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Gene Review

lipA  -  triacylglycerol lipase

Pseudomonas protegens Pf-5

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Disease relevance of PFL_0617

  • Serratia marcescens ATP-binding cassette (ABC) exporter, the Lip system, secretes lipase (LipA(SM)), metalloproteases, and a cell surface layer protein homologue but not a heme acquisition protein, HasA (HasA(SM)) [1].
  • The Pseudomonas fluorescens lipase has a C-terminal secretion signal and is secreted by a three-component bacterial ABC-exporter system [2].
  • In Escherichia coli, the three proteins AprD, AprE, AprF are necessary and sufficient for efficient secretion of lipase to the extracellular medium [2].
  • Formation of orange-red fluorescent plaques on rhodamine B-triolein plates was used to identify phages carrying the lipase gene [3].
  • A threading model of PhaC1Po, developed based on homology of the enzyme to the Burkholderia glumae lipase, suggested that the amino acid substitutions found in the active chimeras were located mostly on the protein model surface [4].

High impact information on PFL_0617

  • The neutral lipase from the bacteria Pseudomonas fluorescens, marketed under the trade name LpL-200S, has been crystallized in a form suitable for X-ray diffraction analysis from 35% n-propanol at pH 8 [5].
  • We conclude that any lipase causing lipolysis of chylomicrons can stimulate their clearance by the liver, but that lipoprotein lipase has an additional effect on the removal, which is not dependent on its catalytic activity [6].
  • Four selected mutants from one round of error-prone PCR mutagenesis, T6, T8, T24, and T35, showed 3.2-, 2.6-, 2.9-, and 3.0-fold increases in the level of secretion of TliA lipase, respectively, but had almost the same level of expression of TliD in the membrane as the strain with the wild-type TliDEF transporter [7].
  • When p-nitrophenyl esters and triglyceride substrates of various chain-lengths were examined, the lipase displayed highest activity towards C10 acyl groups [8].
  • Addition of EDTA completely abolished lipase activity, indicating that KB-Lip was a Ca2+-dependent lipase [8].

Chemical compound and disease context of PFL_0617


Biological context of PFL_0617


Anatomical context of PFL_0617


Associations of PFL_0617 with chemical compounds


Other interactions of PFL_0617

  • The lipA gene of Serratia marcescens which encodes an extracellular lipase having no N-terminal signal peptide [22].
  • In contrast, the production of another exoenzyme, lipase, is not regulated by the gacA gene [23].
  • Alignment of Pseudomonas fluorescens lipase/esterase-coding sequences allowed the design of specific primers for family VI lipases, and the isolation and cloning of the resulting gene estA6 [24].
  • Legionella pneumophila genes that encode lipase and phospholipase C activities [9].
  • In order to evaluate the pta(phosphotransacetylase) (-) mutant of Escherichia coli as a potential host of foreign lipase expression, the pta(-) mutant HB101 was constructed for the purpose of blocking the acetate synthetic pathway [25].

Analytical, diagnostic and therapeutic context of PFL_0617


  1. Serratia ATP-binding cassette protein exporter, Lip, recognizes a protein region upstream of the C terminus for specific secretion. Omori, K., Idei, A., Akatsuka, H. J. Biol. Chem. (2001) [Pubmed]
  2. The Pseudomonas fluorescens lipase has a C-terminal secretion signal and is secreted by a three-component bacterial ABC-exporter system. Duong, F., Soscia, C., Lazdunski, A., Murgier, M. Mol. Microbiol. (1994) [Pubmed]
  3. Gene cloning, sequence analysis, purification, and secretion by Escherichia coli of an extracellular lipase from Serratia marcescens. Li, X., Tetling, S., Winkler, U.K., Jaeger, K.E., Benedik, M.J. Appl. Environ. Microbiol. (1995) [Pubmed]
  4. Engineering of chimeric class II polyhydroxyalkanoate synthases. Niamsiri, N., Delamarre, S.C., Kim, Y.R., Batt, C.A. Appl. Environ. Microbiol. (2004) [Pubmed]
  5. Preliminary investigation of crystals of the neutral lipase from Pseudomonas fluorescens. Larson, S., Day, J., Greenwood, A., Oliver, J., Rubingh, D., McPherson, A. J. Mol. Biol. (1991) [Pubmed]
  6. Lipoprotein lipase enhances removal of chylomicrons and chylomicron remnants by the perfused rat liver. Skottova, N., Savonen, R., Lookene, A., Hultin, M., Olivecrona, G. J. Lipid Res. (1995) [Pubmed]
  7. Enhancement of the efficiency of secretion of heterologous lipase in Escherichia coli by directed evolution of the ABC transporter system. Eom, G.T., Song, J.K., Ahn, J.H., Seo, Y.S., Rhee, J.S. Appl. Environ. Microbiol. (2005) [Pubmed]
  8. Low-temperature lipase from psychrotrophic Pseudomonas sp. strain KB700A. Rashid, N., Shimada, Y., Ezaki, S., Atomi, H., Imanaka, T. Appl. Environ. Microbiol. (2001) [Pubmed]
  9. Legionella pneumophila genes that encode lipase and phospholipase C activities. Aragon, V., Rossier, O., Cianciotto, N.P. Microbiology (Reading, Engl.) (2002) [Pubmed]
  10. Use of immobilized lipases for lipase purification via specific lipase-lipase interactions. Palomo, J.M., Ortiz, C., Fuentes, M., Fernandez-Lorente, G., Guisan, J.M., Fernandez-Lafuente, R. Journal of chromatography. A. (2004) [Pubmed]
  11. Nondenaturing protein electrotransfer of the esterase activity of lipolytic preparations. Brahimi-Horn, M.C., Guglielmino, M.L., Gaal, A.M., Sparrow, L.G. Anal. Biochem. (1991) [Pubmed]
  12. Effect of Brij 58 on the hydrolysis of methyl butyrate by lipase from Pseudomonas fluorescens. Nakagawa, A., Tsujita, T., Okuda, H. J. Biochem. (1984) [Pubmed]
  13. Substrate specificities of bacterial polyhydroxyalkanoate depolymerases and lipases: bacterial lipases hydrolyze poly(omega-hydroxyalkanoates). Jaeger, K.E., Steinbüchel, A., Jendrossek, D. Appl. Environ. Microbiol. (1995) [Pubmed]
  14. Identification of the tliDEF ABC transporter specific for lipase in Pseudomonas fluorescens SIK W1. Ahn, J.H., Pan, J.G., Rhee, J.S. J. Bacteriol. (1999) [Pubmed]
  15. Cloning, expression, and nucleotide sequence of a lipase gene from Pseudomonas fluorescens B52. Tan, Y., Miller, K.J. Appl. Environ. Microbiol. (1992) [Pubmed]
  16. Molecular characterization of the extracellular poly(3-hydroxyoctanoic acid) [P(3HO)] depolymerase gene of Pseudomonas fluorescens GK13 and of its gene product. Schirmer, A., Jendrossek, D. J. Bacteriol. (1994) [Pubmed]
  17. Enzymatic desymmetrization of a meso polyol corresponding to the C(19)-C(27) segment of rifamycin S. Chênevert, R., Rose, Y.S. J. Org. Chem. (2000) [Pubmed]
  18. Determination of the extracellular lipases of Pseudomonas fluorescens spp. in skim milk with the beta-naphthyl caprylate assay. McKellar, R.C., Cholette, H. J. Dairy Res. (1986) [Pubmed]
  19. Wax ester-synthesizing activity of lipases. Tsujita, T., Sumiyoshi, M., Okuda, H. Lipids (1999) [Pubmed]
  20. Ester synthesis catalyzed by polyethylene glycol-modified lipase in benzene. Inada, Y., Nishimura, H., Takahashi, K., Yoshimoto, T., Saha, A.R., Saito, Y. Biochem. Biophys. Res. Commun. (1984) [Pubmed]
  21. Methyl-branched octanoic acids as substrates for lipase-catalyzed reactions. Sonnet, P.E., Baillargeon, M.W. Lipids (1991) [Pubmed]
  22. The lipA gene of Serratia marcescens which encodes an extracellular lipase having no N-terminal signal peptide. Akatsuka, H., Kawai, E., Omori, K., Komatsubara, S., Shibatani, T., Tosa, T. J. Bacteriol. (1994) [Pubmed]
  23. Extracellular protease and phospholipase C are controlled by the global regulatory gene gacA in the biocontrol strain Pseudomonas fluorescens CHA0. Sacherer, P., Défago, G., Haas, D. FEMS Microbiol. Lett. (1994) [Pubmed]
  24. Esterase EstA6 from Pseudomonas sp. CR-611 is a novel member in the utmost conserved cluster of family VI bacterial lipolytic enzymes. Prim, N., Bofill, C., Pastor, F.I., Diaz, P. Biochimie (2006) [Pubmed]
  25. Characterization and evaluation of a pta (phosphotransacetylase) negative mutant of Escherichia coli HB101 as production host of foreign lipase. Hahm, D.H., Pan, J., Rhee, J.S. Appl. Microbiol. Biotechnol. (1994) [Pubmed]
  26. Display of lipase on the cell surface of Escherichia coli using OprF as an anchor and its application to enantioselective resolution in organic solvent. Lee, S.H., Choi, J.I., Han, M.J., Choi, J.H., Lee, S.Y. Biotechnol. Bioeng. (2005) [Pubmed]
  27. Characterization of lipase of Pseudomonas fluorescens 27 based on fatty acid profiles. Ren, T.J., Frank, J.F., Christen, G.L. J. Dairy Sci. (1988) [Pubmed]
  28. Applications of immobilized lipases to transesterification and esterification reactions in nonaqueous systems. Mustranta, A., Forssell, P., Poutanen, K. Enzyme Microb. Technol. (1993) [Pubmed]
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