The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

Ugt2b37  -  UDP-glucuronosyltransferase 2 family,...

Rattus norvegicus

Synonyms: 17-beta-hydroxysteroid-specific UDPGT, UDP-glucuronosyltransferase 2B37, UDP-glucuronosyltransferase R-21, UDPGT 2B37, UDPGTr-21, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Ugt2b5

  • Gunn rats are a mutant strain of Wistar rats that have unconjugated hyperbilirubinemia due to absence of hepatic uridine diphosphate-glucuronosyltransferase (UDPGT; EC. activity toward bilirubin [1].
  • The inbred homozygous Gunn rat is also deficient in UDPGT, exhibits unconjugated hyperbilirubinemia, and is an excellent animal model of the Crigler-Najjar syndrome [2].
  • 4-CB might also alter the normal imprinting of sexual differentiation of hepatic metabolism as evidenced by a feminization (decrease) of UDPGT in adult male rats which were exposed neonatally to 4-CB [3].
  • These results indicate that the effect of SF is similar to those of PB and thyroid hypertrophy seen in the oral 2-week treatment with SF, and may be caused by indirectly elevated TSH levels which resulted from the induction of hepatic UDPGT activity [4].

High impact information on Ugt2b5

  • UDPGT isoform I (elution pH 8.7) from Wistar (RHA) and Gunn rats was active toward 4-nitrophenol [1].
  • Isoforms II (elution pH 8.4), III (elution pH 8.0), and IV (elution pH 7.8) from Gunn rats had normal UDPGT activities, except that Isoform IV was inactive toward bilirubin [1].
  • The corresponding isoform from Gunn rat liver was enzymically inactive but exhibited normal elution pH and mobility on NaDodSO4/polyacrylamide gel electrophoresis (Mr 53,000), and was recognized by a UDPGT-specific antiserum [1].
  • The isoform from Gunn rat liver had only 10% of normal UDPGT activity, however UDPGT activity increased to normal upon addition of 15 mM diethylnitrosamine in vitro [1].
  • Of the purified enzymes investigated, only 3 alpha-hydroxysteroid UDPGT catalyzed the glucuronidation of 4-aminobiphenyl. alpha-Naphthylamine and beta-naphthylamine conjugations were catalyzed by 3 alpha-hydroxysteroid, 17 beta-hydroxysteroid, and 3-methylcholanthrene-inducible p-nitrophenol UDPGTs [5].

Biological context of Ugt2b5


Anatomical context of Ugt2b5

  • Expression of UDPGTr-5 cDNA in COS cells demonstrated that the encoded enzyme has a subunit size of 50 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis [6].
  • Immunoblot analysis of microsomes from developing rat liver demonstrated that the deficiency in bilirubin and testosterone glucuronidation in the fetus was due to the absence of the UDPGT isoenzyme proteins responsible for these conjugations [11].
  • Using an improved procedure to isolate pure bile ductular epithelial cells from rat liver, we were able to define UDP-glucuronosyl transferase (UDPGT) isozymes expressed in these cells under physiological conditions [12].
  • CONCLUSION: Transplants of small intestine with known UDPGT activity partially corrected the deficiency in Gunn rats and allayed the hyperbilirubinemia [2].
  • Steroid glucuronidation was investigated in solubilized female rat and rabbit liver microsomes and in preparations of UDP-glucuronsyltransferase (UDPGT) activity resolved from these organelles [13].

Associations of Ugt2b5 with chemical compounds


Other interactions of Ugt2b5

  • Although Crabp2 had been identified as one of the 16 genes differentially expressed between ACI and BUF rats with cDNA-RDA, Fabp1 and Ugt2b5 were newly identified in this study [14].

Analytical, diagnostic and therapeutic context of Ugt2b5


  1. Isolation of multiple normal and functionally defective forms of uridine diphosphate-glucuronosyltransferase from inbred Gunn rats. Roy Chowdhury, N., Gross, F., Moscioni, A.D., Kram, M., Arias, I.M., Roy Chowdhury, J. J. Clin. Invest. (1987) [Pubmed]
  2. Correction of congenital indirect hyperbilirubinemia by small intestinal transplantation. Medley, M.M., Hooker, R.L., Rabinowitz, S., Holton, R., Jaffe, B.M. Am. J. Surg. (1995) [Pubmed]
  3. Developmental toxicology of the halogenated aromatics: effects on enzyme development. Lucier, G.W., McDaniel, O.S. Ann. N. Y. Acad. Sci. (1979) [Pubmed]
  4. Thyroid hypertrophic effect of semotiadil fumarate, a new calcium antagonist, in rats. Tsuchiya, Y., Ohno, H., Satoh, H., Sugawara, T., Kajimura, T., Nomura, M. The Journal of toxicological sciences. (2000) [Pubmed]
  5. N-glucuronidation of carcinogenic aromatic amines catalyzed by rat hepatic microsomal preparations and purified rat liver uridine 5'-diphosphate-glucuronosyltransferases. Green, M.D., Tephly, T.R. Cancer Res. (1987) [Pubmed]
  6. The cDNA sequence and expression of a variant 17 beta-hydroxysteroid UDP-glucuronosyltransferase. Mackenzie, P.I. J. Biol. Chem. (1990) [Pubmed]
  7. Photoaffinity labeling of rat liver microsomal morphine UDP-glucuronosyltransferase by [3H]flunitrazepam. Thomassin, J., Tephly, T.R. Mol. Pharmacol. (1990) [Pubmed]
  8. Thyroxine and 3,3',5-triiodothyronine are glucuronidated in rat liver by different uridine diphosphate-glucuronyltransferases. Beetstra, J.B., van Engelen, J.G., Karels, P., van der Hoek, H.J., de Jong, M., Docter, R., Krenning, E.P., Hennemann, G., Brouwer, A., Visser, T.J. Endocrinology (1991) [Pubmed]
  9. Immunochemical and functional characterization of UDP-glucuronosyltransferases from rat liver, intestine and kidney. Koster, A.S., Schirmer, G., Bock, K.W. Biochem. Pharmacol. (1986) [Pubmed]
  10. Glucuronidation of 3'-azido-3'-deoxythymidine: human and rat enzyme specificity. Resetar, A., Spector, T. Biochem. Pharmacol. (1989) [Pubmed]
  11. The inadequacy of perinatal glucuronidation: immunoblot analysis of the developmental expression of individual UDP-glucuronosyltransferase isoenzymes in rat and human liver microsomes. Coughtrie, M.W., Burchell, B., Leakey, J.E., Hume, R. Mol. Pharmacol. (1988) [Pubmed]
  12. 17 Beta-hydroxysteroid UDP-glucuronosyl transferase is expressed in bile ductular epithelial cells under physiological conditions. Franson, K.L., Burchell, B., Mathis, G.A. Biochem. Biophys. Res. Commun. (1990) [Pubmed]
  13. Steroid 3- and 17-OH UDP-glucuronosyltransferase activities in rat and rabbit liver microsomes. Falany, C.N., Chowdhury, J.R., Chowdhury, N.R., Tephly, T.R. Drug Metab. Dispos. (1983) [Pubmed]
  14. Differential expression of genes related to levels of mucosal cell proliferation among multiple rat strains by using oligonucleotide microarrays. Yamashita, S., Nomoto, T., Ohta, T., Ohki, M., Sugimura, T., Ushijima, T. Mamm. Genome (2003) [Pubmed]
  15. Chemoprotective effects of garden cress (Lepidium sativum) and its constituents towards 2-amino-3-methyl-imidazo[4,5-f]quinoline (IQ)-induced genotoxic effects and colonic preneoplastic lesions. Kassie, F., Rabot, S., Uhl, M., Huber, W., Qin, H.M., Helma, C., Schulte-Hermann, R., Knasmüller, S. Carcinogenesis (2002) [Pubmed]
  16. Identification and biochemical analysis of novel olfactory-specific cytochrome P-450IIA and UDP-glucuronosyl transferase. Lazard, D., Tal, N., Rubinstein, M., Khen, M., Lancet, D., Zupko, K. Biochemistry (1990) [Pubmed]
  17. Purification and properties of rat kidney UDP-glucuronosyltransferase. Coughtrie, M.W., Burchell, B., Bend, J.R. Biochem. Pharmacol. (1987) [Pubmed]
  18. Separation, purification and characterization of three isoenzymes of UDP-glucuronyltransferase from rat liver microsomes. Falany, C.N., Tephly, T.R. Arch. Biochem. Biophys. (1983) [Pubmed]
  19. Alterations in hepatic detoxifying enzymes induced by new organophosphorus insecticides following subchronic exposure in rats. Mahboob, M., Siddiqui, M.K. Journal of applied toxicology : JAT. (2001) [Pubmed]
WikiGenes - Universities