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Gene Review

RAD51B  -  RAD51 paralog B

Homo sapiens

Synonyms: DNA repair protein RAD51 homolog 2, R51H2, RAD51 homolog B, RAD51-like protein 1, RAD51L1, ...
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Disease relevance of RAD51L1


High impact information on RAD51L1


Biological context of RAD51L1


Anatomical context of RAD51L1


Associations of RAD51L1 with chemical compounds

  • Here, we report the results of studies on the behavior of CHO cells containing a plasmid encoding a wild-type hsRec2/Rad51B, a full-length protein with a single mutation at residue 163, which lies in the putative src site, and a truncated version of hsRec2/Rad51B, containing only the first 100 amino acids at the NH2 terminus [7].
  • RAD51B(-/-) cells are quite sensitive to the cross-linking agents cisplatin and mitomycin C and mildly sensitive to gamma-rays [4].
  • In the present study, we found that the purified Rad51B protein bound to single-stranded DNA and double-stranded DNA in the presence of ATP and either Mg(2+) or Mn(2+) and hydrolyzed ATP in a DNA-dependent manner [12].

Physical interactions of RAD51L1

  • We extended these observations to examine the interaction between the RAD51B/RAD51C complex and the other RAD51 paralogs [13].
  • For example, Rad51B expression enhances the binding of Rad51C to XRCC3 and to HsRad51D, and Rad51C expression allows the indirect interaction of Rad51B with Rad51D [14].

Regulatory relationships of RAD51L1

  • Experiments using 6xHis-tagged proteins in the baculovirus system confirm several of our yeast results, including Rad51B interaction with Rad51D only when Rad51C is simultaneously expressed and Rad51C interaction with XRCC2 only when Rad51D is present [14].

Other interactions of RAD51L1

  • RAD51L1-HMGA2 transcripts were not detected [15].
  • The RAD51B/RAD51C heterocomplex was isolated and purified by immunoaffinity chromatography from insect cells co-expressing the recombinant proteins [13].
  • In addition, we find that Rad51C, but not XRCC3, interacts directly or indirectly with Rad51B, Rad51D and XRCC2 [16].
  • In addition, Rad51B could be pulled down with Rad51C and Rad51D, but not with XRCC3 [17].
  • Nuclear localization of Rad51B is independent of Rad51C and BRCA2 [18].

Analytical, diagnostic and therapeutic context of RAD51L1


  1. Haploinsufficiency of RAD51B causes centrosome fragmentation and aneuploidy in human cells. Date, O., Katsura, M., Ishida, M., Yoshihara, T., Kinomura, A., Sueda, T., Miyagawa, K. Cancer Res. (2006) [Pubmed]
  2. Familial occurrence of thymoma and autoimmune diseases with the constitutional translocation t(14;20)(q24.1;p12.3). Nicodème, F., Geffroy, S., Conti, M., Delobel, B., Soenen, V., Grardel, N., Porte, H., Copin, M.C., Laï, J.L., Andrieux, J. Genes Chromosomes Cancer (2005) [Pubmed]
  3. A surfeit of RAD51-like genes? Thacker, J. Trends Genet. (1999) [Pubmed]
  4. The Rad51 paralog Rad51B promotes homologous recombinational repair. Takata, M., Sasaki, M.S., Sonoda, E., Fukushima, T., Morrison, C., Albala, J.S., Swagemakers, S.M., Kanaar, R., Thompson, L.H., Takeda, S. Mol. Cell. Biol. (2000) [Pubmed]
  5. The Polycomb group protein EZH2 impairs DNA repair in breast epithelial cells. Zeidler, M., Varambally, S., Cao, Q., Chinnaiyan, A.M., Ferguson, D.O., Merajver, S.D., Kleer, C.G. Neoplasia (2005) [Pubmed]
  6. Evidence for RAD51L1/HMGIC fusion in the pathogenesis of uterine leiomyoma. Takahashi, T., Nagai, N., Oda, H., Ohama, K., Kamada, N., Miyagawa, K. Genes Chromosomes Cancer (2001) [Pubmed]
  7. The human REC2/RAD51B gene acts as a DNA damage sensor by inducing G1 delay and hypersensitivity to ultraviolet irradiation. Havre, P.A., Rice, M.C., Noe, M., Kmiec, E.B. Cancer Res. (1998) [Pubmed]
  8. Allelic knockout of novel splice variants of human recombination repair gene RAD51B in t(12;14) uterine leiomyomas. Schoenmakers, E.F., Huysmans, C., Van de Ven, W.J. Cancer Res. (1999) [Pubmed]
  9. XRCC3 ATPase activity is required for normal XRCC3-Rad51C complex dynamics and homologous recombination. Yamada, N.A., Hinz, J.M., Kopf, V.L., Segalle, K.D., Thompson, L.H. J. Biol. Chem. (2004) [Pubmed]
  10. Identification of a novel human RAD51 homolog, RAD51B. Albala, J.S., Thelen, M.P., Prange, C., Fan, W., Christensen, M., Thompson, L.H., Lennon, G.G. Genomics (1997) [Pubmed]
  11. hREC2, a RAD51-like gene, is disrupted by t(12;14) (q15;q24.1) in a uterine leiomyoma. Ingraham, S.E., Lynch, R.A., Kathiresan, S., Buckler, A.J., Menon, A.G. Cancer Genet. Cytogenet. (1999) [Pubmed]
  12. Holliday junction binding activity of the human Rad51B protein. Yokoyama, H., Kurumizaka, H., Ikawa, S., Yokoyama, S., Shibata, T. J. Biol. Chem. (2003) [Pubmed]
  13. RAD51C interacts with RAD51B and is central to a larger protein complex in vivo exclusive of RAD51. Miller, K.A., Yoshikawa, D.M., McConnell, I.R., Clark, R., Schild, D., Albala, J.S. J. Biol. Chem. (2002) [Pubmed]
  14. Evidence for simultaneous protein interactions between human Rad51 paralogs. Schild, D., Lio, Y.C., Collins, D.W., Tsomondo, T., Chen, D.J. J. Biol. Chem. (2000) [Pubmed]
  15. Fusion transcripts involving HMGA2 are not a common molecular mechanism in uterine leiomyomata with rearrangements in 12q15. Quade, B.J., Weremowicz, S., Neskey, D.M., Vanni, R., Ladd, C., Dal Cin, P., Morton, C.C. Cancer Res. (2003) [Pubmed]
  16. Interactions involving the Rad51 paralogs Rad51C and XRCC3 in human cells. Wiese, C., Collins, D.W., Albala, J.S., Thompson, L.H., Kronenberg, A., Schild, D. Nucleic Acids Res. (2002) [Pubmed]
  17. Involvement of Rad51C in two distinct protein complexes of Rad51 paralogs in human cells. Liu, N., Schild, D., Thelen, M.P., Thompson, L.H. Nucleic Acids Res. (2002) [Pubmed]
  18. Nuclear localization of Rad51B is independent of Rad51C and BRCA2. Miller, K.A., Hinz, J.M., Yamada, N.A., Thompson, L.H., Albala, J.S. Mutagenesis (2005) [Pubmed]
  19. Isolation of human and mouse genes based on homology to REC2, a recombinational repair gene from the fungus Ustilago maydis. Rice, M.C., Smith, S.T., Bullrich, F., Havre, P., Kmiec, E.B. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
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