Disease relevance of LOC396462

• IgE antibodies to repurified ovomucoid were significantly greater in patients with persistent egg hypersensitivity compared with patients in whom clinical tolerance developed at the time of both initial and follow-up food challenges [1].
• Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I [2].
• Twelve recombinant phages carrying ovomucoid mRNA sequences were isolated [3].
• On the other hand, those residues that are in contact with the enzyme in turkey ovomucoid third domain complex with Streptomyces griseus proteinase B [Read, R., Fujinaga, M., Sielecki, A. R., & James, M. N. G. (1983) Biochemistry 22, 4420-4433] are not conserved but instead are by far the most variable residues in the molecule [4].
• BACKGROUND: Chicken ovomucoid (OM, Gal d 1) has an important role in the pathogenesis of IgE-mediated allergic reactions to hen's egg white [5].

High impact information on LOC396462

• Kinetic labeling and chase experiments in oviduct tissue-suspension system indicated that the ovalbumin and ovomucoid high molecular weight RNAs which were labeled with a short-time incubation can be chased by cold nucleosides and actinomycin D into mature mRNAs [6].
• Molecular structure and flanking nucleotide sequences of the natural chicken ovomucoid gene [7].
• Five independent clones containing the natural chicken ovomucoid gene have been isolated from a chicken gene library [7].
• Four globin genes alpha A, alpha D, beta, and rho or epsilon are located on at least two chromosomes, and three of the estrogen-inducible genes of the hen oviduct--ovalbumin, ovomucoid, and transferrin--are on three different chromosomes [8].
• Nonexchangeable proton and exchangeable amide (NH) proton resonances were assigned for the hen ovomucoid glycopeptide 1, Ser-Ile-Glu-Phe-Gly-Thr-Asn Ile-Ser-Lys, with pentasaccharide Man alpha 1-3 (Man alpha 1-6)Man beta 1-4GlcNAc beta 1-4GlcNAc beta 1-NH attached to the Asn7 gamma-carboxamide [9].

Chemical compound and disease context of LOC396462

• Ovomucoid is a major allergen in hen egg white which causes a serious IgE-mediated food allergy reaction [10].
• The various domains (domain I, domains II . III, domains I . II, and domain III with and without carbohydrate) were prepared from the ovomucoid by cyanogen bromide cleavage and Staphylococcus aureus protease V-8 digestions [11].
• Upon induction, the E. coli cells, harbouring this construct, expressed the recombinant protein as a soluble fraction and the recombinant ovomucoid protein was purified to electrophoeretic homogeneity using Ni2+ nitrilotriacetic acid agarose affinity chromatography [12].

Biological context of LOC396462

• One of these plasmids, pMu7, yielded the sequence of the 3'-untranslated region of ovomucoid mRNA [13].
• Nearly complete methylation of the lysines of lysozyme, chicken ovomucoid, and ribonuclease was achieved with formaldehyde at pH 7.0 after 2 h at room temperature, with the retention of full activity of the protein without any destruction of tryptophan [14].
• Effect of alkylation with different sized substituents on the conformation of ovomucoid, lysozyme and ovotransferrin [15].
• One of these clones, CL21, contains the complete ovomucoid gene and includes more than 3 kb of DNA sequences flanking both termini of the gene [7].
• Ovoinhibitor introns specify functional domains as in the related and linked ovomucoid gene [16].

Anatomical context of LOC396462

• Immunoadsorption of specific chicken oviduct polysomes. Isolation of ovalbumin, ovomucoid, and lysozyme messenger RNA [17].
• The largest RNAs labeled in this oviduct suspension system have a size of 7.8 kb for ovalbumin and 5.5 kb for ovomucoid, which correspond respectively to the "a" bands of steady state RNA [6].
• METHODS: Skin prick tests were performed with fresh white and yolk from eggs of duck and goose and egg white, egg yolk, ovalbumin, and ovomucoid from hen egg [18].
• Incubation of p erythrocytes in whole egg whites or in pigeon ovomucoid did not render them agglutinable by P-fimbriated bacteria, whereas incubation in globoside did [19].
• All of the precursors to ovalbumin and ovomucoid mRNAs (including various splicing intermediates) and all of the precursors to ribosomal RNA were associated exclusively with the nuclear matrix fraction [20].

Associations of LOC396462 with chemical compounds

• These results suggest that ovomucoid is the immunodominant protein fraction in egg white and that the use of commercially purified ovalbumin has led to an overestimation of the dominance of ovalbumin as a major egg allergen and antigen in human beings [1].
• On similar treatment no cleavage of the carbohydrate moiety from chicken ovomucoid, and no losses of activity of ovomucoid or ribonuclease, two proteins devoid of tryptophan, were observed [14].
• These results may be due primarily to the much higher solubility of ovomucoid in this aqueous ethanol [21].
• The antigenic and allergenic epitope maps of the hen egg-white ovalbumin, ovomucoid, ovotransferrin and lysozyme were obtained using one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis and two-dimensional sodium dodecyl sulfate gel electrophoresis following isoelectric focusing [22].
• In addition to these synergistic effects, dihydrotestosterone is capable of maintaining the synthesis of ovalbumin and ovomucoid mRNAs after estrogen withdrawal in vivo [23].

Other interactions of LOC396462

• From this library, ovomucoid cDNA and lysozyme cDNA-bearing plasmids have been identified [13].
• Commercially purified ovomucoid resulted in only minimal amounts of antibodies to ovalbumin [1].
• Thermodynamics of unfolding for Kazal-type serine protease inhibitors: entropic stabilization of ovomucoid first domain by glycosylation [24].
• Ligand blotting revealed significant differences in binding to two forms of the lectin only for glycoproteins bearing multiple N-linked oligosaccharide moieties in their molecule (riboflavin-binding glycoprotein, avidin or ovomucoid) [25].
• In this paper we report that serial hormone treatments also affect the rates of synthesis of two other egg white proteins, conalbumin and ovomucoid [26].

Analytical, diagnostic and therapeutic context of LOC396462

• Thus when commercially purified ovalbumin is used both for immunization and immunoassay, most of the antibodies produced are actually against the small amount of ovomucoid contaminant, and not ovalbumin [1].
• However, SDS-PAGE and immunoblot studies demonstrated contaminating lysozyme in the ovomucoid extract and identified lysozyme as the main allergen causing egg sensitization in this patient [27].
• The use of ELISA demonstrated that 70% of ovomucoid was recovered from the supernatant fraction [21].
• Conformational changes induced in ovomucoid, lysozyme and ovotransferrin on reductive addition of different sized substituents have been studied employing differential scanning calorimetry (DSC) and circular dichroic spectroscopy (CD) [15].
• Restriction endonuclease mapping, electron microscopy and direct DNA sequencing analyses of this clone have revealed that the ovomucoid gene is 5.6 kb long and codes for a messenger RNA of 821 nucleotides [7].

References