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Gene Review

TACSTD2  -  tumor-associated calcium signal transducer 2

Homo sapiens

Synonyms: Cell surface glycoprotein Trop-2, EGP-1, EGP1, GA733-1, GA7331, ...
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Disease relevance of TACSTD2


High impact information on TACSTD2

  • Colorectal carcinoma invasion inhibition by CO17-1A/GA733 antigen and its murine homologue [3].
  • The availability of a murine homologue of GA733, i.e., murine epithelial glycoprotein (mEGP), allows for functional analysis of cell adhesion as it relates to tumor growth and invasion, both in vitro and in vivo [3].
  • GA733- or mEGP-producing cells were evaluated for homophilic adhesion, growth on plastic surfaces, colony formation in soft agar, and invasion through a reconstructed basement membrane (Matrigel). mEGP-producing cells were also examined for their capacity to metastasize in mice [3].
  • The positions of 12 cysteine residues in the extracellular domains of the two GA733 antigens are conserved, as is the overall distribution of hydrophobic and hydrophilic residues [2].
  • The monoclonal antibody-defined, tumor-associated antigen GA733 was purified from the SW948 human colorectal carcinoma cell line and its partial amino acid sequence was determined [5].

Chemical compound and disease context of TACSTD2

  • Two internalizing mAbs, LL2 (anti-CD 22 B-cell lymphoma mAb) and RS7 (an anti-adenocarcinoma mAb which targets EGP-1 antigen), labeled with this procedure exhibited a 2-3-fold better cellular retention in Ramos and Calu-3 tumor cell lines, in vitro, respectively, compared to the same mAbs radioiodinated with the chloramine-T method [6].

Biological context of TACSTD2


Anatomical context of TACSTD2

  • A 1.1-kb DNA fragment of the TACSTD2 gene was amplified and analyzed using a molecular biological method. cDNA from the patient's cornea was also analyzed to determine which allele was expressed in the patient's corneal epithelium [7].
  • Trop-1 and Trop-2 appear to be specifically expressed on syncytio- and cytotrophoblasts, whereas Trop-3 and Trop-4 are also detected on various tumor cell lines, normal lymphocytes, and monocytes [10].
  • The sequence of the epithelial glycoprotein antigen is identical to the cell membrane protein recognized by the monoclonal antibody KS 1/4 and is homologous to the tumor-associated antigen GA733 [11].
  • Immunohistoperoxidase staining with polyclonal rabbit antibodies to the human antigen revealed the human homologue on normal small intestine, colon and liver of mice, rats and non-human primates, whereas mouse monoclonal antibodies to the CO17-1A or GA733 epitopes on the human antigen did not detect the antigen [12].
  • Chimeric T cell receptors were constructed by ligating single-chain genes of either of two EGP40-specific monoclonal antibodies (CO17.1 A, GA733) to the Fc receptor gamma-signaling chain [13].

Associations of TACSTD2 with chemical compounds

  • We propose to call the antigen detected by SCLC-cluster-2 antibodies "epithelial glycoprotein 2" (EGP-2), and the epithelium-associated glycoprotein recognized by antibodies clustered in SCLC cluster 13 (see elsewhere in this volume) "epithelial glycoprotein 1" (EGP-1) [14].
  • Very close to the location where the mutation was identified in the M1S1 gene, a single-nucleotide polymorphism (518A/C) was found, changing aspartic acid to alanine at codon 173 [15].
  • The rate constant for carbon monoxide binding to this form of hemoglobin is 4.1 X 10(6) M-1 S-1 at 24 degrees in our solutions [16].
  • The equilibrium constant for CO binding to the ferrous hemeprotein was measured to be 1.7 X 10(-5) M-1 and the CO association rate constant determined to be 5.4 X 10(3) M-1 S-1 [17].
  • The second order rate constant (Kf) for the formation of Compound X at pH 4.5 (optimum pH) is 0.9 X 10(6) M-1 S-1 [18].

Regulatory relationships of TACSTD2

  • Ad5 GA733-2 induced Ag-specific Abs that reacted predominantly to CO17-1A- and GA733-unrelated epitopes on the Ag and lysed Ag-positive CRC targets in conjunction with effector cells [4].
  • Kinetic analyses revealed that factor VII activation in the presence of relipidated tissue factor apoprotein can be defined by a second-order reaction mechanism in which factor VII is activated by factor VIIa with an apparent second-order rate constant of 7.2 x 10(3) M-1 S-1 [19].

Other interactions of TACSTD2


Analytical, diagnostic and therapeutic context of TACSTD2


  1. Establishment of an immortalized cell line from a precancerous lesion of lung adenocarcinoma, and genes highly expressed in the early stages of lung adenocarcinoma development. Shimada, A., Kano, J., Ishiyama, T., Okubo, C., Iijima, T., Morishita, Y., Minami, Y., Inadome, Y., Shu, Y., Sugita, S., Takeuchi, T., Noguchi, M. Cancer Sci. (2005) [Pubmed]
  2. Molecular cloning of cDNA for the carcinoma-associated antigen GA733-2. Szala, S., Froehlich, M., Scollon, M., Kasai, Y., Steplewski, Z., Koprowski, H., Linnenbach, A.J. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  3. Colorectal carcinoma invasion inhibition by CO17-1A/GA733 antigen and its murine homologue. Basak, S., Speicher, D., Eck, S., Wunner, W., Maul, G., Simmons, M.S., Herlyn, D. J. Natl. Cancer Inst. (1998) [Pubmed]
  4. Human colorectal cancer (CRC) antigen CO17-1A/GA733 encoded by adenovirus inhibits growth of established CRC cells in mice. Li, W., Berencsi, K., Basak, S., Somasundaram, R., Ricciardi, R.P., Gönczöl, E., Zaloudik, J., Linnenbach, A., Maruyama, H., Miniou, P., Herlyn, D. J. Immunol. (1997) [Pubmed]
  5. Sequence investigation of the major gastrointestinal tumor-associated antigen gene family, GA733. Linnenbach, A.J., Wojcierowski, J., Wu, S.A., Pyrc, J.J., Ross, A.H., Dietzschold, B., Speicher, D., Koprowski, H. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  6. Labeling of monoclonal antibodies with diethylenetriaminepentaacetic acid-appended radioiodinated peptides containing D-amino acids. Govindan, S.V., Mattes, M.J., Stein, R., McBride, B.J., Karacay, H., Goldenberg, D.M., Hansen, H.J., Griffiths, G.L. Bioconjug. Chem. (1999) [Pubmed]
  7. A novel missense mutation in a Japanese patient with gelatinous droplike corneal dystrophy. Taniguchi, Y., Tsujikawa, M., Hibino, S., Tsujikawa, K., Tanaka, T., Kiridoushi, A., Tano, Y. Am. J. Ophthalmol. (2005) [Pubmed]
  8. Human Trop-2 is a tumor-associated calcium signal transducer. Ripani, E., Sacchetti, A., Corda, D., Alberti, S. Int. J. Cancer (1998) [Pubmed]
  9. Cancer vaccines: single-epitope anti-idiotype vaccine versus multiple-epitope antigen vaccine. Maruyama, H., Zaloudik, J., Li, W., Sperlagh, M., Koido, T., Somasundaram, R., Scheck, S., Prewett, M., Herlyn, D. Cancer Immunol. Immunother. (2000) [Pubmed]
  10. Human trophoblast cell-surface antigens defined by monoclonal antibodies. Lipinski, M., Parks, D.R., Rouse, R.V., Herzenberg, L.A. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]
  11. Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein. Simon, B., Podolsky, D.K., Moldenhauer, G., Isselbacher, K.J., Gattoni-Celli, S., Brand, S.J. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  12. Expression of an antigen homologous to the human CO17-1A/GA733 colon cancer antigen in animal tissues. Zaloudik, J., Basak, S., Nesbit, M., Speicher, D.W., Wunner, W.H., Miller, E., Ernst-Grotkowski, C., Kennedy, R., Bergsagel, L.P., Koido, T., Herlyn, D. Br. J. Cancer (1997) [Pubmed]
  13. Recognition of human colon cancer by T cells transduced with a chimeric receptor gene. Daly, T., Royal, R.E., Kershaw, M.H., Treisman, J., Wang, G., Li, W., Herlyn, D., Eshhar, Z., Hwu, P. Cancer Gene Ther. (2000) [Pubmed]
  14. SCLC-cluster-2 antibodies detect the pancarcinoma/epithelial glycoprotein EGP-2. De Leij, L., Helrich, W., Stein, R., Mattes, M.J. Int. J. Cancer Suppl. (1994) [Pubmed]
  15. A novel mutation in the M1S1 gene responsible for gelatinous droplike corneal dystrophy. Tasa, G., Kals, J., Muru, K., Juronen, E., Piirsoo, A., Veromann, S., Jänes, S., Mikelsaar, A.V., Lang, A. Invest. Ophthalmol. Vis. Sci. (2001) [Pubmed]
  16. Kinetics of carbon monoxide binding to singly reduced human methemoglobin. Ho, K., Klapper, M.H., Dorfman, L.M. J. Biol. Chem. (1978) [Pubmed]
  17. Spectroscopic and kinetic properties of an oxygen-binding heme protein from Chromatium vinosum. Gaul, D.F., Ondrias, M.R., Findsen, E.W., Palmer, G., Olson, J.S., Davidson, M.W., Knaff, D.B. J. Biol. Chem. (1987) [Pubmed]
  18. Compound X. An intermediate in enzymatic halogenation. Chiang, R., Rand-Meir, T., Makino, R., Hager, L.P. J. Biol. Chem. (1976) [Pubmed]
  19. Tissue factor-dependent autoactivation of human blood coagulation factor VII. Yamamoto, M., Nakagaki, T., Kisiel, W. J. Biol. Chem. (1992) [Pubmed]
  20. Cloning of the gene encoding Trop-2, a cell-surface glycoprotein expressed by human carcinomas. Fornaro, M., Dell'Arciprete, R., Stella, M., Bucci, C., Nutini, M., Capri, M.G., Alberti, S. Int. J. Cancer (1995) [Pubmed]
  21. Cyclin D1 gene contains a cryptic promoter that is functional in human cancer cells. Terrinoni, A., Dell'Arciprete, R., Fornaro, M., Stella, M., Alberti, S. Genes Chromosomes Cancer (2001) [Pubmed]
  22. Immunotherapy in pancreatic cancer - current status and future. Staib, L., Link, K.H., Beger, H.G. Langenbeck's archives of surgery / Deutsche Gesellschaft für Chirurgie. (1999) [Pubmed]
  23. A novel TACSTD2 gene mutation in a Turkish family with a gelatinous drop-like corneal dystrophy. Markoff, A., Bogdanova, N., Uhlig, C.E., Groppe, M., Horst, J., Kennerknecht, I. Mol. Vis. (2006) [Pubmed]
  24. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody. Björk, P., Jönsson, U., Svedberg, H., Larsson, K., Lind, P., Dillner, J., Hedlund, G., Dohlsten, M., Kalland, T. J. Biol. Chem. (1993) [Pubmed]
  25. EpCAM expression in retinoblastoma: a novel molecular target for therapy. Krishnakumar, S., Mohan, A., Mallikarjuna, K., Venkatesan, N., Biswas, J., Shanmugam, M.P., Ren-Heidenreich, L. Invest. Ophthalmol. Vis. Sci. (2004) [Pubmed]
  26. Mutations in the membrane component, chromosome 1, surface marker 1 (M1S1) gene in gelatinous drop-like corneal dystrophy. Murakami, A., Kimura, S., Fujiki, K., Fujimaki, T., Kanai, A. Jpn. J. Ophthalmol. (2004) [Pubmed]
  27. Induction of antigen-specific T and B cell immunity in colon carcinoma patients by anti-idiotypic antibody. Somasundaram, R., Zaloudik, J., Jacob, L., Benden, A., Sperlagh, M., Hart, E., Marks, G., Kane, M., Mastrangelo, M., Herlyn, D. J. Immunol. (1995) [Pubmed]
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