersubunit disulfide bond serves as a redox switch for activation. Nagahara, N., Yoshii, T., Abe, Y., Matsumura, T. J. Biol. Chem. (2007) [Pubmed]
  3. In vivo elimination by specific effector cells of an established syngeneic rat moloney virus-induced sarcoma. Fernandez-Cruz, E., Halliburton, B., Feldman, J.D. J. Immunol. (1979) [Pubmed]
  4. Molecular cloning and expression of a glycosaminoglycan N-acetylglucosaminyl N-deacetylase/N-sulfotransferase from a heparin-producing cell line. Orellana, A., Hirschberg, C.B., Wei, Z., Swiedler, S.J., Ishihara, M. J. Biol. Chem. (1994) [Pubmed]
  5. A conserved MST-FOXO signaling pathway mediates oxidative-stress responses and extends life span. Lehtinen, M.K., Yuan, Z., Boag, P.R., Yang, Y., Villén, J., Becker, E.B., DiBacco, S., de la Iglesia, N., Gygi, S., Blackwell, T.K., Bonni, A. Cell (2006) [Pubmed]
  6. Tumor-associated antigens of rat moloney sarcoma cells. II. Cytosol antigens. Leung, K., Feldman, J.D. J. Immunol. (1978) [Pubmed]
  7. Tumor-associated antigens of rat moloney sarcoma cells. I. Cell-surface antigens. Leung, K., Jones, J.M., Feldman, J.D. J. Immunol. (1978) [Pubmed]
  8. Cytosolic mercaptopyruvate sulfurtransferase is evolutionarily related to mitochondrial rhodanese. Striking similarity in active site amino acid sequence and the increase in the mercaptopyruvate sulfurtransferase activity of rhodanese by site-directed mutagenesis. Nagahara, N., Okazaki, T., Nishino, T. J. Biol. Chem. (1995) [Pubmed]
  9. Role of amino acid residues in the active site of rat liver mercaptopyruvate sulfurtransferase. CDNA cloning, overexpression, and site-directed mutagenesis. Nagahara, N., Nishino, T. J. Biol. Chem. (1996) [Pubmed]
  10. Post-translational regulation of mercaptopyruvate sulfurtransferase via a low redox potential cysteine-sulfenate in the maintenance of redox homeostasis. Nagahara, N., Katayama, A. J. Biol. Chem. (2005) [Pubmed]
  11. Tissue and subcellular distribution of mercaptopyruvate sulfurtransferase in the rat: confocal laser fluorescence and immunoelectron microscopic studies combined with biochemical analysis. Nagahara, N., Ito, T., Kitamura, H., Nishino, T. Histochem. Cell Biol. (1998) [Pubmed]
  12. p30-Anti-p30 immune complexes: intravascular clearance and extravascular sequestration in rats bearing Moloney sarcoma. Jennette, J.C., Feldman, J.D. J. Immunol. (1978) [Pubmed]
  13. L-cysteine metabolism in guinea pig and rat tissues. Wróbel, M., Ubuka, T., Yao, W.B., Abe, T. Comp. Biochem. Physiol. B, Biochem. Mol. Biol. (1997) [Pubmed]
  14. Restoration of allograft responsiveness in B rats by interleukin 2 and/or adherent cells. Clason, A.E., Duarte, A.J., Kupiec-Weglinski, J.W., Williams, J.N., Wang, B.S., Strom, T.B., Tilney, N.L. J. Immunol. (1982) [Pubmed]
  15. The use of direct ultraviolet irradiation and cyclosporine in facilitating indefinite pancreatic islet allograft acceptance. Lau, H., Reemtsma, K., Hardy, M.A. Transplantation (1984) [Pubmed]
  16. The synergistic effect of low-dose cyclosporine and fluocinolone acetonide on the survival of rat allogeneic skin grafts. Zhao, X.F., Alexander, J.W., Schroeder, T., Babcock, G.F. Transplantation (1988) [Pubmed]
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